Amino terminal enhancer of split (AES) (NM_198969) Human Tagged ORF Clone
CAT#: RG217378
- TrueORF®
AES (tGFP-tagged) - Human amino-terminal enhancer of split (AES), transcript variant 1
ORF Plasmid: DDK
"NM_198969" in other vectors (4)
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CNY 4,370.00
Cited in 1 publication. |
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AES; AES-1; AES-2; ESP1; GRG; Grg-5; GRG5 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG217378 representing NM_198969
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGTCACAAGAATGGCTTCCCTCAGGAAGGCGGCATTACCGCTGCCTTTCTGCAGAAAAGGAAACTAA GGCTCAGCAAGAACCACCGCCCAGCCAGAGCCAAGGTCACAGAGCACGTCCGTGGCACGCGTCCAGGTCG TGCCACAGCAGGGCCGGCGGCTTCGACGCGGGCAGCCGGGTCCCTTTTCTTTGACAGATGGGGAAACCGA GGCCCGGCCGGCTGCCGGGGCTCCTCGCACCTACCCCAGCAACTCAAATTCACCACCTCGGACTCCTGCG ACCGCATCAAAGACGAATTTCAGCTACTGCAAGCTCAGTACCACAGCCTCAAGCTCGAATGTGACAAGTT GGCCAGTGAGAAGTCAGAGATGCAGCGTCACTATGTGATGTACTACGAGATGTCCTACGGCTTGAACATC GAGATGCACAAACAGGCTGAGATCGTCAAAAGGCTGAACGGGATTTGTGCCCAGGTCCTGCCCTACCTCT CCCAAGAGCACCAGCAGCAGGTCTTGGGAGCCATTGAGAGGGCCAAGCAGGTCACCGCTCCCGAGCTGAA CTCTATCATCCGACAGCAGCTCCAAGCCCACCAGCTGTCCCAGCTGCAGGCCCTGGCCCTGCCCTTGACC CCACTACCCGTGGGGCTGCAGCCGCCTTCGCTGCCGGCGGTCAGCGCAGGCACCGGCCTCCTCTCGCTGT CCGCGCTGGGTTCCCAGGCCCACCTCTCCAAGGAAGACAAGAACGGGCACGATGGTGACACCCACCAGGA GGATGATGGCGAGAAGTCGGAT AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG217378 representing NM_198969
Red=Cloning site Green=Tags(s) MCHKNGFPQEGGITAAFLQKRKLRLSKNHRPARAKVTEHVRGTRPGRATAGPAASTRAAGSLFFDRWGNR GPAGCRGSSHLPQQLKFTTSDSCDRIKDEFQLLQAQYHSLKLECDKLASEKSEMQRHYVMYYEMSYGLNI EMHKQAEIVKRLNGICAQVLPYLSQEHQQQVLGAIERAKQVTAPELNSIIRQQLQAHQLSQLQALALPLT PLPVGLQPPSLPAVSAGTGLLSLSALGSQAHLSKEDKNGHDGDTHQEDDGEKSD SGPTRTRRLE - GFP Tag - V |
Restriction Sites | SgfI-RsrII Cloning Scheme for this gene Plasmid Map |
ACCN | NM_198969 |
ORF Size | 792 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_198969.1, NP_945320.1 |
RefSeq Size | 1754 bp |
RefSeq ORF | 795 bp |
Locus ID | 166 |
UniProt ID | Q08117 |
Protein Families | Druggable Genome, Transcription Factors |
Gene Summary | The protein encoded by this gene is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The encoded protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Non‐enzymatic function of WRN RECQL helicase regulates removal of topoisomerase‐I‐DNA covalent complexes and triggers NF‐κB signaling in cancer). Non‐enzymatic function of WRN RECQL helicase regulates removal of topoisomerase‐I‐DNA covalent complexes and triggers NF‐κB signaling in cancer
,null,
Aging Cell
,PubMed ID 35582959
[TLE5]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC217378 | AES (Myc-DDK-tagged)-Human amino-terminal enhancer of split (AES), transcript variant 1 |
CNY 3,990.00 |
|
RC217378L3 | Lenti-ORF clone of AES (Myc-DDK-tagged)-Human amino-terminal enhancer of split (AES), transcript variant 1 |
CNY 5,890.00 |
|
RC217378L4 | Lenti-ORF clone of AES (mGFP-tagged)-Human amino-terminal enhancer of split (AES), transcript variant 1 |
CNY 5,890.00 |
|
SC307835 | AES (untagged)-Human amino-terminal enhancer of split (AES), transcript variant 1 |
CNY 3,990.00 |
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