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NUP98 (NM_016320) Human Tagged ORF Clone
CAT＃: RG213664
- TrueORF®
NUP98 (tGFP-tagged) - Human nucleoporin 98kDa (NUP98), transcript variant 1
ORF Plasmid: DDK
"NM_016320" in other vectors (2)
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CNY 19,760.00
货期*
8周
规格
| Cited in 1 publication. |
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ADIR2; NUP96; Nup98-96; NUP196 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG213664 representing NM_016320
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTTAACAAATCATTTGGAACACCCTTTGGGGGTGGCACAGGTGGCTTTGGCACAACTTCAACATTTG GACAGAATACTGGCTTTGGCACTACTAGTGGAGGGGCATTTGGAACATCTGCATTTGGTTCTAGCAACAA TACTGGAGGCCTCTTTGGAAATTCACAGACTAAACCAGGAGGATTGTTTGGAACCAGTTCATTTAGCCAG CCAGCTACCTCCACAAGCACTGGCTTTGGGTTTGGTACGTCAACAGGAACAGCAAATACCTTGTTTGGAA CTGCAAGCACAGGGACCAGTCTCTTCTCATCCCAAAACAATGCCTTTGCACAAAATAAACCAACTGGCTT TGGCAATTTTGGAACCAGTACTAGCAGTGGAGGACTCTTTGGAACCACAAATACCACCTCTAATCCTTTT GGCAGCACATCTGGCTCCCTCTTTGGGCCAAGTAGTTTTACAGCTGCTCCTACTGGGACTACTATTAAAT TTAACCCTCCAACTGGTACAGATACTATGGTCAAAGCTGGAGTTAGCACTAACATAAGTACCAAGCACCA GTGTATTACTGCTATGAAAGAATATGAAAGCAAGTCACTAGAGGAACTTCGTTTAGAGGATTATCAGGCT AACAGGAAGGGCCCACAGAACCAGGTGGGAGCAGGTACCACAACTGGCTTGTTTGGGTCTTCTCCAGCCA CTTCCAGCGCAACAGGACTCTTCAGCTCCTCCACCACTAATTCAGGCTTTGCATATGGTCAGAACAAAAC TGCCTTTGGAACTAGTACAACTGGATTTGGAACAAATCCAGGTGGTCTCTTTGGCCAACAGAATCAGCAG ACTACCAGCCTCTTCAGCAAACCATTTGGCCAGGCTACAACCACCCAGAACACTGGCTTTTCCTTTGGTA ATACCAGCACCATAGGACAGCCAAGCACCAACACCATGGGATTATTTGGAGTAACCCAAGCCTCACAGCC TGGAGGTCTTTTTGGGACAGCTACAAACACCAGCACTGGGACAGCATTTGGAACAGGAACAGGTCTCTTT GGGCAGACCAATACTGGATTTGGTGCTGTTGGTTCGACCCTGTTTGGCAATAACAAGCTTACTACATTTG GAAGCAGCACAACCAGTGCACCTTCATTTGGTACAACCAGTGGCGGGCTCTTTGGTTTTGGCACAAATAC CAGTGGGAATAGTATTTTTGGAAGTAAACCAGCACCTGGGACTCTTGGAACTGGGCTTGGTGCAGGATTT GGAACAGCTCTTGGTGCTGGACAGGCATCTTTGTTTGGGAACAACCAACCTAAGATTGGAGGGCCTCTTG GTACAGGAGCCTTTGGGGCCCCTGGATTTAATACTACGACAGCCACTTTGGGCTTTGGAGCCCCCCAGGC CCCAGTAGCTTTGACAGATCCAAATGCTTCTGCTGCCCAGCAGGCTGTTCTCCAGCAGCACATCAATAGT CTAACATACTCACCTTTTGGAGACTCTCCTCTCTTCCGGAATCCGATGTCAGACCCTAAGAAGAAGGAAG AGAGATTGAAACCAACAAATCCAGCAGCCCAGAAGGCTCTTACTACACCTACTCATTATAAACTGACACC CCGCCCTGCCACTAGAGTCCGGCCAAAGGCTTTACAAACAACAGGCACAGCCAAGTCACATCTCTTTGAT GGGCTGGATGACGATGAACCATCCCTAGCCAATGGAGCATTCATGCCCAAGAAGAGCATTAAGAAGTTGG TTTTGAAGAACCTTAATAATAGCAATCTCTTTTCTCCTGTTAATCGTGATTCAGAAAATCTAGCTTCACC ATCTGAATATCCAGAAAATGGAGAGAGATTTAGTTTCCTAAGCAAACCTGTTGATGAGAATCACCAGCAG GATGGAGATGAAGATTCCCTTGTTTCACATTTTTATACTAACCCTATTGCCAAACCTATTCCTCAAACCC CAGAAAGTGCTGGAAATAAACACAGCAACAGCAACAGTGTGGATGATACCATTGTTGCATTAAACATGCG TGCTGCTTTGCGAAATGGGCTGGAAGGAAGCAGTGAAGAAACGTCTTTTCATGATGAGTCACTTCAGGAT GACCGAGAAGAAATAGAAAATAATTCTTACCATATGCACCCAGCAGGTATTATTCTCACTAAGGTTGGTT ACTATACTATTCCATCTATGGATGACCTTGCTAAAATTACCAATGAAAAAGGAGAGTGCATTGTCTCTGA TTTCACTATTGGTCGGAAAGGTTATGGTTCAATCTATTTTGAAGGAGATGTGAATTTGACAAATCTAAAT TTGGATGATATTGTGCATATCCGGAGGAAAGAAGTAGTTGTCTACTTAGATGATAACCAAAAACCACCTG TGGGTGAAGGGCTAAATAGGAAGGCTGAAGTTACATTGGATGGAGTTTGGCCAACAGATAAAACATCTCG TTGTTTAATAAAGAGCCCAGATCGCCTTGCTGATATCAACTATGAAGGAAGATTGGAAGCAGTTTCAAGG AAACAGGGAGCTCAATTCAAAGAATACCGGCCTGAAACTGGTTCTTGGGTGTTTAAGGTCTCCCATTTTT CTAAGTATGGCCTTCAGGATTCTGATGAAGAGGAGGAGGAGCATCCGTCTAAAACTAGTACAAAGAAGTT GAAGACTGCTCCTTTGCCTCCTGCAAGCCAGACTACGCCCTTGCAGATGGCTCTTAATGGCAAACCTGCA CCTCCACCTCAGAGCCAGAGCCCAGAGGTGGAGCAGTTAGGGAGGGTTGTGGAACTGGACAGTGACATGG TAGATATCACCCAGGAGCCAGTTTTGGATACCATGTTAGAAGAGAGCATGCCTGAGGATCAGGAACCTGT GTCTGCCTCAACACATATTGCATCTTCACTGGGAATTAATCCACATGTCTTACAGATCATGAAAGCATCA TTGCTTACTGATGAAGAAGATGTAGATATGGCACTGGATCAACGCTTCAGTCGCCTGCCTTCCAAAGCAG ATACTTCTCAAGAAATCTGTTCTCCCAGACTCCCCATTTCAGCATCCCACTCGTCGAAAACTCGTTCACT AGTTGGTGGGTTACTACAATCAAAATTTACAAGTGGAGCTTTTCTTTCACCAAGTGTCTCTGTGCAAGAA TGTCGTACTCCCAGAGCAGCATCTTTAATGAATATCCCATCCACATCCTCTTGGTCTGTCCCTCCACCCC TGACTTCTGTGTTCACAATGCCCAGCCCAGCCCCTGAGGTTCCGTTGAAAACAGTGGGTACACGTAGGCA ACTAGGCCTAGTCCCTCGTGAAAAGTCTGTCACCTATGGCAAGGGAAAACTCTTGATGGACATGGCCCTA TTCATGGGACGCTCATTTCGTGTTGGTTGGGGCCCCAACTGGACTCTTGCTAATAGTGGAGAACAGCTGA ATGGCTCTCATGAACTAGAAAATCATCAGATTGCCGATTCCATGGAGTTTGGATTCCTGCCCAATCCAGT AGCTGTTAAACCCCTAACTGAGTCCCCATTCAAAGTTCACTTAGAAAAACTCAGTTTGAGACAAAGAAAG CCAGATGAAGACATGAAATTATATCAGACACCTCTGGAGCTCAAATTAAAACACAGCACTGTCCATGTGG ATGAACTGTGTCCTCTCATTGTCCCCAATCTGGGAGTTGCTGTCATTCATGACTATGCAGATTGGGTTAA AGAAGCATCAGGAGACTTACCAGAAGCACAAATTGTGAAGCACTGGAGCCTGACATGGACACTATGTGAA GCCCTATGGGGCCACCTGAAGGAGCTTGACAGCCAGCTAAATGAACCCCGTGAATACATTCAAATTCTGG AGCGAAGAAGAGCTTTCTCCCGCTGGCTATCCTGTACTGCCACACCTCAGATTGAAGAGGAAGTCTCCTT AACCCAAAAAAACAGCCCTGTGGAGGCTGTATTCAGCTACCTCACAGGCAAAAGGATCAGTGAGGCCTGC TCTCTGGCCCAGCAGTCAGGGGATCATCGTCTTGCTCTTCTTTTATCTCAGTTTGTGGGTAGCCAGTCAG TCCGGGAGCTGCTCACCATGCAGTTGGTGGACTGGCATCAGCTCCAAGCAGACTCCTTCATCCAGGATGA GAGACTGCGCATCTTTGCTCTGTTGGCTGGAAAACCGGTGTGGCAGCTCTCAGAAAAGAAGCAAATAAAC GTGTGCTCCCAGTTGGATTGGAAACGCTCCCTGGCTATCCATCTTTGGTATTTGCTTCCACCAACAGCCT CCATTTCTAGGGCGCTCAGCATGTATGAAGAAGCATTTCAGAATACCTCTGACAGTGACAGATATGCCTG CTCCCCACTTCCTTCGTATCTGGAGGGTTCTGGCTGTGTGATAGCGGAGGAGCAAAACTCACAGACACCA CTTCGAGATGTCTGCTTTCACCTTCTAAAACTCTACAGTGACAGACATTATGATCTCAACCAGCTGCTGG AGCCTCGAAGCATAACAGCAGATCCTTTGGACTACCGCCTAAGCTGGCACTTGTGGGAAGTGCTGAGGGC TCTTAACTACACCCATCTCTCAGCGCAGTGTGAAGGTGTGCTACAGGCCAGTTACGCTGGCCAGCTTGAA AGTGAGGGGCTCTGGGAGTGGGCCATCTTTGTCCTCCTGCACATTGACAACTCAGGCATACGTGAGAAGG CTGTTCGAGAGCTGCTTACCCGGCACTGCCAGCTGTTGGAGACCCCTGAATCTTGGGCTAAAGAGACTTT CCTTACCCAGAAGCTCCGTGTACCTGCCAAATGGATCCACGAGGCCAAAGCTGTGCGAGCACACATGGAA TCTGACAAGCACTTAGAGGCCCTTTGCTTATTTAAGGCTGAGCACTGGAACCGCTGCCACAAGCTCATCA TCCGACACTTAGCTTCTGATGCCATCATTAATGAGAACTATGACTACCTGAAGGGGTTCTTGGAAGACCT GGCACCTCCAGAGCGCAGCAGCCTAATTCAGGATTGGGAAACATCTGGGCTTGTTTACCTGGACTATATT AGAGTCATTGAAATGCTCCGCCATATACAGCAGGTGGATTGCTCAGGTAATGACCTGGAGCAGTTACACA TCAAAGTGACTTCACTGTGCAGTCGGATAGAGCAGATTCAGTGTTACAGTGCTAAAGATCGCCTGGCTCA GTCAGACATGGCCAAACGTGTAGCCAACCTGCTGCGCGTGGTGCTGAGTCTGCATCATCCTCCTGATAGA ACCTCCGACTCAACACCAGACCCTCAGCGAGTCCCTTTGCGCCTCTTGGCTCCCCACATTGGCCGGCTTC CCATGCCTGAGGACTATGCCATGGACGAACTGCGCAGCCTTACCCAGTCCTATCTGCGAGAACTGGCTGT TGGGAGCCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG213664 representing NM_016320
Red=Cloning site Green=Tags(s) MFNKSFGTPFGGGTGGFGTTSTFGQNTGFGTTSGGAFGTSAFGSSNNTGGLFGNSQTKPGGLFGTSSFSQ PATSTSTGFGFGTSTGTANTLFGTASTGTSLFSSQNNAFAQNKPTGFGNFGTSTSSGGLFGTTNTTSNPF GSTSGSLFGPSSFTAAPTGTTIKFNPPTGTDTMVKAGVSTNISTKHQCITAMKEYESKSLEELRLEDYQA NRKGPQNQVGAGTTTGLFGSSPATSSATGLFSSSTTNSGFAYGQNKTAFGTSTTGFGTNPGGLFGQQNQQ TTSLFSKPFGQATTTQNTGFSFGNTSTIGQPSTNTMGLFGVTQASQPGGLFGTATNTSTGTAFGTGTGLF GQTNTGFGAVGSTLFGNNKLTTFGSSTTSAPSFGTTSGGLFGFGTNTSGNSIFGSKPAPGTLGTGLGAGF GTALGAGQASLFGNNQPKIGGPLGTGAFGAPGFNTTTATLGFGAPQAPVALTDPNASAAQQAVLQQHINS LTYSPFGDSPLFRNPMSDPKKKEERLKPTNPAAQKALTTPTHYKLTPRPATRVRPKALQTTGTAKSHLFD GLDDDEPSLANGAFMPKKSIKKLVLKNLNNSNLFSPVNRDSENLASPSEYPENGERFSFLSKPVDENHQQ DGDEDSLVSHFYTNPIAKPIPQTPESAGNKHSNSNSVDDTIVALNMRAALRNGLEGSSEETSFHDESLQD DREEIENNSYHMHPAGIILTKVGYYTIPSMDDLAKITNEKGECIVSDFTIGRKGYGSIYFEGDVNLTNLN LDDIVHIRRKEVVVYLDDNQKPPVGEGLNRKAEVTLDGVWPTDKTSRCLIKSPDRLADINYEGRLEAVSR KQGAQFKEYRPETGSWVFKVSHFSKYGLQDSDEEEEEHPSKTSTKKLKTAPLPPASQTTPLQMALNGKPA PPPQSQSPEVEQLGRVVELDSDMVDITQEPVLDTMLEESMPEDQEPVSASTHIASSLGINPHVLQIMKAS LLTDEEDVDMALDQRFSRLPSKADTSQEICSPRLPISASHSSKTRSLVGGLLQSKFTSGAFLSPSVSVQE CRTPRAASLMNIPSTSSWSVPPPLTSVFTMPSPAPEVPLKTVGTRRQLGLVPREKSVTYGKGKLLMDMAL FMGRSFRVGWGPNWTLANSGEQLNGSHELENHQIADSMEFGFLPNPVAVKPLTESPFKVHLEKLSLRQRK PDEDMKLYQTPLELKLKHSTVHVDELCPLIVPNLGVAVIHDYADWVKEASGDLPEAQIVKHWSLTWTLCE ALWGHLKELDSQLNEPREYIQILERRRAFSRWLSCTATPQIEEEVSLTQKNSPVEAVFSYLTGKRISEAC SLAQQSGDHRLALLLSQFVGSQSVRELLTMQLVDWHQLQADSFIQDERLRIFALLAGKPVWQLSEKKQIN VCSQLDWKRSLAIHLWYLLPPTASISRALSMYEEAFQNTSDSDRYACSPLPSYLEGSGCVIAEEQNSQTP LRDVCFHLLKLYSDRHYDLNQLLEPRSITADPLDYRLSWHLWEVLRALNYTHLSAQCEGVLQASYAGQLE SEGLWEWAIFVLLHIDNSGIREKAVRELLTRHCQLLETPESWAKETFLTQKLRVPAKWIHEAKAVRAHME SDKHLEALCLFKAEHWNRCHKLIIRHLASDAIINENYDYLKGFLEDLAPPERSSLIQDWETSGLVYLDYI RVIEMLRHIQQVDCSGNDLEQLHIKVTSLCSRIEQIQCYSAKDRLAQSDMAKRVANLLRVVLSLHHPPDR TSDSTPDPQRVPLRLLAPHIGRLPMPEDYAMDELRSLTQSYLRELAVGSL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016320 |
| ORF Size | 5400 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016320.5 |
| RefSeq Size | 6850 bp |
| RefSeq ORF | 5403 bp |
| Locus ID | 4928 |
| UniProt ID | P52948 |
| Domains | Nucleoporin_FG, Nucleoporin2 |
| Protein Families | Druggable Genome |
| Gene Summary | Nuclear pore complexes (NPCs) regulate the transport of macromolecules between the nucleus and cytoplasm, and are composed of many polypeptide subunits, many of which belong to the nucleoporin family. This gene belongs to the nucleoporin gene family and encodes a 186 kDa precursor protein that undergoes autoproteolytic cleavage to generate a 98 kDa nucleoporin and 96 kDa nucleoporin. The 98 kDa nucleoporin contains a Gly-Leu-Phe-Gly (GLGF) repeat domain and participates in many cellular processes, including nuclear import, nuclear export, mitotic progression, and regulation of gene expression. The 96 kDa nucleoporin is a scaffold component of the NPC. Proteolytic cleavage is important for targeting of the proteins to the NPC. Translocations between this gene and many other partner genes have been observed in different leukemias. Rearrangements typically result in chimeras with the N-terminal GLGF domain of this gene to the C-terminus of the partner gene. Alternative splicing results in multiple transcript variants encoding different isoforms, at least two of which are proteolytically processed. Some variants lack the region that encodes the 96 kDa nucleoporin. [provided by RefSeq, Feb 2016] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
G4C2 Repeat RNA Initiates a POM121-Mediated Reduction in Specific Nucleoporins in C9orf72 ALS/FTD
,Coyne, AN;Zaepfel, BL;Hayes, L;Fitchman, B;Salzberg, Y;Luo, EC;Bowen, K;Trost, H;Aigner, S;Rigo, F;Yeo, GW;Harel, A;Svendsen, CN;Sareen, D;Rothstein, JD;,
Neuron
,PubMed ID 32673563
[NUP98]
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