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Nephrocystin 4 (NPHP4) (NM_015102) Human Tagged ORF Clone
CAT＃: RG216464
- TrueORF®
NPHP4 (tGFP-tagged) - Human nephronophthisis 4 (NPHP4)
ORF Plasmid: DDK
"NM_015102" in other vectors (6)
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CNY 11,880.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | POC10; SLSN4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG216464 representing NM_015102
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACGACTGGCACAGGATCTTCACCCAAAACGTGCTTGTCCCTCCCCACCCACAGAGAGCGCGCCAGC CTTGGAAGGAATCCACGGCATTCCAGTGTGTCCTCAAGTGGCTGGACGGACCGGTAATTAGGCAGGGCGT GCTGGAGGTACTGTCAGAGGTTGAATGCCATCTGCGAGTGTCTTTCTTTGATGTCACCTACCGGCACTTC TTTGGGAGGACGTGGAAAACCACAGTGAAGCCGACGAAGAGACCGCCGTCCAGGATCGTCTTTAATGAGC CCTTGTATTTTCACACATCCCTAAACCACCCTCATATCGTGGCTGTGGTGGAAGTGGTCGCTGAGGGCAA GAAACGGGATGGGAGCCTCCAGACATTGTCCTGTGGGTTTGGAATTCTTCGGATCTTCAGCAACCAGCCG GACTCTCCTATCTCTGCTTCCCAGGACAAAAGGTTGCGGCTGTACCATGGCACCCCCAGAGCCCTCCTGC ACCCGCTTCTCCAGGACCCCGCAGAGCAAAACAGACACATGACCCTCATTGAGAACTGCAGCCTGCAGTA CACGCTGAAGCCACACCCGGCCCTGGAGCCTGCGTTCCACCTTCTTCCTGAGAACCTTCTGGTGTCTGGT CTGCAGCAGATACCTGGCCTGCTTCCAGCTCATGGAGAATCCGGCGACGCTCTCCGAAAGCCTCGCCTCC AGAAGCCCATCACGGGGCACTTGGATGACTTATTCTTCACCCTGTACCCCTCCCTGGAGAAGTTTGAGGA AGAGCTGCTGGAGCTCCACGTCCAGGACCACTTCCAGGAGGGATGTGGCCCACTGGACGGTGGTGCCCTG GAGATCCTGGAGCGGCGCCTGCGTGTGGGCGTGCACAATGGTCTGGGCTTCGTGCAGAGGCCGCAGGTCG TTGTACTGGTGCCTGAGATGGATGTGGCCTTGACGCGCTCAGCTAGCTTCAGCAGGAAAGTGGTCTCCTC TTCCAAGACCAGCTCCGGGAGCCAAGCTCTGGTTTTGAGAAGCCGCCTCCGCCTCCCAGAGATGGTCGGC CACCCTGCATTTGCGGTCATCTTCCAGCTGGAGTACGTGTTCAGCAGCCCTGCAGGAGTGGACGGCAATG CAGCTTCGGTCACCTCTCTGTCCAACCTGGCATGCATGCACATGGTCCGCTGGGCTGTTTGGAACCCCTT GCTGGAAGCTGATTCTGGAAGGGTGACCCTGCCTCTGCAGGGTGGGATCCAGCCCAACCCCTCGCACTGT CTGGTCTACAAGGTACCCTCAGCCAGCATGAGCTCTGAAGAGGTGAAGCAGGTGGAGTCGGGTACACTCC GGTTCCAGTTCTCGCTGGGCTCAGAAGAACACCTGGATGCACCCACGGAGCCTGTCAGTGGCCCCAAAGT GGAGCGGCGGCCTTCCAGGAAACCACCCACGTCCCCTTCGAGCCCGCCAGCGCCAGTACCTCGAGTTCTC GCTGCCCCGCAGAACTCACCTGTGGGACCAGGGTTGTCAATTTCCCAGCTGGCGGCCTCCCCGCGGTCCC CGACTCAGCACTGCTTGGCCAGGCCTACTTCACAGCTACCCCATGGCTCTCAGGCCTCCCCGGCCCAGGC ACAGGAGTTCCCGTTGGAGGCCGGTATCTCCCACCTGGAAGCCGACCTGAGCCAGACCTCCCTGGTCCTG GAAACATCCATTGCCGAACAGTTACAGGAGCTGCCGTTCACGCCTTTGCATGCCCCTATTGTTGTGGGAA CCCAGACCAGGAGCTCTGCAGGGCAGCCCTCGAGAGCCTCCATGGTGCTCCTGCAGTCCTCCGGCTTTCC CGAGATTCTGGATGCCAATAAACAGCCAGCCGAGGCTGTCAGCGCTACAGAACCTGTGACGTTTAACCCT CAGAAGGAAGAATCAGATTGTCTACAAAGCAACGAGATGGTGCTACAGTTTCTTGCCTTTAGCAGAGTGG CCCAGGACTGCCGAGGAACATCATGGCCAAAGACTGTGTATTTCACCTTCCAGTTCTACCGCTTCCCACC CGCAACGACGCCACGACTGCAGCTGGTCCAGCTGGATGAGGCCGGCCAGCCCAGCTCTGGCGCCCTGACC CACATCCTCGTGCCTGTGAGCAGAGATGGCACCTTTGATGCTGGGTCTCCTGGCTTCCAGCTGAGGTACA TGGTGGGCCCTGGGTTCCTGAAGCCAGGTGAGCGGCGCTGCTTTGCCCGCTACCTGGCCGTGCAGACCCT GCAGATTGACGTCTGGGACGGAGACTCCCTGCTGCTCATCGGATCTGCTGCCGTCCAGATGAAGCATCTC CTCCGCCAAGGCCGGCCGGCTGTGCAGGCCTCCCACGAGCTTGAGGTCGTGGCAACTGAATACGAGCAGG ACAACATGGTGGTGAGTGGAGACATGCTGGGGTTTGGCCGCGTCAAGCCCATCGGCGTCCACTCGGTGGT GAAGGGCCGGCTGCACCTGACTTTGGCCAACGTGGGTCACCCGTGTGAACAGAAAGTGAGAGGTTGTAGC ACATTGCCACCGTCCAGATCTCGGGTCATCTCAAACGATGGAGCCAGCCGCTTCTCTGGAGGCAGCCTCC TCACGACTGGAAGCTCAAGGCGAAAACACGTGGTGCAAGCACAGAAGCTGGCGGACGTGGACAGTGAGCT GGCTGCCATGCTACTGACCCATGCCCGGCAGGGCAAGGGGCCCCAGGACGTCAGCCGCGAGTCGGATGCC ACCCGCAGGCGTAAGCTGGAGCGGATGAGGTCTGTGCGCCTGCAGGAGGCCGGGGGAGACTTGGGCCGGC GCGGGACGAGCGTGTTGGCGCAGCAGAGCGTCCGCACACAGCACTTGCGGGACCTACAGGTCATCGCCGC CTACCGGGAACGCACGAAGGCCGAGAGCATCGCCAGCCTGCTGAGCCTGGCCATCACCACGGAGCACACG CTCCACGCCACGCTGGGGGTCGCCGAGTTCTTTGAGTTTGTGCTTAAGAACCCCCACAACACACAGCACA CGGTGACTGTGGAGATCGACAACCCCGAGCTCAGCGTCATCGTGGACAGTCAGGAGTGGAGGGACTTCAA GGGTGCTGCTGGCCTGCACACACCGGTGGAGGAGGACATGTTCCACCTGCGTGGCAGCCTGGCCCCCCAG CTCTACCTGCGCCCCCACGAGACCGCCCACGTCCCCTTCAAGTTCCAGAGCTTCTCTGCAGGGCAGCTGG CCATGGTGCAGGCCTCTCCTGGGTTGAGCAACGAGAAGGGCATGGACGCCGTGTCACCTTGGAAGTCCAG CGCAGTGCCCACTAAACACGCCAAGGTCTTGTTCCGAGCGAGTGGTGGCAAGCCCATCGCCGTGCTCTGC CTGACTGTGGAGCTGCAGCCCCACGTGGTGGACCAGGTCTTCCGCTTCTATCACCCGGAGCTCTCCTTCC TGAAGAAGGCCATCCGCCTGCCGCCCTGGCACACATTTCCAGGTGCTCCGGTGGGAATGCTTGGTGAGGA CCCCCCAGTCCATGTTCGCTGCAGCGACCCGAACGTCATCTGTGAGACCCAGAATGTGGGCCCCGGGGAA CCACGGGACATATTTCTGAAGGTGGCCAGTGGTCCAAGCCCGGAGATCAAAGACTTCTTTGTCATCATTT ACTCGGATCGCTGGCTGGCGACACCCACACAGACGTGGCAGGTCTACCTCCACTCCCTGCAGCGCGTGGA TGTCTCCTGCGTCGCAGGCCAGCTGACCCGCCTGTCCCTTGTCCTTCGGGGGACACAGACAGTGAGGAAA GTGAGAGCTTTCACCTCTCATCCCCAGGAGCTGAAGACAGACCCCAAAGGTGTCTTCGTGCTGCCGCCTC GTGGGGTGCAGGACCTGCATGTTGGCGTGAGGCCCCTTAGGGCCGGCAGCCGCTTTGTCCATCTCAACCT GGTGGACGTGGATTGCCACCAGCTGGTGGCCTCCTGGCTCGTGTGCCTCTGCTGCCGCCAGCCGCTCATC TCCAAGGCCTTTGAGATCATGTTGGCTGCGGGCGAAGGGAAGGGTGTCAACAAGAGGATCACCTACACCA ACCCCTACCCCTCCCGGAGGACATTCCACCTGCACAGCGACCACCCGGAGCTGCTGCGGTTCAGAGAGGA CTCCTTCCAGGTCGGGGGTGGAGAGACCTACACCATCGGCTTGCAGTTTGCGCCTAGTCAGAGAGTGGGT GAGGAGGAGATCCTGATCTACATCAATGACCATGAGGACAAAAACGAAGAGGCATTTTGCGTGAAGGTCA TCTACCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG216464 representing NM_015102
Red=Cloning site Green=Tags(s) MNDWHRIFTQNVLVPPHPQRARQPWKESTAFQCVLKWLDGPVIRQGVLEVLSEVECHLRVSFFDVTYRHF FGRTWKTTVKPTKRPPSRIVFNEPLYFHTSLNHPHIVAVVEVVAEGKKRDGSLQTLSCGFGILRIFSNQP DSPISASQDKRLRLYHGTPRALLHPLLQDPAEQNRHMTLIENCSLQYTLKPHPALEPAFHLLPENLLVSG LQQIPGLLPAHGESGDALRKPRLQKPITGHLDDLFFTLYPSLEKFEEELLELHVQDHFQEGCGPLDGGAL EILERRLRVGVHNGLGFVQRPQVVVLVPEMDVALTRSASFSRKVVSSSKTSSGSQALVLRSRLRLPEMVG HPAFAVIFQLEYVFSSPAGVDGNAASVTSLSNLACMHMVRWAVWNPLLEADSGRVTLPLQGGIQPNPSHC LVYKVPSASMSSEEVKQVESGTLRFQFSLGSEEHLDAPTEPVSGPKVERRPSRKPPTSPSSPPAPVPRVL AAPQNSPVGPGLSISQLAASPRSPTQHCLARPTSQLPHGSQASPAQAQEFPLEAGISHLEADLSQTSLVL ETSIAEQLQELPFTPLHAPIVVGTQTRSSAGQPSRASMVLLQSSGFPEILDANKQPAEAVSATEPVTFNP QKEESDCLQSNEMVLQFLAFSRVAQDCRGTSWPKTVYFTFQFYRFPPATTPRLQLVQLDEAGQPSSGALT HILVPVSRDGTFDAGSPGFQLRYMVGPGFLKPGERRCFARYLAVQTLQIDVWDGDSLLLIGSAAVQMKHL LRQGRPAVQASHELEVVATEYEQDNMVVSGDMLGFGRVKPIGVHSVVKGRLHLTLANVGHPCEQKVRGCS TLPPSRSRVISNDGASRFSGGSLLTTGSSRRKHVVQAQKLADVDSELAAMLLTHARQGKGPQDVSRESDA TRRRKLERMRSVRLQEAGGDLGRRGTSVLAQQSVRTQHLRDLQVIAAYRERTKAESIASLLSLAITTEHT LHATLGVAEFFEFVLKNPHNTQHTVTVEIDNPELSVIVDSQEWRDFKGAAGLHTPVEEDMFHLRGSLAPQ LYLRPHETAHVPFKFQSFSAGQLAMVQASPGLSNEKGMDAVSPWKSSAVPTKHAKVLFRASGGKPIAVLC LTVELQPHVVDQVFRFYHPELSFLKKAIRLPPWHTFPGAPVGMLGEDPPVHVRCSDPNVICETQNVGPGE PRDIFLKVASGPSPEIKDFFVIIYSDRWLATPTQTWQVYLHSLQRVDVSCVAGQLTRLSLVLRGTQTVRK VRAFTSHPQELKTDPKGVFVLPPRGVQDLHVGVRPLRAGSRFVHLNLVDVDCHQLVASWLVCLCCRQPLI SKAFEIMLAAGEGKGVNKRITYTNPYPSRRTFHLHSDHPELLRFREDSFQVGGGETYTIGLQFAPSQRVG EEEILIYINDHEDKNEEAFCVKVIYQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015102 |
| ORF Size | 4278 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015102.5 |
| RefSeq Size | 5020 bp |
| RefSeq ORF | 4281 bp |
| Locus ID | 261734 |
| UniProt ID | O75161 |
| Gene Summary | This gene encodes a protein involved in renal tubular development and function. This protein interacts with nephrocystin, and belongs to a multifunctional complex that is localized to actin- and microtubule-based structures. Mutations in this gene are associated with nephronophthisis type 4, a renal disease, and with Senior-Loken syndrome type 4, a combination of nephronophthisis and retinitis pigmentosa. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216464 | NPHP4 (Myc-DDK-tagged)-Human nephronophthisis 4 (NPHP4) |
CNY 9,760.00 |
|
| RC216464L1 | Lenti-ORF clone of NPHP4 (Myc-DDK-tagged)-Human nephronophthisis 4 (NPHP4) |
CNY 12,160.00 |
|
| RC216464L2 | Lenti-ORF clone of NPHP4 (mGFP-tagged)-Human nephronophthisis 4 (NPHP4) |
CNY 12,730.00 |
|
| RC216464L3 | Lenti-ORF clone of NPHP4 (Myc-DDK-tagged)-Human nephronophthisis 4 (NPHP4) |
CNY 12,730.00 |
|
| RC216464L4 | Lenti-ORF clone of NPHP4 (mGFP-tagged)-Human nephronophthisis 4 (NPHP4) |
CNY 12,730.00 |
|
| SC304230 | NPHP4 (untagged)-Human nephronophthisis 4 (NPHP4) |
CNY 27,460.00 |
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