GNRHR (NM_000406) Human Tagged ORF Clone
CAT#: RG214403
- TrueORF®
GNRHR (tGFP-tagged) - Human gonadotropin-releasing hormone receptor (GNRHR), transcript variant 1
ORF Plasmid: DDK
"NM_000406" in other vectors (6)
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CNY 4,000.00
CNY 4,370.00
Cited in 1 publication. |
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | GNRHR1; GRHR; HH7; LHRHR; LRHR |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG214403 representing NM_000406
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAACAGTGCCTCTCCTGAACAGAATCAAAATCACTGTTCAGCCATCAACAACAGCATCCCACTGA TGCAGGGCAACCTCCCCACTCTGACCTTGTCTGGAAAGATCCGAGTGACGGTTACTTTCTTCCTTTTTCT GCTCTCTGCGACCTTTAATGCTTCTTTCTTGTTGAAACTTCAGAAGTGGACACAGAAGAAAGAGAAAGGG AAAAAGCTCTCAAGAATGAAGCTGCTCTTAAAACATCTGACCTTAGCCAACCTGTTGGAGACTCTGATTG TCATGCCACTGGATGGGATGTGGAACATTACAGTCCAATGGTATGCTGGAGAGTTACTCTGCAAAGTTCT CAGTTATCTAAAGCTTTTCTCCATGTATGCCCCAGCCTTCATGATGGTGGTGATCAGCCTGGACCGCTCC CTGGCTATCACGAGGCCCCTAGCTTTGAAAAGCAACAGCAAAGTCGGACAGTCCATGGTTGGCCTGGCCT GGATCCTCAGTAGTGTCTTTGCAGGACCACAGTTATACATCTTCAGGATGATTCATCTAGCAGACAGCTC TGGACAGACAAAAGTTTTCTCTCAATGTGTAACACACTGCAGTTTTTCACAATGGTGGCATCAAGCATTT TATAACTTTTTCACCTTCAGCTGCCTCTTCATCATCCCTCTTTTCATCATGCTGATCTGCAATGCAAAAA TCATCTTCACCCTGACACGGGTCCTTCATCAGGACCCCCACGAACTACAACTGAATCAGTCCAAGAACAA TATACCAAGAGCACGGCTGAAGACTCTAAAAATGACGGTTGCATTTGCCACTTCATTTACTGTCTGCTGG ACTCCCTACTATGTCCTAGGAATTTGGTATTGGTTTGATCCTGAAATGTTAAACAGGTTGTCAGACCCAG TAAATCACTTCTTCTTTCTCTTTGCCTTTTTAAACCCATGCTTTGATCCACTTATCTATGGATATTTTTC TCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG214403 representing NM_000406
Red=Cloning site Green=Tags(s) MANSASPEQNQNHCSAINNSIPLMQGNLPTLTLSGKIRVTVTFFLFLLSATFNASFLLKLQKWTQKKEKG KKLSRMKLLLKHLTLANLLETLIVMPLDGMWNITVQWYAGELLCKVLSYLKLFSMYAPAFMMVVISLDRS LAITRPLALKSNSKVGQSMVGLAWILSSVFAGPQLYIFRMIHLADSSGQTKVFSQCVTHCSFSQWWHQAF YNFFTFSCLFIIPLFIMLICNAKIIFTLTRVLHQDPHELQLNQSKNNIPRARLKTLKMTVAFATSFTVCW TPYYVLGIWYWFDPEMLNRLSDPVNHFFFLFAFLNPCFDPLIYGYFSL TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_000406 |
ORF Size | 984 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000406.3 |
RefSeq Size | 5843 bp |
RefSeq ORF | 987 bp |
Locus ID | 2798 |
UniProt ID | P30968 |
Protein Families | Druggable Genome, GPCR, Transmembrane |
Protein Pathways | GnRH signaling pathway, Neuroactive ligand-receptor interaction |
Gene Summary | This gene encodes the receptor for type 1 gonadotropin-releasing hormone. This receptor is a member of the seven-transmembrane, G-protein coupled receptor (GPCR) family. It is expressed on the surface of pituitary gonadotrope cells as well as lymphocytes, breast, ovary, and prostate. Following binding of gonadotropin-releasing hormone, the receptor associates with G-proteins that activate a phosphatidylinositol-calcium second messenger system. Activation of the receptor ultimately causes the release of gonadotropic luteinizing hormone (LH) and follicle stimulating hormone (FSH). Defects in this gene are a cause of hypogonadotropic hypogonadism (HH). Alternative splicing results in multiple transcript variants encoding different isoforms. More than 18 transcription initiation sites in the 5' region and multiple polyA signals in the 3' region have been identified for this gene. [provided by RefSeq, Jul 2008] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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A novel GNRHR gene mutation causing Congenital Hypogonadotropic Hypogonadism (CHH) in a Brazilian Kindred
,Correa-Silva, SR;da Silva Fausto, J;Kizys, MML;Filipelli, R;Marco Antonio, DS;Oku, AY;Furuzawa, GK;Orchard, EVH;Costa-Barbosa, FA;Mitne-Neto, M;Dias-da-Silva, MR;,
J. Neuroendocrinol.
,PubMed ID 30415482
[GNRHR]
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Documents
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FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC214403 | GNRHR (Myc-DDK-tagged)-Human gonadotropin-releasing hormone receptor (GNRHR), transcript variant 1 |
CNY 2,400.00 |
|
RC214403L1 | Lenti ORF clone of Human gonadotropin-releasing hormone receptor (GNRHR), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
RC214403L2 | Lenti ORF clone of Human gonadotropin-releasing hormone receptor (GNRHR), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC214403L3 | Lenti ORF clone of Human gonadotropin-releasing hormone receptor (GNRHR), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
RC214403L4 | Lenti ORF clone of Human gonadotropin-releasing hormone receptor (GNRHR), transcript variant 1, mGFP tagged |
CNY 4,800.00 |
|
SC316990 | GNRHR (untagged)-Human gonadotropin-releasing hormone receptor (GNRHR), transcript variant 1 |
CNY 2,400.00 |