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Lipin 1 (LPIN1) (NM_145693) Human Tagged ORF Clone
CAT＃: RG207138
- TrueORF®
LPIN1 (tGFP-tagged) - Human lipin 1 (LPIN1)
ORF Plasmid: DDK
"NM_145693" in other vectors (6)
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CNY 8,120.00
| Cited in 1 publication. |
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | PAP1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG207138 representing NM_145693
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATTACGTGGGGCAGTTAGCCGGCCAGGTGTTTGTCACCGTGAAGGAGCTCTACAAGGGGCTGAATC CCGCCACACTCTCAGGGTGCATTGACATCATTGTCATCCGCCAGCCCAATGGAAACCTCCAATGCTCCCC TTTCCACGTCCGCTTTGGGAAGATGGGGGTCCTGCGCTCCCGAGAGAAAGTGGTTGACATAGAAATCAAT GGGGAATCTGTGGATTTGCATATGAAATTGGGAGATAATGGAGAAGCATTTTTTGTTCAAGAAACAGATA ATGATCAGGAAGTTATCCCTATGCACCTGGCCACCTCCCCCATCCTGTCAGAAGGAGCTTCGAGAATGGA ATGCCAGCTGAAAAGGGGCTCTGTGGACAGGATGAGAGGCCTGGACCCCAGCACGCCAGCCCAAGTGATC GCTCCCAGCGAGACGCCGTCAAGCAGCTCTGTAGTAAAGAAGAGAAGAAAAAGGAGGAGAAAGTCACAGC TGGACAGCCTGAAGAGAGATGACAACATGAACACATCTGAGGATGAGGACATGTTCCCCATCGAGATGAG CTCGGATGAGGCCATGGAGCTGCTGGAGAGCAGCAGAACTCTTCCTAATGATATACCTCCATTCCAAGAT GATATTCCTGAGGAAAACCTCTCCCTGGCTGTGATTTACCCTCAGTCAGCCTCATACCCTAATTCGGATA GAGAGTGGTCACCCACTCCCAGTCCTTCCGGTTCCCGACCTTCAACACCTAAAAGTGATTCAGAATTGGT CAGCAAGTCCACGGAAAGGACAGGGCAGAAGAACCCAGAAATGCTTTGGCTGTGGGGAGAGCTGCCGCAG GCTGCTAAGTCTTCTTCTCCACACAAGATGAAAGAGTCCAGCCCATTGAGCAGTAGAAAAATTTGTGATA AAAGTCACTTTCAGGCCATTCACAGCGAATCTTCAGACACTTTTAGTGACCAATCGCCAACTCTGGTCGG TGGGGCACTTTTGGACCAGAACAAGCCTCAGACAGAAATGCAGTTTGTGAATGAAGAAGACCTGGAGACC TTAGGAGCAGCAGCGCCACTCTTGCCCATGATCGAGGAGCTCAAACCCCCCTCTGCCAGTGTAGTCCAGA CAGCAAACAAGACGGATTCTCCTTCCAGGAAAAGAGATAAACGAAGCCGACATCTTGGTGCTGACGGCGT CTACTTGGATGACCTCACAGACATGGATCCTGAAGTGGCGGCCCTGTATTTTCCCAAAAACGGAGATCCT TCCGGACTCGCAAAACATGCAAGCGACAACGGAGCCCGGTCAGCCAACCAGTCCCCGCAGTCGGTGGGCA GCTCGGGCGTGGACAGTGGCGTGGAGAGCACCTCGGACGGGCTGAGGGACCTCCCTTCCATCGCCATCTC CCTCTGCGGGGGCCTCAGCGACCACCGGGAGATCACGAAAGATGCATTCCTGGAGCAAGCTGTGTCATAT CAACAGTTTGTGGACAACCCCGCTATTATCGATGACCCCAATCTCGTGGTAAAGATTGGGAGTAAATATT ATAACTGGACAACAGCAGCACCCCTCCTCCTGGCAATGCAGGCCTTCCAGAAACCTTTGCCAAAGGCCAC TGTGGAATCTATCATGAGGGATAAAATGCCCAAAAAGGGAGGAAGATGGTGGTTTTCATGGAGGGGAAGA AACACCACAATCAAGGAGGAAAGTAAGCCAGAGCAGTGCTTGGCTGGCAAGGCCCATAGCACCGGAGAGC AACCGCCGCAGCTCAGCTTGGCCACCAGGGTAAAGCATGAATCATCCTCCAGTGATGAGGAGCGCGCAGC TGCCAAGCCATCAAACGCAGGCCACCTCCCTCTTCTGCCTAATGTCAGCTACAAGAAGACTCTCCGGCTG ACTTCCGAGCAGCTTAAAAGCTTGAAGTTGAAGAATGGCCCCAACGACGTGGTTTTCAGTGTCACCACGC AGTACCAAGGCACGTGCCGCTGTGAGGGCACCATCTATCTGTGGAACTGGGATGATAAAGTCATCATTTC TGATATTGATGGGACAATTACCAGATCAGATACTCTTGGCCACATTTTGCCCACCCTTGGGAAGGATTGG ACCCATCAGGGCATCGCTAAGCTGTACCATAAAGTGAGCCAGAATGGATATAAATTTCTCTACTGTTCTG CCCGTGCCATCGGGATGGCGGACATGACGCGGGGCTACCTGCACTGGGTCAACGAGAGGGGCACGGTGCT GCCCCAGGGGCCCCTGCTGCTGAGTCCCAGCAGCCTCTTCTCTGCCCTGCACAGAGAAGTGATTGAAAAG AAGCCAGAAAAGTTTAAAGTCCAGTGTTTGACAGACATCAAAAACCTGTTTTTCCCCAACACAGAACCCT TTTATGCTGCTTTTGGAAACCGACCAGCTGATGTGTATTCATACAAGCAAGTAGGAGTGTCTTTGAATAG AATATTTACCGTCAACCCTAAAGGAGAGCTGGTACAGGAACATGCAAAGACCAACATCTCTTCGTATGTG AGACTCTGTGAAGTAGTCGACCACGTTTTCCCGTTGCTGAAAAGAAGCCATTCTTCAGACTTTCCCTGTT CGGATACCTTCAGTAACTTCACCTTTTGGAGAGAGCCACTGCCACCTTTTGAAAACCAGGACATTCATTC TGCCTCAGCG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG207138 representing NM_145693
Red=Cloning site Green=Tags(s) MNYVGQLAGQVFVTVKELYKGLNPATLSGCIDIIVIRQPNGNLQCSPFHVRFGKMGVLRSREKVVDIEIN GESVDLHMKLGDNGEAFFVQETDNDQEVIPMHLATSPILSEGASRMECQLKRGSVDRMRGLDPSTPAQVI APSETPSSSSVVKKRRKRRRKSQLDSLKRDDNMNTSEDEDMFPIEMSSDEAMELLESSRTLPNDIPPFQD DIPEENLSLAVIYPQSASYPNSDREWSPTPSPSGSRPSTPKSDSELVSKSTERTGQKNPEMLWLWGELPQ AAKSSSPHKMKESSPLSSRKICDKSHFQAIHSESSDTFSDQSPTLVGGALLDQNKPQTEMQFVNEEDLET LGAAAPLLPMIEELKPPSASVVQTANKTDSPSRKRDKRSRHLGADGVYLDDLTDMDPEVAALYFPKNGDP SGLAKHASDNGARSANQSPQSVGSSGVDSGVESTSDGLRDLPSIAISLCGGLSDHREITKDAFLEQAVSY QQFVDNPAIIDDPNLVVKIGSKYYNWTTAAPLLLAMQAFQKPLPKATVESIMRDKMPKKGGRWWFSWRGR NTTIKEESKPEQCLAGKAHSTGEQPPQLSLATRVKHESSSSDEERAAAKPSNAGHLPLLPNVSYKKTLRL TSEQLKSLKLKNGPNDVVFSVTTQYQGTCRCEGTIYLWNWDDKVIISDIDGTITRSDTLGHILPTLGKDW THQGIAKLYHKVSQNGYKFLYCSARAIGMADMTRGYLHWVNERGTVLPQGPLLLSPSSLFSALHREVIEK KPEKFKVQCLTDIKNLFFPNTEPFYAAFGNRPADVYSYKQVGVSLNRIFTVNPKGELVQEHAKTNISSYV RLCEVVDHVFPLLKRSHSSDFPCSDTFSNFTFWREPLPPFENQDIHSASA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_145693 |
| ORF Size | 2670 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_145693.4 |
| RefSeq Size | 5363 bp |
| RefSeq ORF | 2673 bp |
| Locus ID | 23175 |
| UniProt ID | Q14693 |
| Domains | lipin_N |
| Gene Summary | This gene encodes a magnesium-ion-dependent phosphatidic acid phosphohydrolase enzyme that catalyzes the penultimate step in triglyceride synthesis including the dephosphorylation of phosphatidic acid to yield diacylglycerol. Expression of this gene is required for adipocyte differentiation and it also functions as a nuclear transcriptional coactivator with some peroxisome proliferator-activated receptors to modulate expression of other genes involved in lipid metabolism. Mutations in this gene are associated with metabolic syndrome, type 2 diabetes, acute recurrent rhabdomyolysis, and autosomal recessive acute recurrent myoglobinuria (ARARM). This gene is also a candidate for several human lipodystrophy syndromes. [provided by RefSeq, Mar 2017] |
Citations (1)
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207138 | LPIN1 (Myc-DDK-tagged)-Human lipin 1 (LPIN1) |
CNY 6,520.00 |
|
| RC207138L1 | Lenti-ORF clone of LPIN1 (Myc-DDK-tagged)-Human lipin 1 (LPIN1) |
CNY 8,920.00 |
|
| RC207138L2 | Lenti-ORF clone of LPIN1 (mGFP-tagged)-Human lipin 1 (LPIN1) |
CNY 5,890.00 |
|
| RC207138L3 | Lenti-ORF clone of LPIN1 (Myc-DDK-tagged)-Human lipin 1 (LPIN1) |
CNY 8,920.00 |
|
| RC207138L4 | Lenti-ORF clone of LPIN1 (mGFP-tagged)-Human lipin 1 (LPIN1) |
CNY 5,890.00 |
|
| SC125492 | LPIN1 (untagged)-Human lipin 1 (LPIN1) |
CNY 6,528.00 |
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