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GPR115 (ADGRF4) (NM_153838) Human Tagged ORF Clone
CAT＃: RG214357
- TrueORF®
GPR115 (tGFP-tagged) - Human G protein-coupled receptor 115 (GPR115)
ORF Plasmid: DDK
"NM_153838" in other vectors (6)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | GPR115; PGR18 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG214357 representing NM_153838
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAATGAAGTCCCAGGCAACCATGATTTGCTGCTTAGTGTTCTTTCTGTCCACAGAATGTTCCCACT ATAGATCCAAGATTCACCTAAAAGCTGGAGATAAACTTCAAAGCCCTGAAGGGAAACCCAAGACTGGAAG GATCCAAGAGAAATGCGAAGGACCTTGTATTTCTTCTTCCAACTGCAGCCAGCCCTGTGCTAAGGACTTT CATGGAGAAATAGGATTTACATGTAATCAAAAAAAGTGGCAAAAATCAGCTGAAACATGTACAAGCCTTT CTGTGGAAAAACTCTTTAAGGACTCAACTGGTGCATCTCGCCTTTCTGTAGCAGCACCATCTATACCTCT GCATATTCTAGACTTTCGAGCTCCAGAGACCATTGAGAGTGTAGCTCAAGGAATCCGTAAGAACTGCCCC TTTGATTATGCCTGCATCACTGACATGGTGAAATCATCAGAAACAACATCTGGAAATATTGCATTTATAG TGGAGTTATTAAAAAATATTTCTACAGACTTGTCTGATAATGTTACTCGAGAGAAAATGAAGAGCTATAG TGAAGTGGCCAACCACATCCTCGACACAGCAGCCATTTCAAACTGGGCTTTCATTCCCAACAAAAATGCC AGCTCGGATTTGTTGCAGTCAGTGAATTTGTTTGCCAGACAACTCCACATCCACAATAATTCTGAGAACA TTGTGAATGAACTCTTCATTCAGACAAAAGGGTTTCACATCAACCATAATACCTCAGAGAAAAGCCTCAA TTTCTCCATGAGCATGAACAATACCACAGAAGATATCTTAGGAATGGTACAGATTCCCAGGCAAGAGCTA AGGAAGCTGTGGCCAAATGCATCCCAAGCCATTAGCATAGCTTTCCCAACCTTGGGGGCTATCCTGAGAG AAGCCCACTTGCAAAATGTGAGTCTTCCCAGACAGGTAAATGGTCTGGTGCTATCAGTGGTTTTACCAGA AAGGTTGCAAGAAATCATACTCACCTTCGAAAAGATCAATAAAACCCGCAATGCCAGAGCCCAGTGTGTT GGCTGGCACTCCAAGAAAAGGAGATGGGATGAGAAAGCGTGCCAAATGATGTTGGATATCAGGAACGAAG TGAAATGCCGCTGTAACTACACCAGTGTGGTGATGTCTTTTTCCATTCTCATGTCCTCCAAATCGATGAC CGACAAAGTTCTGGACTACATCACCTGCATTGGGCTCAGCGTCTCAATCCTAAGCTTGGTTCTTTGCCTG ATCATTGAAGCCACAGTGTGGTCCCGGGTGGTTGTGACGGAGATATCATACATGCGTCACGTGTGCATCG TGAATATAGCAGTGTCCCTTCTGACTGCCAATGTGTGGTTTATCATAGGCTCTCACTTTAACATTAAGGC CCAGGACTACAACATGTGTGTTGCAGTGACATTTTTCAGCCACTTTTTCTACCTCTCTCTGTTTTTCTGG ATGCTCTTCAAAGCATTGCTCATCATTTATGGAATATTGGTCATTTTCCGTAGGATGATGAAGTCCCGAA TGATGGTCATTGGCTTTGCCATTGGCTATGGGTGCCCATTGATCATTGCTGTCACTACAGTTGCTATCAC AGAGCCAGAGAACGGCTACATGAGACCTGAGGCCTGTTGGCTTAACTGGGACAATACCAAAGCCCTTTTA GCATTTGCCATCCCGGCGTTCGTCATTGTGGCTGTAAATCTGATTGTGGTTTTGGTTGTTGCTGTCAACA CTCAGAGGCCCTCTATTGGCAGTTCCAAGTCTCAGGATGTGGTCATAATTATGAGGATCAGCAAAAATGT TGCCATCCTCACTCCACTGCTGGGACTGACCTGGGGTTTTGGAATAGCCACTCTCATAGAAGGCACTTCC TTGACGTTCCATATAATTTTTGCCTTGCTCAATGCTTTCCAGGGTTTTTTCATCCTGCTGTTTGGAACCA TTATGGATCACAAGATAAGAGATGCTTTGAGGATGAGGATGTCTTCACTGAAGGGGAAATCGAGGGCAGC TGAGAATGCATCACTAGGCCCAACCAATGGATCTAAATTAATGAATCGTCAAGGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG214357 representing NM_153838
Red=Cloning site Green=Tags(s) MKMKSQATMICCLVFFLSTECSHYRSKIHLKAGDKLQSPEGKPKTGRIQEKCEGPCISSSNCSQPCAKDF HGEIGFTCNQKKWQKSAETCTSLSVEKLFKDSTGASRLSVAAPSIPLHILDFRAPETIESVAQGIRKNCP FDYACITDMVKSSETTSGNIAFIVELLKNISTDLSDNVTREKMKSYSEVANHILDTAAISNWAFIPNKNA SSDLLQSVNLFARQLHIHNNSENIVNELFIQTKGFHINHNTSEKSLNFSMSMNNTTEDILGMVQIPRQEL RKLWPNASQAISIAFPTLGAILREAHLQNVSLPRQVNGLVLSVVLPERLQEIILTFEKINKTRNARAQCV GWHSKKRRWDEKACQMMLDIRNEVKCRCNYTSVVMSFSILMSSKSMTDKVLDYITCIGLSVSILSLVLCL IIEATVWSRVVVTEISYMRHVCIVNIAVSLLTANVWFIIGSHFNIKAQDYNMCVAVTFFSHFFYLSLFFW MLFKALLIIYGILVIFRRMMKSRMMVIGFAIGYGCPLIIAVTTVAITEPENGYMRPEACWLNWDNTKALL AFAIPAFVIVAVNLIVVLVVAVNTQRPSIGSSKSQDVVIIMRISKNVAILTPLLGLTWGFGIATLIEGTS LTFHIIFALLNAFQGFFILLFGTIMDHKIRDALRMRMSSLKGKSRAAENASLGPTNGSKLMNRQG TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_153838 |
| ORF Size | 2085 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_153838.2, NP_722580.2 |
| RefSeq Size | 2574 bp |
| RefSeq ORF | 2088 bp |
| Locus ID | 221393 |
| UniProt ID | Q8IZF3 |
| Protein Families | Druggable Genome, Transmembrane |
| Gene Summary | Sequence analysis of this gene suggests that it is encodes a member of the superfamily of G protein-couple receptors. G protein-coupled receptors typically contain seven hydrophobic transmembrane domains, interact with guanine nucleotide binding regulatory proteins, and detect molecules outside the cell and act to transduce these signals into intracellular responses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214357 | GPR115 (Myc-DDK-tagged)-Human G protein-coupled receptor 115 (GPR115) |
CNY 7,632.00 |
|
| RC214357L1 | Lenti-ORF clone of GPR115 (Myc-DDK-tagged)-Human G protein-coupled receptor 115 (GPR115) |
CNY 10,032.00 |
|
| RC214357L2 | Lenti-ORF clone of GPR115 (mGFP-tagged)-Human G protein-coupled receptor 115 (GPR115) |
CNY 7,700.00 |
|
| RC214357L3 | Lenti-ORF clone of GPR115 (Myc-DDK-tagged)-Human G protein-coupled receptor 115 (GPR115) |
CNY 7,700.00 |
|
| RC214357L4 | Lenti-ORF clone of GPR115 (mGFP-tagged)-Human G protein-coupled receptor 115 (GPR115) |
CNY 7,700.00 |
|
| SC306668 | GPR115 (untagged)-Human G protein-coupled receptor 115 (GPR115) |
CNY 5,096.00 |
