PLEKHA3 (NM_019091) Human Tagged ORF Clone

CAT#: RG208433

  • TrueORF®

PLEKHA3 (tGFP-tagged) - Human pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 3 (PLEKHA3)

ORF Plasmid: DDK tGFP



  "NM_019091" in other vectors (6)

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CNY 4,000.00

CNY 4,370.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


PLEKHA3 mouse monoclonal antibody, clone OTI5D11 (formerly 5D11)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms FAPP1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG208433 representing NM_019091
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGGGGTGTTGTACAAGTGGACCAACTATCTCACAGGCTGGCAGCCTCGTTGGTTTGTTTTAGATA
ATGGAATCTTATCCTACTATGATTCACAAGATGATGTTTGCAAAGGGAGCAAAGGAAGCATAAAGATGGC
AGTTTGTGAAATTAAAGTTCATTCAGCAGACAACACAAGAATGGAATTAATCATTCCTGGAGAGCAGCAT
TTCTACATGAAGGCAGTGAATGCAGCTGAAAGACAGAGGTGGCTGGTCGCTCTGGGGAGCTCCAAAGCAT
GTTTGACTGATACAAGGACTAAAAAAGAAAAAGAAATAAGTGAAACCAGTGAATCGCTGAAAACCAAAAT
GTCTGAACTTCGCCTCTACTGTGACCTCTTAATGCAGCAAGTTCATACAATACAGGAATTTGTTCACCAT
GATGAGAATCATTCATCTCCTAGTGCAGAGAACATGAATGAAGCCTCTTCTCTGCTTAGTGCCACGTGTA
ACACATTCATCACAACGCTTGAGGAATGTGTGAAGATAGCCAATGCCAAGTTTAAACCTGAGATGTTTCA
ACTGCACCATCCGGATCCCTTAGTTTCTCCTGTGTCACCTTCTCCTGTTCAAATGATGAAGCGTTCTGTC
AGCCACCCTGGTTCTTGCAGTTCAGAGAGGAGTAGCCACTCTATAAAAGAACCAGTATCTACACTTCACC
GACTCTCCCAGCGACGCCGAAGAACCTACTCAGATACAGATTCTTGTAGTGATATTCCTCTTGAAGACCC
AGATAGACCTGTTCACTGTTCAAAAAATACACTTAATGGAGATTTGGCATCAGCAACCATTCCTGAAGAA
AGCAGACTTATGGCCAAAAAACAATCTGAATCAGAAGATACTCTTCCATCCTTCTCTTCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG208433 representing NM_019091
Red=Cloning site Green=Tags(s)

MEGVLYKWTNYLTGWQPRWFVLDNGILSYYDSQDDVCKGSKGSIKMAVCEIKVHSADNTRMELIIPGEQH
FYMKAVNAAERQRWLVALGSSKACLTDTRTKKEKEISETSESLKTKMSELRLYCDLLMQQVHTIQEFVHH
DENHSSPSAENMNEASSLLSATCNTFITTLEECVKIANAKFKPEMFQLHHPDPLVSPVSPSPVQMMKRSV
SHPGSCSSERSSHSIKEPVSTLHRLSQRRRRTYSDTDSCSDIPLEDPDRPVHCSKNTLNGDLASATIPEE
SRLMAKKQSESEDTLPSFSS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_019091
ORF Size 900 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_019091.4
RefSeq Size 2516 bp
RefSeq ORF 903 bp
Locus ID 65977
UniProt ID Q9HB20
Domains PH
Gene Summary Involved in Golgi to cell surface membrane traffic. Induces membrane tubulation. Binds preferentially to phosphatidylinositol 4-phosphate (PtdIns4P).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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