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NEK4 (NM_003157) Human Tagged ORF Clone
CAT＃: RG211484
- TrueORF®
NEK4 (tGFP-tagged) - Human NIMA (never in mitosis gene a)-related kinase 4 (NEK4), transcript variant 1
ORF Plasmid: DDK
"NM_003157" in other vectors (7)
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CNY 9,690.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | NRK2; pp12301; STK2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG211484 representing NM_003157
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCTGGCCGCCTACTGCTACCTGCGGGTCGTGGGCAAGGGGAGCTATGGAGAGGTGACGCTTGTGA AGCACCGGCGGGACGGCAAGCAGTATGTCATCAAAAAACTGAACCTCCGAAATGCCTCTAGCCGAGAGCG GCGAGCTGCTGAACAGGAAGCCCAGCTCTTGTCTCAGTTGAAGCATCCCAACATTGTCACCTACAAGGAG TCATGGGAAGGAGGAGATGGTCTGCTCTACATTGTCATGGGCTTCTGTGAAGGAGGTGATTTGTACCGAA AGCTCAAGGAGCAGAAAGGGCAGCTTCTGCCGGAGAATCAGGTGGTAGAGTGGTTTGTACAGATCGCCAT GGCTTTGCAGTATTTACATGAAAAACACATCCTTCATCGAGATCTGAAAACTCAAAATGTCTTCCTAACA AGAACAAACATCATCAAAGTAGGGGACCTAGGAATTGCCCGAGTGTTGGAGAACCACTGTGACATGGCTA GCACCCTCATTGGCACACCCTACTACATGAGCCCTGAATTGTTCTCAAACAAACCCTACAACTATAAGTC TGATGTTTGGGCTCTAGGATGCTGTGTCTATGAAATGGCCACCTTGAAGCATGCTTTCAATGCAAAAGAT ATGAATTCTTTAGTTTATCGGATTATTGAAGGAAAGCTGCCAGCAATGCCAAGAGATTACAGCCCAGAGC TGGCAGAACTGATAAGAACAATGCTGAGCAAAAGGCCTGAAGAAAGGCCGTCTGTGAGGAGCATCCTGAG GCAGCCTTATATAAAGCGGCAAATCTCCTTCTTTTTGGAGGCCACAAAGATAAAAACCTCCAAAAATAAC ATTAAAAATGGTGACTCTCAATCCAAGCCTTTTGCTACAGTGGTTTCTGGAGAGGCAGAATCAAATCATG AAGTAATCCACCCCCAACCACTCTCTTCTGAGGGCTCCCAGACATATATAATGGGTGAAGGCAAATGTTT GTCCCAGGAGAAACCCAGGGCCTCTGGTCTCTTGAAGTCACCTGCCAGTCTGAAAGCCCATACCTGCAAA CAGGACTTGAGCAATACCACAGAACTAGCCACAATCAGTAGCGTAAATATTGACATCTTACCTGCAAAAG GGAGGGATTCAGTGAGTGATGGCTTTGTTCAGGAGAATCAGCCAAGATATTTGGATGCCTCTAATGAGTT AGGAGGTATATGCAGTATTTCTCAAGTGGAAGAGGAGATGCTGCAGGACAACACTAAATCCAGTGCCCAG CCTGAAAACCTGATTCCCATGTGGTCCTCTGACATTGTCACTGGGGAAAAGAATGAACCAGTGAAGCCTC TGCAGCCCCTAATCAAAGAACAAAAGCCAAAGGACCAGAGTCTTGCCCTGTCGCCCAAGCTGGAGTGCAG TGGCACAATCTTGGCTCACAGCAACCTCCGCCTCCTGGGTTCAAGTGATTCTCCAGCCTCAGCCTCCCGA GTAGCTGGGATTACAGGCGTGTGCCACCACGCCCAGGATCAAGTTGCTGGTGAATGTATTATAGAAAAAC AGGGCAGAATCCACCCAGATTTACAGCCACACAACTCTGGGTCTGAACCTTCCCTGTCTCGACAGCGACG GCAAAAGAGGAGAGAACAGACTGAGCACAGAGGGGAAAAGAGACAGGTCCGCAGAGATCTCTTTGCTTTC CAAGAGTCGCCTCCTCGATTTTTGCCTTCTCATCCCATTGTTGGGAAAGTGGATGTCACATCAACACAAA AAGAGGCTGAAAACCAACGTAGAGTGGTCACTGGGTCTGTGAGCAGTTCAAGGAGCAGTGAGATGTCATC ATCAAAGGATCGACCATTATCAGCCAGAGAGAGGAGGCGACTAAAGCAGTCACAGGAAGAAATGTCCTCT TCAGGCCCTTCAGTGAGGAAAGCGTCTCTGAGTGTAGCAGGGCCAGGAAAACCCCAGGAAGAAGACCAGC CCTTGCCTGCCCGACGGCTCTCCTCTGACTGCAGCGTCACTCAGGAAAGGAAACAGATTCATTGTCTGTC TGAGGATGAGTTAAGTTCTTCTACAAGTTCAACTGATAAGTCAGATGGGGATTACGGGGAAGGGAAAGGT CAGACAAATGAAATTAATGCCTTGGTACAATTGATGACTCAGACCCTGAAACTGGATTCTAAAGAGAGCT GTGAAGATGTCCCGGTAGCAAACCCAGTGTCAGAATTCAAACTTCATCGGAAATATCGGGACACACTGAT ACTTCATGGGAAGGTTGCAGAAGAGGCAGAGGAAATCCATTTTAAAGAGCTACCTTCAGCTATTATGCCA GGTTCTGAAAAGATCAGGAGACTAGTTGAAGTCTTGAGAACTGATGTAATTCGTGGCCTGGGAGTTCAGC TTTTAGAGCAGGTGTATGATCTTTTGGAGGAGGAGGATGAATTTGATAGAGAGGTACGTTTGCGGGAGCA CATGGGTGAAAAGTATACAACTTACAGTGTGAAAGCTCGCCAGTTGAAATTTTTTGAAGAAAACATGAAT TTT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG211484 representing NM_003157
Red=Cloning site Green=Tags(s) MPLAAYCYLRVVGKGSYGEVTLVKHRRDGKQYVIKKLNLRNASSRERRAAEQEAQLLSQLKHPNIVTYKE SWEGGDGLLYIVMGFCEGGDLYRKLKEQKGQLLPENQVVEWFVQIAMALQYLHEKHILHRDLKTQNVFLT RTNIIKVGDLGIARVLENHCDMASTLIGTPYYMSPELFSNKPYNYKSDVWALGCCVYEMATLKHAFNAKD MNSLVYRIIEGKLPAMPRDYSPELAELIRTMLSKRPEERPSVRSILRQPYIKRQISFFLEATKIKTSKNN IKNGDSQSKPFATVVSGEAESNHEVIHPQPLSSEGSQTYIMGEGKCLSQEKPRASGLLKSPASLKAHTCK QDLSNTTELATISSVNIDILPAKGRDSVSDGFVQENQPRYLDASNELGGICSISQVEEEMLQDNTKSSAQ PENLIPMWSSDIVTGEKNEPVKPLQPLIKEQKPKDQSLALSPKLECSGTILAHSNLRLLGSSDSPASASR VAGITGVCHHAQDQVAGECIIEKQGRIHPDLQPHNSGSEPSLSRQRRQKRREQTEHRGEKRQVRRDLFAF QESPPRFLPSHPIVGKVDVTSTQKEAENQRRVVTGSVSSSRSSEMSSSKDRPLSARERRRLKQSQEEMSS SGPSVRKASLSVAGPGKPQEEDQPLPARRLSSDCSVTQERKQIHCLSEDELSSSTSSTDKSDGDYGEGKG QTNEINALVQLMTQTLKLDSKESCEDVPVANPVSEFKLHRKYRDTLILHGKVAEEAEEIHFKELPSAIMP GSEKIRRLVEVLRTDVIRGLGVQLLEQVYDLLEEEDEFDREVRLREHMGEKYTTYSVKARQLKFFEENMN F TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003157 |
| ORF Size | 2523 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003157.2 |
| RefSeq Size | 3698 bp |
| RefSeq ORF | 2526 bp |
| Locus ID | 6787 |
| UniProt ID | A5YM70 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | The protein encoded by this gene is a serine/threonine protein kinase required for normal entry into replicative senescence. The encoded protein also is involved in cell cycle arrest in response to double-stranded DNA damage. Finally, this protein plays a role in maintaining cilium integrity, and defects in this gene have been associated with ciliopathies. [provided by RefSeq, Jan 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211484 | NEK4 (Myc-DDK-tagged)-Human NIMA (never in mitosis gene a)-related kinase 4 (NEK4), transcript variant 1 |
CNY 9,240.00 |
|
| RC211484L1 | Lenti-ORF clone of NEK4 (Myc-DDK-tagged)-Human NIMA (never in mitosis gene a)-related kinase 4 (NEK4), transcript variant 1 |
CNY 11,640.00 |
|
| RC211484L2 | Lenti-ORF clone of NEK4 (mGFP-tagged)-Human NIMA (never in mitosis gene a)-related kinase 4 (NEK4), transcript variant 1 |
CNY 10,740.00 |
|
| RC211484L3 | Lenti-ORF clone of NEK4 (Myc-DDK-tagged)-Human NIMA (never in mitosis gene a)-related kinase 4 (NEK4), transcript variant 1 |
CNY 10,740.00 |
|
| RC211484L4 | Lenti-ORF clone of NEK4 (mGFP-tagged)-Human NIMA (never in mitosis gene a)-related kinase 4 (NEK4), transcript variant 1 |
CNY 10,740.00 |
|
| SC314802 | NEK4 (untagged)-Human NIMA (never in mitosis gene a)-related kinase 4 (NEK4), transcript variant 1 |
CNY 16,340.00 |
|
| SC323353 | NEK4 (untagged)-Kinase deficient mutant (K35M) of Human NIMA (never in mitosis gene a)-related kinase 4 (NEK4) |
CNY 6,168.00 |
