PAM (NM_138766) Human Tagged ORF Clone
CAT#: RG213661
- TrueORF®
PAM (tGFP-tagged) - Human peptidylglycine alpha-amidating monooxygenase (PAM), transcript variant 2
ORF Plasmid: DDK
"NM_138766" in other vectors (4)
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CNY 7,890.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | PAL; PHM |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG213661 representing NM_138766
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGGCCGCGTCCCTAGCCTGCTAGTTCTCCTTGTTTTTCCAAGCAGCTGTTTGGCTTTCCGAAGCC CACTTTCTGTCTTTAAGAGGTTTAAAGAAACTACCAGACCATTTTCCAATGAATGTCTTGGTACCACCAG ACCCGTAGTTCCTATTGATTCATCAGATTTTGCATTGGATATTCGCATGCCTGGGGTTACACCTAAACAG TCCGATACATACTTCTGCATGTCTATGCGAATACCAGTGGATGAGGAAGCCTTCGTGATTGACTTCAAGC CTCGAGCCAGCATGGATACTGTCCATCACATGTTACTTTTTGGATGCAATATGCCTTCATCCACTGGAAG TTACTGGTTTTGTGATGAAGGAACCTGTACAGATAAAGCCAATATTCTGTATGCCTGGGCGAGAAATGCT CCCCCTACCCGGCTCCCCAAAGGTGTTGGATTCAGAGTTGGAGGAGAGACTGGAAGTAAATACTTTGTAC TACAGGTACACTATGGGGATATTAGTGCTTTTAGAGATAATAACAAGGACTGTTCTGGTGTGTCCTTACA CCTCACACGTCTGCCACAGCCTTTAATTGCTGGCATGTACCTTATGATGTCTGTTGACACTGTTATCCCA GCAGGAGAAAAAGTGGTGAATTCTGACATTTCATGCCATTATAAAAATTATCCAATGCATGTCTTTGCCT ATAGAGTTCACACTCACCATTTAGGTAAGGTAGTAAGTGGATACAGAGTAAGAAATGGACAGTGGACACT GATTGGACGGCAGAGCCCTCAGCTGCCACAGGCTTTCTACCCTGTGGGGCATCCAGTTGATGTAAGTTTT GGTGACCTACTGGCTGCAAGATGTGTATTCACTGGTGAAGGAAGGACAGAAGCCACACACATTGGTGGCA CGTCTAGTGATGAAATGTGCAACTTATACATTATGTATTACATGGAAGCCAAGCATGCAGTTTCTTTCAT GACCTGTACCCAGAATGTAGCTCCAGATATGTTCAGAACCATACCACCAGAGGCCAACATTCCAATTCCC GTGAAGTCTGATATGGTTATGATGCATGAACATCATAAAGAAACAGAATATAAAGATAAGATTCCTTTAC TACAGCAGCCAAAACGAGAAGAAGAAGAAGTGTTAGACCAGGGTGATTTCTATTCACTACTTTCCAAGCT GCTAGGAGAAAGGGAAGATGTTGTTCATGTGCACAAATATAATCCTACAGAAAAGGCAGAATCAGAGTCA GACCTGGTAGCTGAGATTGCAAATGTAGTCCAAAAAAAGGATCTTGGTCGATCTGATGCCAGAGAGGGTG CAGAACATGAGAGGGGTAATGCTATTCTTGTCAGAGACAGAATTCACAAATTCCACAGACTAGTATCTAC CTTGAGGCCACCAGAGAGCAGAGTTTTCTCATTACAGCAGCCCCCACCTGGTGAAGGCACCTGGGAACCA GAACACACAGGAGATTTCCACATGGAAGAGGCACTGGATTGGCCTGGAGTATACTTGTTACCAGGCCAGG TTTCTGGGGTGGCTCTAGACCCTAAGAATAACCTGGTGATTTTCCACAGAGGTGACCATGTCTGGGATGG AAACTCGTTTGACAGCAAGTTTGTTTACCAGCAAATAGGACTCGGACCAATTGAAGAAGACACTATTCTT GTCATAGATCCAAATAATGCTGCAGTACTCCAGTCCAGTGGAAAAAATCTGTTTTACTTGCCACATGGCT TGAGTATAGATAAAGATGGGAATTATTGGGTCACAGACGTGGCTCTCCATCAGGTGTTCAAACTGGATCC AAACAATAAAGAAGGCCCTGTATTAATCCTGGGAAGGAGCATGCAACCAGGCAGTGACCAGAATCACTTC TGTCAACCCACTGATGTGGCTGTGGATCCAGGCACTGGAGCCATTTATGTATCAGATGGTTACTGCAACA GCAGGATTGTGCAGTTTTCACCAAGTGGAAAGTTCATCACACAGTGGGGAGAAGAGTCTTCAGGGAGCAG TCCTCTGCCAGGCCAGTTCACTGTTCCTCACAGCTTGGCTCTTGTGCCTCTTTTGGGCCAATTATGTGTG GCAGACCGGGAAAATGGTCGGATCCAGTGTTTTAAAACTGACACCAAAGAATTTGTGAGAGAGATTAAGC ATTCATCATTTGGAAGAAATGTATTTGCAATTTCATATATACCAGGCTTGCTCTTTGCAGTGAATGGGAA GCCTCATTTTGGGGACCAAGAACCTGTACAAGGATTTGTGATGAACTTTTCCAATGGGGAAATTATAGAC ATCTTCAAGCCAGTGCGCAAGCACTTTGATATGCCTCATGATATTGTTGCATCTGAAGATGGGACTGTGT ACATTGGAGATGCTCATACCAACACCGTGTGGAAGTTCACCTTGACTGAGAAATTGGAACATCGATCAGT TAAAAAGGCTGGCATTGAGGTCCAGGAAATCAAAGATTCTGAACACAAACTCGAGACGAGTTCAGGAAGA GTACTGGGAAGATTTAGAGGAAAGGGAAGTGGAGGCTTAAACCTTGGTAATTTCTTTGCAAGCCGTAAGG GCTACAGTCGAAAAGGGTTTGACCGGCTTAGCACTGAGGGCAGTGACCAAGAGAAAGAGGATGATGGAAG TGAATCAGAAGAGGAGTATTCAGCACCTCTGCCTGCGCTCGCACCTTCCTCCTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG213661 representing NM_138766
Red=Cloning site Green=Tags(s) MAGRVPSLLVLLVFPSSCLAFRSPLSVFKRFKETTRPFSNECLGTTRPVVPIDSSDFALDIRMPGVTPKQ SDTYFCMSMRIPVDEEAFVIDFKPRASMDTVHHMLLFGCNMPSSTGSYWFCDEGTCTDKANILYAWARNA PPTRLPKGVGFRVGGETGSKYFVLQVHYGDISAFRDNNKDCSGVSLHLTRLPQPLIAGMYLMMSVDTVIP AGEKVVNSDISCHYKNYPMHVFAYRVHTHHLGKVVSGYRVRNGQWTLIGRQSPQLPQAFYPVGHPVDVSF GDLLAARCVFTGEGRTEATHIGGTSSDEMCNLYIMYYMEAKHAVSFMTCTQNVAPDMFRTIPPEANIPIP VKSDMVMMHEHHKETEYKDKIPLLQQPKREEEEVLDQGDFYSLLSKLLGEREDVVHVHKYNPTEKAESES DLVAEIANVVQKKDLGRSDAREGAEHERGNAILVRDRIHKFHRLVSTLRPPESRVFSLQQPPPGEGTWEP EHTGDFHMEEALDWPGVYLLPGQVSGVALDPKNNLVIFHRGDHVWDGNSFDSKFVYQQIGLGPIEEDTIL VIDPNNAAVLQSSGKNLFYLPHGLSIDKDGNYWVTDVALHQVFKLDPNNKEGPVLILGRSMQPGSDQNHF CQPTDVAVDPGTGAIYVSDGYCNSRIVQFSPSGKFITQWGEESSGSSPLPGQFTVPHSLALVPLLGQLCV ADRENGRIQCFKTDTKEFVREIKHSSFGRNVFAISYIPGLLFAVNGKPHFGDQEPVQGFVMNFSNGEIID IFKPVRKHFDMPHDIVASEDGTVYIGDAHTNTVWKFTLTEKLEHRSVKKAGIEVQEIKDSEHKLETSSGR VLGRFRGKGSGGLNLGNFFASRKGYSRKGFDRLSTEGSDQEKEDDGSESEEEYSAPLPALAPSSS TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_138766 |
ORF Size | 2715 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_138766.2, NP_620121.1 |
RefSeq Size | 5152 bp |
RefSeq ORF | 2718 bp |
Locus ID | 5066 |
UniProt ID | P19021 |
Domains | Cu2_monoox_C, NHL |
Protein Families | Druggable Genome, Transmembrane |
Gene Summary | This gene encodes a multifunctional protein. The encoded preproprotein is proteolytically processed to generate the mature enzyme. This enzyme includes two domains with distinct catalytic activities, a peptidylglycine alpha-hydroxylating monooxygenase (PHM) domain and a peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL) domain. These catalytic domains work sequentially to catalyze the conversion of neuroendocrine peptides to active alpha-amidated products. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC213661 | PAM (Myc-DDK-tagged)-Human peptidylglycine alpha-amidating monooxygenase (PAM), transcript variant 2 |
CNY 7,130.00 |
|
RC213661L3 | Lenti-ORF clone of PAM (Myc-DDK-tagged)-Human peptidylglycine alpha-amidating monooxygenase (PAM), transcript variant 2 |
CNY 9,030.00 |
|
RC213661L4 | Lenti-ORF clone of PAM (mGFP-tagged)-Human peptidylglycine alpha-amidating monooxygenase (PAM), transcript variant 2 |
CNY 9,030.00 |
|
SC314896 | PAM (untagged)-Human peptidylglycine alpha-amidating monooxygenase (PAM), transcript variant 2 |
CNY 17,390.00 |