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ALG10B (NM_001013620) Human Tagged ORF Clone
CAT＃: RG215330
- TrueORF®
ALG10B (tGFP-tagged) - Human asparagine-linked glycosylation 10, alpha-1,2-glucosyltransferase homolog B (yeast) (ALG10B)
ORF Plasmid: DDK
"NM_001013620" in other vectors (4)
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CNY 4,370.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ALG10; KCR1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG215330 representing NM_001013620
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCAGCTAGAGGGTTACTGTTTCTCGGCCGCCTTGAGCTGTACCTTTTTAGTGTCCTGCCTCCTCT TCTCCGCCTTCAGCCGGGCGCTGCGAGAGCCCTACATGGACGAGATCTTCCACCTGCCTCAGGCGCAGCG CTACTGTGAGGGCCATTTCTCCCTTTCCCAGTGGGATCCCATGATTACTACATTACCTGGCTTGTACCTG GTGTCAGTTGGAGTGGTCAAACCTGCCATTTGGATCTTTGGATGGTCTGAACATGTTGTCTGCTCCATTG GGATGCTCAGATTTGTTAATCTTCTCTTCAGTGTTGGCAACTTCTATTTACTATATTTGCTTTTCCACAA GGTACAACCCAGAAACAAGGCTGCCTCAAGTATCCAGAGAGTCTTGTCAACATTAACACTAGCAGTATTT CCAACACTTTATTTTTTTAACTTCCTTTATTATACAGAAGCAGGATCTATGTTTTTTACTCTTTTTGCAT ATTTGATGTGTCTTTATGGAAATCATAAAACTTCAGCCTTCCTTGGATTTTGTGGCTTCATGTTTCGGCA AACAAATATCATCTGGGCTGTCTTCTGTGCAGGGAATGTCATTGCACAAAAGTTAACTGAGGCTTGGAAA ACTGAGCTACAAAAGAAGGAAGACAGACTTCCACCTATTAAAGGACCATTTGCAGAATTCAGAAAAATTC TTCAGTTTCTTTTGGCTTATTCCATGTCCTTTAAAAACTTGAGTATGCTTTTCTGTTTGACTTGGCCCTA CATCCTTCTGGGATTTCTGTTTTGTGCTTTTGTAGTAGTTAATGGTGGAATTGTTATTGGCGATCGGAGT AGTCATGAAGCCTGTCTTCATTTTCCTCAACTATTCTACTTTTTTTCATTTACTCTCTTTTTTTCTTTTC CTCATCTCCTGTCTCCTAGCAAAATTAAGACTTTTCTTTCCTTAGTTTGGAAACATGGAATTCTGTTTTT GGTGGTTACCTTAGTCTCTGTGTTTTTAGTTTGGAAATTCACTTATGCTCATAAATACTTGCTAGCAGAC AATAGACATTATACTTTCTATGTGTGGAAAAGAGTTTTTCAAAGATATGCAATTCTGAAATATTTGTTAG TTCCAGCCTATATATTTGCTGGTTGGAGTATAGCTGACTCATTGAAATCAAAGCCAATTTTTTGGAATTT AATGTTTTTCATATGCTTGTTCATTGTTATAGTTCCTCAGAAACTGCTGGAATTTCGTTACTTCATTTTA CCTTATGTCATTTATAGGCTTAACATAACTCTGCCTCCCACATCCAGACTTGTTTGTGAACTGAGTTGCT ATGCAATTGTTAATTTCATAACTTTTTACATCTTTCTGAACAAGACTTTTCAGTGGCCAAATAGTCAGGA CATTCAAAGGTTTATGTGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG215330 representing NM_001013620
Red=Cloning site Green=Tags(s) MAQLEGYCFSAALSCTFLVSCLLFSAFSRALREPYMDEIFHLPQAQRYCEGHFSLSQWDPMITTLPGLYL VSVGVVKPAIWIFGWSEHVVCSIGMLRFVNLLFSVGNFYLLYLLFHKVQPRNKAASSIQRVLSTLTLAVF PTLYFFNFLYYTEAGSMFFTLFAYLMCLYGNHKTSAFLGFCGFMFRQTNIIWAVFCAGNVIAQKLTEAWK TELQKKEDRLPPIKGPFAEFRKILQFLLAYSMSFKNLSMLFCLTWPYILLGFLFCAFVVVNGGIVIGDRS SHEACLHFPQLFYFFSFTLFFSFPHLLSPSKIKTFLSLVWKHGILFLVVTLVSVFLVWKFTYAHKYLLAD NRHYTFYVWKRVFQRYAILKYLLVPAYIFAGWSIADSLKSKPIFWNLMFFICLFIVIVPQKLLEFRYFIL PYVIYRLNITLPPTSRLVCELSCYAIVNFITFYIFLNKTFQWPNSQDIQRFMW TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001013620 |
| ORF Size | 1419 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001013620.3, NP_001013642.1 |
| RefSeq Size | 1623 bp |
| RefSeq ORF | 1422 bp |
| Locus ID | 144245 |
| UniProt ID | Q5I7T1 |
| Protein Families | Transmembrane |
| Protein Pathways | Metabolic pathways, N-Glycan biosynthesis |
| Gene Summary | Putative alpha-1,2-glucosyltransferase, which adds the third glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc(2)Man(9)GlcNAc(2)-PP-Dol (By similarity). When coupled to KCNH2 may reduce KCNH2 sensitivity to classic proarrhythmic drug blockade, possibly by mediating glycosylation of KCNH2 (PubMed:14525949). Has a role in maintenance of cochlear outer hair cell function (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215330 | ALG10B (Myc-DDK-tagged)-Human asparagine-linked glycosylation 10, alpha-1,2-glucosyltransferase homolog B (yeast) (ALG10B) |
CNY 3,656.00 |
|
| RC215330L3 | Lenti ORF clone of Human asparagine-linked glycosylation 10, alpha-1,2-glucosyltransferase homolog B (yeast) (ALG10B), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC215330L4 | Lenti ORF clone of Human asparagine-linked glycosylation 10, alpha-1,2-glucosyltransferase homolog B (yeast) (ALG10B), mGFP tagged |
CNY 5,890.00 |
|
| SC301795 | ALG10B (untagged)-Human asparagine-linked glycosylation 10, alpha-1,2-glucosyltransferase homolog B (yeast) (ALG10B) |
CNY 7,220.00 |
