POFUT2 (NM_015227) Human Tagged ORF Clone
CAT#: RG217107
- TrueORF®
POFUT2 (tGFP-tagged) - Human protein O-fucosyltransferase 2 (POFUT2), transcript variant 1
ORF Plasmid: DDK
"NM_015227" in other vectors (4)
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CNY 4,370.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | C21orf80; FUT13 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG217107 representing NM_015227
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACACTCAGCTTCGTCTTCCTGCTGCTGGGGGCAGTGTCCTGGCCTCCGGCTTCTGCCTCCGGCC AGGAGTTCTGGCCCGGACAATCGGCGGCCGATATTCTGTCGGGGGCGGCTTCCCGCAGACGGTATCTTCT GTATGACGTCAACCCCCCGGAAGGCTTCAACCTGCGCAGGGATGTCTATATCCGAATCGCCTCTCTCCTG AAGACTCTGCTGAAGACGGAGGAGTGGGTGCTTGTCCTGCCTCCATGGGGCCGCCTCTATCACTGGCAGA GTCCTGACATCCACCAGGTCCGGATTCCCTGGTCTGAGTTTTTTGATCTTCCAAGTCTCAATAAAAACAT CCCCGTCATCGAGTATGAGCAGTTCATCGCAGAATCTGGTGGGCCCTTTATTGACCAGGTTTACGTCCTG CAAAGTTACGCAGAGGGGTGGAAAGAAGGGACCTGGGAAGAGAAGGTGGACGAGCGGCCGTGTATTGATC AGCTCCTGTACTCCCAGGACAAGCACGAGTACTACAGAGGATGGTTTTGGGGTTATGAGGAGACCAGGGG TCTAAACGTCTCCTGTCTGTCCGTCCAGGGCTCAGCCTCCATCGTGGCGCCCCTGCTGCTGAGAAACACA TCAGCCCGGTCCGTGATGTTAGACAGAGCCGAGAACCTACTTCACGACCACTATGGAGGGAAAGAATACT GGGATACCCGTCGCAGCATGGTGTTTGCCAGGCACCTGCGGGAGGTGGGAGACGAGTTCAGGAGCAGACA TCTCAACTCCACGGACGACGCAGACAGGATCCCCTTCCAGGAGGACTGGATGAAGATGAAGGTCAAGCTG GGCTCCGCGCTAGGGGGCCCCTACCTGGGAGTCCACCTGAGAAGAAAAGATTTCATCTGGGGTCACAGAC AGGATGTACCCAGTCTGGAAGGGGCCGTGAGGAAGATCCGCAGCCTCATGAAGACCCACCGGCTGGACAA GGTGTTTGTGGCCACAGATGCCGTCAGAAAGGAATATGAAGAGCTAAAAAAGCTGTTACCCGAGATGGTG AGGTTTGAACCCACGTGGGAGGAGCTGGAGCTCTACAAGGACGGAGGCGTTGCGATTATTGACCAGTGGA TCTGCGCACACGCCAGGTGCCTGCCCACGTCACTGTCGGCCGAGAGCGGGTCGGGTGGCTTTCAAAGGTT CTTCTGTCCCAAGTACTCGGTGTCAGAGCAGATGGTCGCCTGTGTTCACAGTGGTCATTTCCATACTGTT TGCCTCCTCGTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG217107 representing NM_015227
Red=Cloning site Green=Tags(s) MATLSFVFLLLGAVSWPPASASGQEFWPGQSAADILSGAASRRRYLLYDVNPPEGFNLRRDVYIRIASLL KTLLKTEEWVLVLPPWGRLYHWQSPDIHQVRIPWSEFFDLPSLNKNIPVIEYEQFIAESGGPFIDQVYVL QSYAEGWKEGTWEEKVDERPCIDQLLYSQDKHEYYRGWFWGYEETRGLNVSCLSVQGSASIVAPLLLRNT SARSVMLDRAENLLHDHYGGKEYWDTRRSMVFARHLREVGDEFRSRHLNSTDDADRIPFQEDWMKMKVKL GSALGGPYLGVHLRRKDFIWGHRQDVPSLEGAVRKIRSLMKTHRLDKVFVATDAVRKEYEELKKLLPEMV RFEPTWEELELYKDGGVAIIDQWICAHARCLPTSLSAESGSGGFQRFFCPKYSVSEQMVACVHSGHFHTV CLLV TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_015227 |
ORF Size | 1272 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_015227.6 |
RefSeq Size | 4828 bp |
RefSeq ORF | 1275 bp |
Locus ID | 23275 |
UniProt ID | Q9Y2G5 |
Gene Summary | Fucose is typically found as a terminal modification of branched chain glycoconjugates, but it also exists in direct O-linkage to serine or threonine residues within cystine knot motifs in epidermal growth factor (EGF; MIM 131530)-like repeats or thrombospondin (THBS; see MIM 188060) type-1 repeats. POFUT2 is an O-fucosyltransferase that use THBS type-1 repeats as substrates (Luo et al., 2006 [PubMed 16464857]).[supplied by OMIM, Mar 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC217107 | POFUT2 (Myc-DDK-tagged)-Human protein O-fucosyltransferase 2 (POFUT2), transcript variant 1 |
CNY 3,656.00 |
|
RC217107L3 | Lenti-ORF clone of POFUT2 (Myc-DDK-tagged)-Human protein O-fucosyltransferase 2 (POFUT2), transcript variant 1 |
CNY 5,890.00 |
|
RC217107L4 | Lenti-ORF clone of POFUT2 (mGFP-tagged)-Human protein O-fucosyltransferase 2 (POFUT2), transcript variant 1 |
CNY 5,890.00 |
|
SC313401 | POFUT2 (untagged)-Human protein O-fucosyltransferase 2 (POFUT2), transcript variant 1 |
CNY 3,656.00 |