Epac2 (RAPGEF4) (NM_001100397) Human Tagged ORF Clone
CAT#: RG217088
- TrueORF®
RAPGEF4 (tGFP-tagged) - Human Rap guanine nucleotide exchange factor (GEF) 4 (RAPGEF4), transcript variant 2
ORF Plasmid: DDK
"NM_001100397" in other vectors (4)
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CNY 4,370.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | CAMP-GEFII; CGEF2; EPAC; EPAC 2; EPAC2; Nbla00496 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG217088 representing NM_001100397
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCTACAAGAAATATCGACAGTATATGGCAGGACTTCTGGCTCCTCCTTATGGTGTTATGGAAACGG GCTCTAACAATGACAGGATTCCTGACAAGGAGAACACACCTCTCATTGAACCTCACGTTCCTCTTCGTCC TGCTAACACCATTACCAAGGTCCCTTCAGAGAAGATCCTCAGAGCTGGAAAAATTTTACGAAATGCCATT CTCTCTCGAGCACCTCACATGATAAGAGATAGAAAATACCACCTAAAGACATACAGACAATGCTGTGTGG GAACTGAACTGGTGGACTGGATGATGCAGCAGACACCATGTGTTCACTCCCGGACTCAAGCTGTTGGCAT GTGGCAAGTCCTGTTAGAAGATGGTGTTCTCAACCACGTGGACCAGGAGCACCATTTCCAAGACAAATAT TTATTCTATCGATTTCTGGATGATGAGCACGAGGATGCCCCTTTGCCTACTGAGGAGGAGAAGAAGGAGT GTGATGAGGAGCTCCAGGACACCATGCTGCTGCTGTCACAGATGGGCCCCGACGCCCACATGAGGATGAT CCTTCGCAAACCACCTGGCCAGAGGACTGTGGATGACCTAGAGATTATCTATGAGGAGCTTCTTCATATT AAAGCCTTATCCCATCTTTCTACCACAGTGAAACGAGAGTTAGCAGGTGTTCTCATTTTTGAGTCTCACG CCAAAGGAGGGACTGTGTTGTTTAACCAGGGGGAAGAAGGTACCTCCTGGTACATTATTCTAAAAGGATC AGTGAATGTAGTCATTTACGGCAAGGGTGTGGTCTGCACCCTGCATGAAGGAGATGACTTCGGCAAGTTA GCACTAGTGAATGATGCCCCACGAGCTGCCTCTATCGTCTTACGAGAAGATAACTGCCATTTCTTAAGAG TAGACAAGGAGGATTTCAACCGGATCCTAAGGGACGTGGAGGCGAATACAGTCAGACTTAAAGAACATGA CCAAGATGTCTTGGTGCTGGAGAAGGTCCCAGCAGGGAACAGAGCTTCTAATCAAGGAAACTCACAGCCT CAGCAAAAGTATACTGTGATGTCAGGAACACCTGAAAAAATTTTAGAGCATTTTCTAGAAACAATACGCC TTGAGGCAACTTTAAATGAAGCAACAGATTCTGTTTTAAATGACTTTATTATGATGCACTGTGTTTTTAT GCCAAATACCCAGCTTTGCCCGGCACTGGTGGCCCACTACCACGCACAGCCTTCACAAGGTACAGAACAG GAGAAAATGGATTATGCCCTCAACAATAAGAGGCGAGTCATCCGCCTGGTTCTACAGTGGGCTGCCATGT ATGGAGACCTCCTGCAAGAGGATGACGTGTCTATGGCCTTCCTGGAGGAGTTTTATGTATCTGTATCAGA TGATGCCCGGATGATTGCTGCCCTCAAGGAGCAACTGCCAGAGTTGGAGAAGATTGTCAAGCAAATCTCA GAAGATGCAAAGGCACCACAAAAGAAGCACAAGGTTCTTTTGCAACAGTTCAATACGGGCGATGAGAGAG CCCAGAAGCGCCAGCCTATCCGCGGCTCTGATGAAGTTCTGTTTAAGGTCTATTGCATGGACCACACCTA CACAACCATTCGGGTGCCAGTGGCCACTTCGGTGAAGGAAGTCATCAGTGCAGTTGCCGACAAGCTGGGC TCCGGGGAGGGCCTGATCATAGTCAAGATGAGTTCCGGAGGAGAAAAGGTGGTGCTCAAACCTAATGATG TTTCAGTATTTACGACGCTCACCATTAATGGACGCCTGTTTGCTTGCCCGCGAGAGCAATTCGATTCACT GACTCCCTTACCAGAACAGGAAGGCCCAACTGTTGGAACAGTGGGAACTTTTGAACTGATGAGCTCCAAA GATTTAGCATACCAGATGACAATTTATGATTGGGAACTCTTCAACTGCGTGCATGAGCTGGAGCTAATCT ATCACACATTTGGAAGGCATAATTTTAAAAAGACCACAGCAAACTTGGATTTGTTCCTGAGGAGATTTAA TGAAATTCAGTTTTGGGTCGTCACTGAGATCTGCCTTTGTTCTCAGCTCAGCAAGCGTGTTCAGCTATTA AAAAAATTTATTAAGATAGCAGCCCACTGTAAGGAGTATAAAAATCTGAATTCCTTTTTTGCCATCGTCA TGGGACTAAGTAACGTTGCTGTGAGCCGCTTGGCACTAACGTGGGAGAAACTGCCAAGCAAGTTCAAGAA GTTCTATGCGGAGTTTGAAAGTTTAATGGACCCTTCAAGGAACCACAGGGCCTACAGGCTGACAGTAGCT AAGCTGGAACCTCCTCTCATCCCCTTCATGCCTTTGCTCATTAAAGATATGACATTTACTCATGAGGGGA ACAAGACGTTCATTGACAATCTAGTAAACTTTGAAAAAATGCGCATGATTGCAAATACGGCCAGAACAGT GAGATACTACAGGAGCCAACCCTTCAATCCTGATGCAGCTCAAGCTAATAAGAACCATCAGGATGTCCGG AGTTATGTACGGCAATTAAATGTGATTGACAACCAGAGAACTTTATCACAGATGTCACACAGATTAGAGC CTCGTCGACCA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG217088 representing NM_001100397
Red=Cloning site Green=Tags(s) MLYKKYRQYMAGLLAPPYGVMETGSNNDRIPDKENTPLIEPHVPLRPANTITKVPSEKILRAGKILRNAI LSRAPHMIRDRKYHLKTYRQCCVGTELVDWMMQQTPCVHSRTQAVGMWQVLLEDGVLNHVDQEHHFQDKY LFYRFLDDEHEDAPLPTEEEKKECDEELQDTMLLLSQMGPDAHMRMILRKPPGQRTVDDLEIIYEELLHI KALSHLSTTVKRELAGVLIFESHAKGGTVLFNQGEEGTSWYIILKGSVNVVIYGKGVVCTLHEGDDFGKL ALVNDAPRAASIVLREDNCHFLRVDKEDFNRILRDVEANTVRLKEHDQDVLVLEKVPAGNRASNQGNSQP QQKYTVMSGTPEKILEHFLETIRLEATLNEATDSVLNDFIMMHCVFMPNTQLCPALVAHYHAQPSQGTEQ EKMDYALNNKRRVIRLVLQWAAMYGDLLQEDDVSMAFLEEFYVSVSDDARMIAALKEQLPELEKIVKQIS EDAKAPQKKHKVLLQQFNTGDERAQKRQPIRGSDEVLFKVYCMDHTYTTIRVPVATSVKEVISAVADKLG SGEGLIIVKMSSGGEKVVLKPNDVSVFTTLTINGRLFACPREQFDSLTPLPEQEGPTVGTVGTFELMSSK DLAYQMTIYDWELFNCVHELELIYHTFGRHNFKKTTANLDLFLRRFNEIQFWVVTEICLCSQLSKRVQLL KKFIKIAAHCKEYKNLNSFFAIVMGLSNVAVSRLALTWEKLPSKFKKFYAEFESLMDPSRNHRAYRLTVA KLEPPLIPFMPLLIKDMTFTHEGNKTFIDNLVNFEKMRMIANTARTVRYYRSQPFNPDAAQANKNHQDVR SYVRQLNVIDNQRTLSQMSHRLEPRRP TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001100397 |
ORF Size | 2601 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001100397.2 |
RefSeq Size | 4120 bp |
RefSeq ORF | 2604 bp |
Locus ID | 11069 |
UniProt ID | Q8WZA2 |
Protein Pathways | Leukocyte transendothelial migration |
Gene Summary | Guanine nucleotide exchange factor (GEF) for RAP1A, RAP1B and RAP2A small GTPases that is activated by binding cAMP. Seems not to activate RAB3A. Involved in cAMP-dependent, PKA-independent exocytosis through interaction with RIMS2 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC217088 | RAPGEF4 (Myc-DDK-tagged)-Human Rap guanine nucleotide exchange factor (GEF) 4 (RAPGEF4), transcript variant 2 |
CNY 3,990.00 |
|
RC217088L3 | Lenti-ORF clone of RAPGEF4 (Myc-DDK-tagged)-Human Rap guanine nucleotide exchange factor (GEF) 4 (RAPGEF4), transcript variant 2 |
CNY 5,890.00 |
|
RC217088L4 | Lenti-ORF clone of RAPGEF4 (mGFP-tagged)-Human Rap guanine nucleotide exchange factor (GEF) 4 (RAPGEF4), transcript variant 2 |
CNY 5,890.00 |
|
SC316841 | RAPGEF4 (untagged)-Human Rap guanine nucleotide exchange factor (GEF) 4 (RAPGEF4), transcript variant 2 |
CNY 7,220.00 |