NLRP10 (NM_176821) Human Tagged ORF Clone
CAT#: RG211215
- TrueORF®
NLRP10 (tGFP-tagged) - Human NLR family, pyrin domain containing 10 (NLRP10)
ORF Plasmid: DDK
"NM_176821" in other vectors (6)
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CNY 6,480.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | CLR11.1; NALP10; NOD8; PAN5; PYNOD |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG211215 representing NM_176821
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCATGGCCAAGGCCAGAAAGCCCCGGGAGGCATTGCTCTGGGCCTTGAGTGACCTTGAGGAGAACG ATTTCAAGAAGTTAAAGTTCTACTTACGGGATATGACCCTGTCTGAGGGCCAGCCCCCACTGGCCAGAGG GGAGTTGGAGGGCCTGATTCCGGTGGACCTGGCAGAATTACTGATTTCAAAGTATGGAGAAAAGGAGGCT GTGAAAGTTGTCCTCAAGGGCTTGAAGGTCATGAACCTGTTGGAACTTGTGGACCAGCTCAGCCATATTT GTCTGCATGATTACAGAGAAGTATACCGAGAGCATGTGCGCTGCCTAGAGGAATGGCAGGAAGCAGGAGT CAATGGCAGATACAACCAGGTGCTCCTGGTGGCCAAGCCCAGCTCAGAGAGCCCAGAATCACTTGCCTGC CCCTTCCCGGAGCAGGAGCTGGAGTCTGTCACGGTGGAGGCTCTATTTGATTCAGGGGAAAAGCCCTCAC TGGCCCCATCCTTAGTTGTGCTACAGGGGTCGGCTGGCACTGGAAAGACAACTCTCGCCAGAAAAATGGT GTTGGACTGGGCCACCGGTACTCTGTACCCAGGCCGGTTTGATTATGTCTTTTATGTAAGCTGCAAAGAA GTGGTCCTGCTGCTGGAGAGCAAACTGGAGCAGCTCCTTTTCTGGTGCTGCGGGGACAATCAAGCCCCTG TCACAGAGATTCTGAGGCAGCCAGAGCGGCTCCTGTTCATCCTGGATGGCTTTGATGAGCTGCAGAGGCC CTTTGAAGAAAAGTTGAAGAAGAGGGGTTTGAGTCCCAAGGAGAGCCTGCTGCACCTTCTAATTAGGAGA CATACACTCCCCACGTGCTCCCTTCTCATCACCACCCGGCCCCTGGCTTTGAGGAATCTGGAGCCCTTGC TGAAACAAGCACGTCATGTCCATATCCTAGGCTTCTCTGAGGAGGAGAGGGCGAGGTACTTCAGCTCCTA TTTCACGGATGAGAAGCAAGCTGACCGTGCCTTCGACATTGTACAGAAAAATGACATTCTCTACAAAGCG TGTCAGGTTCCAGGCATTTGCTGGGTGGTCTGCTCCTGGCTGCAGGGGCAGATGGAGAGAGGCAAAGTTG TCTTAGAGACACCTAGAAACAGCACTGACATCTTCATGGCTTACGTCTCCACCTTTCTGCCGCCCGATGA TGATGGGGGCTGCTCCGAGCTTTCCCGGCACAGGGTCCTGAGGAGTCTGTGCTCCCTAGCAGCTGAAGGG ATTCAGCACCAGAGGTTCCTATTTGAAGAAGCTGAGCTCAGGAAACATAATTTAGATGGCCCCAGGCTTG CCGCTTTCCTGAGTAGTAACGACTACCAATTGGGACTTGCCATCAAGAAGTTCTACAGCTTCCGCCACAT CAGCTTCCAGGACTTTTTTCATGCCATGTCTTACCTGGTGAAAGAGGACCAAAGCCGGCTGGGGAAGGAG TCCCGCAGAGAAGTGCAAAGGCTGCTGGAGGTAAAGGAGCAGGAAGGGAATGATGAGATGACCCTCACTA TGCAGTTTTTACTGGACATCTCGAAAAAAGACAGCTTCTCGAACTTGGAGCTCAAGTTCTGCTTCAGAAT TTCTCCCTGTTTAGCGCAGGATCTGAAGCATTTTAAAGAACAGATGGAATCTATGAAGCACAACAGGACC TGGGATTTGGAATTCTCCCTGTATGAAGCTAAAATAAAGAATCTGGTAAAAGGTATTCAGATGAACAATG TATCATTCAAGATAAAACATTCAAATGAAAAGAAATCACAGAGCCAGAATTTATTTTCTGTCAAAAGCAG CTTGAGTCATGGACCTAAGGAGGAGCAAAAATGTCCTTCTGTCCATGGACAGAAGGAGGGCAAAGATAAT ATAGCAGGAACACAAAAGGAAGCTTCTACTGGAAAAGGCAGAGGGACAGAGGAAACACCAAAAAATACTT ACATA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG211215 representing NM_176821
Red=Cloning site Green=Tags(s) MAMAKARKPREALLWALSDLEENDFKKLKFYLRDMTLSEGQPPLARGELEGLIPVDLAELLISKYGEKEA VKVVLKGLKVMNLLELVDQLSHICLHDYREVYREHVRCLEEWQEAGVNGRYNQVLLVAKPSSESPESLAC PFPEQELESVTVEALFDSGEKPSLAPSLVVLQGSAGTGKTTLARKMVLDWATGTLYPGRFDYVFYVSCKE VVLLLESKLEQLLFWCCGDNQAPVTEILRQPERLLFILDGFDELQRPFEEKLKKRGLSPKESLLHLLIRR HTLPTCSLLITTRPLALRNLEPLLKQARHVHILGFSEEERARYFSSYFTDEKQADRAFDIVQKNDILYKA CQVPGICWVVCSWLQGQMERGKVVLETPRNSTDIFMAYVSTFLPPDDDGGCSELSRHRVLRSLCSLAAEG IQHQRFLFEEAELRKHNLDGPRLAAFLSSNDYQLGLAIKKFYSFRHISFQDFFHAMSYLVKEDQSRLGKE SRREVQRLLEVKEQEGNDEMTLTMQFLLDISKKDSFSNLELKFCFRISPCLAQDLKHFKEQMESMKHNRT WDLEFSLYEAKIKNLVKGIQMNNVSFKIKHSNEKKSQSQNLFSVKSSLSHGPKEEQKCPSVHGQKEGKDN IAGTQKEASTGKGRGTEETPKNTYI TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_176821 |
ORF Size | 1965 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_176821.4 |
RefSeq Size | 2020 bp |
RefSeq ORF | 1968 bp |
Locus ID | 338322 |
UniProt ID | Q86W26 |
Gene Summary | Members of the NALP protein family typically contain a NACHT domain, a NACHT-associated domain (NAD), a C-terminal leucine-rich repeat (LRR) region, and an N-terminal pyrin domain (PYD). The protein encoded by this gene belongs to the NALP protein family despite lacking the LRR region. This protein likely plays a regulatory role in the innate immune system. The protein belongs to the signal-induced multiprotein complex, the inflammasome, that activates the pro-inflammatory caspases, caspase-1 and caspase-5. Other experiments indicate that this gene acts as a multifunctional negative regulator of inflammation and apoptosis. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC211215 | NLRP10 (Myc-DDK-tagged)-Human NLR family, pyrin domain containing 10 (NLRP10) |
CNY 4,880.00 |
|
RC211215L1 | Lenti ORF clone of Human NLR family, pyrin domain containing 10 (NLRP10), Myc-DDK-tagged |
CNY 7,280.00 |
|
RC211215L2 | Lenti ORF clone of Human NLR family, pyrin domain containing 10 (NLRP10), mGFP tagged |
CNY 5,890.00 |
|
RC211215L3 | Lenti ORF clone of Human NLR family, pyrin domain containing 10 (NLRP10), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC211215L4 | Lenti ORF clone of Human NLR family, pyrin domain containing 10 (NLRP10), mGFP tagged |
CNY 5,890.00 |
|
SC309242 | NLRP10 (untagged)-Human NLR family, pyrin domain containing 10 (NLRP10) |
CNY 7,220.00 |