SPDYA (NM_182756) Human Tagged ORF Clone
CAT#: RG210559
- TrueORF®
SPDYA (tGFP-tagged) - Human speedy homolog A (Xenopus laevis) (SPDYA), transcript variant 1
ORF Plasmid: DDK
"NM_182756" in other vectors (6)
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CNY 4,000.00
CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | RINGO3; RINGOA; SPDY1; SPY1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG210559 representing NM_182756
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCACAATCAGATGTGTTGTGAGACACCACCTACTGTCACTGTTTATGTAAAATCAGGGTCAAATA GATCACATCAGCCTAAAAAGCCCATTACTCTGAAGCGTCCTATTTGTAAAGATAATTGGCAAGCATTTGA AAAAAATACACATAATAACAACAAATCTAAACGCCCCAAAGGACCTTGTCTGGTTATACAGCGTCAGGAT ATGACTGCTTTCTTTAAATTATTTGATGACGATTTAATTCAAGATTTCTTGTGGATGGACTGCTGCTGTA AAATTGCAGACAAGTATCTTTTGGCTATGACCTTTGTTTATTTCAAGAGGGCTAAATTTACTATAAGTGA GCATACCAGGATAAATTTCTTTATTGCTTTGTATCTGGCTAATACAGTTGAAGAAGATGAAGAAGAAACC AAGTACGAAATTTTTCCATGGGCTTTAGGGAAAAACTGGAGAAAATTGTTCCCTAATTTCTTAAAGTTAA GGGACCAGCTCTGGGATAGAATTGACTATAGGGCTATTGTAAGCAGGCGATGTTGTGAGGAGGTTATGGC CATTGCACCAACCCATTATATCTGGCAAAGAGAACGTTCTGTTCATCACAGTGGAGCTGTCAGAAACTAC AACAGAGATGAAGTTCAGCTGCCCCGGGGACCTAGTGCCACACCAGTAGATTGTTCACTCTGTGGTAAAA AAAGAAGATATGTTAGACTGGGATTGTCTTCATCATCATCTTTATCCAGTCATACAGCAGGGGTGACAGA AAAACATTCTCAGGACTCATACAACTCACTGTCAATGGACATAATAGGTGATCCTTCTCAAGCTTATACT GGTTCTGAAGTAGTCAATGACCATCAATCAAATAAAGGAAAGAAAACTAATTTCTTGAAGAAAGACAAAT CTATGGAGTGGTTTACAGGAAGTGAAGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG210559 representing NM_182756
Red=Cloning site Green=Tags(s) MRHNQMCCETPPTVTVYVKSGSNRSHQPKKPITLKRPICKDNWQAFEKNTHNNNKSKRPKGPCLVIQRQD MTAFFKLFDDDLIQDFLWMDCCCKIADKYLLAMTFVYFKRAKFTISEHTRINFFIALYLANTVEEDEEET KYEIFPWALGKNWRKLFPNFLKLRDQLWDRIDYRAIVSRRCCEEVMAIAPTHYIWQRERSVHHSGAVRNY NRDEVQLPRGPSATPVDCSLCGKKRRYVRLGLSSSSSLSSHTAGVTEKHSQDSYNSLSMDIIGDPSQAYT GSEVVNDHQSNKGKKTNFLKKDKSMEWFTGSEE TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_182756 |
ORF Size | 939 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_182756.2, NP_877433.2 |
RefSeq Size | 1813 bp |
RefSeq ORF | 942 bp |
Locus ID | 245711 |
UniProt ID | Q5MJ70 |
Protein Pathways | Oocyte meiosis, Progesterone-mediated oocyte maturation |
Gene Summary | Regulates the G1/S phase transition of the cell cycle by binding and activating CDK1 and CDK2 (PubMed:12972555). Contributes to CDK2 activation without promoting CDK2 phosphorylation, by inducing a conformation change of the CDK2 T-loop that obstructs the substrate-binding cleft prior to kinase activation (PubMed:28666995). Mediates cell survival during the DNA damage process through activation of CDK2 (PubMed:12839962).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC210559 | SPDYA (Myc-DDK-tagged)-Human speedy homolog A (Xenopus laevis) (SPDYA), transcript variant 1 |
CNY 2,400.00 |
|
RC210559L1 | Lenti ORF clone of Human speedy homolog A (Xenopus laevis) (SPDYA), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
RC210559L2 | Lenti ORF clone of Human speedy homolog A (Xenopus laevis) (SPDYA), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC210559L3 | Lenti ORF clone of Human speedy homolog A (Xenopus laevis) (SPDYA), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC210559L4 | Lenti ORF clone of Human speedy homolog A (Xenopus laevis) (SPDYA), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
SC307504 | SPDYA (untagged)-Human speedy homolog A (Xenopus laevis) (SPDYA), transcript variant 1 |
CNY 6,270.00 |