Atrophin 1 (ATN1) (NM_001940) Human Tagged ORF Clone
CAT#: RG208519
- TrueORF®
ATN1 (tGFP-tagged) - Human atrophin 1 (ATN1), transcript variant 2
ORF Plasmid: DDK
"NM_001940" in other vectors (4)
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CNY 13,110.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | B37; CHEDDA; D12S755E; DRPLA; HRS; NOD |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG208519 representing NM_001940
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGACACGACAGAATAAAGACTCGATGTCAATGAGGAGTGGACGGAAGAAAGAGGCCCCTGGGCCCC GGGAAGAACTGAGATCGAGGGGCCGGGCCTCCCCTGGAGGGGTCAGCACGTCCAGCAGTGATGGCAAAGC TGAGAAGTCCAGGCAGACAGCCAAGAAGGCCCGAGTAGAGGAAGCCTCCACCCCAAAGGTCAACAAGCAG GGTCGGAGTGAGGAGATCTCAGAGAGTGAAAGTGAGGAGACCAATGCACCAAAAAAGACCAAAACTGAGC AGGAACTCCCTCGGCCACAGTCTCCCTCCGATCTGGATAGCTTGGACGGGCGGAGCCTTAATGATGATGG CAGCAGCGACCCTAGGGATATCGACCAGGACAACCGAAGCACGTCCCCCAGTATCTACAGCCCTGGAAGT GTGGAGAATGACTCTGACTCATCTTCTGGCCTGTCCCAGGGCCCAGCCCGCCCCTACCACCCACCTCCAC TCTTTCCTCCTTCCCCTCAACCGCCAGACAGCACCCCTCGACAGCCAGAGGCTAGCTTTGAACCCCATCC TTCTGTGACACCCACTGGATATCATGCTCCCATGGAGCCCCCCACATCTCGAATGTTCCAGGCTCCTCCT GGGGCCCCTCCCCCTCACCCACAGCTCTATCCTGGGGGCACTGGTGGAGTTTTGTCTGGACCCCCAATGG GTCCCAAGGGGGGAGGGGCTGCCTCATCAGTGGGGGGCCCTAATGGGGGTAAGCAGCACCCCCCACCCAC TACTCCCATTTCAGTATCAAGCTCTGGGGCTAGTGGTGCTCCCCCAACAAAGCCGCCTACCACTCCAGTG GGTGGTGGGAACCTACCTTCTGCTCCACCACCAGCCAACTTCCCCCATGTGACACCGAACCTGCCTCCCC CACCTGCCCTGAGACCCCTCAACAATGCATCAGCCTCTCCCCCTGGCCTGGGGGCCCAACCACTACCTGG TCATCTGCCCTCTCCCCACGCCATGGGACAGGGTATGGGTGGACTTCCTCCTGGCCCAGAGAAGGGCCCA ACTCTGGCTCCTTCACCCCACTCTCTGCCTCCTGCTTCCTCTTCTGCTCCAGCGCCCCCCATGAGGTTTC CTTATTCATCCTCTAGTAGTAGCTCTGCAGCAGCCTCCTCTTCCAGTTCTTCCTCCTCTTCCTCTGCCTC CCCCTTCCCAGCTTCCCAGGCATTGCCCAGCTACCCCCACTCTTTCCCTCCCCCAACAAGCCTCTCTGTC TCCAATCAGCCCCCCAAGTATACTCAGCCTTCTCTCCCATCCCAGGCTGTGTGGAGCCAGGGTCCCCCAC CACCTCCTCCCTATGGCCGCCTCTTAGCCAACAGCAATGCCCATCCAGGCCCCTTCCCTCCCTCTACTGG GGCCCAGTCCACCGCCCACCCACCAGTCTCAACACATCACCATCACCACCAGCAACAGCAACAGCAGCAG CAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCATCACGGAAACTCTGGGCCCCCTCCTCCTG GAGCATTTCCCCACCCACTGGAGGGCGGTAGCTCCCACCACGCACACCCTTACGCCATGTCTCCCTCCCT GGGGTCTCTGAGGCCCTACCCACCAGGGCCAGCACACCTGCCCCCACCTCACAGCCAGGTGTCCTACAGC CAAGCAGGCCCCAATGGCCCTCCAGTCTCTTCCTCTTCCAACTCTTCCTCTTCCACTTCTCAAGGGTCCT ACCCATGTTCACACCCCTCCCCTTCCCAGGGCCCTCAAGGGGCGCCCTACCCTTTCCCACCGGTGCCTAC GGTCACCACCTCTTCGGCTACCCTTTCCACGGTCATTGCCACCGTGGCTTCCTCGCCAGCAGGCTACAAA ACGGCCTCCCCACCTGGGCCCCCACCGTACGGAAAGAGAGCCCCGTCCCCGGGGGCCTACAAGACAGCCA CCCCACCCGGATACAAACCCGGGTCGCCTCCCTCCTTCCGAACGGGGACCCCACCGGGCTATCGAGGAAC CTCGCCACCTGCAGGCCCAGGGACCTTCAAGCCGGGCTCGCCCACCGTGGGACCTGGGCCCCTGCCACCT GCGGGGCCCTCAGGCCTGCCATCGCTGCCACCACCACCTGCGGCCCCTGCCTCAGGGCCGCCCCTGAGCG CCACGCAGATCAAACAGGAGCCGGCTGAGGAGTATGAGACCCCCGAGAGCCCGGTGCCCCCAGCCCGCAG CCCCTCGCCCCCTCCCAAGGTGGTAGATGTACCCAGCCATGCCAGTCAGTCTGCCAGGTTCAACAAACAC CTGGATCGCGGCTTCAACTCGTGCGCGCGCAGCGACCTGTACTTCGTGCCACTGGAGGGCTCCAAGCTGG CCAAGAAGCGGGCCGACCTGGTGGAGAAGGTGCGGCGCGAGGCCGAGCAGCGAGCGCGCGAAGAAAAGGA GCGCGAGCGCGAGCGGGAACGCGAGAAAGAGCGCGAGCGCGAGAAGGAGCGCGAGCTTGAACGCAGCGTG AAGTTGGCTCAGGAGGGCCGTGCTCCGGTGGAATGCCCATCTCTGGGCCCAGTGCCCCATCGCCCTCCAT TTGAACCGGGCAGTGCGGTGGCTACAGTGCCCCCCTACCTGGGTCCTGACACTCCAGCCTTGCGCACTCT CAGTGAATATGCCCGGCCTCATGTCATGTCTCCTGGCAATCGCAACCATCCATTCTACGTGCCCCTGGGG GCAGTGGACCCGGGGCTCCTGGGTTACAATGTCCCGGCCCTGTACAGCAGTGATCCAGCTGCCCGGGAGA GGGAACGGGAAGCCCGTGAACGAGACCTCCGTGACCGCCTCAAGCCTGGCTTTGAGGTGAAGCCTAGTGA GCTGGAACCCCTACATGGGGTCCCTGGGCCGGGCTTGGATCCCTTTCCCCGACATGGGGGCCTGGCTCTG CAGCCTGGCCCACCTGGCCTGCACCCTTTCCCCTTTCATCCGAGCCTGGGGCCCCTGGAGCGAGAACGTC TAGCGCTGGCAGCTGGGCCAGCCCTGCGGCCTGACATGTCCTATGCTGAGCGGCTGGCAGCTGAGAGGCA GCACGCAGAAAGGGTGGCGGCCCTGGGCAATGACCCACTGGCCCGGCTGCAGATGCTCAATGTGACTCCC CATCACCACCAGCACTCCCACATCCACTCGCACCTGCACCTGCACCAGCAAGATGCTATCCATGCAGCCT CTGCCTCGGTGCACCCTCTCATTGACCCCCTGGCCTCAGGGTCTCACCTTACCCGGATCCCCTACCCAGC TGGAACTCTCCCTAACCCCCTGCTTCCTCACCCTCTGCACGAGAACGAAGTTCTTCGTCACCAGCTCTTT GCTGCCCCTTACCGGGACCTGCCGGCCTCCCTTTCTGCCCCGATGTCAGCAGCTCATCAGCTGCAGGCCA TGCACGCACAGTCAGCTGAGCTGCAGCGCTTGGCGCTGGAACAGCAGCAGTGGCTGCATGCCCATCACCC GCTGCACAGTGTGCCGCTGCCTGCCCAGGAGGACTACTACAGTCACCTGAAGAAGGAAAGCGACAAGCCA CTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG208519 representing NM_001940
Red=Cloning site Green=Tags(s) MKTRQNKDSMSMRSGRKKEAPGPREELRSRGRASPGGVSTSSSDGKAEKSRQTAKKARVEEASTPKVNKQ GRSEEISESESEETNAPKKTKTEQELPRPQSPSDLDSLDGRSLNDDGSSDPRDIDQDNRSTSPSIYSPGS VENDSDSSSGLSQGPARPYHPPPLFPPSPQPPDSTPRQPEASFEPHPSVTPTGYHAPMEPPTSRMFQAPP GAPPPHPQLYPGGTGGVLSGPPMGPKGGGAASSVGGPNGGKQHPPPTTPISVSSSGASGAPPTKPPTTPV GGGNLPSAPPPANFPHVTPNLPPPPALRPLNNASASPPGLGAQPLPGHLPSPHAMGQGMGGLPPGPEKGP TLAPSPHSLPPASSSAPAPPMRFPYSSSSSSSAAASSSSSSSSSSASPFPASQALPSYPHSFPPPTSLSV SNQPPKYTQPSLPSQAVWSQGPPPPPPYGRLLANSNAHPGPFPPSTGAQSTAHPPVSTHHHHHQQQQQQQ QQQQQQQQQQQQQHHGNSGPPPPGAFPHPLEGGSSHHAHPYAMSPSLGSLRPYPPGPAHLPPPHSQVSYS QAGPNGPPVSSSSNSSSSTSQGSYPCSHPSPSQGPQGAPYPFPPVPTVTTSSATLSTVIATVASSPAGYK TASPPGPPPYGKRAPSPGAYKTATPPGYKPGSPPSFRTGTPPGYRGTSPPAGPGTFKPGSPTVGPGPLPP AGPSGLPSLPPPPAAPASGPPLSATQIKQEPAEEYETPESPVPPARSPSPPPKVVDVPSHASQSARFNKH LDRGFNSCARSDLYFVPLEGSKLAKKRADLVEKVRREAEQRAREEKEREREREREKEREREKERELERSV KLAQEGRAPVECPSLGPVPHRPPFEPGSAVATVPPYLGPDTPALRTLSEYARPHVMSPGNRNHPFYVPLG AVDPGLLGYNVPALYSSDPAAREREREARERDLRDRLKPGFEVKPSELEPLHGVPGPGLDPFPRHGGLAL QPGPPGLHPFPFHPSLGPLERERLALAAGPALRPDMSYAERLAAERQHAERVAALGNDPLARLQMLNVTP HHHQHSHIHSHLHLHQQDAIHAASASVHPLIDPLASGSHLTRIPYPAGTLPNPLLPHPLHENEVLRHQLF AAPYRDLPASLSAPMSAAHQLQAMHAQSAELQRLALEQQQWLHAHHPLHSVPLPAQEDYYSHLKKESDKP L TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001940 |
ORF Size | 3570 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001940.3, NP_001931.2 |
RefSeq Size | 4360 bp |
RefSeq ORF | 3573 bp |
Locus ID | 1822 |
UniProt ID | P54259 |
Protein Families | Druggable Genome |
Gene Summary | Dentatorubral pallidoluysian atrophy (DRPLA) is a rare neurodegenerative disorder characterized by cerebellar ataxia, myoclonic epilepsy, choreoathetosis, and dementia. The disorder is related to the expansion from 7-35 copies to 49-93 copies of a trinucleotide repeat (CAG/CAA) within this gene. The encoded protein includes a serine repeat and a region of alternating acidic and basic amino acids, as well as the variable glutamine repeat. Alternative splicing results in two transcripts variants that encode the same protein. [provided by RefSeq, Jul 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC208519 | ATN1 (Myc-DDK-tagged)-Human atrophin 1 (ATN1), transcript variant 2 |
CNY 8,296.00 |
|
RC208519L3 | Lenti-ORF clone of ATN1 (Myc-DDK-tagged)-Human atrophin 1 (ATN1), transcript variant 2 |
CNY 13,780.00 |
|
RC208519L4 | Lenti-ORF clone of ATN1 (mGFP-tagged)-Human atrophin 1 (ATN1), transcript variant 2 |
CNY 13,780.00 |
|
SC303096 | ATN1 (untagged)-Human atrophin 1 (ATN1), transcript variant 2 |
CNY 10,450.00 |
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