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SPINLW1 (EPPIN) (NM_020398) Human Tagged ORF Clone
CAT＃: RG206458
- TrueORF®
EPPIN (tGFP-tagged) - Human serine peptidase inhibitor-like, with Kunitz and WAP domains 1 (eppin) (SPINLW1)
ORF Plasmid: DDK
"NM_020398" in other vectors (4)
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CNY 2,800.00
CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CT71; CT72; dJ461P17.2; SPINLW1; WAP7; WFDC7 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG206458 representing NM_020398
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGATCTTCTGGACTTTTGAGCCTCCTGGTGCTATTCGTCCTCTTAGCGAATGTCCAGGGACCTGGTC TGACTGATTGGTTATTTCCCAGGAGATGTCCCAAAATCAGAGAAGAATGTGAATTCCAAGAAAGGGATGT GTGTACAAAGGACAGACAATGCCAGGACAACAAGAAGTGTTGTGTCTTCAGCTGCGGAAAAAAATGTTTA GATCTCAAACAAGATGTATGCGAAATGCCAAAAGAAACTGGCCCCTGCCTGGCTTATTTTCTTCATTGGT GGTATGACAAGAAAGATAATACTTGCTCCATGTTTGTCTATGGTGGCTGCCAGGGAAACAATAACAACTT CCAATCCAAAGCCAACTGCCTGAACACCTGCAAGAATAAACGCTTTCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG206458 representing NM_020398
Red=Cloning site Green=Tags(s) MGSSGLLSLLVLFVLLANVQGPGLTDWLFPRRCPKIREECEFQERDVCTKDRQCQDNKKCCVFSCGKKCL DLKQDVCEMPKETGPCLAYFLHWWYDKKDNTCSMFVYGGCQGNNNNFQSKANCLNTCKNKRFP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020398 |
| ORF Size | 399 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020398.4 |
| RefSeq Size | 1961 bp |
| RefSeq ORF | 402 bp |
| Locus ID | 57119 |
| UniProt ID | O95925 |
| Protein Families | Secreted Protein |
| Gene Summary | This gene encodes an epididymal protease inhibitor, which contains both kunitz-type and WAP-type four-disulfide core (WFDC) protease inhibitor consensus sequences. Most WFDC genes are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene is a member of the WFDC gene family and belongs to the telomeric cluster. The protein can inhibit human sperm motility and exhibits antimicrobial activity against E. coli, and polymorphisms in this gene are associated with male infertility. Read-through transcription also exists between this gene and the downstream WFDC6 (WAP four-disulfide core domain 6) gene. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206458 | EPPIN (Myc-DDK-tagged)-Human serine peptidase inhibitor-like, with Kunitz and WAP domains 1 (eppin) (SPINLW1) |
CNY 1,200.00 |
|
| RC206458L3 | Lenti ORF clone of Human serine peptidase inhibitor-like, with Kunitz and WAP domains 1 (eppin) (SPINLW1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC206458L4 | Lenti ORF clone of Human serine peptidase inhibitor-like, with Kunitz and WAP domains 1 (eppin) (SPINLW1), mGFP tagged |
CNY 5,890.00 |
|
| SC126420 | EPPIN (untagged)-Human serine peptidase inhibitor-like, with Kunitz and WAP domains 1 (eppin) (SPINLW1) |
CNY 1,200.00 |
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