OA1 (GPR143) (NM_000273) Human Tagged ORF Clone
CAT#: RG209560
- TrueORF®
GPR143 (tGFP-tagged) - Human G protein-coupled receptor 143 (GPR143)
ORF Plasmid: DDK
"NM_000273" in other vectors (4)
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CNY 7,088.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | NYS6; OA1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG209560 representing NM_000273
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCCCCGCGCCTAGGGACCTTCTGCTGCCCCACGCGGGACGCAGCCACGCAGCTCGTGCTGAGCT TCCAGCCGCGGGCCTTCCACGCGCTCTGCCTGGGCAGCGGCGGGCTCCGCTTGGCGCTGGGCCTTCTGCA GCTGCTGCCCGGCCGCCGGCCCGCGGGCCCCGGGTCCCCCGCGACGTCCCCGCCGGCCTCGG[AT]CCGC ATCCTGCGCGCTGCCGCTGCCTGCGACCTTCTCGGCTGCCTGGGTATGGTGATCCGGTCCACCGTGTGGT TAGGATTCCCAAATTTTGTTGACAGCGTCTCGGATATGAACCACACGGAAATTTGGCCTGCTGCTTTCTG CGTGGGGAGTGCGATGTGGATCCAGCTGTTGTACAGTGCCTGCTTCTGGTGGCTGTTTTGCTATGCAGTG GATGCTTATCTGGTGATCCGGAGATCGGCAGGACTGAGCACCATCCTGCTGTATCACATCATGGCGTGGG GCCTGGCCACCCTGCTCTGTGTGGAGGGAGCCGCCATGCTCTACTACCCTTCCGTGTCCAGGTGTGAGCG GGGCCTGGACCACGCCATCCCCCACTATGTCACCATGTACCTGCCCCTGCTGCTGGTTCTCGTGGCGAAC CCCATCCTGTTCCAAAAGACAGTGACTGCAGTGGCCTCTTTACTTAAAGGAAGACAAGGCATTTACACGG AGAACGAGAGGAGGATGGGAGCCGTGATCAAGATCCGATTTTTCAAAATCATGCTGGTTTTAATTATTTG TTGGTTGTCGAATATCATCAATGAAAGCCTTTTATTCTATCTTGAGATGCAAACAGATATCAATGGAGGT TCTTTGAAACCTGTCAGAACTGCAGCCAAGACCACATGGTTTATTATGGGAATCCTGAATCCAGCCCAGG GATTTCTCTTGTCTTTGGCCTTCTACGGCTGGACAGGATGCAGCCTGGGTTTTCAGTCTCCCAGGAAGGA GATCCAGTGGGAATCACTGACCACCTCGGCTGCTGAGGGGGCTCACCCATCCCCACTGATGCCCCATGAA AACCCTGCTTCCGGGAAGGTGTCTCAAGTGGGTGGGCAGACTTCTGACGAAGCCCTGAGCATGCTGTCTG AAGGTTCTGATGCCAGCACAATTGAAATTCACACTGCAAGTGAATCCTGCAACAAAAATGAGGGTGACCC TGCTCTCCCAACCCATGGAGACCTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG209560 representing NM_000273
Red=Cloning site Green=Tags(s) MASPRLGTFCCPTRDAATQLVLSFQPRAFHALCLGSGGLRLALGLLQLLPGRRPAGPGSPATSPPASXXR ILRAAAACDLLGCLGMVIRSTVWLGFPNFVDSVSDMNHTEIWPAAFCVGSAMWIQLLYSACFWWLFCYAV DAYLVIRRSAGLSTILLYHIMAWGLATLLCVEGAAMLYYPSVSRCERGLDHAIPHYVTMYLPLLLVLVAN PILFQKTVTAVASLLKGRQGIYTENERRMGAVIKIRFFKIMLVLIICWLSNIINESLLFYLEMQTDINGG SLKPVRTAAKTTWFIMGILNPAQGFLLSLAFYGWTGCSLGFQSPRKEIQWESLTTSAAEGAHPSPLMPHE NPASGKVSQVGGQTSDEALSMLSEGSDASTIEIHTASESCNKNEGDPALPTHGDL TRTRPLE - GFP Tag - V |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_000273 |
ORF Size | 1215 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000273.2, NP_000264.2 |
RefSeq Size | 1711 bp |
RefSeq ORF | 1215 bp |
Locus ID | 4935 |
UniProt ID | P51810 |
Protein Families | Druggable Genome, Transmembrane |
Gene Summary | This gene encodes a protein that binds to heterotrimeric G proteins and is targeted to melanosomes in pigment cells. This protein is thought to be involved in intracellular signal transduction mechanisms. Mutations in this gene cause ocular albinism type 1, also referred to as Nettleship-Falls type ocular albinism, a severe visual disorder. A related pseudogene has been identified on chromosome Y. [provided by RefSeq, Dec 2009] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC209560 | GPR143 (Myc-DDK-tagged)-Human G protein-coupled receptor 143 (GPR143) |
CNY 5,488.00 |
|
RC209560L3 | Lenti-ORF clone of GPR143 (Myc-DDK-tagged)-Human G protein-coupled receptor 143 (GPR143) |
CNY 5,890.00 |
|
RC209560L4 | Lenti-ORF clone of GPR143 (mGFP-tagged)-Human G protein-coupled receptor 143 (GPR143) |
CNY 5,890.00 |
|
SC122556 | GPR143 (untagged)-Human G protein-coupled receptor 143 (GPR143) |
CNY 5,488.00 |