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Semenogelin I (SEMG1) (NM_003007) Human Tagged ORF Clone
CAT＃: RG216046
- TrueORF®
SEMG1 (tGFP-tagged) - Human semenogelin I (SEMG1)
ORF Plasmid: DDK
"NM_003007" in other vectors (4)
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CNY 4,470.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CT103; dJ172H20.2; SEMG; SGI |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG216046 representing NM_003007
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCCCAACATCATCTTTGTACTTTCCCTGCTCCTCATCTTGGAGAAGCAAGCAGCTGTGATGGGAC AAAAAGGTGGATCAAAAGGCCGATTACCAAGTGAATTTTCCCAATTTCCACACGGACAAAAGGGCCAGCA CTATTCTGGACAAAAAGGCAAGCAACAAACTGAATCCAAAGGCAGTTTTTCTATTCAATACACATATCAT GTAGATGCCAATGATCATGACCAGTCCCGAAAAAGTCAGCAATATGATTTGAATGCCCTACATAAGACGA CAAAATCACAACGACATCTAGGTGGAAGTCAACAACTGCTCCATAATAAACAAGAAGGCAGAGACCATGA TAAATCAAAAGGTCATTTTCACAGGGTAGTTATACACCATAAAGGAGGCAAAGCTCATCGTGGGACACAA AATCCTTCTCAAGATCAGGGGAATAGCCCATCTGGAAAGGGAATATCCAGTCAATATTCAAACACAGAAG AAAGGCTGTGGGTTCATGGACTAAGTAAAGAACAAACTTCCGTCTCTGGTGCACAAAAAGGTAGAAAACA AGGCGGATCCCAAAGCAGTTATGTTCTCCAAACTGAAGAGCTAGTAGCTAACAAACAACAACGTGAGACT AAAAATTCTCATCAAAATAAAGGGCATTACCAAAATGTGGTTGAAGTGAGAGAGGAACATTCAAGTAAAG TACAAACCTCACTCTGTCCTGCGCACCAAGACAAACTCCAACATGGATCCAAAGACATTTTTTCTACCCA AGATGAGCTCCTAGTATATAACAAGAATCAACACCAGACAAAAAATCTCAATCAAGATCAACAGCATGGC CGAAAGGCAAATAAAATATCATACCAATCTTCAAGTACAGAAGAAAGACGACTCCACTATGGAGAAAATG GTGTGCAGAAAGATGTATCCCAAAGCAGTATTTATAGCCAAACTGAAGAGAAAGCACAGGGCAAGTCTCA AAAACAGATAACAATTCCCAGTCAAGAGCAAGAGCATAGCCAAAAGGCAAATAAAATATCATACCAATCT TCAAGTACGGAAGAAAGACGACTCCACTATGGAGAAAATGGTGTGCAGAAAGATGTATCCCAACGCAGTA TTTATAGCCAAACTGAAAAGCTAGTAGCAGGCAAGTCTCAAATCCAGGCACCAAATCCTAAGCAAGAGCC ATGGCATGGTGAAAATGCAAAAGGAGAGTCTGGCCAATCTACAAATAGAGAACAAGACCTACTCAGTCAT GAACAAAAAGGCAGACACCAACATGGATCTCATGGGGGATTGGATATTGTAATTATAGAGCAGGAAGATG ACAGTGATCGTCATTTGGCACAACATCTTAACAACGACCGAAACCCATTATTTACA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG216046 representing NM_003007
Red=Cloning site Green=Tags(s) MKPNIIFVLSLLLILEKQAAVMGQKGGSKGRLPSEFSQFPHGQKGQHYSGQKGKQQTESKGSFSIQYTYH VDANDHDQSRKSQQYDLNALHKTTKSQRHLGGSQQLLHNKQEGRDHDKSKGHFHRVVIHHKGGKAHRGTQ NPSQDQGNSPSGKGISSQYSNTEERLWVHGLSKEQTSVSGAQKGRKQGGSQSSYVLQTEELVANKQQRET KNSHQNKGHYQNVVEVREEHSSKVQTSLCPAHQDKLQHGSKDIFSTQDELLVYNKNQHQTKNLNQDQQHG RKANKISYQSSSTEERRLHYGENGVQKDVSQSSIYSQTEEKAQGKSQKQITIPSQEQEHSQKANKISYQS SSTEERRLHYGENGVQKDVSQRSIYSQTEKLVAGKSQIQAPNPKQEPWHGENAKGESGQSTNREQDLLSH EQKGRHQHGSHGGLDIVIIEQEDDSDRHLAQHLNNDRNPLFT TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003007 |
| ORF Size | 1386 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003007.5 |
| RefSeq Size | 1649 bp |
| RefSeq ORF | 1389 bp |
| Locus ID | 6406 |
| UniProt ID | P04279 |
| Protein Families | Secreted Protein |
| Gene Summary | The protein encoded by this gene is the predominant protein in semen. The encoded secreted protein is involved in the formation of a gel matrix that encases ejaculated spermatozoa. This preproprotein is proteolytically processed by the prostate-specific antigen (PSA) protease to generate multiple peptide products that exhibit distinct functions. One of these peptides, SgI-29, is an antimicrobial peptide with antibacterial activity. This proteolysis process also breaks down the gel matrix and allows the spermatozoa to move more freely. This gene and another similar semenogelin gene are present in a gene cluster on chromosome 20. [provided by RefSeq, Feb 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216046 | SEMG1 (Myc-DDK-tagged)-Human semenogelin I (SEMG1) |
CNY 3,656.00 |
|
| RC216046L3 | Lenti-ORF clone of SEMG1 (Myc-DDK-tagged)-Human semenogelin I (SEMG1) |
CNY 5,990.00 |
|
| RC216046L4 | Lenti-ORF clone of SEMG1 (mGFP-tagged)-Human semenogelin I (SEMG1) |
CNY 5,990.00 |
|
| SC303261 | SEMG1 (untagged)-Human semenogelin I (SEMG1) |
CNY 3,656.00 |
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