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CDK5RAP2 (NM_018249) Human Tagged ORF Clone
CAT＃: RG214124
- TrueORF®
CDK5RAP2 (tGFP-tagged) - Human CDK5 regulatory subunit associated protein 2 (CDK5RAP2), transcript variant 1
ORF Plasmid: DDK
"NM_018249" in other vectors (2)
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CNY 20,810.00
货期*
8周
规格
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经常一起买 (5)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C48; Cep215; MCPH3 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG214124 representing NM_018249
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGACTTGGTGTTGGAAGAGGACGTCACCGTCCCTGGGACGCTCAGCGGCTGCAGTGGCCTTGTTC CCAGTGTACCAGATGACCTGGATGGCATCAACCCCAATGCTGGGTTGGGAAATGGTCTGCTCCCAAATGT GTCAGAAGAAACAGTGTCTCCCACCAGAGCACGGAACATGAAGGACTTTGAAAATCAAATCACTGAATTG AAGAAAGAAAACTTTAACCTAAAGCTCCGCATCTATTTCCTTGAGGAAAGAATGCAACAGGAATTTCATG GCCCCACTGAACATATCTACAAAACTAACATTGAGCTCAAGGTGGAAGTAGAAAGTCTGAAGCGGGAACT CCAGGAGAGAGAGCAGCTGCTCATCAAAGCCTCCAAAGCAGTTGAGAGCTTAGCTGAAGCAGGTGGCTCT GAAATCCAGCGGGTGAAAGAAGATGCTCGAAAGAAGGTGCAGCAGGTGGAAGATCTCCTAACTAAAAGAA TACTCCTTTTGGAAAAGGATGTGACAGCCGCCCAGGCAGAACTGGAAAAGGCCTTTGCAGGGACAGAGAC GGAGAAGGCTCTTCGGTTGCGTTTGGAAAGCAAGCTTTCAGAGATGAAGAAGATGCACGAGGGGGACTTG GCGATGGCTCTGGTCCTGGATGAGAAAGACAGACTGATTGAGGAGTTGAAGCTGTCTTTGAAGAGCAAAG AAGCTTTAATTCAGTGCCTTAAAGAGGAGAAATCTCAGATGGCATGTCCTGATGAGAATGTGTCATCTGG AGAGCTCCGAGGACTTTGTGCTGCTCCAAGGGAAGAAAAGGAGAGAGAAACTGAGGCTGCACAAATGGAG CATCAGAAGGAGAGAAACAGCTTTGAAGAGAGGATCCAGGCACTTGAAGAGGACCTGAGAGAGAAGGAAA GAGAAATTGCTACAGAGAAGAAAAATAGTCTAAAGAGGGATAAAGCCATTCAGGGTTTAACCATGGCATT AAAATCAAAGGAAAAAAAGGTTGAAGAACTTAACTCTGAAATTGAAAAGCTCAGTGCTGCCTTTGCTAAA GCCAGAGAGGCCCTACAGAAAGCACAGACCCAGGAATTTCAGGGGTCTGAAGACTATGAGACTGCTCTAT CAGGAAAGGAAGCCCTTTCGGCTGCGCTGCGCTCACAAAACCTCACCAAGAGTACAGAGAACCACAGACT GCGTAGAAGCATTAAGAAGATCACCCAGGAGCTGAGTGACTTGCAGCAGGAGAGGGAGAGACTGGAGAAG GACCTGGAGGAAGCCCATCGAGAGAAGAGCAAAGGAGACTGCACCATCCGTGATCTTAGAAATGAAGTTG AAAAATTACGCAATGAAGTGAATGAAAGAGAGAAAGCAATGGAAAATCGTTACAAGAGTCTTCTGAGTGA AAGCAATAAAAAATTGCACAATCAAGAGCAAGTGATCAAACATCTAACAGAAAGTACCAATCAGAAGGAC GTGTTGCTTCAGAAATTCAATGAAAAAGATTTGGAAGTAATACAGCAGAACTGCTATTTAATGGCTGCAG AGGATCTTGAGCTCAGGAGTGAAGGCTTAATAACAGAAAAGTGCTCTTCTCAACAGCCACCAGGCAGCAA AACCATCTTCTCTAAGGAAAAGAAACAATCATCAGACTATGAAGAGCTGATTCAGGTCTTAAAGAAAGAG CAGGACATCTATACCCATCTGGTCAAATCTCTGCAGGAATCAGACAGTATCAACAACCTGCAGGCTGAGT TAAACAAGATTTTTGCCCTGCGGAAGCAACTGGAGCAGGATGTGCTTTCATATCAGAATTTGCGGAAGAC CTTGGAGGAGCAGATCAGCGAAATTCGGAGGCGGGAAGAAGAATCATTTTCACTTTATAGTGATCAAACA TCTTATCTAAGTATTTGCCTTGAAGAAAACAATCGGTTTCAAGTGGAACATTTTTCTCAAGAAGAACTTA AGAAAAAGGTCAGTGACCTTATACAGCTAGTGAAGGAGCTGTATACAGACAACCAGCACCTGAAGAAAAC CATTTTTGATCTCTCCTGCATGGGTTTCCAGGGAAATGGGTTTCCAGATAGACTTGCGTCTACAGAACAA ACAGAGCTTCTGGCTAGCAAGGAGGACGAGGACACGATCAAAATTGGGGAGGATGACGAGATTAATTTCC TGAGTGACCAGCATTTGCAGCAGAGTAATGAGATTATGAAAGACCTTTCCAAAGGAGGCTGCAAAAATGG ATACTTAAGGCACACGGAGTCTAAGATTTCAGATTGTGATGGGGCCCACGCACCTGGCTGCCTAGAAGAA GGTGCATTCATAAACCTGCTTGCCCCTTTGTTCAATGAGAAGGCCACATTATTACTGGAATCCAGGCCAG ACCTTCTGAAAGTGGTACGGGAACTGCTTCTGGGACAACTATTCTTGACAGAGCAGGAAGTTTCTGGAGA ACACCTTGATGGTAAAACTGAGAAGACACCTAAGCAAAAAGGTGAACTTGTACATTTTGTCCAAACCAAC TCATTTTCCAAGCCACATGATGAACTGAAGTTGTCTTGTGAGGCCCAGCTAGTAAAGGCAGGCGAAGTGC CCAAGGTAGGACTGAAAGATGCCTCAGTGCAGACTGTGGCCACGGAGGGCGACCTGCTGAGATTCAAGCA TGAAGCAACAAGAGAGGCTTGGGAAGAGAAACCGATCAACACTGCACTCAGCGCAGAGCATCGGCCAGAG AACCTGCACGGGGTGCCTGGGTGGCAGGCTGCCCTCCTTTCCCTCCCTGGTATTACCAACAGAGAGGCTA AGAAGTCCCGCTTGCCAATCCTAATAAAACCATCCCGGTCATTAGGAAATATGTATCGTCTCCCTGCCAC CCAGGAGGTGGTGACGCAGCTGCAGAGCCAGATCTTGGAGCTGCAGGGGGAGCTGAAGGAGTTTAAAACT TGTAATAAGCAACTTCACCAAAAGTTAATTCTGGCTGAAGCAGTGATGGAGGGGAGGCCAACGCCCGACA AAACGTTGCTGAATGCTCAGCCCCCTGTGGGAGCAGCCTACCAGGACAGCCCAGGAGAGCAGAAAGGAAT TAAAACCACATCTTCTGTCTGGAGAGACAAGGAAATGGACAGTGATCAGCAAAGAAGCTACGAGATTGAC TCTGAGATTTGCCCACCTGATGACCTTGCCAGCTTGCCATCATGCAAAGAAAATCCTGAAGATGTTCTGA GCCCAACTTCAGTAGCTACTTACCTGAGTTCCAAGAGTCAGCCTTCTGCTAAAGTCAGTGTGATGGGGAC TGATCAGTCAGAGAGCATTAATACCTCAAATGAGACAGAATACTTAAAACAGAAAATCCATGACTTGGAA ACTGAGCTGGAAGGCTACCAGAATTTCATATTTCAGCTTCAAAAGCACTCCCAGTGCAGTGAGGCCATAA TTACAGTTTTGTGTGGGACAGAAGGGGCCCAGGATGGCTTGAGCAAGCCCAAGAATGGTTCTGATGGGGA AGAAATGACCTTTTCAAGTTTGCACCAAGTGCGATACGTGAAACACGTGAAAATCCTCGGTCCGCTGGCC CCAGAGATGATTGACAGCAGGGTGCTGGAGAACCTCAAACAGCAGCTGGAGGAACAGGAATACAAGCTGC AGAAGGAGCAGAATTTGAACATGCAACTTTTCAGTGAGATCCATAATCTGCAGAATAAGTTCAGAGATCT CTCACCTCCCAGATACGATTCATTAGTTCAGTCCCAAGCCAGGGAGCTCTCCCTTCAACGGCAGCAGATT AAGGATGGCCATGGCATCTGTGTCATCTCCCGTCAACACATGAACACCATGATTAAGGCATTTGAGGAGT TGCTGCAGGCCAGTGATGTGGATTACTGTGTGGCCGAGGGTTTCCAGGAACAGCTGAATCAATGTGCTGA GCTGCTGGAGAAATTGGAAAAGCTATTTCTCAACGGAAAATCAGTTGGAGTGGAAATGAACACCCAGAAT GAACTGATGGAGAGGATTGAGGAAGACAACTTAACCTACCAACATCTTCTGCCTGAATCTCCTGAGCCTT CAGCCTCTCATGCGCTCTCTGATTATGAAACATCTGAAAAGTCCTTCTTCTCACGAGACCAGAAGCAAGA TAATGAGACAGAGAAGACTTCAGTTATGGTGAACAGTTTTTCTCAAGACTTACTAATGGAACACATACAG GAAATTCGAACTTTGAGAAAGCGTTTAGAAGAATCTATTAAAACAAATGAGAAGCTACGGAAACAGTTGG AACGGCAAGGATCTGAATTTGTTCAAGGTTCTACAAGCATTTTTGCTTCTGGTTCAGAGCTTCATAGTTC TCTAACATCAGAAATTCATTTCTTGAGGAAGCAGAACCAGGCCCTCAATGCAATGCTCATTAAAGGATCC AGAGATAAACAGAAGGAGAATGACAAATTACGAGAGTCCCTCTCCAGGAAGACCGTGAGCCTGGAGCACC TTCAGCGGGAGTATGCCAGCGTGAAGGAAGAAAATGAAAGGCTGCAGAAAGAAGGCAGCGAGAAGGAGAG ACACAACCAGCAGCTGATCCAGGAGGTCCGCTGCAGCGGCCAGGAGCTGAGCAGGGTGCAGGAGGAGGTG AAGTTGAGGCAGCAGCTGCTCTCACAGAATGACAAGCTATTGCAGTCTCTCCGAGTGGAGCTGAAGGCGT ATGAGAAGCTGGATGAAGAGCACAGGAGACTGAGAGAGGCGTCGGGAGAAGGCTGGAAGGGGCAGGATCC TTTCAGGGACCTGCACAGCCTCCTGATGGAGATCCAGGCTCTGCGCTTGCAACTAGAAAGGAGCATCGAA ACCAGCAGCACTCTGCAGAGCAGGCTCAAGGAACAGCTGGCAAGGGGGGCAGAGAAGGCACAGGAAGGAG CCCTCACTCTGGCTGTCCAAGCCGTGTCCATCCCTGAGGTGCCCCTTCAGCCTGACAAACACGATGGTGA CAAATATCCCATGGAAAGTGATAATTCATTTGATCTGTTTGATTCCTCCCAGGCAGTGACACCAAAATCA GTTTCAGAGACTCCTCCACTCTCTGGGAATGACACGGACTCCCTCTCCTGCGACAGTGGCAGTTCGGCAA CTAGCACTCCGTGTGTGTCCCGCCTGGTCACTGGCCACCACCTGTGGGCCAGCAAGAATGGCCGCCATGT CCTGGGCCTGATTGAGGACTATGAGGCCCTGCTCAAACAGATCAGCCAGGGACAGAGGCTCCTTGCTGAA ATGGACATTCAAACCCAAGAGGCTCCCAGCTCCACAAGTCAAGAGCTGGGAACAAAGGGTCCACACCCAG CACCACTGAGCAAGTTTGTGAGCAGTGTGAGCACGGCCAAGCTGACCCTGGAAGAGGCCTACAGGCGGCT GAAGCTTCTCTGGAGAGTCTCACTCCCCGAGGATGGCCAGTGCCCCCTTCACTGTGAGCAGATTGGAGAA ATGAAGGCAGAGGTCACCAAACTACATAAAAAATTGTTTGAACAAGAAAAGAAGTTGCAAAACACCATGA AGCTTTTGCAGCTGAGCAAGCGCCAGGAAAAAGTCATCTTTGATCAATTGGTCGTAACCCACAAAATCCT TCGGAAGGCCAGAGGAAACCTGGAGCTTAGGCCTGGGGGAGCCCATCCAGGAACATGCAGTCCCAGCAGA CCAGGCTCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG214124 representing NM_018249
Red=Cloning site Green=Tags(s) MMDLVLEEDVTVPGTLSGCSGLVPSVPDDLDGINPNAGLGNGLLPNVSEETVSPTRARNMKDFENQITEL KKENFNLKLRIYFLEERMQQEFHGPTEHIYKTNIELKVEVESLKRELQEREQLLIKASKAVESLAEAGGS EIQRVKEDARKKVQQVEDLLTKRILLLEKDVTAAQAELEKAFAGTETEKALRLRLESKLSEMKKMHEGDL AMALVLDEKDRLIEELKLSLKSKEALIQCLKEEKSQMACPDENVSSGELRGLCAAPREEKERETEAAQME HQKERNSFEERIQALEEDLREKEREIATEKKNSLKRDKAIQGLTMALKSKEKKVEELNSEIEKLSAAFAK AREALQKAQTQEFQGSEDYETALSGKEALSAALRSQNLTKSTENHRLRRSIKKITQELSDLQQERERLEK DLEEAHREKSKGDCTIRDLRNEVEKLRNEVNEREKAMENRYKSLLSESNKKLHNQEQVIKHLTESTNQKD VLLQKFNEKDLEVIQQNCYLMAAEDLELRSEGLITEKCSSQQPPGSKTIFSKEKKQSSDYEELIQVLKKE QDIYTHLVKSLQESDSINNLQAELNKIFALRKQLEQDVLSYQNLRKTLEEQISEIRRREEESFSLYSDQT SYLSICLEENNRFQVEHFSQEELKKKVSDLIQLVKELYTDNQHLKKTIFDLSCMGFQGNGFPDRLASTEQ TELLASKEDEDTIKIGEDDEINFLSDQHLQQSNEIMKDLSKGGCKNGYLRHTESKISDCDGAHAPGCLEE GAFINLLAPLFNEKATLLLESRPDLLKVVRELLLGQLFLTEQEVSGEHLDGKTEKTPKQKGELVHFVQTN SFSKPHDELKLSCEAQLVKAGEVPKVGLKDASVQTVATEGDLLRFKHEATREAWEEKPINTALSAEHRPE NLHGVPGWQAALLSLPGITNREAKKSRLPILIKPSRSLGNMYRLPATQEVVTQLQSQILELQGELKEFKT CNKQLHQKLILAEAVMEGRPTPDKTLLNAQPPVGAAYQDSPGEQKGIKTTSSVWRDKEMDSDQQRSYEID SEICPPDDLASLPSCKENPEDVLSPTSVATYLSSKSQPSAKVSVMGTDQSESINTSNETEYLKQKIHDLE TELEGYQNFIFQLQKHSQCSEAIITVLCGTEGAQDGLSKPKNGSDGEEMTFSSLHQVRYVKHVKILGPLA PEMIDSRVLENLKQQLEEQEYKLQKEQNLNMQLFSEIHNLQNKFRDLSPPRYDSLVQSQARELSLQRQQI KDGHGICVISRQHMNTMIKAFEELLQASDVDYCVAEGFQEQLNQCAELLEKLEKLFLNGKSVGVEMNTQN ELMERIEEDNLTYQHLLPESPEPSASHALSDYETSEKSFFSRDQKQDNETEKTSVMVNSFSQDLLMEHIQ EIRTLRKRLEESIKTNEKLRKQLERQGSEFVQGSTSIFASGSELHSSLTSEIHFLRKQNQALNAMLIKGS RDKQKENDKLRESLSRKTVSLEHLQREYASVKEENERLQKEGSEKERHNQQLIQEVRCSGQELSRVQEEV KLRQQLLSQNDKLLQSLRVELKAYEKLDEEHRRLREASGEGWKGQDPFRDLHSLLMEIQALRLQLERSIE TSSTLQSRLKEQLARGAEKAQEGALTLAVQAVSIPEVPLQPDKHDGDKYPMESDNSFDLFDSSQAVTPKS VSETPPLSGNDTDSLSCDSGSSATSTPCVSRLVTGHHLWASKNGRHVLGLIEDYEALLKQISQGQRLLAE MDIQTQEAPSSTSQELGTKGPHPAPLSKFVSSVSTAKLTLEEAYRRLKLLWRVSLPEDGQCPLHCEQIGE MKAEVTKLHKKLFEQEKKLQNTMKLLQLSKRQEKVIFDQLVVTHKILRKARGNLELRPGGAHPGTCSPSR PGS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018249 |
| ORF Size | 5679 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018249.6 |
| RefSeq Size | 6230 bp |
| RefSeq ORF | 5682 bp |
| Locus ID | 55755 |
| UniProt ID | Q96SN8 |
| Gene Summary | This gene encodes a regulator of CDK5 (cyclin-dependent kinase 5) activity. The protein encoded by this gene is localized to the centrosome and Golgi complex, interacts with CDK5R1 and pericentrin (PCNT), plays a role in centriole engagement and microtubule nucleation, and has been linked to primary microcephaly and Alzheimer's disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013] |
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