alpha 1 Glycine Receptor (GLRA1) (NM_000171) Human Tagged ORF Clone
CAT#: RG210390
- TrueORF®
GLRA1 (tGFP-tagged) - Human glycine receptor, alpha 1 (GLRA1), transcript variant 2
ORF Plasmid: DDK
"NM_000171" in other vectors (6)
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CNY 5,256.00
Cited in 1 publication. |
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | HKPX1; STHE |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG210390 representing NM_000171
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACAGCTTCAATACTCTTCGACTCTACCTTTGGGAGACCATTGTATTCTTCAGCCTTGCTGCTTCTA AGGAGGCTGAAGCTGCTCGCTCCGCACCCAAGCCTATGTCACCCTCGGATTTCCTGGATAAGCTAATGGG GAGAACCTCCGGATATGATGCCAGGATCAGGCCCAATTTTAAAGGTCCCCCAGTGAACGTGAGCTGCAAC ATTTTCATCAACAGCTTTGGTTCCATTGCTGAGACAACCATGGACTATAGGGTCAACATCTTCCTGCGGC AGCAATGGAACGACCCCCGCCTGGCCTATAATGAATACCCTGACGACTCTCTGGACCTGGACCCATCCAT GCTGGACTCCATCTGGAAACCTGACCTGTTCTTTGCCAACGAGAAGGGGGCCCACTTCCATGAGATCACC ACAGACAACAAATTGCTAAGGATCTCCCGGAATGGGAATGTCCTCTACAGCATCAGAATCACCCTGACAC TGGCCTGCCCCATGGACTTGAAGAATTTCCCCATGGATGTCCAGACATGTATCATGCAACTGGAAAGCTT TGGATATACGATGAATGACCTCATCTTTGAGTGGCAGGAACAGGGAGCCGTGCAGGTAGCAGATGGACTA ACTCTGCCCCAGTTTATCTTGAAGGAAGAGAAGGACTTGAGATACTGCACCAAGCACTACAACACAGGTA AATTCACCTGCATTGAGGCCCGGTTCCACCTGGAGCGGCAGATGGGTTACTACCTGATTCAGATGTATAT TCCCAGCCTGCTCATTGTCATCCTCTCATGGATCTCCTTCTGGATCAACATGGATGCTGCACCTGCTCGT GTGGGCCTAGGCATCACCACTGTGCTCACCATGACCACCCAGAGCTCCGGCTCTCGAGCATCTCTGCCCA AGGTGTCCTATGTGAAAGCCATTGACATTTGGATGGCAGTTTGCCTGCTCTTTGTGTTCTCAGCCCTATT AGAATATGCTGCCGTTAACTTTGTGTCTCGGCAACATAAGGAGCTGCTCCGATTCAGGAGGAAGCGGAGA CATCACAAGGAGGATGAAGCTGGAGAAGGCCGCTTTAACTTCTCTGCCTATGGGATGGGCCCAGCCTGTC TACAGGCCAAGGATGGCATCTCAGTCAAGGGCGCCAACAACAGTAACACCACCAACCCCCCTCCTGCACC ATCTAAGTCCCCAGAGGAGATGCGAAAACTCTTCATCCAGAGGGCCAAGAAGATCGACAAAATATCCCGC ATTGGCTTCCCCATGGCCTTCCTCATTTTCAACATGTTCTACTGGATCATCTACAAGATTGTCCGTAGAG AGGACGTCCACAACCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG210390 representing NM_000171
Red=Cloning site Green=Tags(s) MYSFNTLRLYLWETIVFFSLAASKEAEAARSAPKPMSPSDFLDKLMGRTSGYDARIRPNFKGPPVNVSCN IFINSFGSIAETTMDYRVNIFLRQQWNDPRLAYNEYPDDSLDLDPSMLDSIWKPDLFFANEKGAHFHEIT TDNKLLRISRNGNVLYSIRITLTLACPMDLKNFPMDVQTCIMQLESFGYTMNDLIFEWQEQGAVQVADGL TLPQFILKEEKDLRYCTKHYNTGKFTCIEARFHLERQMGYYLIQMYIPSLLIVILSWISFWINMDAAPAR VGLGITTVLTMTTQSSGSRASLPKVSYVKAIDIWMAVCLLFVFSALLEYAAVNFVSRQHKELLRFRRKRR HHKEDEAGEGRFNFSAYGMGPACLQAKDGISVKGANNSNTTNPPPAPSKSPEEMRKLFIQRAKKIDKISR IGFPMAFLIFNMFYWIIYKIVRREDVHNQ TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_000171 |
ORF Size | 1347 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000171.4 |
RefSeq Size | 1715 bp |
RefSeq ORF | 1350 bp |
Locus ID | 2741 |
UniProt ID | P23415 |
Protein Families | Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane |
Protein Pathways | Neuroactive ligand-receptor interaction |
Gene Summary | The protein encoded by this gene is a subunit of a pentameric inhibitory glycine receptor, which mediates postsynaptic inhibition in the central nervous system. Defects in this gene are a cause of startle disease (STHE), also known as hereditary hyperekplexia or congenital stiff-person syndrome. Multiple transcript variants encoding different isoforms have been found. [provided by RefSeq, Dec 2015] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Adeno-Associated Virus-RNAi of GlyRa1 and Characterization of Its Synapse-specific Inhibition in OFF alpha Transient Retinal Ganglion Cells
,Zhang, C;Rompani, SB;Roska, B;McCall, MA;,
J. Neurophysiol.
,PubMed ID 25231618
[GLRA1]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC210390 | GLRA1 (Myc-DDK-tagged)-Human glycine receptor, alpha 1 (GLRA1), transcript variant 2 |
CNY 3,656.00 |
|
RC210390L1 | Lenti ORF clone of Human glycine receptor, alpha 1 (GLRA1), transcript variant 2, Myc-DDK-tagged |
CNY 6,056.00 |
|
RC210390L2 | Lenti ORF clone of Human glycine receptor, alpha 1 (GLRA1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RC210390L3 | Lenti ORF clone of Human glycine receptor, alpha 1 (GLRA1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC210390L4 | Lenti ORF clone of Human glycine receptor, alpha 1 (GLRA1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
SC310373 | GLRA1 (untagged)-Human glycine receptor, alpha 1 (GLRA1), transcript variant 2 |
CNY 3,656.00 |
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