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MAGEB3 (NM_002365) Human Tagged ORF Clone
CAT＃: RG210237
- TrueORF®
MAGEB3 (tGFP-tagged) - Human melanoma antigen family B, 3 (MAGEB3)
ORF Plasmid: DDK
"NM_002365" in other vectors (4)
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CNY 5,256.00
Product images
CNY 1,000.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CT3.5 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG210237 representing NM_002365
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTCGGGGTCAGAAGAGTACGCTCCATGCACGTGAGAAACGCCAGCAGACCCGGGGTCAGACCCAGG ATCACCAGGGTGCTCAGATCACTGCAACTAACAAGAAAAAAGTATCCTTTTCATCCCCTCTTATTTTGGG GGCTACTATCCAGAAAAAGTCTGCTGGTAGGTCACGTAGTGCTCTCAAGAAGCCTCAGAGAGCACTATCC ACCACTACATCTGTAGATGTTTCTTACAAAAAGTCATACAAGGGAGCCAACAGCAAAATTGAGAAAAAGC AAAGCTTCTCTCAGGGTCTATCCTCCACTGTGCAGTCTCGCACAGACCCTCTAATCATGAAGACAAATAT GTTGGTGCAGTTCCTGATGGAAATGTACAAGATGAAAAAGCCCATTATGAAAGCAGATATGCTAAAAATT GTCCAAAAAAGCCATAAGAATTGCTTCCCTGAGATCCTTAAAAAAGCTTCTTTCAACATGGAGGTGGTAT TTGGTGTTGATTTAAAGAAAGTTGATTCTACCAAGGACTCCTATGTCCTTGTCAGCAAAATGGATCTCCC CAACAATGGGACAGTGACTCGTGGGAGGGGATTTCCCAAGACAGGTCTCCTGCTGAATCTCCTGGGCGTG ATCTTCATGAAGGGCAACTGTGCCACTGAGGAGAAGATCTGGGAATTCCTGAATAAGATGAGAATATATG ATGGGAAGAAACACTTCATATTTGGGGAGCCCAGAAAGCTCATCACCCAAGATTTGGTGAAGCTTAAATA CCTGGAGTACCGACAAGTGCCCAACAGTAATCCTGCACGCTATGAATTCCTGTGGGGTCCAAGAGCCCAT GCTGAAACCAGCAAGATGAAGGTCCTGGAGTTTTGGGCCAAGGTCAATAAAACTGTCCCCAGTGCGTTCC AGTTCTGGTATGAAGAGGCTTTGAGAGATGAGGAAGAAAGAGTCCAAGCTGCAGCTATGCTCAATGATGG CAGTAGTGCCATGGGCAGAAAGTGTTCCAAGGCCAAGGCTAGCAGCTCTTCCCACGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG210237 representing NM_002365
Red=Cloning site Green=Tags(s) MPRGQKSTLHAREKRQQTRGQTQDHQGAQITATNKKKVSFSSPLILGATIQKKSAGRSRSALKKPQRALS TTTSVDVSYKKSYKGANSKIEKKQSFSQGLSSTVQSRTDPLIMKTNMLVQFLMEMYKMKKPIMKADMLKI VQKSHKNCFPEILKKASFNMEVVFGVDLKKVDSTKDSYVLVSKMDLPNNGTVTRGRGFPKTGLLLNLLGV IFMKGNCATEEKIWEFLNKMRIYDGKKHFIFGEPRKLITQDLVKLKYLEYRQVPNSNPARYEFLWGPRAH AETSKMKVLEFWAKVNKTVPSAFQFWYEEALRDEEERVQAAAMLNDGSSAMGRKCSKAKASSSSHA TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002365 |
| ORF Size | 1038 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002365.4 |
| RefSeq Size | 2303 bp |
| RefSeq ORF | 1041 bp |
| Locus ID | 4114 |
| UniProt ID | O15480 |
| Gene Summary | This gene is a MAGE-B subfamily member of the MAGE gene family. MAGE family member proteins direct the expression of tumor antigens recognized on a human melanoma by autologous cytolytic T lymphocytes. There are two known clusters of MAGE genes on chromosome X. The members of the MAGE-A subfamily are located in the Xq28 region, while the members of the MAGE-B subfamily are clustered in the Xp21 region. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210237 | MAGEB3 (Myc-DDK-tagged)-Human melanoma antigen family B, 3 (MAGEB3) |
CNY 3,656.00 |
|
| RC210237L3 | Lenti ORF clone of Human melanoma antigen family B, 3 (MAGEB3), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC210237L4 | Lenti ORF clone of Human melanoma antigen family B, 3 (MAGEB3), mGFP tagged |
CNY 5,890.00 |
|
| SC303187 | MAGEB3 (untagged)-Human melanoma antigen family B, 3 (MAGEB3) |
CNY 3,656.00 |
