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Cyclin T2 (CCNT2) (NM_058241) Human Tagged ORF Clone
CAT＃: RG214170
- TrueORF®
CCNT2 (tGFP-tagged) - Human cyclin T2 (CCNT2), transcript variant b
ORF Plasmid: DDK
"NM_058241" in other vectors (4)
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CNY 6,370.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CYCT2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG214170 representing NM_058241
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCGGGCCGTGGAGCTTCTTCTCGCTGGTTCTTTACTCGGGAACAGCTGGAGAACACGCCGAGCC GCCGCTGCGGAGTGGAGGCGGATAAAGAGCTCTCGTGCCGCCAGCAGGCGGCCAACCTCATCCAGGAGAT GGGACAGCGTCTCAATGTCTCTCAGCTTACAATAAACACTGCGATTGTTTATATGCACAGGTTTTATATG CACCATTCTTTCACCAAATTCAACAAAAATATAATATCGTCTACTGCATTATTTTTGGCTGCAAAAGTGG AAGAACAGGCTCGAAAACTTGAACATGTTATCAAAGTAGCACATGCTTGTCTTCATCCTCTAGAGCCACT GCTGGATACTAAATGTGATGCTTACCTTCAACAGACTCAAGAACTGGTTATACTTGAAACCATAATGCTA CAAACTCTAGGTTTTGAGATCACCATTGAACACCCACACACAGATGTGGTGAAATGTACCCAGTTAGTAA GAGCAAGCAAGGATTTGGCACAGACATCCTATTTCATGGCTACCAACAGTCTGCATCTTACAACCTTCTG TCTTCAGTACAAACCAACAGTGATAGCATGTGTATGCATTCATTTGGCTTGCAAATGGTCCAATTGGGAG ATCCCTGTATCAACTGATGGAAAGCATTGGTGGGAATATGTGGATCCTACAGTTACTCTAGAATTATTAG ATGAGCTAACACATGAGTTTCTACAAATATTGGAGAAAACGCCTAATAGGTTGAAGAAGATTCGAAACTG GAGGGCTAATCAGGCAGCTAGGAAACCAAAAGTAGATGGACAGGTATCAGAGACACCACTTCTTGGTTCA TCTTTGGTCCAGAATTCCATTTTAGTAGATAGTGTCACTGGTGTGCCTACAAACCCAAGTTTTCAGAAAC CATCTACATCAGCATTCCCTGCGCCAGTACCTCTAAATTCAGGAAATATTTCTGTTCAAGACAGCCATAC ATCTGATAATTTGTCAATGCTAGCAACAGGAATGCCAAGTACTTCATACGGTTTATCATCACACCAGGAA TGGCCTCAACATCAAGACTCAGCAAGGACAGAACAGCTATATTCACAGAAACAGGAGACATCTTTGTCTG GTAGCCAGTACAACATCAACTTCCAGCAGGGACCTTCTATATCACTGCATTCAGGATTACATCACAGACC TGACAAAATTTCAGATCATTCTTCTGTTAAGCAAGAATATACTCATAAAGCAGGGAGCAGTAAACACCAT GGGCCAATTTCCACTACTCCAGGAATAATTCCTCAGAAAATGTCTTTAGATAAATATAGAGAAAAGCGTA AACTAGAAACTCTTGATCTCGATGTAAGGGATCATTATATAGCTGCCCAGGTAGAACAGCAGCACAAACA AGGGCAGTCACAGGCAGCCAGCAGCAGTTCTGTTACTTCTCCCATTAAAATGAAAATACCTATCGCAAAT ACTGAAAAATACATGGCAGACAAAAAGGAAAAGAGTGGGTCACTGAAATTACGGATTCCAATACCACCCA CTGATAAAAGCGCCAGTAAAGAAGAACTGAAAATGAAAATAAAAGTTTCTTCTTCAGAAAGACACAGCTC TTCTGATGAAGGCAGTGGGAAGAGCAAACATTCAAGCCCACATATTAGCAGAGACCATAAGGAGAAGCAC AAGGAGCATCCTTCAAGCCGCCACCACACCAGCAGCCACAAGCATTCCCACTCGCATAGTGGCAGCAGCA GCGGTGGCAGTAAACACAGTGCCGACGGAATACCACCCACTGTTCTGAGGAGTCCTGTTGGCCTGAGCAG TGATGGCATTTCCTCTAGCTCCAGCTCTTCAAGGAAGAGGCTGCATGTCAATGATGCATCTCACAACCAC CACTCCAAAATGAGCAAAAGTTCCAAAAGTTCAGGTAGTTCATCTAGTTCTTCCTCCTCTGTTAAGCAGT ATATATCCTCTCACAACTCTGTTTTTAACCATCCCTTACCCCCTCCTCCCCCTGTCACATACCAGGTGGG CTACGGACATCTCAGCACCCTCGTGAAACTGGACAAGAAGCCAGTGGAGACCAACGGTCCTGATGCCAAT CACGAGTACAGTACAAGCAGCCAGCATATGGACTACAAAGACACATTCGACATGCTGGACTCACTGTTAA GTGCCCAAGGAATGAACATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG214170 representing NM_058241
Red=Cloning site Green=Tags(s) MASGRGASSRWFFTREQLENTPSRRCGVEADKELSCRQQAANLIQEMGQRLNVSQLTINTAIVYMHRFYM HHSFTKFNKNIISSTALFLAAKVEEQARKLEHVIKVAHACLHPLEPLLDTKCDAYLQQTQELVILETIML QTLGFEITIEHPHTDVVKCTQLVRASKDLAQTSYFMATNSLHLTTFCLQYKPTVIACVCIHLACKWSNWE IPVSTDGKHWWEYVDPTVTLELLDELTHEFLQILEKTPNRLKKIRNWRANQAARKPKVDGQVSETPLLGS SLVQNSILVDSVTGVPTNPSFQKPSTSAFPAPVPLNSGNISVQDSHTSDNLSMLATGMPSTSYGLSSHQE WPQHQDSARTEQLYSQKQETSLSGSQYNINFQQGPSISLHSGLHHRPDKISDHSSVKQEYTHKAGSSKHH GPISTTPGIIPQKMSLDKYREKRKLETLDLDVRDHYIAAQVEQQHKQGQSQAASSSSVTSPIKMKIPIAN TEKYMADKKEKSGSLKLRIPIPPTDKSASKEELKMKIKVSSSERHSSSDEGSGKSKHSSPHISRDHKEKH KEHPSSRHHTSSHKHSHSHSGSSSGGSKHSADGIPPTVLRSPVGLSSDGISSSSSSSRKRLHVNDASHNH HSKMSKSSKSSGSSSSSSSSVKQYISSHNSVFNHPLPPPPPVTYQVGYGHLSTLVKLDKKPVETNGPDAN HEYSTSSQHMDYKDTFDMLDSLLSAQGMNM TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_058241 |
| ORF Size | 2190 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_058241.3 |
| RefSeq Size | 6923 bp |
| RefSeq ORF | 2193 bp |
| Locus ID | 905 |
| UniProt ID | O60583 |
| Domains | CYCLIN, cyclin |
| Protein Families | Druggable Genome, Transcription Factors |
| Gene Summary | The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin and its kinase partner CDK9 were found to be subunits of the transcription elongation factor p-TEFb. The p-TEFb complex containing this cyclin was reported to interact with, and act as a negative regulator of human immunodeficiency virus type 1 (HIV-1) Tat protein. A pseudogene of this gene is found on chromosome 1. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Dec 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214170 | CCNT2 (Myc-DDK-tagged)-Human cyclin T2 (CCNT2), transcript variant b |
CNY 5,800.00 |
|
| RC214170L3 | Lenti-ORF clone of CCNT2 (Myc-DDK-tagged)-Human cyclin T2 (CCNT2), transcript variant b |
CNY 7,700.00 |
|
| RC214170L4 | Lenti-ORF clone of CCNT2 (mGFP-tagged)-Human cyclin T2 (CCNT2), transcript variant b |
CNY 7,700.00 |
|
| SC314566 | CCNT2 (untagged)-Human cyclin T2 (CCNT2), transcript variant b |
CNY 14,060.00 |
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