GPR62 (NM_080865) Human Tagged ORF Clone

CAT#: RG205539

  • TrueORF®

GPR62 (tGFP-tagged) - Human G protein-coupled receptor 62 (GPR62)

ORF Plasmid: DDK tGFP



  "NM_080865" in other vectors (4)

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CNY 4,370.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


GPR62 rabbit polyclonal antibody
    • 25 ul

CNY 800.00
CNY 1,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms GPCR8; KPG_005
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG205539 representing NM_080865
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAACTCCACAGGGCTGAACGCCTCAGAAGTCGCAGGCTCGTTGGGGTTGATCCTGGCAGCTGTCG
TGGAGGTGGGGGCACTGCTGGGCAACGGCGCGCTGCTGGTCGTGGTGCTGCGCACGCCGGGACTGCGCGA
CGCGCTCTACCTGGCGCACCTGTGCGTCGTGGACCTGCTGGCGGCCGCCTCCATCATGCCGCTGGGCCTG
CTGGCCGCACCGCCGCCCGGGCTGGGCCGCGTGCGCCTGGGCCCCGCGCCATGCCGCGCCGCTCGCTTCC
TCTCCGCCGCTCTGCTGCCGGCCTGCACGCTCGGGGTGGCCGCACTTGGCCTGGCACGCTACCGCCTCAT
CGTGCACCCGCTGCGGCCAGGCTCGCGGCCGCCGCCTGTGCTCGTGCTCACCGCCGTGTGGGCCGCGGCG
GGACTGCTGGGCGCGCTCTCCCTGCTCGGCACGCCGCCCGCACCGCCCCCTGCTCCTGCTCGCTGCTCGG
TCCTGGCTGGGGGCCTCGGGCCCTTCCGGCCGCTCTGGGCCCTGCTGGCCTTCGCGCTGCCCGCCCTCCT
GCTGCTCGGCGCCTACGGCGGCATCTTCGTGGTGGCGCGTCGCGCTGCCCTGAGGCCCCCACGGCCGGCG
CGCGGGTCCCGACTCCACTCGGACTCTCTGGATAGCCGCCTTTCCATCTTGCCGCCGCTCCGGCCTCGCC
TGCCCGGGGGCAAGGCGGCCCTGGCCCCAGCGCTGGCCGTGGGCCAATTTGCAGCCTGCTGGCTGCCTTA
TGGCTGCGCGTGCCTGGCGCCCGCAGCGCGGGCCGCGGAAGCCGAAGCGGCTGTCACCTGGGTCGCCTAC
TCGGCCTTCGCGGCTCACCCCTTCCTGTACGGGCTGCTGCAGCGCCCCGTGCGCTTGGCACTGGGCCGCC
TCTCTCGCCGTGCACTGCCTGGACCTGTGCGGGCCTGCACTCCGCAAGCCTGGCACCCGCGGGCACTCTT
GCAATGCCTCCAGAGACCCCCAGAGGGCCCTGCCGTAGGCCCTTCTGAGGCTCCAGAACAGACCCCCGAG
TTGGCAGGAGGGCGGAGCCCCGCATACCAGGGGCCACCTGAGAGTTCTCTCTCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG205539 representing NM_080865
Red=Cloning site Green=Tags(s)

MANSTGLNASEVAGSLGLILAAVVEVGALLGNGALLVVVLRTPGLRDALYLAHLCVVDLLAAASIMPLGL
LAAPPPGLGRVRLGPAPCRAARFLSAALLPACTLGVAALGLARYRLIVHPLRPGSRPPPVLVLTAVWAAA
GLLGALSLLGTPPAPPPAPARCSVLAGGLGPFRPLWALLAFALPALLLLGAYGGIFVVARRAALRPPRPA
RGSRLHSDSLDSRLSILPPLRPRLPGGKAALAPALAVGQFAACWLPYGCACLAPAARAAEAEAAVTWVAY
SAFAAHPFLYGLLQRPVRLALGRLSRRALPGPVRACTPQAWHPRALLQCLQRPPEGPAVGPSEAPEQTPE
LAGGRSPAYQGPPESSLS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_080865
ORF Size 1104 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_080865.4
RefSeq Size 2206 bp
RefSeq ORF 1107 bp
Locus ID 118442
UniProt ID Q9BZJ7
Protein Families Druggable Genome, GPCR, Transmembrane
Gene Summary Orphan G-protein coupled receptor. Constitutively activates the G(q/11)/inositol phosphate and the G(s)-alpha/cAMP signaling pathways (PubMed:28827538). Has spontaneous activity for beta-arrestin recruitment (PubMed:28827538). Shows a reciprocal modulation of signaling functions with the melatonin receptor MTNR1B most likely through receptor heteromerization (PubMed:28827538).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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