RHOJ (NM_020663) Human Tagged ORF Clone
CAT#: RG209395
- TrueORF®
RHOJ (tGFP-tagged) - Human ras homolog gene family, member J (RHOJ)
ORF Plasmid: DDK
"NM_020663" in other vectors (6)
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CNY 4,000.00
CNY 4,370.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | ARHJ; RASL7B; TC10B; TCL |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG209395 representing NM_020663
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTGCAAAGAGGGAACTGACAGCAGCTGCGGCTGCAGGGGCAACGACGAGAAGAAGATGTTGAAGT GTGTGGTGGTGGGGGACGGTGCCGTGGGGAAAACCTGCCTGCTGATGAGCTACGCCAACGACGCCTTCCC AGAGGAATACGTGCCCACTGTGTTTGACCACTATGCAGTTACTGTGACTGTGGGAGGCAAGCAACACTTG CTCGGACTGTATGACACCGCGGGACAGGAGGACTACAACCAGCTGAGGCCACTCTCCTACCCCAACACGG ATGTGTTTTTGATCTGCTTCTCTGTCGTAAACCCTGCCTCTTACCACAATGTCCAGGAGGAATGGGTCCC CGAGCTCAAGGACTGCATGCCTCACGTGCCTTATGTCCTCATAGGGACCCAGATTGATCTCCGTGATGAC CCAAAAACCTTGGCCCGTTTGCTGTATATGAAAGAGAAACCTCTCACTTACGAGCATGGTGTGAAGCTCG CAAAAGCGATCGGAGCACAGTGCTACTTGGAATGTTCAGCTCTGACTCAGAAAGGTCTCAAAGCGGTTTT TGATGAAGCAATCCTCACCATTTTCCACCCCAAGAAAAAGAAGAAACGCTGTTCTGAGGGTCACAGCTGC TGTTCAATTATC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG209395 representing NM_020663
Red=Cloning site Green=Tags(s) MNCKEGTDSSCGCRGNDEKKMLKCVVVGDGAVGKTCLLMSYANDAFPEEYVPTVFDHYAVTVTVGGKQHL LGLYDTAGQEDYNQLRPLSYPNTDVFLICFSVVNPASYHNVQEEWVPELKDCMPHVPYVLIGTQIDLRDD PKTLARLLYMKEKPLTYEHGVKLAKAIGAQCYLECSALTQKGLKAVFDEAILTIFHPKKKKKRCSEGHSC CSII TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_020663 |
ORF Size | 642 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_020663.5 |
RefSeq Size | 1907 bp |
RefSeq ORF | 645 bp |
Locus ID | 57381 |
UniProt ID | Q9H4E5 |
Domains | ras, RAS, RHO, RAB |
Protein Families | Druggable Genome |
Gene Summary | This gene encodes one of the many small GTP-binding proteins in the Rho family shown to be associated with focal adhesions in endothelial cells (PMID: 21148427, 22103495). The encoded protein is activated by vascular endothelial growth factor and may regulate angiogenesis. [provided by RefSeq, Dec 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC209395 | RHOJ (Myc-DDK-tagged)-Human ras homolog gene family, member J (RHOJ) |
CNY 2,400.00 |
|
RC209395L1 | Lenti ORF clone of Human ras homolog gene family, member J (RHOJ), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC209395L2 | Lenti ORF clone of Human ras homolog gene family, member J (RHOJ), mGFP tagged |
CNY 5,890.00 |
|
RC209395L3 | Lenti ORF clone of Human ras homolog gene family, member J (RHOJ), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC209395L4 | Lenti ORF clone of Human ras homolog gene family, member J (RHOJ), mGFP tagged |
CNY 5,890.00 |
|
SC102669 | RHOJ (untagged)-Human ras homolog gene family, member J (RHOJ) |
CNY 2,400.00 |