United States
Germany
Japan
United Kingdom
China
DACT2 (NM_214462) Human Tagged ORF Clone
CAT＃: RG213496
- TrueORF®
DACT2 (tGFP-tagged) - Human dapper, antagonist of beta-catenin, homolog 2 (Xenopus laevis) (DACT2)
ORF Plasmid: DDK
"NM_214462" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 7,272.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | bA503C24.7; C6orf116; DAPPER2; DPR2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG213496 representing NM_214462
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCTTGAGGGTCCCCAGCAGTCTGGCAGCCTTCCAGAGGAGGGCTCCAAGCCCTCAAACAGCTGTG TCCTCAGGGAGACCATGGTGCAGGCTTCTCCCAGCTCAAAGGCCCAGCAGACACCCTCAGCTCAGGACTA TGGACGAGGCAACATCATATCCCCATCCAGGATGCTGGACAAGAGCCCCTCACCGGCCTCTGGGCACTTT GCCCACCCATCCTTTGCTGCCAGCCTGAAAATGGGTCCCCCCAAGAGCAAGGCTGAAAAAATCAAGAGAA GTCCCATGGACAAGGTGCTGAGGTTTGCAAGGCAGCCGCTGCTTCTACTGGACAGGCCTGAGGGAGCCCA TGCAGCCCCCCAGCCATCCCTGGAGTGGGACCCTGCCCACTGGCCCACAGGGAGGGGCGGGCTCCAGCGG AGGCCAGCCCTGGCCTGGGAGGCACCCGGGCGCTCCTGTTCTGAGTCCACCCTCTACCCCATGCCTGTCC TCGTCCCCTTGGCAGTGGCCCCGCAGGAGAGCCACCGGACCTCAGCCCAAGCCCTGTTCCCCTTTGAGGC GTCACTGCTCACCTCAGTGGCCAGGAGGAAGCATCGCCGCTGGCAGTCCACCGTGGAGATCTCGGCCCGG GCCCGCCTGGCCAGCTGTCCTGAGTCTAACCTGGGGCCCCCCAGGCCCGTGGCCAGGAGAGCAGGTGGCC CACTGGCCCGGGGCCGTCCCTCACTGGTCCGCCAGGACGCCTACACCAGGAGCGACTCAGAGCCCTCCAA GCACTCGGCCGAGTGTGACCCGCGGTTCCCGTCAGTCATCCCGGAGACCAGCGAGGGAGAGTCCAGTGAC CACACCACCAACCGATTCGGAGACCGTGAGTCCAGCAGCAGCGACGAGGAGGGCGGCGCCCAGAGCAGGG ACTGTGACCTGGCACTGGGCTATGTGGCGGCCGGGCATGCGGAGCTGGCCTGGACCCAGGAGGCCCCGGT CAGCTCGGGGCCACTCCTGTCCCCCGTGCCCAAGCTGTGCCGTATTAAGGCCTCCAAGGCCCTGAAGAAG AAGATCCGCAGGTTCCAGCCGACGGCCCTGAAGGTCATGACCATGGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG213496 representing NM_214462
Red=Cloning site Green=Tags(s) MPLEGPQQSGSLPEEGSKPSNSCVLRETMVQASPSSKAQQTPSAQDYGRGNIISPSRMLDKSPSPASGHF AHPSFAASLKMGPPKSKAEKIKRSPMDKVLRFARQPLLLLDRPEGAHAAPQPSLEWDPAHWPTGRGGLQR RPALAWEAPGRSCSESTLYPMPVLVPLAVAPQESHRTSAQALFPFEASLLTSVARRKHRRWQSTVEISAR ARLASCPESNLGPPRPVARRAGGPLARGRPSLVRQDAYTRSDSEPSKHSAECDPRFPSVIPETSEGESSD HTTNRFGDRESSSSDEEGGAQSRDCDLALGYVAAGHAELAWTQEAPVSSGPLLSPVPKLCRIKASKALKK KIRRFQPTALKVMTMV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_214462 |
| ORF Size | 1098 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_214462.5 |
| RefSeq Size | 2942 bp |
| RefSeq ORF | 2325 bp |
| Locus ID | 168002 |
| UniProt ID | Q5SW24 |
| Gene Summary | Involved in regulation of intracellular signaling pathways during development. Negatively regulates the Nodal signaling pathway, possibly by promoting the lysosomal degradation of Nodal receptors, such as TGFBR1. May be involved in control of the morphogenetic behavior of kidney ureteric bud cells by keeping cells epithelial and restraining their mesenchymal character. May play an inhibitory role in the re-epithelialization of skin wounds by attenuating TGF-beta signaling (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213496 | DACT2 (Myc-DDK-tagged)-Human dapper, antagonist of beta-catenin, homolog 2 (Xenopus laevis) (DACT2) |
CNY 5,672.00 |
|
| RC213496L3 | Lenti-ORF clone of DACT2 (Myc-DDK-tagged)-Human dapper, antagonist of beta-catenin, homolog 2 (Xenopus laevis) (DACT2) |
CNY 7,700.00 |
|
| RC213496L4 | Lenti-ORF clone of DACT2 (mGFP-tagged)-Human dapper, antagonist of beta-catenin, homolog 2 (Xenopus laevis) (DACT2) |
CNY 7,700.00 |
|
| SC318256 | DACT2 (untagged)-Human dapper, antagonist of beta-catenin, homolog 2 (Xenopus laevis) (DACT2) |
CNY 15,010.00 |
