PCGF3 (NM_006315) Human Tagged ORF Clone

CAT#: RG216111

  • TrueORF®

PCGF3 (tGFP-tagged) - Human polycomb group ring finger 3 (PCGF3)

ORF Plasmid: DDK tGFP



  "NM_006315" in other vectors (4)

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CNY 4,370.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Rabbit Polyclonal Anti-PCGF3 Antibody
    • 50 ug

CNY 5,250.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms DONG1; RNF3; RNF3A
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG216111 representing NM_006315
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGACCAGGAAGATCAAGCTGTGGGACATCAACGCCCACATCACCTGCCGCCTGTGCAGCGGGTACC
TCATCGACGCCACCACGGTGACCGAGTGTCTGCACACCTTCTGCAGGAGCTGCCTGGTGAAGTACCTGGA
GGAGAACAACACCTGCCCCACCTGCAGGATTGTGATCCACCAGAGCCACCCCCTGCAGTACATCGGTCAT
GACAGAACCATGCAAGATATTGTTTACAAATTGGTACCAGGCCTCCAAGAAGCGGAAATGAGAAAGCAGA
GGGAGTTCTATCACAAATTGGGCATGGAGGTGCCGGGAGACATCAAGGGGGAGACCTGCTCTGCAAAACA
GCACTTAGATTCCCATCGGAATGGTGAAACCAAAGCAGACGACAGTTCAAACAAAGAGGCCGCGGAGGAG
AAGCCGGAGGAGGACAACGACTACCACCGCAGCGACGAGCAGGTGAGCATCTGCCTGGAGTGTAACAGCA
GCAAACTGCGCGGGCTGAAGCGGAAGTGGATCCGCTGCTCAGCCCAGGCGACCGTCTTGCATCTGAAGAA
GTTCATCGCCAAAAAACTCAACCTTTCATCCTTTAACGAGCTGGACATTTTATGCAACGAGGAGATCCTG
GGCAAGGACCACACACTCAAGTTCGTGGTTGTCACTAGGTGGAGATTCAAGAAGGCGCCGCTCCTGCTGC
ACTACAGACCCAAGATGGACTTGCTG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG216111 representing NM_006315
Red=Cloning site Green=Tags(s)

MLTRKIKLWDINAHITCRLCSGYLIDATTVTECLHTFCRSCLVKYLEENNTCPTCRIVIHQSHPLQYIGH
DRTMQDIVYKLVPGLQEAEMRKQREFYHKLGMEVPGDIKGETCSAKQHLDSHRNGETKADDSSNKEAAEE
KPEEDNDYHRSDEQVSICLECNSSKLRGLKRKWIRCSAQATVLHLKKFIAKKLNLSSFNELDILCNEEIL
GKDHTLKFVVVTRWRFKKAPLLLHYRPKMDLL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_006315
ORF Size 726 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006315.6
RefSeq Size 5666 bp
RefSeq ORF 729 bp
Locus ID 10336
UniProt ID Q3KNV8
Domains RING
Gene Summary The protein encoded by this gene contains a C3HC4 type RING finger, which is a motif known to be involved in protein-protein interactions. The specific function of this protein has not yet been determined. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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