DBC1 (BRINP1) (NM_014618) Human Tagged ORF Clone

CAT＃: RG209538

TrueORF^®

DBC1 (tGFP-tagged) - Human deleted in bladder cancer 1 (DBC1)

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_014618" in other vectors (4)

Need custom modification / cloning service?
Get a free quote

CNY 4,370.00

货期*

3周

规格

Product images

经常一起买 (5)

PS100010

pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug

CNY 6,080.00

TA150041

Mouse monoclonal turboGFP antibody, clone OTI2H8

CNY 1,000.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA349868

Rabbit Polyclonal Anti-BRINP1 Antibody

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	TurboGFP
Synonyms	DBC1; DBCCR1; FAM5A
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RG209538 representing NM_014618 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTGGAGGTTTGTTGAGCTCCTCTACTTCCTGTTTATATGGGGCCGTATCTCAGTGCAGCCCTCCC ACCAGGAACCAGCTGGGACAGACCAACATGTCTCCAAGGAATTTGATTGGCTCATTTCAGACAGGGGGCC TTTCCACCACTCCAGGAGCTACCTATCCTTTGTGGAAAGACACCGTCAAGGATTTACAACCAGATATAAA ATATACAGGGAGTTTGCCCGTTGGAAGGTGAGGAACACAGCCATCGAGAGGAGAGATCTGGTCCGCCATC CAGTGCCCCTCATGCCGGAGTTTCAAAGGAGCATCCGCCTGCTTGGCAGGAGACCTACCACTCAGCAGTT CATCGATACCATCATCAAAAAGTACGGCACCCACCTGCTCATCTCAGCCACATTGGGAGGGGAGGAGGCT TTGACCATGTATATGGACAAAAGTCGCCTCGACAGGAAGTCAGGGAATGCCACTCAAAGTGTTGAAGCTC TGCACCAGCTCGCATCATCCTACTTTGTTGACCGTGATGGTACCATGAGGAGGCTTCATGAGATCCAGAT ATCAACTGGAGCAATCAAGGTCACAGAGACACGCACTGGGCCTCTGGGCTGTAACAGTTATGACAATCTG GACTCTGTGAGTTCCGTCCTTCTGCAAAGCACGGAGAGCAAACTGCACCTTCAAGGTCTTCAGATAATCT TTCCTCAGTATCTGCAAGAGAAGTTTGTCCAGTCGGCCTTGAGCTATATCATGTGCAATGGGGAGGGGGA GTACCTGTGCCAGAACAGCCAGTGTCGCTGCCAATGTGCCGAGGAGTTTCCGCAGTGCAACTGCCCCATC ACGGACATCCAGATCATGGAGTACACGCTGGCCAACATGGCCAAGTCTTGGGCCGAAGCTTATAAGGACC TGGAGAATTCAGATGAGTTTAAATCATTTATGAAGCGCCTCCCCAGCAACCACTTCCTGACCATCGGAAG CATCCATCAGCACTGGGGCAATGACTGGGACCTGCAGAACCGCTACAAGCTCCTGCAGAGTGCCACGGAG GCACAGAGACAAAAGATCCAACGCACTGCCCGCAAGCTTTTCGGCCTCAGTGTACGCTGTCGCCACAATC CCAACCACCAGCTGCCTAGAGAGAGGACAATTCAGCAGTGGCTTGCAAGGGTCCAGTCACTCCTCTACTG TAATGAGAATGGGTTTTGGGGAACCTTCCTGGAGAGCCAGCGGAGCTGCGTGTGCCACGGCAGCACCACG CTGTGCCAGCGCCCCATCCCCTGCGTGATAGGCGGGAACAACAGCTGCGCCATGTGCAGCCTGGCCAACA TCTCCCTCTGCGGCTCCTGCAACAAGGGCTACAAGCTGTATCGAGGCCGCTGTGAACCACAGAACGTGGA CTCGGAGCGGAGCGAGCAGTTCATCAGCTTTGAGACTGACCTGGACTTCCAGGACCTGGAGCTGAAGTAC CTGCTGCAGAAGATGGACTCACGCCTCTACGTCCACACCACCTTCATCAGCAACGAGATCCGCCTCGACA CCTTCTTTGACCCTCGGTGGCGCAAGCGCATGTCCCTCACTCTCAAGAGCAACAAGAACCGCATGGACTT CATCCACATGGTGATCGGCATGTCCATGCGCATCTGCCAGATGCGCAACAGCAGCCTGGACCCCATGTTC TTTGTCTATGTCAACCCCTTTAGCGGGAGCCATTCGGAGGGCTGGAACATGCCCTTCGGGGAATTTGGCT ACCCACGCTGGGAGAAGATCCGTCTCCAAAACAGCCAGTGCTACAACTGGACTCTTTTGCTGGGCAATCG GTGGAAAACATTTTTCGAGACGGTCCACATCTACCTACGTAGTCGGACTCGGCTACCTACCCTACTGCGA AATGAGACTGGCCAGGGCCCCGTGGACCTGTCGGATCCCTCCAAGAGGCAGTTCTACATCAAGATCTCAG ACGTGCAGGTGTTTGGGTATAGCCTGAGGTTCAACGCCGACCTCCTGCGCAGTGCAGTGCAGCAGGTCAA CCAGTCCTACACACAGGGCGGCCAGTTCTATTCCTCTTCGTCAGTGATGCTCCTCTTGTTGGATATTCGG GACCGAATTAATCGCCTGGCCCCTCCTGTGGCCCCGGGGAAACCCCAGCTGGACTTGTTCTCCTGTATGC TGAAACACCGCCTGAAACTGACCAACAGCGAGATCATCAGGGTGAACCACGCCTTGGACCTGTACAACAC GGAGATCCTCAAACAGTCGGACCAGATGACAGCCAAACTCTGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >RG209538 representing NM_014618 Red=Cloning site Green=Tags(s) MNWRFVELLYFLFIWGRISVQPSHQEPAGTDQHVSKEFDWLISDRGPFHHSRSYLSFVERHRQGFTTRYK IYREFARWKVRNTAIERRDLVRHPVPLMPEFQRSIRLLGRRPTTQQFIDTIIKKYGTHLLISATLGGEEA LTMYMDKSRLDRKSGNATQSVEALHQLASSYFVDRDGTMRRLHEIQISTGAIKVTETRTGPLGCNSYDNL DSVSSVLLQSTESKLHLQGLQIIFPQYLQEKFVQSALSYIMCNGEGEYLCQNSQCRCQCAEEFPQCNCPI TDIQIMEYTLANMAKSWAEAYKDLENSDEFKSFMKRLPSNHFLTIGSIHQHWGNDWDLQNRYKLLQSATE AQRQKIQRTARKLFGLSVRCRHNPNHQLPRERTIQQWLARVQSLLYCNENGFWGTFLESQRSCVCHGSTT LCQRPIPCVIGGNNSCAMCSLANISLCGSCNKGYKLYRGRCEPQNVDSERSEQFISFETDLDFQDLELKY LLQKMDSRLYVHTTFISNEIRLDTFFDPRWRKRMSLTLKSNKNRMDFIHMVIGMSMRICQMRNSSLDPMF FVYVNPFSGSHSEGWNMPFGEFGYPRWEKIRLQNSQCYNWTLLLGNRWKTFFETVHIYLRSRTRLPTLLR NETGQGPVDLSDPSKRQFYIKISDVQVFGYSLRFNADLLRSAVQQVNQSYTQGGQFYSSSSVMLLLLDIR DRINRLAPPVAPGKPQLDLFSCMLKHRLKLTNSEIIRVNHALDLYNTEILKQSDQMTAKLC TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_014618
ORF Size	2283 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_014618.1, NP_055433.1
RefSeq Size	3158 bp
RefSeq ORF	2286 bp
Locus ID	1620
UniProt ID	O60477
Domains	MACPF
Gene Summary	This gene is located within a chromosomal region that shows loss of heterozygosity in some bladder cancers. It contains a 5' CpG island that may be a frequent target of hypermethylation, and it may undergo hypermethylation-based silencing in some bladder cancers. [provided by RefSeq, Jul 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
RC209538	DBC1 (Myc-DDK-tagged)-Human deleted in bladder cancer 1 (DBC1)	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,576.00
RC209538L3	Lenti ORF clone of Human deleted in bladder cancer 1 (DBC1), Myc-DDK-tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
RC209538L4	Lenti ORF clone of Human deleted in bladder cancer 1 (DBC1), mGFP tagged	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,890.00
SC114955	DBC1 (untagged)-Human deleted in bladder cancer 1 (DBC1)	10 ug 产品选项验证失败所有选项都是必需的	CNY 5,584.00 CNY 7,220.00

You may also need

Empty Vector

tGFP Antibody

其它DBC1产品

Expression Plasmids

Lentiviral Particles

CRISPR

RNAi

Proteins

Antibodies