GPR101 (NM_054021) Human Tagged ORF Clone
CAT#: RG210183
- TrueORF®
GPR101 (tGFP-tagged) - Human G protein-coupled receptor 101 (GPR101)
ORF Plasmid: DDK
"NM_054021" in other vectors (4)
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CNY 7,272.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | GPCR6; PAGH2; PITA2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG210183 representing NM_054021
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGTCCACCTGCACCAACAGCACGCGCGAGAGTAACAGCAGCCACACGTGCATGCCCCTCTCCAAAA TGCCCATCAGCCTGGCCCACGGCATCATCCGCTCAACCGTGCTGGTTATCTTCCTCGCCGCCTCTTTCGT CGGCAACATAGTGCTGGCGCTAGTGTTGCAGCGCAAGCCGCAGCTGCTGCAGGTGACCAACCGTTTTATC TTTAACCTCCTCGTCACCGACCTGCTGCAGATTTCGCTCGTGGCCCCCTGGGTGGTGGCCACCTCTGTGC CTCTCTTCTGGCCCCTCAACAGCCACTTCTGCACGGCCCTGGTTAGCCTCACCCACCTGTTCGCCTTCGC CAGCGTCAACACCATTGTCTTGGTGTCAGTGGATCGCTACTTGTCCATCATCCACCCTCTCTCCTACCCG TCCAAGATGACCCAGCGCCGCGGTTACCTGCTCCTCTATGGCACCTGGATTGTGGCCATCCTGCAGAGCA CTCCTCCACTCTACGGCTGGGGCCAGGCTGCCTTTGATGAGCGCAATGCTCTCTGCTCCATGATCTGGGG GGCCAGCCCCAGCTACACTATTCTCAGCGTGGTGTCCTTCATCGTCATTCCACTGATTGTCATGATTGCC TGCTACTCCGTGGTGTTCTGTGCAGCCCGGAGGCAGCATGCTCTGCTGTACAATGTCAAGAGACACAGCT TGGAAGTGCGAGTCAAGGACTGTGTGGAGAATGAGGATGAAGAGGGAGCAGAGAAGAAGGAGGAGTTCCA GGATGAGAGTGAGTTTCGCCGCCAGCATGAAGGTGAGGTCAAGGCCAAGGAGGGCAGAATGGAAGCCAAG GACGGCAGCCTGAAGGCCAAGGAAGGAAGCACGGGGACCAGTGAGAGTAGTGTAGAGGCCAGGGGCAGCG AGGAGGTCAGAGAGAGCAGCACGGTGGCCAGCGACGGCAGCATGGAGGGTAAGGAAGGCAGCACCAAAGT TGAGGAGAACAGCATGAAGGCAGACAAGGGTCGCACAGAGGTCAACCAGTGCAGCATTGACTTGGGTGAA GATGACATGGAGTTTGGTGAAGACGACATCAATTTCAGTGAGGATGACGTCGAGGCAGTGAACATCCCGG AGAGCCTCCCACCCAGTCGTCGTAACAGCAACAGCAACCCTCCTCTGCCCAGGTGCTACCAGTGCAAAGC TGCTAAAGTGATCTTCATCATCATTTTCTCCTATGTGCTATCCCTGGGGCCCTACTGCTTTTTAGCAGTC CTGGCCGTGTGGGTGGATGTCGAAACCCAGGTACCCCAGTGGGTGATCACCATAATCATCTGGCTTTTCT TCCTGCAGTGCTGCATCCACCCCTATGTCTATGGCTACATGCACAAGACCATTAAGAAGGAAATCCAGGA CATGCTGAAGAAGTTCTTCTGCAAGGAAAAGCCCCCGAAAGAAGATAGCCACCCAGACCTGCCCGGAACA GAGGGTGGGACTGAAGGCAAGATTGTCCCTTCCTACGATTCTGCTACTTTTCCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG210183 representing NM_054021
Red=Cloning site Green=Tags(s) MTSTCTNSTRESNSSHTCMPLSKMPISLAHGIIRSTVLVIFLAASFVGNIVLALVLQRKPQLLQVTNRFI FNLLVTDLLQISLVAPWVVATSVPLFWPLNSHFCTALVSLTHLFAFASVNTIVLVSVDRYLSIIHPLSYP SKMTQRRGYLLLYGTWIVAILQSTPPLYGWGQAAFDERNALCSMIWGASPSYTILSVVSFIVIPLIVMIA CYSVVFCAARRQHALLYNVKRHSLEVRVKDCVENEDEEGAEKKEEFQDESEFRRQHEGEVKAKEGRMEAK DGSLKAKEGSTGTSESSVEARGSEEVRESSTVASDGSMEGKEGSTKVEENSMKADKGRTEVNQCSIDLGE DDMEFGEDDINFSEDDVEAVNIPESLPPSRRNSNSNPPLPRCYQCKAAKVIFIIIFSYVLSLGPYCFLAV LAVWVDVETQVPQWVITIIIWLFFLQCCIHPYVYGYMHKTIKKEIQDMLKKFFCKEKPPKEDSHPDLPGT EGGTEGKIVPSYDSATFP TRTRPLE - GFP Tag - V |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_054021 |
ORF Size | 1524 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_054021.1, NP_473362.1 |
RefSeq Size | 1527 bp |
RefSeq ORF | 1527 bp |
Locus ID | 83550 |
UniProt ID | Q96P66 |
Protein Families | Druggable Genome, GPCR, Transmembrane |
Gene Summary | The protein encoded by this gene is an orphan G protein-coupled receptor of unknown function. The encoded protein is a member of a family of proteins that contain seven transmembrane domains and transduce extracellular signals through heterotrimeric G proteins. [provided by RefSeq, Sep 2011] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC210183 | GPR101 (Myc-DDK-tagged)-Human G protein-coupled receptor 101 (GPR101) |
CNY 5,672.00 |
|
RC210183L3 | Lenti-ORF clone of GPR101 (Myc-DDK-tagged)-Human G protein-coupled receptor 101 (GPR101) |
CNY 6,370.00 |
|
RC210183L4 | Lenti-ORF clone of GPR101 (mGFP-tagged)-Human G protein-coupled receptor 101 (GPR101) |
CNY 6,370.00 |
|
SC120214 | GPR101 (untagged)-Human G protein-coupled receptor 101 (GPR101) |
CNY 5,688.00 |