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PTK9 (TWF1) (NM_002822) Human Tagged ORF Clone
CAT＃: RG207215
- TrueORF®
TWF1 (tGFP-tagged) - Human twinfilin, actin-binding protein, homolog 1 (Drosophila) (TWF1)
ORF Plasmid: DDK
"NM_002822" in other vectors (8)
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CNY 4,370.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | A6; PTK9 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG207215 representing NM_002822
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGCGCCGGGGCCGGCGGAGGAGCAGCCACTTCCTGGGGCCGCCGGCCGGGGCCGCTGGCTGCACTC AGCGCCGGAGCCGGGAGCTAGCGGCCGCCGCCATGTCCCACCAGACCGGCATCCAAGCAAGTGAAGATGT TAAAGAGATCTTTGCCAGAGCCAGAAATGGAAAGTACAGACTTCTGAAAATATCTATTGAAAATGAGCAA CTTGTGATTGGATCATATAGTCAGCCTTCAGATTCCTGGGATAAGGATTATGATTCCTTTGTTTTACCCC TGTTGGAGGACAAACAACCATGCTATATATTATTCAGGTTAGATTCTCAGAATGCCCAGGGATATGAATG GATATTCATTGCATGGTCTCCAGATCATTCTCATGTTCGTCAAAAAATGTTGTATGCAGCAACAAGAGCA ACTCTGAAGAAGGAATTTGGAGGTGGCCACATTAAAGATGAAGTATTTGGAACAGTAAAGGAAGATGTAT CATTACATGGATATAAAAAATACTTGCTGTCACAATCTTCCCCTGCCCCACTGACTGCAGCTGAGGAAGA ACTACGACAGATTAAAATCAATGAGGTACAGACTGACGTGGGTGTGGACACTAAGCATCAAACACTACAA GGAGTAGCATTTCCCATTTCTCGAGAAGCCTTTCAGGCTTTGGAAAAATTGAATAATAGACAGCTCAACT ATGTGCAGTTGGAAATAGATATAAAAAATGAAATTATAATTTTGGCCAACACAACAAATACAGAACTGAA AGATTTGCCAAAGAGGATTCCCAAGGATTCAGCTCGTTACCATTTCTTTCTGTATAAACATTCCCATGAA GGAGACTATTTAGAGTCCATAGTTTTTATTTATTCAATGCCTGGATACACATGCAGTATAAGAGAGCGGA TGCTGTATTCTAGCTGCAAGAGCCGTCTGCTAGAAATTGTAGAAAGACAACTACAAATGGATGTAATTAG AAAGATCGAGATAGACAATGGGGATGAGTTGACTGCAGACTTCCTTTATGAAGAAGTACATCCCAAGCAG CATGCACACAAGCAAAGTTTTGCAAAACCAAAAGGTCCTGCAGGAAAAAGAGGAATTCGAAGACTAATTA GGGGCCCAGCGGAAACTGAAGCTACTACTGAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG207215 representing NM_002822
Red=Cloning site Green=Tags(s) MTRRGRRRSSHFLGPPAGAAGCTQRRSRELAAAAMSHQTGIQASEDVKEIFARARNGKYRLLKISIENEQ LVIGSYSQPSDSWDKDYDSFVLPLLEDKQPCYILFRLDSQNAQGYEWIFIAWSPDHSHVRQKMLYAATRA TLKKEFGGGHIKDEVFGTVKEDVSLHGYKKYLLSQSSPAPLTAAEEELRQIKINEVQTDVGVDTKHQTLQ GVAFPISREAFQALEKLNNRQLNYVQLEIDIKNEIIILANTTNTELKDLPKRIPKDSARYHFFLYKHSHE GDYLESIVFIYSMPGYTCSIRERMLYSSCKSRLLEIVERQLQMDVIRKIEIDNGDELTADFLYEEVHPKQ HAHKQSFAKPKGPAGKRGIRRLIRGPAETEATTD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002822 |
| ORF Size | 1152 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002822.3, NP_002813.2 |
| RefSeq Size | 3065 bp |
| RefSeq ORF | 1053 bp |
| Locus ID | 5756 |
| UniProt ID | Q12792 |
| Domains | ADF |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207215 | TWF1 (Myc-DDK-tagged)-Human twinfilin, actin-binding protein, homolog 1 (Drosophila) (TWF1) |
CNY 3,656.00 |
|
| RC207215L1 | Lenti ORF clone of Human twinfilin, actin-binding protein, homolog 1 (Drosophila) (TWF1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207215L2 | Lenti ORF clone of Human twinfilin, actin-binding protein, homolog 1 (Drosophila) (TWF1), mGFP tagged |
CNY 5,890.00 |
|
| RC207215L3 | Lenti ORF clone of Human twinfilin, actin-binding protein, homolog 1 (Drosophila) (TWF1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207215L4 | Lenti ORF clone of Human twinfilin, actin-binding protein, homolog 1 (Drosophila) (TWF1), mGFP tagged |
CNY 5,890.00 |
|
| SC109662 | TWF1 (untagged)-Human twinfilin, actin-binding protein, homolog 1 (Drosophila) (TWF1) |
CNY 2,950.00 |
|
| SC317613 | TWF1 (untagged)-Human twinfilin, actin-binding protein, homolog 1 (Drosophila) (TWF1) |
CNY 3,656.00 |
|
| SC317614 | TWF1 (untagged)-Human twinfilin, actin-binding protein, homolog 1 (Drosophila) (TWF1) |
CNY 7,220.00 |
