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MED13 (NM_005121) Human Tagged ORF Clone
CAT＃: RG216484
- TrueORF®
MED13 (tGFP-tagged) - Human mediator complex subunit 13 (MED13)
ORF Plasmid: DDK
"NM_005121" in other vectors (2)
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CNY 23,128.00
货期*
现货
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ARC250; DRIP250; HSPC221; MRD61; THRAP1; TRAP240 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG216484 representing NM_005121
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGCCTCCTTCGTGCCGAACGGGGCCAGCCTGGAAGATTGTCACTGTAACCTCTTCTGCCTGGCTG ACTTGACAGGAATTAAGTGGAAAAAATATGTATGGCAAGGCCCAACTTCTGCCCCTATTCTGTTTCCTGT GACAGAAGAAGACCCCATTTTGAGCAGTTTTAGTCGCTGCCTTAAGGCAGATGTACTTGGTGTTTGGCGG CGAGATCAAAGACCTGGAAGAAGAGAATTGTGGATATTTTGGTGGGGTGAAGACCCAGTTTTGCTGACCT TATTCACCATGACTTATCAGAAGAAGAAGATGGAATGTGGGAGAATGGACTTTCCTATGAATGCCGTACT CTGCTTTTCCAAAGCAGTTCACAATCTATTGGAACGGTGTTTAATGAACAGGAATTTTGTACGTATTGGC AAGTGGTTTGTAAAGCCTTATGAAAAAGATGAAAAACCTATAAATAAAAGTGAACACTTGTCCTGCTCCT TCACCTTTTTCTTGCATGGAGACAGCAATGTTTGTACCAGTGTGGAAATTAACCAACATCAACCTGTATA CCTTCTCAGTGAAGAGCATATCACCCTTGCTCAACAGTCTAATAGCCCATTTCAAGTTATCTTATGCCCA TTTGGACTAAATGGCACTCTCACAGGACAGGCATTCAAGATGTCTGATTCAGCTACAAAAAAATTAATTG GTGAATGGAAACAGTTCTATCCTATCTCATGTTGCTTGAAGGAGATGTCTGAAGAAAAACAGGAAGATAT GGATTGGGAAGATGATTCTTTAGCTGCAGTAGAAGTTCTTGTTGCTGGTGTCCGAATGATCTACCCAGCA TGCTTTGTTCTAGTCCCTCAGTCAGACATTCCTACTCCTAGCCCTGTGGGATCCACTCACTGTTCATCTT CTTGCTTGGGTGTCCACCAAGTGCCTGCTTCCACAAGAGATCCTGCTATGTCTTCGGTTACGCTTACACC ACCTACGTCTCCTGAGGAAGTCCAAACAGTTGATCCTCAGTCTGTCCAGAAGTGGGTCAAATTTTCTTCA GTATCTGATGGCTTCAACTCCGATAGTACTAGCCACCATGGTGGGAAAATACCCAGAAAATTAGCAAATC ATGTGGTGGATAGAGTTTGGCAAGAATGCAATATGAACAGAGCACAGAACAAGAAGAAGTATTCTGCTTC ATCAGGTGGTCTATGCGAAGAAGCGACAGCTGCTAAAGTGGCATCCTGGGATTTTGTTGAAGCCACACAA AGAACAAATTGCAGTTGTTTGAGGCACAAAAATCTCAAGTCAAGAAATGCTGGACAACAAGGACAGGCAC CATCTTTAGGTCAGCAACAACAAATACTTCCTAAGCACAAGACCAATGAGAAGCAAGAAAAGAGTGAAGA GCCACAGAAACGCCCCTTGACTCCTTTTCACCATCGTGTGTCTGTTAGTGATGATGTTGGCATGGACGCA GATTCAGCCAGCCAAAGACTTGTGATCTCTGCTCCAGACAGTCAAGTGAGATTTTCAAATATCCGAACTA ATGATGTAGCAAAGACTCCTCAGATGCATGGCACCGAAATGGCAAATTCACCTCAACCACCCCCACTTAG TCCTCACCCTTGTGATGTGGTTGATGAAGGAGTGACTAAAACACCTTCAACTCCTCAGAGTCAACATTTT TATCAAATGCCAACACCAGATCCCTTGGTTCCTTCTAAACCAATGGAAGATAGGATAGACAGTTTGTCCC AGTCTTTCCCACCTCAATATCAGGAAGCTGTAGAACCTACAGTATATGTTGGTACAGCAGTAAACTTGGA AGAAGATGAAGCCAATATAGCCTGGAAGTATTACAAGTTCCCAAAGAAAAAAGATGTAGAGTTTTTACCA CCTCAACTTCCAAGTGATAAATTCAAGGATGATCCAGTTGGACCTTTTGGACAGGAAAGTGTAACATCAG TTACAGAGTTAATGGTGCAATGTAAGAAACCTTTAAAAGTTTCTGATGAATTAGTGCAGCAATATCAAAT TAAAAACCAGTGTCTTTCAGCAATAGCATCTGATGCAGAACAAGAACCTAAAATTGATCCATATGCATTT GTTGAAGGAGATGAGGAATTCCTTTTTCCTGATAAAAAAGATAGACAAAATAGTGAGAGAGAAGCTGGAA AAAAACACAAGGTAGAAGATGGGACATCTAGTGTAACAGTGTTATCACATGAAGAAGATGCTATGTCATT ATTTAGTCCCTCTATCAAGCAAGATGCTCCACGCCCTACTAGTCATGCCCGTCCTCCATCAACAAGTTTG ATTTATGACTCAGACCTGGCTGTCTCTTATACTGACCTTGATAATCTCTTCAATTCTGATGAAGATGAAC TAACACCTGGATCTAAAAGATCAGCAAATGGATCAGATGATAAAGCCAGCTGCAAGGAATCAAAGACAGG AAATCTGGACCCGTTATCTTGCATAAGCACTGCAGATCTTCATAAAATGTATCCTACACCACCATCATTG GAACAACATATTATGGGATTTTCCCCAATGAATATGAATAATAAAGAATATGGTAGTATGGATACAACAC CTGGAGGAACTGTTCTAGAAGGAAATAGTTCTAGTATAGGAGCGCAGTTCAAAATTGAGGTTGATGAGGG ATTCTGTAGCCCCAAACCTTCTGAAATTAAAGATTTTTCTTATGTCTATAAGCCTGAAAATTGTCAAATT CTAGTGGGATGTTCCATGTTTGCACCTCTAAAAACTCTACCAAGCCAATATCTGCCCCTTATCAAATTGC CAGAAGAGTGTATTTACCGTCAGAGTTGGACTGTTGGAAAATTGGAATTGCTTTCTTCAGGGCCTTCAAT GCCATTCATCAAAGAGGGTGATGGAAGTAATATGGATCAAGAATATGGCACTGCTTATACACCTCAAACT CATACTTCTTGTGGGATGCCTCCTAGCAGTGCACCTCCTAGTAACAGCGGAGCAGGAATTCTTCCTTCTC CATCCACCCCTCGGTTTCCAACTCCAAGGACTCCAAGGACTCCTCGGACTCCTCGTGGAGCTGGTGGACC TGCTAGTGCTCAAGGTTCAGTCAAATATGAAAATTCAGACTTGTATTCACCAGCTTCTACCCCATCTACA TGCAGACCCCTTAATTCTGTTGAACCTGCAACTGTCCCTTCCATCCCTGAAGCACACAGTCTTTATGTAA ACCTCATCCTTTCAGAATCAGTTATGAATTTGTTTAAAGACTGTAACTCTGATAGTTGTTGCATCTGTGT TTGCAACATGAACATCAAGGGTGCCGATGTTGGAGTTTACATTCCAGATCCAACGCAGGAAGCACAATAT AGGTGTACCTGTGGCTTCAGTGCTGTCATGAACAGAAAATTTGGAAACAATTCAGGATTATTTCTTGAAG ATGAACTAGATATCATAGGACGCAATACAGACTGTGGCAAAGAAGCAGAAAAACGTTTTGAAGCTCTCAG GGCTACCTCTGCTGAACATGTTAATGGAGGACTAAAGGAATCTGAAAAATTATCTGATGATTTGATATTA TTGCTACAAGATCAGTGCACTAATTTATTTTCACCCTTTGGAGCAGCAGACCAAGATCCTTTTCCTAAAA GTGGTGTAATTAGCAATTGGGTACGTGTTGAAGAGCGTGACTGTTGCAATGACTGCTACCTTGCATTAGA ACATGGGCGTCAGTTCATGGATAACATGTCAGGAGGAAAAGTTGATGAAGCACTTGTGAAAAGTTCATGC TTACACCCCTGGTCCAAAAGAAACGATGTGAGTATGCAGTGCTCACAGGATATACTTCGAATGCTCCTCT CTCTTCAGCCAGTTCTTCAGGATGCCATTCAGAAAAAAAGAACAGTAAGACCTTGGGGTGTTCAGGGTCC TCTCACTTGGCAACAATTTCATAAAATGGCTGGCCGAGGCTCTTATGGAACTGATGAATCCCCAGAACCA CTGCCAATCCCCACATTTTTGTTGGGTTATGATTATGATTATCTGGTGCTTTCTCCATTTGCTCTTCCTT ATTGGGAGAGACTTATGCTGGAACCCTATGGATCTCAAAGAGATATAGCCTATGTTGTACTGTGTCCAGA AAATGAAGCCTTGTTAAATGGAGCAAAAAGCTTTTTTAGAGATCTTACTGCAATATATGAGTCCTGTCGA TTAGGTCAACATAGACCTGTTTCTCGACTGTTAACAGATGGGATCATGAGAGTTGGATCTACTGCATCAA AGAAACTATCAGAAAAGTTGGTAGCAGAATGGTTTTCTCAGGCAGCTGATGGTAACAATGAAGCATTTTC TAAACTCAAGCTTTATGCACAAGTCTGCAGATATGACCTAGGTCCTTATCTTGCTTCCCTGCCATTGGAC AGCTCTCTACTTTCCCAGCCAAATTTAGTTGCCCCTACAAGTCAGTCTTTGATTACTCCACCTCAGATGA CAAATACTGGAAATGCTAATACTCCATCTGCCACCTTAGCATCTGCAGCGAGCAGCACTATGACAGTGAC TTCAGGTGTTGCCATATCTACTTCAGTTGCCACAGCTAATTCAACTTTGACCACAGCTTCAACTTCATCT TCATCATCCTCCAACTTGAATAGTGGAGTATCATCAAATAAACTACCTTCGTTTCCACCCTTTGGCAGTA TGAACAGTAATGCTGCAGGATCCATGTCTACACAAGCAAATACAGTTCAGAGTGGTCAGCTAGGAGGGCA ACAGACATCAGCTCTACAGACAGCTGGGATTTCTGGAGAATCATCTTCACTTCCCACTCAGCCGCATCCT GATGTGTCTGAAAGCACGATGGATCGGGATAAAGTGGGAATCCCCACAGATGGTGATTCACATGCAGTCA CGTATCCACCTGCAATTGTTGTTTATATAATTGATCCTTTTACATACGAAAATACAGACGAGAGCACTAA CTCTTCTAGTGTGTGGACATTGGGGCTACTTCGATGCTTTCTAGAAATGGTCCAGACTCTTCCTCCTCAT ATCAAGAGTACTGTTTCTGTACAGATTATTCCTTGTCAGTACCTGTTGCAACCTGTGAAGCATGAAGATA GAGAAATCTATCCCCAGCATTTAAAATCCCTGGCTTTTTCGGCCTTTACCCAGTGTCGGAGGCCACTTCC AACATCAACCAATGTGAAAACATTGACTGGCTTTGGTCCAGGTTTAGCCATGGAAACTGCCCTTAGAAGT CCTGATAGACCAGAGTGTATTCGACTTTATGCACCTCCTTTTATTCTGGCTCCAGTGAAGGACAAACAGA CAGAGCTAGGAGAAACATTTGGAGAAGCTGGACAGAAATATAATGTTCTTTTTGTGGGATACTGTTTATC ACATGATCAAAGGTGGATTCTTGCATCTTGCACAGATCTATATGGAGAACTTTTAGAAACTTGTATCATT AACATCGATGTTCCAAATAGGGCTCGTCGGAAAAAAAGTTCTGCTAGAAAATTTGGTCTACAGAAACTTT GGGAGTGGTGCTTAGGACTTGTACAAATGAGTTCATTGCCATGGAGAGTTGTAATTGGTCGTCTAGGAAG GATTGGTCATGGAGAATTGAAAGATTGGAGCTGTTTGCTGAGTCGTCGAAACTTGCAGTCTCTAAGTAAA AGGCTCAAAGACATGTGTAGAATGTGTGGTATATCTGCTGCAGACTCCCCTAGCATTCTCAGTGCTTGCT TGGTGGCAATGGAGCCGCAAGGCTCTTTTGTTATTATGCCAGATTCTGTGTCAACTGGTTCTGTATTTGG AAGAAGCACGACTCTAAATATGCAGACATCTCAGCTAAATACCCCACAGGATACATCATGTACTCATATA CTTGTGTTTCCTACTTCTGCTTCTGTGCAAGTAGCTTCAGCTACTTATACCACTGAAAATTTGGATTTAG CTTTCAATCCCAACAATGATGGAGCAGATGGAATGGGTATCTTTGATTTGTTAGACACAGGAGATGATCT TGACCCTGATATCATTAATATCCTTCCTGCTTCTCCAACTGGTTCTCCTGTACATTCTCCAGGATCTCAT TACCCCCATGGAGGTGATGCGGGCAAGGGTCAGAGTACTGATCGGCTACTATCAACAGAACCTCATGAGG AAGTACCTAATATTCTTCAGCAACCATTGGCCCTTGGTTACTTTGTATCAACTGCCAAAGCAGGTCCATT ACCTGACTGGTTCTGGTCAGCATGTCCTCAAGCACAATATCAGTGTCCCCTTTTTCTTAAGGCCTCTTTG CACCTCCACGTGCCTTCAGTGCAATCTGACGAGCTGCTTCACAGTAAACACTCCCACCCACTTGACTCAA ATCAGACTTCAGATGTCCTCAGGTTTGTTTTGGAACAGTACAATGCACTCTCCTGGCTAACCTGTGACCC TGCAACCCAGGACAGACGCTCATGTCTCCCAATTCATTTTGTGGTGCTGAATCAGTTATATAACTTTATT ATGAATATGCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG216484 representing NM_005121
Red=Cloning site Green=Tags(s) MSASFVPNGASLEDCHCNLFCLADLTGIKWKKYVWQGPTSAPILFPVTEEDPILSSFSRCLKADVLGVWR RDQRPGRRELWIFWWGEDPVLLTLFTMTYQKKKMECGRMDFPMNAVLCFSKAVHNLLERCLMNRNFVRIG KWFVKPYEKDEKPINKSEHLSCSFTFFLHGDSNVCTSVEINQHQPVYLLSEEHITLAQQSNSPFQVILCP FGLNGTLTGQAFKMSDSATKKLIGEWKQFYPISCCLKEMSEEKQEDMDWEDDSLAAVEVLVAGVRMIYPA CFVLVPQSDIPTPSPVGSTHCSSSCLGVHQVPASTRDPAMSSVTLTPPTSPEEVQTVDPQSVQKWVKFSS VSDGFNSDSTSHHGGKIPRKLANHVVDRVWQECNMNRAQNKKKYSASSGGLCEEATAAKVASWDFVEATQ RTNCSCLRHKNLKSRNAGQQGQAPSLGQQQQILPKHKTNEKQEKSEEPQKRPLTPFHHRVSVSDDVGMDA DSASQRLVISAPDSQVRFSNIRTNDVAKTPQMHGTEMANSPQPPPLSPHPCDVVDEGVTKTPSTPQSQHF YQMPTPDPLVPSKPMEDRIDSLSQSFPPQYQEAVEPTVYVGTAVNLEEDEANIAWKYYKFPKKKDVEFLP PQLPSDKFKDDPVGPFGQESVTSVTELMVQCKKPLKVSDELVQQYQIKNQCLSAIASDAEQEPKIDPYAF VEGDEEFLFPDKKDRQNSEREAGKKHKVEDGTSSVTVLSHEEDAMSLFSPSIKQDAPRPTSHARPPSTSL IYDSDLAVSYTDLDNLFNSDEDELTPGSKRSANGSDDKASCKESKTGNLDPLSCISTADLHKMYPTPPSL EQHIMGFSPMNMNNKEYGSMDTTPGGTVLEGNSSSIGAQFKIEVDEGFCSPKPSEIKDFSYVYKPENCQI LVGCSMFAPLKTLPSQYLPLIKLPEECIYRQSWTVGKLELLSSGPSMPFIKEGDGSNMDQEYGTAYTPQT HTSCGMPPSSAPPSNSGAGILPSPSTPRFPTPRTPRTPRTPRGAGGPASAQGSVKYENSDLYSPASTPST CRPLNSVEPATVPSIPEAHSLYVNLILSESVMNLFKDCNSDSCCICVCNMNIKGADVGVYIPDPTQEAQY RCTCGFSAVMNRKFGNNSGLFLEDELDIIGRNTDCGKEAEKRFEALRATSAEHVNGGLKESEKLSDDLIL LLQDQCTNLFSPFGAADQDPFPKSGVISNWVRVEERDCCNDCYLALEHGRQFMDNMSGGKVDEALVKSSC LHPWSKRNDVSMQCSQDILRMLLSLQPVLQDAIQKKRTVRPWGVQGPLTWQQFHKMAGRGSYGTDESPEP LPIPTFLLGYDYDYLVLSPFALPYWERLMLEPYGSQRDIAYVVLCPENEALLNGAKSFFRDLTAIYESCR LGQHRPVSRLLTDGIMRVGSTASKKLSEKLVAEWFSQAADGNNEAFSKLKLYAQVCRYDLGPYLASLPLD SSLLSQPNLVAPTSQSLITPPQMTNTGNANTPSATLASAASSTMTVTSGVAISTSVATANSTLTTASTSS SSSSNLNSGVSSNKLPSFPPFGSMNSNAAGSMSTQANTVQSGQLGGQQTSALQTAGISGESSSLPTQPHP DVSESTMDRDKVGIPTDGDSHAVTYPPAIVVYIIDPFTYENTDESTNSSSVWTLGLLRCFLEMVQTLPPH IKSTVSVQIIPCQYLLQPVKHEDREIYPQHLKSLAFSAFTQCRRPLPTSTNVKTLTGFGPGLAMETALRS PDRPECIRLYAPPFILAPVKDKQTELGETFGEAGQKYNVLFVGYCLSHDQRWILASCTDLYGELLETCII NIDVPNRARRKKSSARKFGLQKLWEWCLGLVQMSSLPWRVVIGRLGRIGHGELKDWSCLLSRRNLQSLSK RLKDMCRMCGISAADSPSILSACLVAMEPQGSFVIMPDSVSTGSVFGRSTTLNMQTSQLNTPQDTSCTHI LVFPTSASVQVASATYTTENLDLAFNPNNDGADGMGIFDLLDTGDDLDPDIINILPASPTGSPVHSPGSH YPHGGDAGKGQSTDRLLSTEPHEEVPNILQQPLALGYFVSTAKAGPLPDWFWSACPQAQYQCPLFLKASL HLHVPSVQSDELLHSKHSHPLDSNQTSDVLRFVLEQYNALSWLTCDPATQDRRSCLPIHFVVLNQLYNFI MNML TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005121 |
| ORF Size | 6522 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005121.1, NP_005112.1 |
| RefSeq Size | 10474 bp |
| RefSeq ORF | 6525 bp |
| Locus ID | 9969 |
| UniProt ID | Q9UHV7 |
| Protein Families | Druggable Genome, Transcription Factors |
| Gene Summary | This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. The product of this gene is proposed to form a sub-complex with MED12, cyclin C, and CDK8 that can negatively regulate transactivation by mediator. [provided by RefSeq, Jul 2008] |
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