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SF3B4 (NM_005850) Human Tagged ORF Clone
CAT＃: RG210352
- TrueORF®
SF3B4 (tGFP-tagged) - Human splicing factor 3b, subunit 4, 49kDa (SF3B4)
ORF Plasmid: DDK
"NM_005850" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AFD1; Hsh49; SAP49; SF3b49 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG210352 representing NM_005850
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCCGGGCCGATCTCCGAGCGGAATCAGGATGCCACTGTGTACGTGGGGGGCCTGGATGAGAAGG TTAGTGAACCGCTGCTGTGGGAACTGTTTCTCCAGGCTGGACCAGTAGTCAACACCCACATGCCAAAGGA TAGAGTCACTGGCCAGCACCAAGGCTATGGCTTTGTGGAATTCTTGAGTGAGGAAGATGCTGACTATGCC ATTAAGATCATGAACATGATCAAACTCTATGGGAAGCCAATACGGGTGAACAAAGCATCAGCTCACAACA AAAACCTGGATGTAGGGGCCAACATTTTCATTGGGAACCTGGACCCTGAGATTGATGAGAAGTTGCTTTA TGATACTTTCAGCGCCTTTGGGGTCATCTTACAAACCCCCAAAATTATGCGGGACCCTGACACAGGCAAC TCCAAAGGTTATGCCTTTATTAATTTTGCTTCATTTGATGCTTCGGATGCAGCAATTGAAGCCATGAATG GGCAGTACCTCTGTAACCGTCCTATCACCGTATCTTATGCCTTCAAGAAGGACTCCAAGGGTGAGCGCCA TGGCTCAGCAGCCGAACGACTTCTGGCAGCTCAGAACCCGCTCTCCCAGGCTGATCGCCCTCATCAGCTG TTTGCAGATGCACCTCCTCCACCCTCTGCTCCCAATCCTGTGGTATCATCATTGGGGTCTGGGCTTCCTC CACCAGGCATGCCTCCTCCTGGCTCCTTCCCACCCCCAGTGCCACCTCCTGGAGCCCTCCCACCTGGGAT ACCCCCAGCCATGCCCCCACCACCTATGCCTCCTGGGGCTGCAGGACATGGCCCCCCATCGGCAGGAACC CCAGGGGCAGGACATCCTGGTCATGGACACTCACATCCTCACCCATTCCCACCGGGTGGGATGCCCCATC CAGGGATGTCTCAGATGCAGCTTGCACACCATGGCCCTCATGGCTTAGGACATCCCCACGCTGGACCCCC AGGCTCTGGGGGCCAGCCACCGCCCCGACCACCACCTGGAATGCCTCATCCTGGACCTCCTCCAATGGGC ATGCCCCCCCGAGGGCCTCCATTCGGATCTCCCATGGGTCACCCAGGTCCTATGCCTCCGCATGGTATGC GTGGACCTCCTCCACTGATGCCCCCCCATGGATACACTGGCCCTCCACGACCCCCACCCTATGGCTACCA GCGGGGGCCTCTCCCTCCACCCAGACCCACTCCCCGGCCACCAGTTCCCCCTCGAGGCCCACTTCGAGGC CCTCTCCCTCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG210352 representing NM_005850
Red=Cloning site Green=Tags(s) MAAGPISERNQDATVYVGGLDEKVSEPLLWELFLQAGPVVNTHMPKDRVTGQHQGYGFVEFLSEEDADYA IKIMNMIKLYGKPIRVNKASAHNKNLDVGANIFIGNLDPEIDEKLLYDTFSAFGVILQTPKIMRDPDTGN SKGYAFINFASFDASDAAIEAMNGQYLCNRPITVSYAFKKDSKGERHGSAAERLLAAQNPLSQADRPHQL FADAPPPPSAPNPVVSSLGSGLPPPGMPPPGSFPPPVPPPGALPPGIPPAMPPPPMPPGAAGHGPPSAGT PGAGHPGHGHSHPHPFPPGGMPHPGMSQMQLAHHGPHGLGHPHAGPPGSGGQPPPRPPPGMPHPGPPPMG MPPRGPPFGSPMGHPGPMPPHGMRGPPPLMPPHGYTGPPRPPPYGYQRGPLPPPRPTPRPPVPPRGPLRG PLPQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005850 |
| ORF Size | 1272 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005850.5 |
| RefSeq Size | 2001 bp |
| RefSeq ORF | 1275 bp |
| Locus ID | 10262 |
| UniProt ID | Q15427 |
| Domains | RRM |
| Protein Pathways | Spliceosome |
| Gene Summary | This gene encodes one of four subunits of the splicing factor 3B. The protein encoded by this gene cross-links to a region in the pre-mRNA immediately upstream of the branchpoint sequence in pre-mRNA in the prespliceosomal complex A. It also may be involved in the assembly of the B, C and E spliceosomal complexes. In addition to RNA-binding activity, this protein interacts directly and highly specifically with subunit 2 of the splicing factor 3B. This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210352 | SF3B4 (Myc-DDK-tagged)-Human splicing factor 3b, subunit 4, 49kDa (SF3B4) |
CNY 3,656.00 |
|
| RC210352L3 | Lenti-ORF clone of SF3B4 (Myc-DDK-tagged)-Human splicing factor 3b, subunit 4, 49kDa (SF3B4) |
CNY 5,890.00 |
|
| RC210352L4 | Lenti-ORF clone of SF3B4 (mGFP-tagged)-Human splicing factor 3b, subunit 4, 49kDa (SF3B4) |
CNY 5,890.00 |
|
| SC116494 | SF3B4 (untagged)-Human splicing factor 3b, subunit 4, 49kDa (SF3B4) |
CNY 7,220.00 |
