DIRAS1 (NM_145173) Human Tagged ORF Clone
CAT#: RG206160
- TrueORF®
DIRAS1 (tGFP-tagged) - Human DIRAS family, GTP-binding RAS-like 1 (DIRAS1)
ORF Plasmid: DDK
"NM_145173" in other vectors (4)
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CNY 4,000.00
CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Di-Ras1; GBTS1; RIG |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG206160 representing NM_145173
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGAACAGAGTAACGATTACCGCGTGGTGGTGTTCGGGGCGGGCGGCGTGGGCAAGAGCTCGCTGG TGCTGCGCTTCGTGAAGGGCACGTTCCGCGACACCTACATCCCCACCATCGAGGACACCTACCGGCAGGT GATCAGCTGCGACAAGAGCGTGTGCACGCTGCAGATCACAGACACCACCGGCAGCCACCAGTTCCCGGCC ATGCAGCGCCTGTCCATCTCCAAGGGCCACGCCTTCATCCTGGTGTTCTCCGTCACCAGCAAGCAGTCGC TGGAGGAGCTGGGGCCCATCTACAAGCTCATCGTGCAGATCAAGGGCAGCGTGGAGGACATCCCCGTGAT GCTCGTGGGCAACAAGTGCGATGAGACGCAGCGGGAGGTGGACACGCGCGAGGCGCAGGCGGTGGCCCAG GAGTGGAAGTGCGCTTTCATGGAGACCTCGGCCAAGATGAACTACAACGTCAAGGAGCTCTTCCAGGAGC TGCTGACGCTGGAGACGCGCCGGAACATGAGCCTCAACATCGACGGCAAGCGCTCCGGGAAGCAGAAGAG GACAGACCGCGTCAAGGGCAAATGCACCCTCATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG206160 representing NM_145173
Red=Cloning site Green=Tags(s) MPEQSNDYRVVVFGAGGVGKSSLVLRFVKGTFRDTYIPTIEDTYRQVISCDKSVCTLQITDTTGSHQFPA MQRLSISKGHAFILVFSVTSKQSLEELGPIYKLIVQIKGSVEDIPVMLVGNKCDETQREVDTREAQAVAQ EWKCAFMETSAKMNYNVKELFQELLTLETRRNMSLNIDGKRSGKQKRTDRVKGKCTLM TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_145173 |
ORF Size | 594 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_145173.4 |
RefSeq Size | 3391 bp |
RefSeq ORF | 597 bp |
Locus ID | 148252 |
UniProt ID | O95057 |
Domains | ras, RAS, RHO, RAB |
Gene Summary | DIRAS1 belongs to a distinct branch of the functionally diverse Ras (see HRAS; MIM 190020) superfamily of monomeric GTPases.[supplied by OMIM, Apr 2004] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC206160 | DIRAS1 (Myc-DDK-tagged)-Human DIRAS family, GTP-binding RAS-like 1 (DIRAS1) |
CNY 2,400.00 |
|
RC206160L3 | Lenti ORF clone of Human DIRAS family, GTP-binding RAS-like 1 (DIRAS1), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC206160L4 | Lenti ORF clone of Human DIRAS family, GTP-binding RAS-like 1 (DIRAS1), mGFP tagged |
CNY 5,890.00 |
|
SC100097 | DIRAS1 (untagged)-Human DIRAS family, GTP-binding RAS-like 1 (DIRAS1) |
CNY 2,400.00 |