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WDR27 (NM_182552) Human Tagged ORF Clone
CAT＃: RG215385
- TrueORF®
WDR27 (tGFP-tagged) - Human WD repeat domain 27 (WDR27), transcript variant 1
ORF Plasmid: DDK
"NM_182552" in other vectors (4)
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CNY 7,890.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG215385 representing NM_182552
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAATCCCCAAGACATTTTCTCAAGTAATGGTGGCTGTCTAAGTGATATAGTTATAGAAAAATACC TGGTTGAATCCAAGGAGTCTGTGTCTCATGTTCAGCTTGCTTGCAGCATGCAGGACTGTGCTTTCCCTTT GGATGGAACTGAACTTTGTATATGGAACACTAAGGATCCTTCTCATCAGCTTCTAATCCTACGAGGACAC CATCAGCCAATTACTGCTATGGCTTTTGGAAATAAAGTGAACCCACTTCTAATCTGCTCAGCATCACTGG ATTATGTTATAATGTGGAACCTGGATGAATGTAGAGAGAAAGTACTTCAAGGGCTGGTCCCTCGAGGGAC TGTCATGGGCTCGCTTTTGGGAAAGGTGCTGTGTTTACAGTTGAGCCTGGATGATCATGTTGTTGCCGTG TGTGCTGGAAACAAAATATTCATGCTGGATATTGAGCAGCGATTTTCTGTGACATACATAGAACGTCCTG ATGTTAATAACCGCCACAAAGTCCCACCACCGACCTTCCTGCACACATTCTCTCAGACTCAGGCTGTTCG GGCCGAGCTGCAGGGCCACCTGGGCCCGGTGACTGCGGTGGAGTTCTGTCCCTGGCGAGCAGGCACCCTC ATCTCGGCGTCTGAGGACAGAGGCTTTAAGGTCTGGGACCATTGTACAGGATCATTAATATACAGTTCAT CTGTGTTATCAGCATATCCTCTTCTCAGTTTATTCATTGATGCAGAAAGCAGGCAGCTGGTCACCGGGTG TGCTGACGGCCAGCTTTGGATCTTCAGTTTGATGGATGGACACCATTATCGTCGTGTGGCACGGGTTGAC CTAAGGAAGAAGACAGAGACTTTCTCCACAAGAAGGGTTAAGTCTGGGCTGTGCAGCCAGCCAGAAGAAA GCCAGCTTCCCTCTACAAGTGCTCTGGGAAAAGGAGAGCAGGTTGAAGTAACATTTCCTGTACTGAGACT TGCACCCTGTGATCTCTCACTCATCCCAAATTCTGCATGTGGATGTCTTTCTTCTGAGAACACCCGATGT GTGTGGATCGGAAGCTCAGTGGGCTTATTCGTATTTAACCTGGCAAACCTGGAAGTGGAAGCTGCTTTGT ATTACAAGGATTTCCAGAGCCTCAGCATCCTGCTGGCCGGATCGTGTGCCCTGAGGAACCGCACTGCGGA TCAAAAGGTGCTGTGCTTGCTGGCCTCCCTCTTTGGCGGGAAGATTGCCGTGTTGGAGATCAACCCGGCC GCGCTAGTCAGGGCTCAGCAGTGCCCCAGCATGGGGCAGAGCCTCTCGGTGCCAGCCAGCTCCTGTGTCC TACCGACCTCTCCACTGTATCTGGGAATTGCCAAGGAGAAGAGTACCAAGGCTGCTAGTGAACAGCGACG TGCTGCACGGAACGTCATGAAGGACCAACGCCTGGTTTTCCATAGTAAAGTTAGGTCATCTGGTTATGCG TCAGCACCACATGTAACTATGTTTTCACCAAAGACCAACATCAAGAGTGAGGGGAAAGGATCTTCAAGGA GCAGGAGCAGCTGCGCACGGGAGGCATACCCCGTGGAGTGCGCTGTGCCCACCAAGCCCGGCCCCCAGGT CGCTGCCGCCCCCACCTGCACACGTGTATGCTGCATCCAGTACTCAGGAGATGGGCAGTGGCTGGCCTGT GGGTTGGCCAACCATCTGTTACTCGTTTTTGATGCCAGCCTGACTGGGACACCTGCTGTGTTTTCAGGTC ACGACGGGGCAGTGAATGCCGTGTGCTGGAGCCAGGACCGGAGGTGGCTGCTCTCTGCGGCCCGGGACGG GACCCTGCGAATGTGGTCGGCTCGTGGGGCAGAGCTCGCACTGCTTCTGGGCAAAGACATGTTTTCTAAA CCTATACAGTCTGCACAGTTCTATTATATAGATGCCTTTATATTGTTATCTTCTGGCCCTGAATTTCAGC TACTGAGGTATCACATTGACACTTGCAAAGATGAGATTAAGAGATATAAACAGAAGAGCAAGTCCAAGCT GATTTGCAGGCTCTCCACGACGGGTGCAGTAGACATGACCAGTTTATCGGCAGTCAACGACTTTTATTCC CACATCGTACTCGCAGCTGGCCGGAACAGGACCGTGGAAGTGTTTGACCTCAACGCCGGCTGCAGTGCAG CGGTGATAGCGGAAGCCCACTCACGGCCTGTCCATCAAATCTGCCAAAATAAAGGTTCATCATTTACAAC CCAACAGCCTCAGGCTTATAACCTTTTCCTGACCACGGCCATTGGCGATGGGATGAGACTGTGGGACCTG AGAACCCTGAGGTGTGAGCGCCACTTTGAAGGGCATCCAACCCGCGGCTATCCATGTGGAATCGCTTTCA GTCCTTGTGGACGATTCGCGGCTTGTGGGGCCGAGGACAGACACGCTTACGTGTATGAAATGGGCTCAAG CACGTTTTCTCACCGGCTGGCTGGGCACACAGACACTGTCACTGGAGTGGCCTTCAACCCATCAGCGCCC CAGGTACAGTCTTGCTCTGTCGCCCACGCTGGAGTGCAGTGGCACGATCTCGGTTCACTGCAACCTCCGC CTTCCAGGTTCAAGCAATTCTTCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGCTTGCCACAGCTACC TTGGATGGTAAACTCCAGCTCTTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG215385 representing NM_182552
Red=Cloning site Green=Tags(s) MENPQDIFSSNGGCLSDIVIEKYLVESKESVSHVQLACSMQDCAFPLDGTELCIWNTKDPSHQLLILRGH HQPITAMAFGNKVNPLLICSASLDYVIMWNLDECREKVLQGLVPRGTVMGSLLGKVLCLQLSLDDHVVAV CAGNKIFMLDIEQRFSVTYIERPDVNNRHKVPPPTFLHTFSQTQAVRAELQGHLGPVTAVEFCPWRAGTL ISASEDRGFKVWDHCTGSLIYSSSVLSAYPLLSLFIDAESRQLVTGCADGQLWIFSLMDGHHYRRVARVD LRKKTETFSTRRVKSGLCSQPEESQLPSTSALGKGEQVEVTFPVLRLAPCDLSLIPNSACGCLSSENTRC VWIGSSVGLFVFNLANLEVEAALYYKDFQSLSILLAGSCALRNRTADQKVLCLLASLFGGKIAVLEINPA ALVRAQQCPSMGQSLSVPASSCVLPTSPLYLGIAKEKSTKAASEQRRAARNVMKDQRLVFHSKVRSSGYA SAPHVTMFSPKTNIKSEGKGSSRSRSSCAREAYPVECAVPTKPGPQVAAAPTCTRVCCIQYSGDGQWLAC GLANHLLLVFDASLTGTPAVFSGHDGAVNAVCWSQDRRWLLSAARDGTLRMWSARGAELALLLGKDMFSK PIQSAQFYYIDAFILLSSGPEFQLLRYHIDTCKDEIKRYKQKSKSKLICRLSTTGAVDMTSLSAVNDFYS HIVLAAGRNRTVEVFDLNAGCSAAVIAEAHSRPVHQICQNKGSSFTTQQPQAYNLFLTTAIGDGMRLWDL RTLRCERHFEGHPTRGYPCGIAFSPCGRFAACGAEDRHAYVYEMGSSTFSHRLAGHTDTVTGVAFNPSAP QVQSCSVAHAGVQWHDLGSLQPPPSRFKQFFCLSLPSSWDYSLPQLPWMVNSSSF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_182552 |
| ORF Size | 2685 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_182552.2, NP_872358.2 |
| RefSeq Size | 3193 bp |
| RefSeq ORF | 2688 bp |
| Locus ID | 253769 |
| UniProt ID | A2RRH5 |
| Gene Summary | This gene encodes a protein with multiple WD repeats. Proteins with these repeats may form scaffolds for protein-protein interaction and play key roles in cell signalling. Alternative splicing results in multiple transcript variants, but the full-length structure of some of these variants cannot be determined. [provided by RefSeq, Nov 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215385 | WDR27 (Myc-DDK-tagged)-Human WD repeat domain 27 (WDR27), transcript variant 1 |
CNY 5,904.00 |
|
| RC215385L3 | Lenti ORF clone of Human WD repeat domain 27 (WDR27), transcript variant 1, Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC215385L4 | Lenti ORF clone of Human WD repeat domain 27 (WDR27), transcript variant 1, mGFP tagged |
CNY 9,030.00 |
|
| SC314880 | WDR27 (untagged)-Human WD repeat domain 27 (WDR27), transcript variant 1 |
CNY 16,530.00 |
