GNAL (NM_182978) Human Tagged ORF Clone
CAT#: RG208226
- TrueORF®
GNAL (tGFP-tagged) - Human guanine nucleotide binding protein (G protein), alpha activating activity polypeptide, olfactory type (GNAL), transcript variant 1
ORF Plasmid: DDK
"NM_182978" in other vectors (4)
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CNY 7,088.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | DYT25 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG208226 representing NM_182978
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTCTGTGCTACAGTCTGCGGCCGCTGCTTTTCGGGGGCCCAGGGGACGACCCCTGCGCGGCCTCGG AGCCGCCGGTGGAGGACGCGCAGCCCGCCCCGGCCCCGGCCCTGGCCCCAGTCCGGGCGGCCGCAAGGGA CACGGCCCGGACCCTGCTCCCTCGGGGCGGCGAAGGGAGCCCGGCATGCGCTCGGCCCAAAGCAGACAAG CCGAAGGAGAAGCGGCAGCGCACCGAGCAGCTTAGTGCCGAGGAGCGCGAGGCGGCCAAGGAGCGCGAGG CGGTCAAGGAGGCGAGGAAAGTGAGCCGGGGCATCGACCGCATGCTGCGCGACCAGAAGCGCGACCTGCA GCAGACGCACCGGCTCCTGCTGCTCGGGGCTGGTGAGTCTGGGAAAAGCACTATCGTCAAACAGATGAGG ATCCTGCACGTCAATGGGTTTAATCCCGAGGAAAAGAAACAGAAAATTCTGGACATCCGGAAAAATGTTA AAGATGCTATCGTGACAATTGTTTCAGCAATGAGTACTATAATACCTCCAGTTCCGCTGGCCAACCCTGA AAACCAATTTCGATCAGACTACATCAAGAGCATAGCCCCTATCACTGACTTTGAATATTCCCAGGAATTC TTTGACCATGTGAAAAAACTTTGGGACGATGAAGGCGTGAAGGCATGCTTTGAGAGATCCAACGAATACC AGCTGATTGACTGTGCACAATACTTCCTGGAAAGAATCGACAGCGTCAGCTTGGTTGACTACACACCCAC AGACCAGGACCTCCTCAGATGCAGAGTTCTGACATCTGGGATTTTTGAGACACGATTCCAAGTGGACAAA GTAAACTTCCACATGTTTGATGTTGGTGGCCAGAGGGATGAGAGGAGAAAATGGATCCAGTGCTTTAACG ATGTCACAGCTATCATTTACGTCGCAGCCTGCAGTAGCTACAACATGGTGATTCGAGAAGATAACAACAC CAACAGGCTGAGAGAGTCCCTGGATCTTTTTGAAAGCATCTGGAACAACAGGTGGTTACGGACCATTTCT ATCATCTTGTTCTTGAACAAACAAGATATGCTGGCAGAAAAAGTCTTGGCAGGGAAATCAAAAATTGAAG ACTATTTCCCAGAATATGCAAATTATACTGTTCCTGAAGACGCAACACCAGATGCAGGAGAAGATCCCAA AGTTACAAGAGCCAAGTTCTTTATCCGGGACCTGTTTTTGAGGATCAGCACGGCCACCGGTGACGGCAAA CATTACTGCTACCCGCACTTCACCTGCGCCGTGGACACAGAGAACATCCGCAGGGTGTTCAACGACTGCC GCGACATCATCCAGCGGATGCACCTCAAGCAGTATGAGCTCTTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG208226 representing NM_182978
Red=Cloning site Green=Tags(s) MGLCYSLRPLLFGGPGDDPCAASEPPVEDAQPAPAPALAPVRAAARDTARTLLPRGGEGSPACARPKADK PKEKRQRTEQLSAEEREAAKEREAVKEARKVSRGIDRMLRDQKRDLQQTHRLLLLGAGESGKSTIVKQMR ILHVNGFNPEEKKQKILDIRKNVKDAIVTIVSAMSTIIPPVPLANPENQFRSDYIKSIAPITDFEYSQEF FDHVKKLWDDEGVKACFERSNEYQLIDCAQYFLERIDSVSLVDYTPTDQDLLRCRVLTSGIFETRFQVDK VNFHMFDVGGQRDERRKWIQCFNDVTAIIYVAACSSYNMVIREDNNTNRLRESLDLFESIWNNRWLRTIS IILFLNKQDMLAEKVLAGKSKIEDYFPEYANYTVPEDATPDAGEDPKVTRAKFFIRDLFLRISTATGDGK HYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLKQYELL TRTRPLE - GFP Tag - V |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_182978 |
ORF Size | 1374 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_182978.1, NP_892023.1 |
RefSeq Size | 6491 bp |
RefSeq ORF | 1377 bp |
Locus ID | 2774 |
UniProt ID | P38405 |
Protein Families | Druggable Genome |
Protein Pathways | Calcium signaling pathway, Olfactory transduction |
Gene Summary | This gene encodes a stimulatory G protein alpha subunit which mediates odorant signaling in the olfactory epithelium. This protein couples dopamine type 1 receptors and adenosine A2A receptors and is widely expressed in the central nervous system. Mutations in this gene have been associated with dystonia 25 and this gene is located in a susceptibility region for bipolar disorder and schizophrenia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC208226 | GNAL (Myc-DDK-tagged)-Human guanine nucleotide binding protein (G protein), alpha activating activity polypeptide, olfactory type (GNAL), transcript variant 1 |
CNY 5,488.00 |
|
RC208226L3 | Lenti-ORF clone of GNAL (Myc-DDK-tagged)-Human guanine nucleotide binding protein (G protein), alpha activating activity polypeptide, olfactory type (GNAL), transcript variant 1 |
CNY 5,890.00 |
|
RC208226L4 | Lenti-ORF clone of GNAL (mGFP-tagged)-Human guanine nucleotide binding protein (G protein), alpha activating activity polypeptide, olfactory type (GNAL), transcript variant 1 |
CNY 5,890.00 |
|
SC107605 | GNAL (untagged)-Human guanine nucleotide binding protein (G protein), alpha activating activity polypeptide, olfactory type (GNAL), transcript variant 1 |
CNY 3,656.00 |