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GPR158 (NM_020752) Human Tagged ORF Clone
CAT＃: RG214433
- TrueORF®
GPR158 (tGFP-tagged) - Human G protein-coupled receptor 158 (GPR158)
ORF Plasmid: DDK
"NM_020752" in other vectors (5)
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CNY 13,400.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG214433 representing NM_020752
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGCCATGGCTTACCCCTTACTCCTCTGCCTCCTGCTTGCTCAGCTGGGATTGGGAGCTGTTGGCG CCAGCCGCGACCCCCAAGGACGGCCGGATTCCCCTCGAGAGAGGACCCCGAAGGGGAAGCCGCACGCCCA GCAGCCGGGTCGAGCCTCTGCCTCGGACTCCTCGGCTCCCTGGAGCCGCTCCACCGATGGCACCATCTTG GCGCAGAAACTCGCCGAGGAGGTGCCCATGGACGTGGCCTCTTACCTCTACACCGGGGACTCCCACCAGC TGAAGCGAGCCAACTGCTCCGGCCGCTACGAGTTGGCGGGCCTGCCGGGGAAGTGGCCAGCCCTGGCCAG CGCGCACCCCTCCTTGCACCGGGCGCTGGACACACTGACACACGCCACCAACTTCCTCAACGTGATGCTG CAGAGCAATAAGTCGCGGGAGCAGAACTTGCAGGACGACCTGGATTGGTACCAGGCGCTGGTGTGGAGCC TTCTGGAGGGCGAGCCCAGCATCTCCCGGGCGGCCATCACCTTCAGCACCGATTCGCTGTCCGCACCGGC CCCACAGGTCTTCCTCCAGGCCACGCGCGAGGAGAGCCGCATCCTGCTCCAAGACCTGTCCTCCTCCGCA CCCCACCTGGCCAACGCCACTCTGGAGACCGAGTGGTTCCACGGCCTCCGGCGCAAGTGGAGGCCCCACT TACACCGCCGCGGCCCCAATCAGGGGCCCCGGGGCCTGGGCCACAGCTGGCGGCGCAAGGACGGGCTCGG CGGGGACAAGAGCCACTTCAAGTGGTCTCCGCCTTATCTGGAGTGCGAGAACGGGAGTTACAAGCCCGGG TGGCTGGTTACTCTTTCCTCTGCCATCTACGGGTTGCAGCCTAACCTGGTCCCGGAATTCAGGGGTGTCA TGAAAGTTGACATAAATCTTCAGAAAGTGGACATTGACCAATGCTCAAGTGATGGCTGGTTTTCAGGAAC TCATAAATGCCACCTCAACAATTCAGAGTGTATGCCAATTAAAGGCCTAGGATTCGTTCTTGGAGCCTAT GAGTGCATTTGCAAAGCAGGATTCTATCATCCTGGAGTCTTACCAGTGAACAACTTTCGGAGAAGGGGTC CGGATCAGCATATTTCAGGAAGTACAAAAGATGTGTCAGAAGAAGCCTATGTCTGCCTACCTTGCAGGGA GGGCTGCCCCTTCTGTGCTGATGACAGCCCATGCTTCGTCCAGGAAGATAAGTATTTACGACTTGCCATC ATCTCCTTCCAAGCCCTGTGTATGCTGCTCGACTTCGTTAGCATGCTGGTGGTCTACCACTTTCGCAAAG CAAAGAGCATCCGGGCATCGGGCCTTATCCTGTTGGAAACGATCCTTTTTGGATCTCTGCTCCTATACTT TCCAGTTGTTATTTTGTACTTTGAGCCAAGCACATTTCGCTGTATTCTCCTAAGATGGGCTCGTCTTCTC GGTTTTGCTACTGTTTACGGAACTGTCACTCTCAAACTTCACAGGGTTTTGAAGGTGTTTCTTTCACGAA CGGCTCAACGAATTCCATATATGACTGGCGGACGGGTCATGAGGATGCTGGCAGTAATACTCTTGGTAGT GTTTTGGTTTCTCATTGGCTGGACTTCATCTGTGTGCCAGAATTTGGAGAAACAGATTTCACTTATTGGC CAGGGGAAAACATCCGATCACCTCATCTTCAATATGTGCCTCATTGACCGCTGGGACTACATGACAGCAG TTGCTGAATTTTTATTCCTCTTGTGGGGTGTTTATCTCTGCTATGCAGTGCGGACAGTCCCATCGGCATT CCATGAGCCCCGCTATATGGCTGTTGCAGTTCACAATGAGCTCATCATCTCTGCTATATTCCATACAATT AGATTTGTTCTTGCCTCAAGACTTCAGTCTGATTGGATGTTGATGCTGTATTTTGCACATACTCATTTGA CTGTGACAGTCACCATTGGGTTGCTTTTGATTCCAAAGTTTTCACATTCAAGCAATAACCCACGAGATGA TATTGCTACAGAAGCATATGAGGATGAGCTAGACATGGGCCGATCTGGATCCTACCTGAACAGCAGTATC AATTCAGCCTGGAGTGAGCACAGCTTGGATCCAGAGGACATTCGGGACGAGCTGAAAAAACTCTATGCCC AACTGGAAATATATAAAAGAAAGAAGATGATCACAAACAACCCCCACCTCCAGAAAAAGCGGTGCTCGAA GAAGGGCCTAGGTCGTTCCATCATGAGACGCATTACGGAGATCCCAGAGACAGTCAGCCGGCAGTGCTCT AAAGAGGACAAGGAGGGCGCCGACCATGGCACAGCCAAAGGCACTGCCCTCATCAGGAAGAACCCCCCAG AGTCTTCAGGGAACACAGGGAAATCCAAGGAGGAGACCCTGAAAAACCGAGTCTTCTCACTCAAGAAATC CCACAGCACTTATGACCACGTGAGAGACCAAACGGAAGAGTCCAGTAGCCTACCCACAGAAAGCCAAGAG GAGGAGACAACAGAAAATTCCACACTGGAATCCCTGTCGGGTAAAAAACTAACACAAAAACTAAAAGAAG ACAGCGAGGCTGAGTCCACGGAGTCGGTGCCGTTGGTGTGCAAGTCAGCAAGCGCTCACAACCTCAGCTC AGAGAAGAAAACTGGGCACCCACGAACATCGATGTTACAGAAGTCTCTCAGTGTCATAGCAAGCGCCAAG GAGAAGACTCTTGGATTAGCTGGGAAAACCCAAACAGCAGGTGTGGAAGAACGCACTAAATCCCAGAAAC CTTTGCCAAAAGATAAAGAGACAAACAGAAATCACTCAAATTCTGATAACACAGAGACTAAAGATCCTGC CCCCCAAAACTCAAATCCTGCGGAGGAGCCAAGAAAGCCTCAGAAATCTGGGATTATGAAACAACAAAGG GTCAACCCCACCACTGCCAATTCTGACCTGAACCCAGGCACCACCCAGATGAAGGACAACTTTGACATTG GGGAGGTGTGTCCTTGGGAGGTTTATGACCTGACCCCTGGTCCTGTGCCTTCAGAATCAAAAGTTCAAAA GCACGTATCTATTGTGGCTTCTGAAATGGAGAAAAACCCCACTTTTTCCTTAAAGGAGAAATCTCACCAC AAGCCTAAGGCAGCTGAGGTTTGTCAGCAATCCAATCAGAAGCGCATAGATAAGGCTGAAGTATGCCTTT GGGAGAGCCAAGGCCAGTCCATTTTGGAAGATGAGAAGCTTTTGATTTCCAAGACTCCAGTTCTCCCAGA GAGGGCAAAAGAGGAGAACGGAGGTCAGCCTCGTGCAGCCAATGTGTGTGCTGGGCAGAGCGAAGAACTG CCCCCCAAAGCTGTAGCATCAAAAACAGAGAATGAAAATCTCAACCAAATAGGACACCAGGAAAAAAAGA CATCTTCTTCTGAGGAGAATGTGCGTGGCTCCTATAACTCAAGTAATAACTTCCAGCAACCTTTAACATC ACGAGCAGAGGTTTGTCCTTGGGAGTTTGAGACCCCAGCTCAACCAAATGCTGGAAGAAGTGTAGCTTTA CCTGCCTCTTCTGCTCTAAGTGCAAATAAGATAGCAGGGCCTAGGAAAGAAGAGATCTGGGATAGTTTTA AAGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG214433 representing NM_020752
Red=Cloning site Green=Tags(s) MGAMAYPLLLCLLLAQLGLGAVGASRDPQGRPDSPRERTPKGKPHAQQPGRASASDSSAPWSRSTDGTIL AQKLAEEVPMDVASYLYTGDSHQLKRANCSGRYELAGLPGKWPALASAHPSLHRALDTLTHATNFLNVML QSNKSREQNLQDDLDWYQALVWSLLEGEPSISRAAITFSTDSLSAPAPQVFLQATREESRILLQDLSSSA PHLANATLETEWFHGLRRKWRPHLHRRGPNQGPRGLGHSWRRKDGLGGDKSHFKWSPPYLECENGSYKPG WLVTLSSAIYGLQPNLVPEFRGVMKVDINLQKVDIDQCSSDGWFSGTHKCHLNNSECMPIKGLGFVLGAY ECICKAGFYHPGVLPVNNFRRRGPDQHISGSTKDVSEEAYVCLPCREGCPFCADDSPCFVQEDKYLRLAI ISFQALCMLLDFVSMLVVYHFRKAKSIRASGLILLETILFGSLLLYFPVVILYFEPSTFRCILLRWARLL GFATVYGTVTLKLHRVLKVFLSRTAQRIPYMTGGRVMRMLAVILLVVFWFLIGWTSSVCQNLEKQISLIG QGKTSDHLIFNMCLIDRWDYMTAVAEFLFLLWGVYLCYAVRTVPSAFHEPRYMAVAVHNELIISAIFHTI RFVLASRLQSDWMLMLYFAHTHLTVTVTIGLLLIPKFSHSSNNPRDDIATEAYEDELDMGRSGSYLNSSI NSAWSEHSLDPEDIRDELKKLYAQLEIYKRKKMITNNPHLQKKRCSKKGLGRSIMRRITEIPETVSRQCS KEDKEGADHGTAKGTALIRKNPPESSGNTGKSKEETLKNRVFSLKKSHSTYDHVRDQTEESSSLPTESQE EETTENSTLESLSGKKLTQKLKEDSEAESTESVPLVCKSASAHNLSSEKKTGHPRTSMLQKSLSVIASAK EKTLGLAGKTQTAGVEERTKSQKPLPKDKETNRNHSNSDNTETKDPAPQNSNPAEEPRKPQKSGIMKQQR VNPTTANSDLNPGTTQMKDNFDIGEVCPWEVYDLTPGPVPSESKVQKHVSIVASEMEKNPTFSLKEKSHH KPKAAEVCQQSNQKRIDKAEVCLWESQGQSILEDEKLLISKTPVLPERAKEENGGQPRAANVCAGQSEEL PPKAVASKTENENLNQIGHQEKKTSSSEENVRGSYNSSNNFQQPLTSRAEVCPWEFETPAQPNAGRSVAL PASSALSANKIAGPRKEEIWDSFKV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020752 |
| ORF Size | 3645 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020752.3 |
| RefSeq Size | 6663 bp |
| RefSeq ORF | 3648 bp |
| Locus ID | 57512 |
| UniProt ID | Q5T848 |
| Protein Families | Druggable Genome, Transmembrane |
| Gene Summary | Orphan receptor.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214433 | GPR158 (Myc-DDK-tagged)-Human G protein-coupled receptor 158 (GPR158) |
CNY 8,464.00 |
|
| RC214433L3 | Lenti ORF clone of Human G protein-coupled receptor 158 (GPR158), Myc-DDK-tagged |
CNY 14,060.00 |
|
| RC214433L4 | Lenti ORF clone of Human G protein-coupled receptor 158 (GPR158), mGFP tagged |
CNY 14,060.00 |
|
| SC304818 | GPR158 (untagged)-Human G protein-coupled receptor 158 (GPR158) |
CNY 8,448.00 |
|
| SC318553 | GPR158 (untagged)-Human G protein-coupled receptor 158 (GPR158) |
CNY 23,470.00 |
