United States
Germany
Japan
United Kingdom
China
GRAMD1C (NM_017577) Human Tagged ORF Clone
CAT＃: RG206376
- TrueORF®
GRAMD1C (tGFP-tagged) - Human GRAM domain containing 1C (GRAMD1C), transcript variant 1
ORF Plasmid: DDK
"NM_017577" in other vectors (5)
Need custom modification / cloning service?
Get a free quote
CNY 7,024.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG206376 representing NM_017577
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGCGCTCCGACTGTCCGTCAGGTGATGAATGAAGGGGATTCAAGCCTTGCCACCGACTTACAGG AAGATGTAGAGGAAAATCCTAGTCCAACTGTGGAAGAGAATAATGTGGTAGTTAAAAAACAGGGGCCAAA TTTACATAATTGGAGTGGTGACTGGAGCTTTTGGATTTCAAGTTCCACCTATAAAGACAGGAATGAGGAA TACAGAAGACAGTTCACACATCTACCTGATACAGAGAGGCTGATAGCAGATTATGCTTGTGCTCTTCAGA GGGACATTTTGCTTCAGGGACGACTATACCTTTCAGAAAACTGGCTATGTTTCTATAGCAACATCTTCAG ATGGGAAACTACAATTTCTATTGCTTTAAAGAATATAACCTTCATGACCAAGGAAAAAACTGCTCGACTC ATCCCAAACGCTATCCAGATAGTTACAGAAAGTGAAAAGTTTTTCTTCACATCTTTTGGTGCCAGGGATA GAAGTTACCTCAGTATCTTTAGGTTGTGGCAGAATGTATTATTAGATAAGAGCCTGACTAGACAGGAATT CTGGCAACTGCTCCAGCAGAACTATGGCACTGAGCTAGGTTTAAATGCTGAGGAGATGGAAAACTTGTCA CTGTCGATTGAGGATGTGCAGCCAAGAAGTCCAGGAAGAAGCAGCTTGGATGACTCTGGGGAGAGAGATG AAAAATTATCCAAGTCAATCAGTTTTACCAGTGAATCAATTAGTCGGGTTTCAGAAACAGAGTCATTCGA TGGAAATTCATCAAAAGGAGGATTAGGCAAAGAGGAGTCCCAAAATGAGAAACAGACCAAAAAGAGTCTC TTACCAACTTTGGAAAAGAAGTTAACTAGAGTGCCATCAAAGTCACTGGACTTGAATAAAAATGAATATC TTTCTCTGGACAAAAGCAGCACTTCAGATTCTGTTGATGAAGAAAATGTTCCTGAGAAAGATCTTCATGG AAGACTTTTTATCAACCGTATTTTTCATATCAGTGCTGACAGAATGTTTGAATTGCTCTTTACCAGTTCA CGCTTTATGCAGAAATTTGCCAGTTCTAGAAATATAATAGATGTAGTATCTACCCCTTGGACTGCAGAAC TTGGAGGTGATCAGCTGAGAACGATGACCTACACTATAGTCCTTAATAGTCCACTTACTGGAAAATGCAC TGCTGCCACTGAAAAGCAGACACTGTATAAAGAAAGTCGGGAAGCACGATTTTATTTGGTAGATTCAGAA GTACTGACACATGATGTCCCCTACCATGATTACTTCTATACCGTGAACAGATACTGTATCATCCGATCTT CAAAACAGAAATGCAGGCTAAGAGTTTCCACAGATTTGAAATACAGAAAACAGCCATGGGGCCTTGTCAA ATCTTTAATTGAAAAGAATTCCTGGAGTTCTTTGGAGGACTATTTCAAACAGCTTGAATCAGATTTGTTA ATTGAAGAATCTGTATTAAATCAGGCCATTGAAGACCCTGGAAAACTTACTGGCCTACGAAGGAGAAGGC GAACCTTCAACCGAACAGCAGAAACAGTTCCTAAACTTTCCTCTCAGCATTCCTCTGGAGATGTGGGCTT AGGTGCCAAAGGGGATATTACAGGAAAGAAAAAGGAAATGGAAAACTATAACGTCACTCTTATTGTGGTA ATGAGTATTTTTGTGTTGTTATTAGTTTTGTTGAATGTGACACTGTTTCTGAAGCTGTCAAAGATAGAAC ATGCTGCTCAGTCCTTTTACCGTCTCCGCCTCCAAGAAGAGAAATCTTTAAATTTAGCCTCTGATATGGT GTCAAGAGCAGAAACTATTCAGAAGAATAAAGATCAGGCCCATCGTTTAAAGGGAGTGCTCCGAGACTCC ATAGTGATGCTTGAACAGCTGAAGAGCTCACTCATTATGCTTCAGAAAACGTTTGATCTACTAAATAAGA ATAAGACTGGCATGGCTGTTGAAAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG206376 representing NM_017577
Red=Cloning site Green=Tags(s) MEGAPTVRQVMNEGDSSLATDLQEDVEENPSPTVEENNVVVKKQGPNLHNWSGDWSFWISSSTYKDRNEE YRRQFTHLPDTERLIADYACALQRDILLQGRLYLSENWLCFYSNIFRWETTISIALKNITFMTKEKTARL IPNAIQIVTESEKFFFTSFGARDRSYLSIFRLWQNVLLDKSLTRQEFWQLLQQNYGTELGLNAEEMENLS LSIEDVQPRSPGRSSLDDSGERDEKLSKSISFTSESISRVSETESFDGNSSKGGLGKEESQNEKQTKKSL LPTLEKKLTRVPSKSLDLNKNEYLSLDKSSTSDSVDEENVPEKDLHGRLFINRIFHISADRMFELLFTSS RFMQKFASSRNIIDVVSTPWTAELGGDQLRTMTYTIVLNSPLTGKCTAATEKQTLYKESREARFYLVDSE VLTHDVPYHDYFYTVNRYCIIRSSKQKCRLRVSTDLKYRKQPWGLVKSLIEKNSWSSLEDYFKQLESDLL IEESVLNQAIEDPGKLTGLRRRRRTFNRTAETVPKLSSQHSSGDVGLGAKGDITGKKKEMENYNVTLIVV MSIFVLLLVLLNVTLFLKLSKIEHAAQSFYRLRLQEEKSLNLASDMVSRAETIQKNKDQAHRLKGVLRDS IVMLEQLKSSLIMLQKTFDLLNKNKTGMAVES TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017577 |
| ORF Size | 1986 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017577.5 |
| RefSeq Size | 3770 bp |
| RefSeq ORF | 1989 bp |
| Locus ID | 54762 |
| UniProt ID | Q8IYS0 |
| Protein Families | Transmembrane |
| Gene Summary | Cholesterol transporter that mediates non-vesicular transport of cholesterol from the plasma membrane (PM) to the endoplasmic reticulum (ER) (By similarity). Contains unique domains for binding cholesterol and the PM, thereby serving as a molecular bridge for the transfer of cholesterol from the PM to the ER (By similarity). Plays a crucial role in cholesterol homeostasis and has the unique ability to localize to the PM based on the level of membrane cholesterol (By similarity). In lipid-poor conditions localizes to the ER membrane and in response to excess cholesterol in the PM is recruited to the endoplasmic reticulum-plasma membrane contact sites (EPCS) which is mediated by the GRAM domain (By similarity). At the EPCS, the sterol-binding VASt/ASTER domain binds to the cholesterol in the PM and facilitates its transfer from the PM to ER (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206376 | GRAMD1C (Myc-DDK-tagged)-Human GRAM domain containing 1C (GRAMD1C), transcript variant 1 |
CNY 3,990.00 |
|
| RC206376L3 | Lenti-ORF clone of GRAMD1C (Myc-DDK-tagged)-Human GRAM domain containing 1C (GRAMD1C), transcript variant 1 |
CNY 5,890.00 |
|
| RC206376L4 | Lenti-ORF clone of GRAMD1C (mGFP-tagged)-Human GRAM domain containing 1C (GRAMD1C), transcript variant 1 |
CNY 5,890.00 |
|
| SC108634 | GRAMD1C (untagged)-Human GRAM domain containing 1C (GRAMD1C), transcript variant 1 |
CNY 2,400.00 |
|
| SC318125 | GRAMD1C (untagged)-Human GRAM domain containing 1C (GRAMD1C), transcript variant 1 |
CNY 7,220.00 |
其它GRAMD1C产品
