GPR27 (NM_018971) Human Tagged ORF Clone
CAT#: RG213164
- TrueORF®
GPR27 (tGFP-tagged) - Human G protein-coupled receptor 27 (GPR27)
ORF Plasmid: DDK
"NM_018971" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | SREB1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG213164 representing NM_018971
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGAACGCGAGCGAGCCGGGTGGCAGCGGCGGCGGCGAGGCGGCCGCCCTGGGCCTCAAGCTGGCCA CGCTCAGCCTGCTGCTGTGCGTGAGCCTAGCGGGCAACGTGCTGTTCGCGCTGCTGATCGTGCGGGAGCG CAGCCTGCACCGCGCCCCGTACTACCTGCTGCTCGACCTGTGCCTGGCCGACGGGCTGCGCGCGCTCGCC TGCCTCCCGGCCGTCATGCTGGCGGCGCGGCGTGCGGCGGCCGCGGCGGGGGCGCCGCCGGGCGCGCTGG GCTGCAAGCTGCTCGCCTTCCTGGCCGCGCTCTTCTGCTTCCACGCCGCCTTCCTGCTGCTGGGCGTGGG CGTCACCCGCTACCTGGCCATCGCGCACCACCGCTTCTATGCAGAGCGCCTGGCCGGCTGGCCGTGCGCC GCCATGCTGGTGTGCGCCGCCTGGGCGCTGGCGCTGGCCGCGGCCTTCCCGCCAGTGCTGGACGGCGGTG GCGACGACGAGGACGCGCCGTGCGCCCTGGAGCAGCGGCCCGACGGCGCCCCCGGCGCGCTGGGCTTCCT GCTGCTGCTGGCCGTGGTGGTGGGCGCCACGCACCTCGTCTACCTCCGCCTGCTCTTCTTCATCCACGAC CGCCGCAAGATGCGGCCCGCGCGCCTGGTGCCCGCCGTCAGCCACGACTGGACCTTCCACGGCCCGGGCG CCACCGGCCAGGCGGCCGCCAACTGGACGGCGGGCTTCGGCCGCGGGCCCACGCCGCCCGCGCTTGTGGG CATCCGGCCCGCAGGGCCGGGCCGCGGCGCGCGCCGCCTCCTCGTGCTGGAAGAATTCAAGACGGAGAAG AGGCTGTGCAAGATGTTCTACGCCGTCACGCTGCTCTTCCTGCTCCTCTGGGGGCCCTACGTCGTGGCCA GCTACCTGCGGGTCCTGGTGCGGCCCGGCGCCGTCCCCCAGGCCTACCTGACGGCCTCCGTGTGGCTGAC CTTCGCGCAGGCCGGCATCAACCCCGTCGTGTGCTTCCTCTTCAACAGGGAGCTGAGGGACTGCTTCAGG GCCCAGTTCCCCTGCTGCCAGAGCCCCCGGACCACCCAGGCGACCCATCCCTGCGACCTGAAAGGCATTG GTTTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG213164 representing NM_018971
Red=Cloning site Green=Tags(s) MANASEPGGSGGGEAAALGLKLATLSLLLCVSLAGNVLFALLIVRERSLHRAPYYLLLDLCLADGLRALA CLPAVMLAARRAAAAAGAPPGALGCKLLAFLAALFCFHAAFLLLGVGVTRYLAIAHHRFYAERLAGWPCA AMLVCAAWALALAAAFPPVLDGGGDDEDAPCALEQRPDGAPGALGFLLLLAVVVGATHLVYLRLLFFIHD RRKMRPARLVPAVSHDWTFHGPGATGQAAANWTAGFGRGPTPPALVGIRPAGPGRGARRLLVLEEFKTEK RLCKMFYAVTLLFLLLWGPYVVASYLRVLVRPGAVPQAYLTASVWLTFAQAGINPVVCFLFNRELRDCFR AQFPCCQSPRTTQATHPCDLKGIGL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_018971 |
ORF Size | 1125 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_018971.3 |
RefSeq Size | 1128 bp |
RefSeq ORF | 1128 bp |
Locus ID | 2850 |
UniProt ID | Q9NS67 |
Domains | 7tm_1 |
Protein Families | Druggable Genome, GPCR, Transmembrane |
Gene Summary | GPR27 is a member of the G protein-coupled receptors (GPCRs), a large family of receptors that have a similar structure characterized by 7 transmembrane domains. Activation of GPCRs by extracellular stimuli such as neurotransmitters, hormones, or light induces an intracellular signaling cascade mediated by heterotrimeric GTP-binding proteins, or G proteins.[supplied by OMIM, May 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC213164 | GPR27 (Myc-DDK-tagged)-Human G protein-coupled receptor 27 (GPR27) |
CNY 3,656.00 |
|
RC213164L1 | Lenti ORF clone of Human G protein-coupled receptor 27 (GPR27), Myc-DDK-tagged |
CNY 6,056.00 |
|
RC213164L2 | Lenti ORF clone of Human G protein-coupled receptor 27 (GPR27), mGFP tagged |
CNY 5,890.00 |
|
RC213164L3 | Lenti ORF clone of Human G protein-coupled receptor 27 (GPR27), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC213164L4 | Lenti ORF clone of Human G protein-coupled receptor 27 (GPR27), mGFP tagged |
CNY 6,056.00 |
|
SC113278 | GPR27 (untagged)-Human G protein-coupled receptor 27 (GPR27) |
CNY 3,656.00 |