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IDN3 (NIPBL) (NM_015384) Human Tagged ORF Clone
CAT＃: RG211931
- TrueORF®
NIPBL (tGFP-tagged) - Human Nipped-B homolog (Drosophila) (NIPBL), transcript variant B. Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_015384" in other vectors (2)
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CNY 20,900.00
货期*
3周
规格
| Cited in 1 publication. |
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CDLS; CDLS1; IDN3; IDN3-B; Scc2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG211931 ORF sequence, codon optimized.
Due to the complexity of NM_015384, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATGGAGATATGCCGCACGTGCCAATCACAACCCTTGCCGGGATTGCGTCACTCACGGACCTGCTTA ATCAACTCCCACTGCCCAGTCCCCTGCCAGCCACTACCACAAAGTCACTCCTCTTTAACGCTCGCATTGC CGAAGAAGTTAACTGCCTGCTCGCCTGCCGGGACGATAACTTGGTGTCACAGTTGGTACACAGCCTGAAT CAGGTGTCCACCGACCACATTGAATTGAAAGACAATCTGGGAAGCGATGACCCCGAGGGCGACATCCCCG TGCTGTTGCAGGCCGTTCTCGCCCGCTCACCTAATGTATTTAGGGAAAAGTCAATGCAGAACCGATACGT ACAGAGTGGCATGATGATGAGCCAATACAAACTCAGTCAGAATAGTATGCATTCCTCACCCGCAAGTTCA AACTACCAACAAACGACCATCTCTCATTCCCCTTCCAGTCGGTTTGTTCCACCCCAGACCTCCAGTGGGA ATCGGTTTATGCCTCAGCAGAACTCTCCGGTCCCCAGCCCCTATGCCCCTCAGAGCCCTGCTGGGTATAT GCCCTATTCACATCCGTCTAGCTATACCACCCATCCTCAGATGCAGCAGGCCAGTGTCTCATCTCCCATT GTCGCCGGAGGACTGCGCAACATCCACGATAACAAAGTCAGCGGTCCACTGTCTGGGAACAGCGCCAACC ATCACGCTGATAACCCCCGGCATGGGAGCAGCGAGGACTATCTGCATATGGTCCACCGCCTGAGCTCCGA TGATGGAGACAGTAGTACAATGAGAAACGCTGCTAGCTTTCCACTTCGGTCACCACAACCAGTGTGTTCC CCTGCGGGCTCTGAAGGGACTCCTAAAGGCAGCCGCCCTCCCCTGATCCTTCAGAGTCAATCCCTGCCAT GCAGTAGCCCCAGAGATGTGCCGCCAGATATCCTGCTGGACTCCCCCGAGCGGAAGCAGAAAAAGCAAAA AAAAATGAAGCTGGGGAAGGATGAGAAAGAACAGTCAGAGAAAGCGGCCATGTACGATATAATATCTTCT CCATCAAAGGATTCTACCAAGCTCACACTGCGACTTAGTCGAGTTCGGTCCTCCGATATGGATCAGCAGG AAGACATGATTAGCGGTGTCGAGAATTCCAACGTGAGCGAAAATGACATACCTTTTAATGTCCAGTACCC CGGCCAAACCAGTAAAACCCCCATTACACCCCAGGACATAAATCGGCCCCTGAATGCAGCTCAATGCCTT TCTCAGCAGGAACAGACGGCGTTTCTCCCCGCCAACCAGGTACCTGTTCTGCAACAGAACACTAGCGTTG CCGCAAAGCAGCCCCAGACCTCCGTGGTTCAAAATCAGCAGCAGATTTCCCAGCAAGGACCAATCTACGA TGAGGTGGAACTGGATGCATTGGCAGAGATAGAGAGAATTGAACGCGAGTCCGCCATTGAGAGGGAAAGA TTTTCCAAGGAAGTTCAGGATAAAGATAAACCACTGAAAAAGCGCAAGCAGGATAGTTACCCCCAAGAGG CGGGCGGAGCCACGGGAGGGAATAGGCCTGCCTCTCAAGAAACAGGATCTACAGGCAACGGCTCCCGCCC AGCTCTTATGGTTTCAATCGACCTGCATCAGGCGGGGCGCGTTGATAGCCAAGCGTCTATCACGCAGGAC AGCGACTCTATTAAGAAACCTGAGGAGATCAAACAGTGCAATGATGCCCCTGTCAGCGTCCTTCAGGAGG ACATAGTAGGGAGTCTCAAAAGTACCCCTGAAAACCACCCTGAGACTCCTAAGAAAAAGTCCGACCCGGA GTTGTCCAAATCAGAGATGAAACAAAGCGAAAGCCGCTTGGCAGAGAGCAAGCCTAACGAGAATCGATTG GTCGAGACAAAATCTTCAGAGAATAAGCTGGAAACCAAAGTGGAAACTCAGACTGAGGAACTCAAGCAGA ACGAGTCTCGAACTACGGAGTGTAAACAGAATGAAAGTACCATCGTGGAACCGAAACAGAATGAGAACAG ACTGAGTGACACTAAACCCAACGACAATAAGCAGAACAATGGTCGGTCTGAGACTACTAAATCCCGACCT GAGACACCAAAACAGAAAGGTGAATCCAGACCCGAGACACCCAAGCAGAAAAGCGATGGACATCCCGAAA CACCTAAACAGAAGGGAGACGGGAGGCCTGAAACTCCGAAGCAAAAGGGTGAGAGTCGGCCCGAGACGCC TAAGCAGAAGAATGAAGGCAGGCCTGAGACACCTAAGCACCGCCACGACAATCGGAGAGACAGCGGGAAG CCTTCCACGGAAAAGAAGCCAGAGGTATCTAAGCACAAGCAAGACACCAAGTCAGATAGTCCAAGACTGA AAAGTGAGCGCGCTGAGGCTCTGAAGCAGCGGCCCGACGGCCGCAGTGTGTCTGAATCATTGAGGAGAGA TCATGACAACAAGCAGAAGTCCGATGACAGAGGGGAATCTGAACGGCATCGGGGCGATCAATCCCGGGTT AGGCGGCCCGAAACTTTGCGGTCAAGTAGTAGAAATGAACACGGGATCAAAAGCGATTCTTCTAAAACCG ATAAGCTGGAGAGAAAACATAGACACGAGAGTGGGGACTCTCGGGAGAGGCCCTCCTCTGGGGAGCAGAA ATCACGCCCCGACTCACCCCGCGTGAAGCAGGGTGATAGTAACAAGTCACGGTCAGACAAACTCGGGTTC AAAAGTCCCACCTCCAAAGATGATAAAAGGACCGAAGGAAACAAAAGTAAGGTGGACACAAATAAGGCCC ATCCGGACAATAAGGCGGAATTTCCGAGCTACCTGCTGGGTGGTAGATCCGGCGCCCTGAAGAATTTCGT TATACCTAAGATTAAGAGGGATAAAGACGGAAACGTGACACAGGAGACAAAAAAAATGGAAATGAAAGGG GAGCCCAAGGACAAGGTTGAAAAAATCGGCCTTGTGGAGGACCTCAATAAAGGGGCGAAGCCTGTTGTGG TGCTTCAGAAGTTGTCCCTGGATGATGTGCAAAAACTGATAAAAGATAGAGAAGATAAGTCCCGCAGCAG CCTTAAGCCAATCAAGAACAAACCTAGCAAATCTAACAAAGGGTCTATCGACCAATCTGTGCTTAAGGAA TTGCCTCCCGAACTCCTGGCCGAAATCGAGTCTACGATGCCGCTCTGCGAAAGGGTAAAAATGAATAAGC GAAAACGGAGTACGGTGAACGAAAAACCTAAATACGCCGAAATTTCTAGCGATGAGGACAACGATTCAGA CGAGGCATTTGAGAGCAGCCGCAAGCGCCACAAGAAGGACGATGATAAAGCATGGGAGTACGAAGAGCGA GACAGGCGGAGCTCTGGGGATCACCGCAGATCAGGGCATAGTCACGAAGGCCGGCGCAGTTCCGGAGGGG GCAGATATCGGAACAGGAGTCCCTCTGATTCCGACATGGAGGATTATTCTCCCCCACCCAGCCTTAGTGA GGTTGCTCGCAAAATGAAAAAGAAGGAAAAACAAAAAAAGAGGAAGGCTTATGAGCCTAAGCTCACACCC GAAGAAATGATGGATTCTTCAACCTTCAAACGCTTTACTGCCTCCATCGAAAACATCCTCGACAACCTGG AAGACATGGACTTCACGGCCTTTGGCGACGATGACGAAATCCCCCAGGAACTGCTGCTGGGCAAGCACCA ACTGAATGAGCTTGGGAGCGAATCTGCTAAAATCAAGGCCATGGGCATAATGGACAAGCTGAGTACAGAC AAGACTGTCAAGGTCCTCAATATACTGGAAAAAAACATCCAGGACGGGTCCAAACTCTCAACGCTCCTTA ATCATAACAATGACACAGAGGAGGAGGAGCGACTGTGGAGGGACTTGATCATGGAAAGGGTTACCAAGTC TGCTGATGCCTGCCTTACCACTATCAATATAATGACCAGTCCCAATATGCCCAAAGCTGTTTACATAGAA GACGTAATTGAGCGCGTGATCCAATACACCAAGTTCCATCTGCAGAACACACTGTACCCTCAATATGACC CCGTGTACAGACTCGACCCCCATGGGGGGGGACTGCTGTCCTCTAAAGCAAAGCGCGCTAAATGTTCTAC ACATAAACAGCGCGTCATCGTGATGCTGTACAATAAGGTCTGCGATATCGTCTCTAGCCTGTCTGAGCTC CTCGAGATTCAGCTGCTGACAGACACTACGATTCTGCAGGTGTCCTCTATGGGGATTACCCCATTCTTCG TCGAGAATGTCTCAGAACTGCAACTGTGTGCAATAAAACTTGTCACAGCCGTGTTCTCCAGATACGAAAA ACACCGGCAGCTCATCCTTGAAGAAATCTTTACAAGCCTTGCCCGCCTGCCTACCTCTAAGAGAAGCCTC CGCAATTTTCGACTGAACTCAAGCGACATGGATGGAGAGCCAATGTACATCCAGATGGTGACCGCACTGG TACTTCAGCTGATCCAGTGTGTGGTCCACCTCCCAAGCAGTGAGAAGGACAGCAACGCAGAGGAAGACAG CAACAAAAAAATAGACCAGGACGTGGTGATCACCAACTCTTATGAAACAGCTATGCGCACTGCACAGAAT TTCCTTAGTATTTTCCTGAAGAAATGCGGCAGTAAGCAGGGCGAAGAGGACTATCGCCCCCTGTTTGAAA ACTTCGTGCAAGACCTTCTGAGTACCGTGAACAAGCCTGAGTGGCCCGCTGCAGAATTGCTGCTCTCCCT CCTGGGCCGCCTGCTTGTTCATCAATTTTCTAATAAATCCACCGAGATGGCCTTGCGCGTAGCCTCACTG GACTACCTTGGAACCGTAGCCGCACGGCTGCGGAAAGACGCCGTTACAAGTAAGATGGATCAGGGAAGCA TCGAACGAATTCTTAAGCAGGTTAGTGGAGGCGAGGACGAAATCCAGCAGCTCCAGAAAGCGCTGTTGGA TTATCTGGATGAGAATACTGAGACTGACCCCTCACTCGTGTTCTCCCGGAAGTTCTACATTGCCCAGTGG TTTCGCGATACCACCCTGGAGACGGAAAAGGCAATGAAGTCTCAGAAAGATGAGGAATCCAGCGAGGGCA CACATCACGCTAAAGAGATCGAAACCACAGGTCAGATCATGCATCGAGCCGAAAACCGGAAGAAGTTCTT GAGGAGTATCATTAAAACCACCCCTTCCCAGTTCTCAACACTTAAAATGAACTCCGACACCGTGGACTAC GATGATGCCTGTCTTATCGTGAGATATTTGGCCTCTATGCGGCCATTCGCACAGAGCTTTGACATCTATC TGACTCAGATCCTCAGAGTCCTCGGCGAGAATGCCATTGCCGTTAGAACCAAGGCCATGAAGTGTCTGTC CGAAGTCGTCGCTGTTGATCCCTCTATTTTGGCTCGGCTCGACATGCAAAGAGGGGTCCACGGGAGGCTG ATGGACAACAGTACATCAGTTAGGGAGGCAGCCGTGGAGTTGCTCGGGAGGTTCGTGCTCTGCCGACCTC AGCTGGCCGAACAGTATTATGATATGTTGATTGAGCGCATTCTCGATACCGGAATAAGTGTGCGAAAACG GGTCATCAAAATTTTGCGCGACATATGCATTGAGCAACCCACTTTCCCTAAGATAACAGAAATGTGTGTG AAAATGATACGCAGAGTGAATGACGAAGAGGGGATTAAGAAGCTGGTCAACGAAACGTTTCAGAAGCTGT GGTTTACCCCTACCCCACACAACGATAAGGAGGCCATGACGCGCAAAATCCTGAATATCACCGACGTAGT GGCGGCATGCAGAGATACCGGGTACGACTGGTTTGAACAGTTGCTGCAGAACCTGCTGAAGTCCGAGGAG GACTCATCTTACAAACCCGTAAAAAAGGCATGCACTCAGCTGGTGGATAACCTGGTGGAGCACATACTCA AATATGAAGAGAGCCTCGCCGACAGCGATAACAAGGGCGTAAATTCCGGTAGACTGGTCGCCTGCATCAC AACTCTTTTCTTGTTCAGCAAAATTAGACCCCAACTGATGGTCAAACACGCGATGACCATGCAGCCTTAC CTGACTACTAAGTGCTCAACGCAAAATGACTTCATGGTAATATGTAACGTGGCAAAAATTCTGGAACTGG TGGTGCCCCTGATGGAACATCCATCTGAAACTTTTCTCGCTACTATCGAGGAAGACTTGATGAAACTGAT TATAAAGTATGGGATGACTGTGGTCCAACATTGCGTCAGCTGTCTTGGAGCTGTGGTTAATAAGGTTACG CAGAACTTCAAGTTCGTTTGGGCGTGCTTCAACCGCTACTATGGGGCTATATCCAAACTGAAGAGCCAAC ATCAAGAAGACCCGAATAATACCTCTCTGCTGACTAACAAACCCGCGTTGCTGCGGAGTTTGTTCACAGT CGGAGCTTTGTGCAGACACTTCGACTTCGACCTGGAGGACTTCAAAGGGAACAGCAAAGTGAACATCAAG GATAAGGTCCTGGAACTGCTTATGTACTTCACCAAACACTCCGATGAGGAGGTGCAAACCAAGGCCATCA TCGGACTCGGCTTTGCGTTTATCCAGCATCCTTCTTTGATGTTCGAGCAGGAGGTGAAGAATCTTTACAA TAACATCCTGTCAGACAAGAACTCCAGCGTGAACCTCAAGATACAGGTGTTGAAGAACCTCCAGACTTAC CTTCAGGAGGAAGACACGAGAATGCAGCAAGCTGACAGGGATTGGAAGAAGGTGGCCAAGCAGGAGGACT TGAAAGAGATGGGCGACGTCAGCAGCGGGATGTCCAGCTCAATTATGCAGCTGTACCTGAAACAGGTCCT CGAAGCCTTTTTCCACACTCAGTCTAGCGTGCGACACTTCGCACTGAATGTAATTGCGCTGACTCTCAAC CAGGGCCTGATCCACCCCGTGCAGTGCGTGCCTTATCTCATAGCAATGGGCACAGATCCAGAACCCGCCA TGCGCAATAAGGCCGACCAGCAGCTGGTCGAAATCGATAAGAAGTATGCCGGCTTCATTCACATGAAGGC CGTTGCAGGAATGAAAATGAGCTACCAAGTACAGCAGGCCATAAACACATGCCTTAAAGATCCCGTCAGA GGCTTTCGCCAGGATGAAAGTAGCTCCGCCTTGTGCAGTCATCTGTATAGCATGATCCGCGGCAACAGGC AGCATCGGAGGGCCTTCCTTATATCCCTGTTGAACCTCTTTGATGACACTGCCAAGACAGACGTAACAAT GTTGCTTTATATAGCAGACAACCTGGCATGCTTCCCATACCAGACACAGGAGGAGCCACTCTTCATAATG CACCACATTGACATCACATTGTCAGTTAGTGGTAGTAATCTGCTGCAGAGTTTTAAGGAGTCTATGGTTA AGGACAAGAGGAAGGAGAGGAAAAGCTCCCCCAGTAAGGAGAACGAGAGCTCCGATTCCGAGGAGGAGGT TAGTCGCCCTCGGAAGTCCCGCAAGAGAGTTGATTCAGACTCCGACTCTGACAGTGAGGACGACATAAAT AGTGTAATGAAATGCCTCCCGGAAAATTCTGCACCCCTGATTGAGTTCGCCAACGTCAGTCAGGGCATTC TGCTGCTTCTGATGCTCAAGCAACACTTGAAAAACCTCTGCGGTTTCTCTGACTCTAAGATTCAGAAATA TTCACCCTCAGAAAGTGCAAAGGTCTACGATAAGGCCATAAACAGAAAGACTGGTGTGCACTTCCATCCG AAGCAGACCCTCGACTTCCTGCGGAGCGACATGGCCAACTCCAAAATAACCGAGGAGGTTAAACGATCCA TCGTGAAGCAATACCTGGACTTTAAGCTCCTTATGGAGCACCTTGATCCTGACGAAGAGGAGGAAGAGGG GGAAGTCAGCGCGAGTACGAATGCCCGCAATAAGGCAATTACTAGTCTCTTGGGAGGAGGGTCCCCGAAA AATAATACTGCCGCTGAAACAGAGGATGATGAAAGTGACGGAGAAGACAGGGGCGGAGGCACCAGCGGGG TTAGAAGGCGGCGCTCTCAGAGGATCAGCCAGCGAATCACG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG211931 representing NM_015384
Red=Cloning site Green=Tags(s) MNGDMPHVPITTLAGIASLTDLLNQLPLPSPLPATTTKSLLFNARIAEEVNCLLACRDDNLVSQLVHSLN QVSTDHIELKDNLGSDDPEGDIPVLLQAVLARSPNVFREKSMQNRYVQSGMMMSQYKLSQNSMHSSPASS NYQQTTISHSPSSRFVPPQTSSGNRFMPQQNSPVPSPYAPQSPAGYMPYSHPSSYTTHPQMQQASVSSPI VAGGLRNIHDNKVSGPLSGNSANHHADNPRHGSSEDYLHMVHRLSSDDGDSSTMRNAASFPLRSPQPVCS PAGSEGTPKGSRPPLILQSQSLPCSSPRDVPPDILLDSPERKQKKQKKMKLGKDEKEQSEKAAMYDIISS PSKDSTKLTLRLSRVRSSDMDQQEDMISGVENSNVSENDIPFNVQYPGQTSKTPITPQDINRPLNAAQCL SQQEQTAFLPANQVPVLQQNTSVAAKQPQTSVVQNQQQISQQGPIYDEVELDALAEIERIERESAIERER FSKEVQDKDKPLKKRKQDSYPQEAGGATGGNRPASQETGSTGNGSRPALMVSIDLHQAGRVDSQASITQD SDSIKKPEEIKQCNDAPVSVLQEDIVGSLKSTPENHPETPKKKSDPELSKSEMKQSESRLAESKPNENRL VETKSSENKLETKVETQTEELKQNESRTTECKQNESTIVEPKQNENRLSDTKPNDNKQNNGRSETTKSRP ETPKQKGESRPETPKQKSDGHPETPKQKGDGRPETPKQKGESRPETPKQKNEGRPETPKHRHDNRRDSGK PSTEKKPEVSKHKQDTKSDSPRLKSERAEALKQRPDGRSVSESLRRDHDNKQKSDDRGESERHRGDQSRV RRPETLRSSSRNEHGIKSDSSKTDKLERKHRHESGDSRERPSSGEQKSRPDSPRVKQGDSNKSRSDKLGF KSPTSKDDKRTEGNKSKVDTNKAHPDNKAEFPSYLLGGRSGALKNFVIPKIKRDKDGNVTQETKKMEMKG EPKDKVEKIGLVEDLNKGAKPVVVLQKLSLDDVQKLIKDREDKSRSSLKPIKNKPSKSNKGSIDQSVLKE LPPELLAEIESTMPLCERVKMNKRKRSTVNEKPKYAEISSDEDNDSDEAFESSRKRHKKDDDKAWEYEER DRRSSGDHRRSGHSHEGRRSSGGGRYRNRSPSDSDMEDYSPPPSLSEVARKMKKKEKQKKRKAYEPKLTP EEMMDSSTFKRFTASIENILDNLEDMDFTAFGDDDEIPQELLLGKHQLNELGSESAKIKAMGIMDKLSTD KTVKVLNILEKNIQDGSKLSTLLNHNNDTEEEERLWRDLIMERVTKSADACLTTINIMTSPNMPKAVYIE DVIERVIQYTKFHLQNTLYPQYDPVYRLDPHGGGLLSSKAKRAKCSTHKQRVIVMLYNKVCDIVSSLSEL LEIQLLTDTTILQVSSMGITPFFVENVSELQLCAIKLVTAVFSRYEKHRQLILEEIFTSLARLPTSKRSL RNFRLNSSDMDGEPMYIQMVTALVLQLIQCVVHLPSSEKDSNAEEDSNKKIDQDVVITNSYETAMRTAQN FLSIFLKKCGSKQGEEDYRPLFENFVQDLLSTVNKPEWPAAELLLSLLGRLLVHQFSNKSTEMALRVASL DYLGTVAARLRKDAVTSKMDQGSIERILKQVSGGEDEIQQLQKALLDYLDENTETDPSLVFSRKFYIAQW FRDTTLETEKAMKSQKDEESSEGTHHAKEIETTGQIMHRAENRKKFLRSIIKTTPSQFSTLKMNSDTVDY DDACLIVRYLASMRPFAQSFDIYLTQILRVLGENAIAVRTKAMKCLSEVVAVDPSILARLDMQRGVHGRL MDNSTSVREAAVELLGRFVLCRPQLAEQYYDMLIERILDTGISVRKRVIKILRDICIEQPTFPKITEMCV KMIRRVNDEEGIKKLVNETFQKLWFTPTPHNDKEAMTRKILNITDVVAACRDTGYDWFEQLLQNLLKSEE DSSYKPVKKACTQLVDNLVEHILKYEESLADSDNKGVNSGRLVACITTLFLFSKIRPQLMVKHAMTMQPY LTTKCSTQNDFMVICNVAKILELVVPLMEHPSETFLATIEEDLMKLIIKYGMTVVQHCVSCLGAVVNKVT QNFKFVWACFNRYYGAISKLKSQHQEDPNNTSLLTNKPALLRSLFTVGALCRHFDFDLEDFKGNSKVNIK DKVLELLMYFTKHSDEEVQTKAIIGLGFAFIQHPSLMFEQEVKNLYNNILSDKNSSVNLKIQVLKNLQTY LQEEDTRMQQADRDWKKVAKQEDLKEMGDVSSGMSSSIMQLYLKQVLEAFFHTQSSVRHFALNVIALTLN QGLIHPVQCVPYLIAMGTDPEPAMRNKADQQLVEIDKKYAGFIHMKAVAGMKMSYQVQQAINTCLKDPVR GFRQDESSSALCSHLYSMIRGNRQHRRAFLISLLNLFDDTAKTDVTMLLYIADNLACFPYQTQEEPLFIM HHIDITLSVSGSNLLQSFKESMVKDKRKERKSSPSKENESSDSEEEVSRPRKSRKRVDSDSDSDSEDDIN SVMKCLPENSAPLIEFANVSQGILLLLMLKQHLKNLCGFSDSKIQKYSPSESAKVYDKAINRKTGVHFHP KQTLDFLRSDMANSKITEEVKRSIVKQYLDFKLLMEHLDPDEEEEEGEVSASTNARNKAITSLLGGGSPK NNTAAETEDDESDGEDRGGGTSGVRRRRSQRISQRIT TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015384 |
| ORF Size | 8091 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015384.4, NP_056199.2 |
| RefSeq Size | 10389 bp |
| RefSeq ORF | 8094 bp |
| Locus ID | 25836 |
| UniProt ID | Q6KC79 |
| Gene Summary | This gene encodes the homolog of the Drosophila melanogaster Nipped-B gene product and fungal Scc2-type sister chromatid cohesion proteins. The Drosophila protein facilitates enhancer-promoter communication of remote enhancers and plays a role in developmental regulation. It is also homologous to a family of chromosomal adherins with broad roles in sister chromatid cohesion, chromosome condensation, and DNA repair. The human protein has a bipartite nuclear targeting sequence and a putative HEAT repeat. Condensins, cohesins and other complexes with chromosome-related functions also contain HEAT repeats. Mutations in this gene result in Cornelia de Lange syndrome, a disorder characterized by dysmorphic facial features, growth delay, limb reduction defects, and cognitive disability. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Disruption of NIPBL/Scc2 in Cornelia de Lange Syndrome provokes cohesin genome-wide redistribution with an impact in the transcriptome
,null,
Nature Communications
,PubMed ID 34315879
[NIPBL]
|
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