DOCK9 (NM_015296) Human Tagged ORF Clone
CAT#: RG214044
- TrueORF®
DOCK9 (tGFP-tagged) - Human dedicator of cytokinesis 9 (DOCK9), transcript variant 1
ORF Plasmid: DDK
"NM_015296" in other vectors (2)
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CNY 15,256.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | ZIZ1; ZIZIMIN1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG214044 representing NM_015296
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGCAGCCGCCGCTGCTCCCCGCCTCGGCGGAGACTCGGAAGTTCACCCGGGCGCTGAGTAAGCCGG GCACGGCGGCCGAGCTGCGGCAGAGCGTGTCTGAGGTGGTGCGCGGCTCCGTGCTCCTGGCAAAGCCAAA GCTAATTGAGCCACTCGACTATGAAAATGTCATCGTCCAGAAGAAGACTCAGATCCTGAACGACTGTTTA CGGGAGATGCTGCTCTTCCCTTACGATGACTTTCAGACGGCCATCCTGAGACGACAGGGTCGATACATAT GCTCAACAGTGCCTGCGAAGGCGGAAGAGGAAGCACAGAGCTTGTTTGTTACAGAGTGCATCAAAACCTA TAACTCTGACTGGCATCTTGTGAACTATAAATATGAAGATTACTCAGGAGAGTTTCGACAGCTTCCGAAC AAAGTGGTCAAGTTGGATAAACTTCCAGTTCATGTCTATGAAGTTGACGAGGAGGTCGACAAAGATGAGG ATGCTGCCTCCCTTGGTTCCCAGAAGGGTGGGATCACCAAGCATGGCTGGCTGTACAAAGGCAACATGAA CAGTGCCATCAGCGTGACCATGAGGTCATTTAAGAGACGATTTTTCCACCTGATTCAACTTGGCGATGGA TCCTATAATTTGAATTTTTATAAAGATGAAAAGATCTCCAAAGAACCAAAAGGATCAATATTTCTGGATT CCTGTATGGGTGTCGTTCAGAACAACAAAGTCAGGCGTTTTGCTTTTGAGCTCAAGATGCAGGACAAAAG TAGTTATCTCTTGGCAGCAGACAGTGAAGTGGAAATGGAAGAATGGATCACAATTCTAAATAAGATCCTC CAGCTCAACTTTGAAGCTGCAATGCAAGAAAAGCGAAATGGCGACTCTCACGAAGATGATGAACAAAGCA AATTGGAAGGTTCTGGTTCCGGTTTAGATAGCTACCTGCCGGAACTTGCCAAGAGTGCAAGAGAAGCAGA AATCAAACTGAAAAGTGAAAGCAGAGTCAAACTTTTTTATTTGGACCCAGATGCCCAGAAGCTTGACTTC TCATCAGCTGAGCCAGAAGTGAAGTCATTTGAAGAGAAGTTTGGAAAAAGGATCCTTGTCAAGTGCAATG ATTTATCTTTCAATTTGCAATGCTGTGTTGCCGAAAATGAAGAAGGACCCACTACAAATGTTGAACCTTT CTTTGTTACTCTATCCCTGTTTGACATAAAATACAACCGGAAGATTTCTGCCGATTTCCACGTAGACCTG AACCATTTCTCAGTGAGGCAAATGCTCGCCACCACGTCCCCGGCGCTGATGAATGGCAGTGGGCAGAGCC CATCTGTCCTCAAGGGCATCCTTCATGAAGCCGCCATGCAGTATCCGAAGCAGGGAATATTTTCAGTCAC TTGTCCTCATCCAGATATATTTCTTGTGGCCAGAATTGAAAAAGTCCTTCAGGGGAGCATCACACATTGC GCTGAGCCATATATGAAAAGTTCAGACTCTTCTAAGGTGGCCCAGAAGGTGCTGAAGAATGCCAAGCAGG CATGCCAAAGACTAGGACAGTATAGAATGCCATTTGCTTGGGCAGCAAGGACATTGTTTAAGGATGCATC TGGAAATCTTGACAAAAATGCCAGATTTTCTGCCATCTACAGGCAAGACAGCAATAAGCTATCCAATGAT GACATGCTCAAGTTACTTGCAGACTTTCGGAAACCTGAGAAGATGGCTAAGCTCCCAGTGATTTTAGGCA ATCTAGACATTACAATTGATAATGTTTCCTCAGACTTCCCTAATTATGTTAATTCATCATACATTCCCAC AAAACAATTTGAAACCTGCAGTAAAACTCCCATCACGTTTGAAGTGGAGGAATTTGTGCCCTGCATACCA AAACACACTCAGCCTTACACCATCTACACCAATCACCTTTACGTTTATCCTAAGTACTTGAAATACGACA GTCAGAAGTCTTTTGCCAAGGCTAGAAATATTGCGATTTGCATTGAATTCAAAGATTCAGATGAGGAAGA CTCTCAGCCCCTTAAGTGCATTTATGGCAGACCTGGTGGGCCAGTTTTCACAAGAAGCGCCTTTGCTGCA GTTTTACACCATCACCAAAACCCAGAATTTTATGATGAGATTAAAATAGAGTTGCCCACTCAGCTGCATG AAAAGCACCACCTGTTGCTCACATTCTTCCATGTCAGCTGTGACAACTCAAGTAAAGGAAGCACGAAGAA GAGGGATGTCGTTGAAACCCAAGTTGGCTACTCCTGGCTTCCCCTCCTGAAAGACGGAAGGGTGGTGACA AGCGAGCAGCACATCCCGGTCTCGGCGAACCTTCCTTCGGGCTATCTTGGCTACCAGGAGCTTGGGATGG GCAGGCATTATGGTCCGGAAATTAAATGGGTAGATGGAGGCAAGCCACTGCTGAAAATTTCCACTCATCT GGTTTCTACAGTGTATACTCAGGATCAGCATTTACATAATTTTTTCCAGTACTGTCAGAAAACCGAATCT GGAGCCCAAGCCTTAGGAAACGAACTTGTAAAGTACCTTAAGAGTCTGCATGCGATGGAAGGCCACGTGA TGATCGCCTTCTTGCCCACTATCCTAAACCAGCTGTTCCGAGTCCTCACCAGAGCCACACAGGAAGAAGT CGCGGTTAACGTGACTCGGGTCATTATTCATGTGGTTGCCCAGTGCCATGAGGAAGGATTGGAGAGCCAC TTGAGGTCATATGTTAAGTACGCGTATAAGGCTGAGCCATATGTTGCCTCTGAATACAAGACAGTGCATG AAGAACTGACCAAATCCATGACCACGATTCTCAAGCCTTCTGCCGATTTCCTCACCAGCAACAAACTACT GAAGTACTCATGGTTTTTCTTTGATGTACTGATCAAATCTATGGCTCAGCATTTGATAGAGAACTCCAAA GTTAAGTTGCTGCGAAACCAGAGATTTCCTGCATCCTATCATCATGCAGTGGAAACCGTTGTAAATATGC TGATGCCACACATCACTCAGAAGTTTCGAGATAATCCAGAGGCATCTAAGAACGCGAATCATAGCCTTGC TGTCTTCATCAAGAGATGTTTCACCTTCATGGACAGGGGCTTTGTCTTCAAGCAGATCAACAACTACATT AGCTGTTTTGCTCCTGGAGACCCAAAGACCCTCTTTGAATACAAGTTTGAATTTCTCCGTGTAGTGTGCA ACCATGAACATTATATTCCGTTGAACTTACCAATGCCATTTGGAAAAGGCAGGATTCAAAGATACCAAGA CCTCCAGCTTGACTACTCATTAACAGATGAGTTCTGCAGAAACCACTTCTTGGTGGGACTGTTACTGAGG GAGGTGGGGACAGCCCTCCAGGAGTTCCGGGAGGTCCGTCTGATCGCCATCAGTGTGCTCAAGAACCTGC TGATAAAGCATTCTTTTGATGACAGATATGCTTCAAGGAGCCATCAGGCAAGGATAGCCACCCTCTACCT GCCTCTGTTTGGTCTGCTGATTGAAAACGTCCAGCGGATCAATGTGAGGGATGTGTCACCCTTCCCTGTG AACGCGGGCATGACTGTGAAGGATGAATCCCTGGCTCTACCAGCTGTGAATCCGCTGGTGACGCCGCAGA AGGGAAGCACCCTGGACAACAGCCTGCACAAGGACCTGCTGGGCGCCATCTCCGGCATTGCTTCTCCATA TACAACCTCAACTCCAAACATCAACAGTGTGAGAAATGCTGATTCGAGAGGATCTCTCATAAGCACAGAT TCGGGTAACAGCCTTCCAGAAAGGAATAGTGAGAAGAGCAATTCCCTGGATAAGCACCAACAAAGTAGCA CATTGGGAAATTCCGTGGTTCGCTGTGATAAACTTGACCAGTCTGAGATTAAGAGCCTACTGATGTGTTT CCTCTACATCTTAAAGAGCATGTCTGATGATGCTTTGTTTACATATTGGAACAAGGCTTCAACATCTGAA CTTATGGATTTTTTTACAATATCTGAAGTCTGCCTGCACCAGTTCCAGTACATGGGGAAGCGATACATAG CCAGAACAGGAATGATGCATGCCAGATTGCAGCAGCTGGGCAGCCTGGATAACTCTCTCACTTTTAACCA CAGCTATGGCCACTCGGACGCAGATGTTCTGCACCAGTCATTACTTGAAGCCAACATTGCTACTGAGGTT TGCCTGACAGCTCTGGACACGCTTTCTCTATTTACATTGGCGTTTAAGAACCAGCTCCTGGCCGACCATG GACATAATCCTCTCATGAAAAAAGTTTTTGATGTCTACCTGTGTTTTCTTCAAAAACATCAGTCTGAAAC GGCTTTAAAAAATGTCTTCACTGCCTTAAGGTCCTTAATTTATAAGTTTCCCTCAACATTCTATGAAGGG AGAGCGGACATGTGTGCGGCTCTGTGTTACGAGATTCTCAAGTGCTGTAACTCCAAGCTGAGCTCCATCA GGACGGAGGCCTCCCAGCTGCTCTACTTCCTGATGAGGAACAACTTTGATTACACTGGAAAGAAGTCCTT TGTCCGGACACATTTGCAAGTCATCATATCTGTCAGCCAGCTGATAGCAGACGTTGTTGGCATTGGGGGA ACCAGATTCCAGCAGTCCCTGTCCATCATCAACAACTGTGCCAACAGTGACCGGCTTATTAAGCACACCA GCTTCTCCTCTGATGTGAAGGACTTAACCAAAAGGATACGCACGGTGCTAATGGCCACCGCCCAGATGAA GGAGCATGAGAACGACCCAGAGATGCTGGTGGACCTCCAGTACAGCCTGGCCAAATCCTATGCCAGCACG CCCGAGCTCAGGAAGACGTGGCTCGACAGCATGGCCAGGATCCATGTCAAAAATGGCGATCTCTCAGAGG CAGCAATGTGCTATGTCCACGTAACAGCCCTAGTGGCAGAATATCTCACACGGAAAGAAGCAGTCCAGTG GGAGCCGCCCCTTCTCCCCCACAGCCATAGCGCCTGCCTGAGGAGGAGCCGGGGAGGCGTGTTTAGACAA GGATGCACCGCCTTCAGGGTCATTACCCCAAACATCGACGAGGAGGCCTCCATGATGGAAGACGTGGGGA TGCAGGATGTCCATTTCAACGAGGATGTGCTGATGGAGCTCCTTGAGCAGTGCGCAGATGGACTCTGGAA AGCCGAGCGCTACGAGCTCATTGCCGACATCTACAAACTTATCATCCCCATTTATGAGAAGCGGAGGGAT TTTGAGAGGCTGGCCCATCTGTATGACACGCTGCACCGGGCCTACAGCAAAGTGACCGAGGTCATGCACT CGGGCCGCAGGCTTCTGGGGACCTACTTCCGGGTAGCCTTCTTCGGGCAGGCAGCGCAATACCAGTTTAC AGACAGTGAAACAGATGTGGAGGGATTCTTTGAAGATGAAGATGGAAAGGAGTATATTTACAAGGAACCC AAACTCACACCGCTGTCGGAAATTTCTCAGAGACTCCTTAAACTGTACTCGGATAAATTTGGTTCTGAAA ATGTCAAAATGATACAGGATTCTGGCAAGGTCAACCCTAAGGATCTGGATTCTAAGTATGCCTACATCCA GGTGACTCACGTCATCCCCTTCTTTGACGAAAAAGAGTTGCAAGAAAGGAAAACAGAGTTTGAGAGATCC CACAACATCCGCCGCTTCATGTTTGAGATGCCATTTACGCAGACCGGGAAGAGGCAGGGCGGGGTGGAAG AGCAGTGCAAACGGCGCACCATCCTGACAGCCATACACTGCTTCCCTTATGTGAAGAAGCGCATCCCTGT CATGTACCAGCACCACACTGACCTGAACCCCATCGAGGTGGCCATTGACGAGATGAGTAAGAAGGTGGCG GAGCTCCGGCAGCTGTGCTCCTCGGCCGAGGTGGACATGATCAAACTGCAGCTCAAACTCCAGGGCAGCG TGAGTGTTCAGGTCAATGCTGGCCCACTAGCATATGCGCGAGCTTTCTTAGATGATACAAACACAAAGCG ATATCCTGACAATAAAGTGAAGCTGCTTAAGGAAGTTTTCAGGCAATTTGTGGAAGCTTGCGGTCAAGCC TTAGCGGTAAACGAACGTCTGATTAAAGAAGACCAGCTCGAGTATCAGGAAGAAATGAAAGCCAACTACA GGGAAATGGCGAAGGAGCTTTCTGAAATCATGCATGAGCAGCTGGGA AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG214044 representing NM_015296
Red=Cloning site Green=Tags(s) MSQPPLLPASAETRKFTRALSKPGTAAELRQSVSEVVRGSVLLAKPKLIEPLDYENVIVQKKTQILNDCL REMLLFPYDDFQTAILRRQGRYICSTVPAKAEEEAQSLFVTECIKTYNSDWHLVNYKYEDYSGEFRQLPN KVVKLDKLPVHVYEVDEEVDKDEDAASLGSQKGGITKHGWLYKGNMNSAISVTMRSFKRRFFHLIQLGDG SYNLNFYKDEKISKEPKGSIFLDSCMGVVQNNKVRRFAFELKMQDKSSYLLAADSEVEMEEWITILNKIL QLNFEAAMQEKRNGDSHEDDEQSKLEGSGSGLDSYLPELAKSAREAEIKLKSESRVKLFYLDPDAQKLDF SSAEPEVKSFEEKFGKRILVKCNDLSFNLQCCVAENEEGPTTNVEPFFVTLSLFDIKYNRKISADFHVDL NHFSVRQMLATTSPALMNGSGQSPSVLKGILHEAAMQYPKQGIFSVTCPHPDIFLVARIEKVLQGSITHC AEPYMKSSDSSKVAQKVLKNAKQACQRLGQYRMPFAWAARTLFKDASGNLDKNARFSAIYRQDSNKLSND DMLKLLADFRKPEKMAKLPVILGNLDITIDNVSSDFPNYVNSSYIPTKQFETCSKTPITFEVEEFVPCIP KHTQPYTIYTNHLYVYPKYLKYDSQKSFAKARNIAICIEFKDSDEEDSQPLKCIYGRPGGPVFTRSAFAA VLHHHQNPEFYDEIKIELPTQLHEKHHLLLTFFHVSCDNSSKGSTKKRDVVETQVGYSWLPLLKDGRVVT SEQHIPVSANLPSGYLGYQELGMGRHYGPEIKWVDGGKPLLKISTHLVSTVYTQDQHLHNFFQYCQKTES GAQALGNELVKYLKSLHAMEGHVMIAFLPTILNQLFRVLTRATQEEVAVNVTRVIIHVVAQCHEEGLESH LRSYVKYAYKAEPYVASEYKTVHEELTKSMTTILKPSADFLTSNKLLKYSWFFFDVLIKSMAQHLIENSK VKLLRNQRFPASYHHAVETVVNMLMPHITQKFRDNPEASKNANHSLAVFIKRCFTFMDRGFVFKQINNYI SCFAPGDPKTLFEYKFEFLRVVCNHEHYIPLNLPMPFGKGRIQRYQDLQLDYSLTDEFCRNHFLVGLLLR EVGTALQEFREVRLIAISVLKNLLIKHSFDDRYASRSHQARIATLYLPLFGLLIENVQRINVRDVSPFPV NAGMTVKDESLALPAVNPLVTPQKGSTLDNSLHKDLLGAISGIASPYTTSTPNINSVRNADSRGSLISTD SGNSLPERNSEKSNSLDKHQQSSTLGNSVVRCDKLDQSEIKSLLMCFLYILKSMSDDALFTYWNKASTSE LMDFFTISEVCLHQFQYMGKRYIARTGMMHARLQQLGSLDNSLTFNHSYGHSDADVLHQSLLEANIATEV CLTALDTLSLFTLAFKNQLLADHGHNPLMKKVFDVYLCFLQKHQSETALKNVFTALRSLIYKFPSTFYEG RADMCAALCYEILKCCNSKLSSIRTEASQLLYFLMRNNFDYTGKKSFVRTHLQVIISVSQLIADVVGIGG TRFQQSLSIINNCANSDRLIKHTSFSSDVKDLTKRIRTVLMATAQMKEHENDPEMLVDLQYSLAKSYAST PELRKTWLDSMARIHVKNGDLSEAAMCYVHVTALVAEYLTRKEAVQWEPPLLPHSHSACLRRSRGGVFRQ GCTAFRVITPNIDEEASMMEDVGMQDVHFNEDVLMELLEQCADGLWKAERYELIADIYKLIIPIYEKRRD FERLAHLYDTLHRAYSKVTEVMHSGRRLLGTYFRVAFFGQAAQYQFTDSETDVEGFFEDEDGKEYIYKEP KLTPLSEISQRLLKLYSDKFGSENVKMIQDSGKVNPKDLDSKYAYIQVTHVIPFFDEKELQERKTEFERS HNIRRFMFEMPFTQTGKRQGGVEEQCKRRTILTAIHCFPYVKKRIPVMYQHHTDLNPIEVAIDEMSKKVA ELRQLCSSAEVDMIKLQLKLQGSVSVQVNAGPLAYARAFLDDTNTKRYPDNKVKLLKEVFRQFVEACGQA LAVNERLIKEDQLEYQEEMKANYREMAKELSEIMHEQLG SGPTRTRRLE - GFP Tag - V |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_015296 |
ORF Size | 6207 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_015296.1, NP_056111.1 |
RefSeq Size | 7522 bp |
RefSeq ORF | 6210 bp |
Locus ID | 23348 |
UniProt ID | Q9BZ29 |
Domains | PH |
Gene Summary | Guanine nucleotide-exchange factor (GEF) that activates CDC42 by exchanging bound GDP for free GTP. Overexpression induces filopodia formation.[UniProtKB/Swiss-Prot Function] |
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SDS |