NORE1 (RASSF5) (NM_182663) Human Tagged ORF Clone
CAT#: RG215017
- TrueORF®
RASSF5 (tGFP-tagged) - Human Ras association (RalGDS/AF-6) domain family member 5 (RASSF5), transcript variant 1
ORF Plasmid: DDK
"NM_182663" in other vectors (6)
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CNY 5,256.00
Cited in 1 publication. |
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | Maxp1; NORE1; NORE1A; NORE1B; RAPL; RASSF3 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG215017 representing NM_182663
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCATGGCGTCCCCGGCCATCGGGCAGCGCCCGTACCCGCTACTCTTGGACCCCGAGCCGCCGCGCT ATCTACAGAGCCTGAGCGGCCCCGAGCTACCGCCGCCGCCCCCCGACCGGTCCTCGCGCCTCTGTGTCCC GGCGCCCCTCTCCACTGCGCCCGGGGCGCGCGAGGGGCGCAGCGCCCGGAGGGCTGCCCGGGGGAACCTG GAGCCCCCGCCCCGGGCCTCCCGACCCGCTCGCCCGCTCCGGCCTGGTCTGCAGCAGAGACTGCGGCGGC GGCCTGGAGCGCCCCGACCCCGCGACGTGCGGAGCATCTTCGAGCAGCCGCAGGATCCCAGAGTCCCGGC GGAGCGAGGCGAGGGGCACTGCTTCGCCGAGTTGGTGCTGCCGGGCGGCCCCGGCTGGTGTGACCTGTGC GGACGAGAGGTGCTGCGGCAGGCGCTGCGCTGCACTAACTGTAAATTCACCTGTCACCCAGAATGCCGCA GCCTGATCCAGTTGGACTGCAGTCAGCAGGAGGGTTTATCCCGGGACAGACCCTCTCCAGAAAGCACCCT CACCGTGACCTTCAGCCAGAATGTCTGTAAACCTGTGGAGGAGACACAGCGCCCGCCCACACTGCAGGAG ATCAAGCAGAAGATCGACAGCTACAACACGCGAGAGAAGAACTGCCTGGGCATGAAACTGAGTGAAGACG GCACCTACACGGGTTTCATCAAAGTGCATCTGAAACTCCGGCGGCCTGTGACGGTGCCTGCTGGGATCCG GCCCCAGTCCATCTATGATGCCATCAAGGAGGTGAACCTGGCGGCTACCACGGACAAGCGGACATCCTTC TACCTGCCCCTAGATGCCATCAAGCAGCTGCACATCAGCAGCACCACCACCGTCAGTGAGGTCATCCAGG GGCTGCTCAAGAAGTTCATGGTTGTGGACAATCCCCAGAAGTTTGCACTTTTTAAGCGGATACACAAGGA CGGACAAGTGCTCTTCCAGAAACTCTCCATTGCTGACCGCCCCCTCTACCTGCGCCTGCTTGCTGGGCCT GACACGGAGGTCCTCAGCTTTGTGCTAAAGGAGAATGAAACTGGAGAGGTAGAGTGGGATGCCTTCTCCA TCCCTGAACTTCAGAACTTCCTAACAATCCTGGAAAAAGAGGAGCAGGACAAAATCCAACAAGTGCAAAA GAAGTATGACAAGTTTAGGCAGAAACTGGAGGAGGCCTTAAGAGAATCCCAGGGCAAACCTGGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG215017 representing NM_182663
Red=Cloning site Green=Tags(s) MAMASPAIGQRPYPLLLDPEPPRYLQSLSGPELPPPPPDRSSRLCVPAPLSTAPGAREGRSARRAARGNL EPPPRASRPARPLRPGLQQRLRRRPGAPRPRDVRSIFEQPQDPRVPAERGEGHCFAELVLPGGPGWCDLC GREVLRQALRCTNCKFTCHPECRSLIQLDCSQQEGLSRDRPSPESTLTVTFSQNVCKPVEETQRPPTLQE IKQKIDSYNTREKNCLGMKLSEDGTYTGFIKVHLKLRRPVTVPAGIRPQSIYDAIKEVNLAATTDKRTSF YLPLDAIKQLHISSTTTVSEVIQGLLKKFMVVDNPQKFALFKRIHKDGQVLFQKLSIADRPLYLRLLAGP DTEVLSFVLKENETGEVEWDAFSIPELQNFLTILEKEEQDKIQQVQKKYDKFRQKLEEALRESQGKPG TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_182663 |
ORF Size | 1254 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_182663.1, NP_872604.1 |
RefSeq Size | 2048 bp |
RefSeq ORF | 1257 bp |
Locus ID | 83593 |
UniProt ID | Q8WWW0 |
Protein Families | Druggable Genome |
Protein Pathways | Leukocyte transendothelial migration, Non-small cell lung cancer, Pathways in cancer |
Gene Summary | This gene is a member of the Ras association domain family. It functions as a tumor suppressor, and is inactivated in a variety of cancers. The encoded protein localizes to centrosomes and microtubules, and associates with the GTP-activated forms of Ras, Rap1, and several other Ras-like small GTPases. The protein regulates lymphocyte adhesion and suppresses cell growth in response to activated Rap1 or Ras. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
---|
NORE1A is a Ras senescence effector that controls the apoptotic/senescent balance of p53 via HIPK2
,Donninger, H;Calvisi, DF;Barnoud, T;Clark, J;Schmidt, ML;Vos, MD;Clark, GJ;,
J. Cell Biol.
,PubMed ID 25778922
[RASSF5]
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Documents
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC215017 | RASSF5 (Myc-DDK-tagged)-Human Ras association (RalGDS/AF-6) domain family member 5 (RASSF5), transcript variant 1 |
CNY 3,656.00 |
|
RC215017L1 | Lenti ORF clone of Human Ras association (RalGDS/AF-6) domain family member 5 (RASSF5), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
RC215017L2 | Lenti ORF clone of Human Ras association (RalGDS/AF-6) domain family member 5 (RASSF5), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RC215017L3 | Lenti ORF clone of Human Ras association (RalGDS/AF-6) domain family member 5 (RASSF5), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC215017L4 | Lenti ORF clone of Human Ras association (RalGDS/AF-6) domain family member 5 (RASSF5), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
SC307476 | RASSF5 (untagged)-Human Ras association (RalGDS/AF-6) domain family member 5 (RASSF5), transcript variant 1 |
CNY 6,750.00 |