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Lebercilin (LCA5) (NM_181714) Human Tagged ORF Clone
CAT＃: RG207993
- TrueORF®
LCA5 (tGFP-tagged) - Human Leber congenital amaurosis 5 (LCA5), transcript variant 1
ORF Plasmid: DDK
"NM_181714" in other vectors (4)
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CNY 4,370.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C6orf152 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG207993 representing NM_181714
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGAAAGAGCAGGAAGTCCAGGTACTGATCAAGAAAGAGAGGCAGGCAAACACCATTATTCTTACT CATCTGATTTTGAAACGCCACAGTCTTCTGGCCGATCATCGCTGGTCAGTTCTTCACCTGCAAGTGTTAG GAGAAAAAATCCTAAAAGACAAACTTCAGATGGCCAAGTACATCACCAAGCCCCTCGGAAACCAAGCCCT AAGGGTCTACCAAACAGAAAGGGAGTCCGAGTGGGATTTCGCTCCCAGAGCCTCAATAGAGAGCCACTTC GGAAAGATACTGATCTTGTTACAAAACGGATTCTGTCTGCAAGACTGCTAAAAATCAATGAGTTGCAGAA TGAAGTATCTGAACTCCAGGTCAAGTTAGCTGAGCTGCTAAAAGAAAATAAATCTTTGAAAAGGCTTCAG TACAGACAGGAGAAAGCCCTGAATAAGTTTGAAGATGCCGAAAATGAAATCTCACAACTTATATTTCGTC ATAACAATGAGATTACAGCACTCAAAGAACGCTTAAGAAAATCTCAAGAGAAAGAACGGGCAACTGAGAA AAGGGTAAAAGATACAGAAAGTGAACTATTTAGGACAAAATTTTCCTTACAGAAACTGAAAGAGATCTCT GAAGCTAGACACCTACCTGAACGAGATGATTTGGCAAAGAAACTAGTTTCAGCAGAGTTAAAGTTAGATG ACACCGAGAGAAGAATTAAGGAGCTATCGAAAAACCTTGAACTGAGTACTAACAGTTTCCAACGACAGTT GCTTGCTGAAAGGAAAAGGGCATATGAGGCTCATGATGAAAATAAAGTTCTTCAAAAGGAGGTACAGCGA CTATATCACAAATTAAAGGAAAAGGAGAGAGAACTGGATATAAAAAATATATATTCTAATCGTCTGCCAA AGTCCTCTCCAAATAAAGAGAAAGAACTTGCATTAAGAAAAAATGCTGCATGCCAGAGTGATTTTGCAGA CCTGTGTACAAAAGGAGTACAAACCATGGAAGACTTCAAGCCAGAAGAATATCCTTTAACTCCAGAAACA ATTATGTGTTACGAAAACAAATGGGAAGAACCAGGACATCTTACTTTGGACTTGCAATCTCAAAAGCAAG ACAGGCATGGAGAAGCAGGGATTCTAAACCCAATTATGGAAAGAGAAGAAAAATTTGTTACAGATGAAGA ACTCCATGTCGTAAAACAGGAGGTTGAAAAGCTGGAGGATGAATGGGAAAGAGAAGAACTTGATAAAAAG CAAAAAGAAAAGGCATCTTTACTGGAAAGAGAAGAAAAGCCAGAGTGGGAAACTGGAAGGTACCAACTAG GAATGTATCCAATTCAGAATATGGATAAATTGCAAGGAGAGGAAGAAGAAAGACTGAAGAGAGAAATGCT ACTTGCTAAACTGAATGAAATTGACAGAGAACTCCAAGATTCTCGAAATCTAAAATACCCTGTTTTGCCA TTGTTACCTGATTTTGAATCAAAACTACACTCCCGAGAGAGAAGCCCCAAAACATACAGGTTCTCTGAAT CCTCAGAGAGATTATTTAATGGGCATCATTTGCAAGACATCAGTTTCTCAACTCCAAAAGGAGAAGGTCA GAATTCAGGAAATGTTAGAAGTCCAGCCTCCCCTAATGAGTTCGCATTTGGTAGCTACGTGCCTTCGTTT GCAAAAACATCAGAGAGGTCAAATCCATTTAGTCAAAAAAGTAGTTTTTTGGATTTCCAAAGAAACAGTA TGGAAAAACTTAGTAAAGATGGTGTAGATTTAATTACAAGAAAAGAGAAAAAAGCTAATTTGATGGAACA GTTATTTGGTGCCAGTGGTAGCAGCACCATTTCCTCCAAAAGCAGTGGCCCAAATTCTGTGGCTTCCAGT AAAGGAGACATTGACCCTCTAAATTTTCTCCCTGGGAATAAAGGCAGCAGAGATCAAGAACATGATGAAG ATGAAGGCTTTTTCCTCAGTGAAGGAAGAAGTTTTAATCCAAATAGGCACCGATTAAAACATGCAGACGA TAAACCAGCAGTAAAAGCAGCTGATTCTGTAGAAGATGAAATTGAAGAAGTAGCACTGAGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG207993 representing NM_181714
Red=Cloning site Green=Tags(s) MGERAGSPGTDQEREAGKHHYSYSSDFETPQSSGRSSLVSSSPASVRRKNPKRQTSDGQVHHQAPRKPSP KGLPNRKGVRVGFRSQSLNREPLRKDTDLVTKRILSARLLKINELQNEVSELQVKLAELLKENKSLKRLQ YRQEKALNKFEDAENEISQLIFRHNNEITALKERLRKSQEKERATEKRVKDTESELFRTKFSLQKLKEIS EARHLPERDDLAKKLVSAELKLDDTERRIKELSKNLELSTNSFQRQLLAERKRAYEAHDENKVLQKEVQR LYHKLKEKERELDIKNIYSNRLPKSSPNKEKELALRKNAACQSDFADLCTKGVQTMEDFKPEEYPLTPET IMCYENKWEEPGHLTLDLQSQKQDRHGEAGILNPIMEREEKFVTDEELHVVKQEVEKLEDEWEREELDKK QKEKASLLEREEKPEWETGRYQLGMYPIQNMDKLQGEEEERLKREMLLAKLNEIDRELQDSRNLKYPVLP LLPDFESKLHSRERSPKTYRFSESSERLFNGHHLQDISFSTPKGEGQNSGNVRSPASPNEFAFGSYVPSF AKTSERSNPFSQKSSFLDFQRNSMEKLSKDGVDLITRKEKKANLMEQLFGASGSSTISSKSSGPNSVASS KGDIDPLNFLPGNKGSRDQEHDEDEGFFLSEGRSFNPNRHRLKHADDKPAVKAADSVEDEIEEVALR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_181714 |
| ORF Size | 2091 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_181714.1, NP_859065.1 |
| RefSeq Size | 4733 bp |
| RefSeq ORF | 2094 bp |
| Locus ID | 167691 |
| UniProt ID | Q86VQ0 |
| Gene Summary | This gene encodes a protein that is thought to be involved in centrosomal or ciliary functions. Mutations in this gene cause Leber congenital amaurosis type V. Alternatively spliced transcript variants are described. [provided by RefSeq, Oct 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207993 | LCA5 (Myc-DDK-tagged)-Human Leber congenital amaurosis 5 (LCA5), transcript variant 1 |
CNY 3,990.00 |
|
| RC207993L3 | Lenti ORF clone of Human Leber congenital amaurosis 5 (LCA5), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207993L4 | Lenti ORF clone of Human Leber congenital amaurosis 5 (LCA5), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| SC107321 | LCA5 (untagged)-Human Leber congenital amaurosis 5 (LCA5), transcript variant 1 |
CNY 2,950.00 |
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