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RIOK3 (NM_003831) Human Tagged ORF Clone
CAT＃: RG212139
- TrueORF®
RIOK3 (tGFP-tagged) - Human RIO kinase 3 (yeast) (RIOK3)
ORF Plasmid: DDK
"NM_003831" in other vectors (6)
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CNY 7,400.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | SUDD |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG212139 representing NM_003831
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCTGGTAGGAGTGGCATCGCCTGAGCCCGGGACGGCAGCGGCCTGGGGACCCAGCAAGTGTCCAT GGGCTATTCCTCAAAATACAATATCTTGTTCTTTGGCTGATGTAATGAGTGAACAGCTGGCCAAAGAATT GCAGTTAGAAGAAGAAGCTGCCGTTTTTCCTGAAGTTGCTGTTGCTGAAGGACCATTTATTACTGGAGAA AACATTGATACTTCCAGTGACCTTATGCTGGCTCAGATGCTACAGATGGAATATGACAGAGAATATGATG CACAGCTTAGGCGTGAAGAAAAAAAATTCAATGGAGATAGCAAAGTTTCCATTTCCTTTGAAAATTATCG AAAAGTGCATCCTTATGAAGACAGCGATAGCTCTGAAGATGAGGTTGACTGGCAGGATACTCGTGATGAT CCCTACAGACCAGCAAAACCGGTTCCCACTCCTAAAAAGGGCTTTATTGGAAAAGGAAAAGATATCACCA CCAAACATGATGAAGTAGTATGTGGGAGAAAGAACACAGCAAGAATGGAAAATTTTGCACCTGAGTTTCA GGTAGGAGATGGAATTGGAATGGATTTAAAACTATCAAACCATGTTTTCAATGCTTTAAAACAACATGCC TACTCAGAAGAACGTCGAAGTGCCCGCCTACATGAGAAAAAGGAGCATTCTACAGCAGAAAAAGCAGTTG ATCCTAAGACACGTTTACTTATGTATAAAATGGTCAACTCTGGAATGTTGGAGACAATCACTGGCTGTAT TAGTACAGGAAAGGAGTCTGTTGTCTTTCATGCATATGGAGGGAGCATGGAGGATGAAAAGGAAGATAGT AAAGTTATACCTACAGAATGTGCCATCAAGGTATTTAAAACAACCCTTAATGAATTTAAGAATCGTGACA AATATATTAAAGATGATTTCAGGTTTAAAGATCGCTTCAGTAAACTAAATCCACGTAAGATCATCCGCAT GTGGGCAGAAAAAGAAATGCACAATCTCGCAAGAATGCAGAGAGCTGGAATTCCTTGTCCAACAGTTGTA CTACTGAAGAAACACATTTTAGTTATGTCTTTTATTGGCCATGATCAAGTTCCAGCCCCTAAATTAAAAG AAGTAAAGCTCAATAGTGAAGAAATGAAAGAAGCCTACTATCAAACTCTTCATTTGATGCGGCAGTTATA TCATGAATGTACGCTTGTCCATGCTGACCTCAGTGAGTATAACATGCTGTGGCATGCTGGAAAGGTCTGG TTGATCGATGTCAGTCAGTCAGTAGAACCTACCCACCCTCACGGCCTGGAGTTCTTGTTCCGGGACTGCA GGAATGTCTCGCAGTTTTTCCAGAAAGGAGGAGTCAAGGAAGCCCTTAGTGAACGAGAACTCTTCAATGC TGTTTCAGGCTTAAACATCACAGCAGATAATGAAGCTGATTTTTTAGCTGAGATAGAAGCTTTGGAGAAA ATGAATGAAGATCACGTTCAGAAGAATGGAAGGAAAGCTGCTTCATTTTTGAAAGATGATGGAGACCCAC CACTACTATATGATGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG212139 representing NM_003831
Red=Cloning site Green=Tags(s) MDLVGVASPEPGTAAAWGPSKCPWAIPQNTISCSLADVMSEQLAKELQLEEEAAVFPEVAVAEGPFITGE NIDTSSDLMLAQMLQMEYDREYDAQLRREEKKFNGDSKVSISFENYRKVHPYEDSDSSEDEVDWQDTRDD PYRPAKPVPTPKKGFIGKGKDITTKHDEVVCGRKNTARMENFAPEFQVGDGIGMDLKLSNHVFNALKQHA YSEERRSARLHEKKEHSTAEKAVDPKTRLLMYKMVNSGMLETITGCISTGKESVVFHAYGGSMEDEKEDS KVIPTECAIKVFKTTLNEFKNRDKYIKDDFRFKDRFSKLNPRKIIRMWAEKEMHNLARMQRAGIPCPTVV LLKKHILVMSFIGHDQVPAPKLKEVKLNSEEMKEAYYQTLHLMRQLYHECTLVHADLSEYNMLWHAGKVW LIDVSQSVEPTHPHGLEFLFRDCRNVSQFFQKGGVKEALSERELFNAVSGLNITADNEADFLAEIEALEK MNEDHVQKNGRKAASFLKDDGDPPLLYDE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003831 |
| ORF Size | 1557 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003831.5 |
| RefSeq Size | 3497 bp |
| RefSeq ORF | 1560 bp |
| Locus ID | 8780 |
| UniProt ID | O14730 |
| Domains | RIO |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene was first identified by the similarity of its product to the Aspergillus nidulans SUDD protein. This gene is now recognized as a member of the right open reading frame (RIO) kinase gene family. This gene encodes a serine/threonine kinase that localizes to the cytoplasm and plays a role in the processing of the pre-40 S ribosomal subunit. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2017] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212139 | RIOK3 (Myc-DDK-tagged)-Human RIO kinase 3 (yeast) (RIOK3) |
CNY 5,800.00 |
|
| RC212139L1 | Lenti-ORF clone of RIOK3 (Myc-DDK-tagged)-Human RIO kinase 3 (yeast) (RIOK3) |
CNY 7,030.00 |
|
| RC212139L2 | Lenti-ORF clone of RIOK3 (mGFP-tagged)-Human RIO kinase 3 (yeast) (RIOK3) |
CNY 8,200.00 |
|
| RC212139L3 | Lenti-ORF clone of RIOK3 (Myc-DDK-tagged)-Human RIO kinase 3 (yeast) (RIOK3) |
CNY 7,030.00 |
|
| RC212139L4 | Lenti-ORF clone of RIOK3 (mGFP-tagged)-Human RIO kinase 3 (yeast) (RIOK3) |
CNY 7,030.00 |
|
| SC109987 | RIOK3 (untagged)-Human RIO kinase 3 (yeast) (RIOK3) |
CNY 8,360.00 |
