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TSEN54 (NM_207346) Human Tagged ORF Clone
CAT＃: RG208425
- TrueORF®
TSEN54 (tGFP-tagged) - Human tRNA splicing endonuclease 54 homolog (S. cerevisiae) (TSEN54)
ORF Plasmid: DDK
"NM_207346" in other vectors (6)
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CNY 5,040.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | PCH2A; PCH4; PCH5; sen54; SEN54L |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG208425 representing NM_207346
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCCGATCCCGAGCCCGCGGCCGTGGAGGTTCCCGCGGGGCGCGTGCTCAGCGCCCGGGAGCTCT TCGCCGCCCGCTCGCGGTCGCAGAAGCTGCCCCAGCGCTCGCATGGCCCCAAGGACTTTCTGCCCGACGG CTCGGCAGCTCAGGCCGAGCGGCTGCGCCGGTGCCGGGAAGAGCTCTGGCAGCTGCTGGCAGAGCAGCGC GTGGAGCGCCTGGGCAGCTTGGTGGCTGCCGAGTGGAGGCCAGAAGAGGGCTTCGTGGAGTTGAAGTCTC CCGCGGGCAAATTCTGGCAGACCATGGGCTTCTCAGAGCAGGGCCGGCAGCGCCTTCACCCGGAAGAGGC CTTGTATCTTCTGGAGTGTGGCTCCATCCACCTCTTCCACCAAGACCTGCCACTGTCTATCCAGGAAGCT TACCAGCTGCTGCTGACCGACCACACTGTGACCTTCCTGCAGTACCAGGTCTTCAGCCACCTGAAGAGGT TGGGTTATGTGGTTCGACGATTCCAACCAAGCTCTGTCCTGTCCCCGTATGAGAGGCAGCTTAACCTGGA TGCCAGCGTGCAGCACTTGGAGGATGGAGATGGCAAGAGAAAGAGGAGCAGCTCCAGCCCTCGGTCCATT AATAAGAAGGCCAAGGCCCTGGACAACTCCCTGCAACCCAAGAGTCTGGCAGCCTCCAGCCCACCTCCCT GCAGCCAGCCCAGCCAATGCCCAGAGGAGAAACCCCAGGAGTCAAGCCCCATGAAGGGCCCAGGGGGCCC CTTTCAGCTTCTGGGGTCCCTGGGCCCCAGCCCTGGCCCGGCCAGGGAGGGGGTGGGGTGCAGCTGGGAG AGTGGCAGAGCCGAGAACGGAGTCACGGGAGCCGGTAAGCGGCGCTGGAACTTCGAGCAGATCTCCTTCC CCAACATGGCTTCAGACAGCCGCCACACCCTTCTGCGCGCCCCAGCCCCAGAGCTGCTCCCGGCCAACGT GGCTGGGCGGGAGACAGACGCTGAGTCCTGGTGCCAGAAGCTGAACCAGCGCAAGGAGAACCTCTCCAGG CGGGAACGGGAGCACCACGCGGAGGCCGCGCAGTTCCAGGAAGATGTCAACGCCGATCCCGAGGTGCAGC GCTGCTCCAGCTGGCGGGAGTACAAGGAGCTGCTGCAGCGGCGGCAGGTGCAGAGGAGCCAGCGCCGGGC CCCTCACCTGTGGGGCCAGCCCGTCACCCCGCTGCTGAGTCCTGGCCAGGCCAGCTCCCCAGCCGTGGTC CTTCAGCATATCTCTGTGCTGCAGACAACACACCTTCCTGATGGAGGTGTCCGGCTGTTGGAGAAGTCTG GGGGCTTGGAAATCATCTTTGATGTTTACCAGGCCGACGCTGTGGCCACATTCCGAAAGAATAACCCTGG CAAACCCTATGCCCGGATGTGCATTAGTGGATTTGATGAGCCTGTCCCAGACCTCTGCAGCCTCAAGCGG TTGTCTTACCAGAGTGGGGATGTCCCTCTGATCTTTGCCCTGGTGGATCATGGTGACATCTCCTTCTACA GCTTCAGGGACTTCACGTTGCCCCAGGATGTGGGGCAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG208425 representing NM_207346
Red=Cloning site Green=Tags(s) MEPDPEPAAVEVPAGRVLSARELFAARSRSQKLPQRSHGPKDFLPDGSAAQAERLRRCREELWQLLAEQR VERLGSLVAAEWRPEEGFVELKSPAGKFWQTMGFSEQGRQRLHPEEALYLLECGSIHLFHQDLPLSIQEA YQLLLTDHTVTFLQYQVFSHLKRLGYVVRRFQPSSVLSPYERQLNLDASVQHLEDGDGKRKRSSSSPRSI NKKAKALDNSLQPKSLAASSPPPCSQPSQCPEEKPQESSPMKGPGGPFQLLGSLGPSPGPAREGVGCSWE SGRAENGVTGAGKRRWNFEQISFPNMASDSRHTLLRAPAPELLPANVAGRETDAESWCQKLNQRKENLSR REREHHAEAAQFQEDVNADPEVQRCSSWREYKELLQRRQVQRSQRRAPHLWGQPVTPLLSPGQASSPAVV LQHISVLQTTHLPDGGVRLLEKSGGLEIIFDVYQADAVATFRKNNPGKPYARMCISGFDEPVPDLCSLKR LSYQSGDVPLIFALVDHGDISFYSFRDFTLPQDVGH TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_207346 |
| ORF Size | 1578 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_207346.1, NP_997229.1 |
| RefSeq Size | 1970 bp |
| RefSeq ORF | 1581 bp |
| Locus ID | 283989 |
| UniProt ID | Q7Z6J9 |
| Gene Summary | This gene encodes a subunit of the tRNA splicing endonuclease complex, which catalyzes the removal of introns from precursor tRNAs. The complex is also implicated in pre-mRNA 3-prime end processing. Mutations in this gene result in pontocerebellar hypoplasia type 2.[provided by RefSeq, Oct 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208425 | TSEN54 (Myc-DDK-tagged)-Human tRNA splicing endonuclease 54 homolog (S. cerevisiae) (TSEN54) |
CNY 3,920.00 |
|
| RC208425L1 | Lenti ORF clone of Human tRNA splicing endonuclease 54 homolog (S. cerevisiae) (TSEN54), Myc-DDK-tagged |
CNY 6,320.00 |
|
| RC208425L2 | Lenti ORF clone of Human tRNA splicing endonuclease 54 homolog (S. cerevisiae) (TSEN54), mGFP tagged |
CNY 6,460.00 |
|
| RC208425L3 | Lenti ORF clone of Human tRNA splicing endonuclease 54 homolog (S. cerevisiae) (TSEN54), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC208425L4 | Lenti ORF clone of Human tRNA splicing endonuclease 54 homolog (S. cerevisiae) (TSEN54), mGFP tagged |
CNY 6,460.00 |
|
| SC323994 | TSEN54 (untagged)-Human tRNA splicing endonuclease 54 homolog (S. cerevisiae) (TSEN54) |
CNY 3,928.00 |
