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TRM1 (TRMT1) (NM_017722) Human Tagged ORF Clone
CAT＃: RG208040
- TrueORF®
TRMT1 (tGFP-tagged) - Human TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae) (TRMT1), transcript variant 1
ORF Plasmid: DDK
"NM_017722" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MRT68; TRM1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG208040 representing NM_017722
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAAGGATCGTCTCTGTGGCTAAGCCTCACTTTCCGCTCCGCCCGGGTGCTCTCTAGAGCCCGGTTTT TCGAGTGGCAGTCTCCAGGGCTGCCGAATACAGCAGCGATGGAGAACGGCACCGGGCCCTACGGAGAAGA ACGTCCACGTGAAGTCCAGGAGACGACAGTCACCGAGGGGGCTGCCAAAATCGCCTTTCCCAGTGCCAAC GAGGTCTTTTATAACCCGGTGCAGGAATTCAATCGGGACCTGACATGTGCTGTGATCACCGAGTTTGCTC GCATTCAGCTTGGGGCCAAAGGAATCCAGATCAAGGTTCCAGGAGAGAAGGACACGCAAAAAGTGGTCGT GGACTTGTCAGAGCAAGAGGAGGAAAAGGTTGAACTGAAAGAGAGTGAAAACCTGGCCTCAGGAGACCAA CCTCGCACAGCGGCCGTGGGGGAGATCTGTGAGGAAGGCCTGCATGTGCTGGAAGGCCTGGCAGCTTCAG GCCTACGTTCCATTCGATTTGCCCTAGAGGTGCCTGGGCTCAGATCTGTGGTTGCAAACGATGCCTCCAC CCGGGCTGTGGATCTCATACGCCGGAATGTCCAGCTCAATGACGTGGCCCACCTGGTACAGCCGAGCCAA GCAGATGCCCGGATGCTGATGTACCAGCACCAGAGGGTGTCGGAGAGGTTTGACGTCATCGATCTGGACC CCTATGGCAGCCCAGCCACCTTCCTGGATGCAGCTGTGCAGGCTGTGAGTGAAGGAGGGTTGCTGTGTGT GACCTGCACAGACATGGCGGTGTTGGCGGGGAACAGCGGGGAGACGTGCTACAGCAAGTACGGGGCCATG GCCCTCAAGAGCCGGGCCTGCCACGAGATGGCCCTGAGAATCGTCCTGCACAGCCTGGACCTCCGCGCCA ACTGCTACCAGCGCTTCGTGGTGCCGCTGCTCAGCATCAGCGCTGACTTCTACGTGCGTGTTTTTGTCCG TGTCTTCACCGGCCAGGCCAAGGTCAAGGCCTCAGCCAGCAAGCAGGCGCTGGTGTTCCAGTGTGTGGGC TGCGGGGCCTTCCACCTTCAGCGTCTCGGCAAAGCGTCAGGAGTCCCCAGCGGCCGGGCCAAGTTCTCTG CAGCCTGTGGTCCCCCTGTGACCCCCGAGTGTGAACACTGTGGGCAACGACACCAGCTTGGTGGCCCCAT GTGGGCAGAGCCCATCCATGACCTGGATTTTGTGGGCCGTGTCCTGGAGGCTGTGAGCGCTAACCCCGGC CGCTTCCACACCTCGGAGCGGATCCGAGGGGTCCTGAGCGTCATCACTGAGGAGCTCCCGGACGTGCCTC TGTACTACACCCTGGACCAGCTGAGCAGCACCATCCACTGCAACACACCAAGCCTCCTGCAGTTGCGGTC GGCCCTCCTCCACGCTGACTTCCGGGTCTCACTCTCCCACGCCTGTAAGAACGCTGTGAAGACGGATGCC CCTGCCTCTGCCCTCTGGGACATCATGCGTTGCTGGGAGAAGGAATGTCCGGTGAAACGGGAGCGACTAT CAGAGACTAGCCCAGCGTTCCGCATTCTCAGTGTGGAGCCCAGGCTGCAGGCCAACTTCACCATCCGGGA AGATGCCAACCCCAGCTCCCGACAGCGAGGACTCAAGCGCTTCCAGGCTAACCCGGAGGCCAACTGGGGT CCCCGGCCTCGTGCCCGGCCAGGGGGCAAGGCGGCCGACGAAGCTATGGAGGAGAGACGCAGGCTGCTTC AGAACAAGCGGAAGGAGCCGCCGGAAGATGTGGCCCAGCGGGCTGCCCGGCTCAAGACATTTCCTTGCAA GAGGTTTAAGGAGGGCACCTGTCAACGCGGGGACCAGTGCTGCTACTCCCACAGCCCCCCGACACCCAGG GTTTCTGCTGATGCTGCCCCTGACTGTCCAGAGACCTCCAACCAGACCCCCCCTGGACCTGGGGCTGCCG CTGGGCCAGGCATAGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG208040 representing NM_017722
Red=Cloning site Green=Tags(s) MQGSSLWLSLTFRSARVLSRARFFEWQSPGLPNTAAMENGTGPYGEERPREVQETTVTEGAAKIAFPSAN EVFYNPVQEFNRDLTCAVITEFARIQLGAKGIQIKVPGEKDTQKVVVDLSEQEEEKVELKESENLASGDQ PRTAAVGEICEEGLHVLEGLAASGLRSIRFALEVPGLRSVVANDASTRAVDLIRRNVQLNDVAHLVQPSQ ADARMLMYQHQRVSERFDVIDLDPYGSPATFLDAAVQAVSEGGLLCVTCTDMAVLAGNSGETCYSKYGAM ALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFYVRVFVRVFTGQAKVKASASKQALVFQCVG CGAFHLQRLGKASGVPSGRAKFSAACGPPVTPECEHCGQRHQLGGPMWAEPIHDLDFVGRVLEAVSANPG RFHTSERIRGVLSVITEELPDVPLYYTLDQLSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDA PASALWDIMRCWEKECPVKRERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWG PRPRARPGGKAADEAMEERRRLLQNKRKEPPEDVAQRAARLKTFPCKRFKEGTCQRGDQCCYSHSPPTPR VSADAAPDCPETSNQTPPGPGAAAGPGID TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017722 |
| ORF Size | 1977 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017722.4 |
| RefSeq Size | 2217 bp |
| RefSeq ORF | 1980 bp |
| Locus ID | 55621 |
| UniProt ID | Q9NXH9 |
| Domains | zf-CCCH, TRM |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a tRNA-modifying enzyme that acts as a dimethyltransferase, modifying a single guanine residue at position 26 of the tRNA. The encoded enzyme has both mono- and dimethylase activity when exogenously expressed, and uses S-adenosyl methionine as a methyl donor. The C-terminal region of the encoded protein has both a zinc finger motif, and an arginine/proline-rich region. Mutations in this gene have been implicated in autosomal recessive intellectual disorder (ARID). Alternative splicing results in multiple transcript variants encoding different isoforms. There is a pseudogene of this gene on the X chromosome. [provided by RefSeq, May 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208040 | TRMT1 (Myc-DDK-tagged)-Human TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae) (TRMT1), transcript variant 1 |
CNY 7,360.00 |
|
| RC208040L3 | Lenti-ORF clone of TRMT1 (Myc-DDK-tagged)-Human TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae) (TRMT1), transcript variant 1 |
CNY 5,890.00 |
|
| RC208040L4 | Lenti-ORF clone of TRMT1 (mGFP-tagged)-Human TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae) (TRMT1), transcript variant 1 |
CNY 5,890.00 |
|
| SC114002 | TRMT1 (untagged)-Human TRM1 tRNA methyltransferase 1 homolog (S. cerevisiae) (TRMT1), transcript variant 1 |
CNY 4,912.00 |
