PTP kappa (PTPRK) (NM_002844) Human Tagged ORF Clone
CAT#: RG217570
- TrueORF®
PTPRK (tGFP-tagged) - Human protein tyrosine phosphatase, receptor type, K (PTPRK), transcript variant 2
ORF Plasmid: DDK
"NM_002844" in other vectors (4)
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CNY 15,770.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | R-PTP-kappa |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG217570 representing NM_002844
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATACGACTGCGGCGGCGGCGCTGCCTGCTTTTGTGGCGCTCTTGCTCCTCTCTCCTTGGCCTCTCC TGGGATCGGCCCAAGGCCAGTTCTCCGCAGGTGGCTGTACTTTTGATGATGGTCCAGGGGCCTGTGATTA CCACCAGGATCTGTATGATGACTTTGAATGGGTGCATGTTAGTGCTCAAGAGCCTCATTATCTACCACCC GAGATGCCCCAAGGTTCCTATATGATAGTGGACTCTTCAGATCACGACCCTGGAGAAAAAGCCAGACTTC AGCTGCCTACAATGAAGGAGAACGACACTCACTGCATTGATTTCAGTTACCTATTATATAGCCAGAAAGG ACTGAATCCTGGCACTTTGAACATATTAGTTAGGGTGAATAAAGGACCTCTTGCCAATCCAATTTGGAAT GTGACTGGATTCACGGGTAGAGATTGGCTTCGGGCTGAGCTAGCAGTGAGCACCTTTTGGCCCAATGAAT ATCAGGTAATATTTGAAGCTGAAGTCTCAGGAGGGAGAAGTGGTTATATTGCCATTGATGACATCCAAGT ACTGAGTTATCCTTGTGATAAATCTCCTCATTTCCTCCGTCTAGGGGATGTAGAGGTGAATGCAGGGCAA AACGCTACATTTCAGTGCATTGCCACAGGGAGAGATGCTGTGCATAACAAGTTATGGCTCCAGAGACGAA ATGGAGAAGATATACCAGTAGCCCAGACTAAGAACATCAATCATAGAAGGTTTGCCGCTTCCTTCAGATT GCAAGAAGTGACAAAAACTGACCAGGATTTGTATCGCTGTGTAACTCAGTCAGAACGAGGTTCCGGTGTG TCCAATTTTGCTCAACTTATTGTGAGAGAACCGCCAAGACCCATTGCTCCTCCTCAGCTTCTTGGTGTTG GGCCTACATATTTGCTGATCCAACTAAATGCCAACTCGATCATTGGCGATGGTCCTATCATCCTGAAAGA AGTAGAGTACCGAATGACATCAGGATCCTGGACAGAAACCCATGCAGTCAATGCTCCAACTTACAAATTA TGGCATTTAGATCCAGATACCGAATATGAGATCCGAGTTCTACTTACAAGACCTGGTGAAGGTGGAACGG GGCTCCCAGGACCTCCACTAATCACCAGAACAAAATGTGCAGAACCTATGAGAACCCCAAAGACATTAAA GATTGCTGAAATACAGGCAAGACGGATTGCTGTGGACTGGGAATCCTTGGGTTACAACATTACGCGTTGC CACACTTTTAATGTCACTATCTGCTACCATTACTTCCGTGGTCACAACGAGAGCAAGGCAGACTGTTTGG ACATGGACCCCAAAGCCCCTCAGCATGTTGTGAACCATCTGCCACCTTATACAAATGTCAGCCTCAAGAT GATCCTAACCAATCCAGAGGGAAGGAAGGAGAGTGAAGAGACAATTATTCAAACTGATGAAGATGTGCCT GGTCCCGTACCAGTAAAATCTCTTCAAGGAACATCCTTTGAAAATAAGATCTTCTTGAACTGGAAAGAAC CTTTGGATCCAAATGGAATCATCACTCAATATGAGATCAGCTATAGCAGTATAAGATCATTTGATCCTGC AGTTCCAGTGGCTGGACCTCCCCAGACTGTATCAAATTTATGGAACAGTACACACCATGTCTTTATGCAT CTCCACCCTGGAACCACGTACCAGTTTTTCATAAGAGCCAGCACGGTCAAAGGCTTTGGTCCAGCCACAG CCATCAATGTCACCACCAATATCTCAGCTCCAACTTTACCTGACTATGAAGGAGTTGATGCCTCTCTCAA TGAAACTGCCACCACAATAACTGTATTGTTGAGACCAGCACAAGCCAAAGGTGCTCCTATCAGTGCTTAT CAGATTGTTGTGGAAGAACTGCACCCACACCGAACCAAGAGAGAAGCCGGAGCCATGGAATGCTACCAGG TTCCTGTCACATACCAAAATGCCATGAGTGGGGGTGCACCGTATTACTTTGCTGCAGAACTCCCCCCGGG AAACCTACCTGAGCCTGCCCCGTTCACTGTGGGTGACAATCGGACCTACCAAGGCTTTTGGAACCCTCCT TTGGCTCCGCGCAAAGGATACAACATCTATTTCCAGGCGATGAGCAGTGTGGAGAAGGAAACTAAAACCC AGTGCGTACGCATTGCTACAAAAGCAGCAGCAACAGAAGAACCAGAAGTGATCCCAGATCCCGCCAAGCA GACAGACAGAGTGGTGAAAATAGCAGGAATTAGTGCTGGAATTTTGGTGTTCATCCTCCTTCTCCTAGTT GTCATATTAATTGTAAAAAAGAGCAAACTTGCTAAAAAACGCAAAGATGCCATGGGGAATACCCGGCAGG AGATGACTCACATGGTGAATGCAATGGATCGAAGTTATGCTGATCAGAGCACTCTGCATGCAGAAGATCC TCTTTCCATCACCTTCATGGACCAACATAACTTTAGTCCAAGATATGAGAACCACAGTGCTACAGCAGAG TCCAGTCGCCTTCTAGACGTACCTCGCTACCTCTGTGAGGGGACGGAATCCCCTTACCAGACAGGACAGC TGCATCCAGCCATCAGGGTAGCTGATTTACTGCAGCACATTAATCTCATGAAGACATCAGACAGCTATGG GTTCAAAGAGGAATATGAGAGCTTTTTTGAAGGACAGTCAGCATCTTGGGATGTAGCTAAAAAAGATCAA AATAGAGCAAAAAACCGATATGGAAACATTATAGCATATGATCACTCCAGAGTGATTTTGCAACCCGTAG AGGATGATCCTTCCTCAGATTATATTAATGCCAACTATATTGATGGCTACCAGAGACCAAGTCATTACAT TGCAACCCAAGGTCCCGTTCATGAAACAGTGTATGATTTCTGGAGGATGATTTGGCAAGAACAATCTGCT TGCATTGTGATGGTTACAAATTTAGTTGAGGTTGGCCGGGTTAAATGCTATAAATATTGGCCTGATGATA CTGAAGTTTATGGTGACTTCAAAGTAACGTGTGTAGAAATGGAACCACTTGCTGAATATGTAGTTAGGAC ATTCACCCTGGAAAGGAGGGGGTACAATGAAATCCGTGAAGTTAAACAGTTCCATTTCACGGGCTGGCCT GACCATGGAGTGCCCTACCATGCTACAGGGCTGCTTTCCTTTATCCGGCGAGTCAAGTTATCAAACCCTC CCAGTGCTGGCCCCATCGTTGTACATTGCAGTGCTGGTGCTGGACGAACTGGCTGCTACATTGTGATTGA CATCATGCTAGACATGGCTGAAAGAGAGGGTGTTGTTGATATTTACAATTGTGTCAAAGCCTTAAGATCT CGGCGTATTAATATGGTCCAGACAGAGGAACAGTACATTTTTATTCATGATGCCATTTTAGAAGCCTGCT TATGTGGAGAAACTGCCATACCTGTCTGTGAATTTAAAGCTGCATATTTTGATATGATTAGAATAGACTC CCAGACTAACTCTTCACATCTCAAGGATGAATTTCAGACTCTGAATTCAGTCACCCCTCGACTACAAGCT GAAGACTGCAGTATAGCGTGCCTGCCAAGGAACCATGACAAGAACCGTTTCATGGACATGCTGCCACCTG ACAGATGTCTGCCTTTTTTAATTACAATTGATGGGGAGAGCAGTAACTACATCAATGCTGCTCTTATGGA CAGCTACAGGCAACCAGCTGCTTTCATCGTCACACAATACCCTCTGCCAAACACTGTAAAAGACTTCTGG AGATTAGTGTATGATTATGGCTGTACCTCCATTGTGATGTTAAACGAAGTCGACTTGTCCCAGGGCTGCC CTCAGTACTGGCCAGAGGAAGGGATGCTACGATATGGCCCCATCCAAGTGGAATGTATGTCTTGTTCAAT GGACTGTGATGTGATCAACCGGATTTTTAGGATATGCAATCTAACAAGACCACAGGAAGGTTATCTGATG GTGCAACAGTTTCAGTACCTAGGATGGGCTTCTCATCGAGAAGTGCCTGGATCCAAAAGGTCATTCTTGA AACTGATACTTCAGGTGGAAAAGTGGCAGGAGGAATGCGAGGAAGGGGAAGGCCGGACGATTATCCACTG CCTAAATGGTGGCGGGCGAAGTGGCATGTTCTGTGCTATAGGCATCGTTGTTGAAATGGTGAAACGGCAA AATGTTGTCGATGTTTTCCATGCAGTAAAGACACTGAGGAACAGCAAGCCAAACATGGTGGAAGCCCCGG AGCAATACCGTTTCTGCTATGATGTAGCTTTGGAGTACCTGGAATCATCT AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG217570 representing NM_002844
Red=Cloning site Green=Tags(s) MDTTAAAALPAFVALLLLSPWPLLGSAQGQFSAGGCTFDDGPGACDYHQDLYDDFEWVHVSAQEPHYLPP EMPQGSYMIVDSSDHDPGEKARLQLPTMKENDTHCIDFSYLLYSQKGLNPGTLNILVRVNKGPLANPIWN VTGFTGRDWLRAELAVSTFWPNEYQVIFEAEVSGGRSGYIAIDDIQVLSYPCDKSPHFLRLGDVEVNAGQ NATFQCIATGRDAVHNKLWLQRRNGEDIPVAQTKNINHRRFAASFRLQEVTKTDQDLYRCVTQSERGSGV SNFAQLIVREPPRPIAPPQLLGVGPTYLLIQLNANSIIGDGPIILKEVEYRMTSGSWTETHAVNAPTYKL WHLDPDTEYEIRVLLTRPGEGGTGLPGPPLITRTKCAEPMRTPKTLKIAEIQARRIAVDWESLGYNITRC HTFNVTICYHYFRGHNESKADCLDMDPKAPQHVVNHLPPYTNVSLKMILTNPEGRKESEETIIQTDEDVP GPVPVKSLQGTSFENKIFLNWKEPLDPNGIITQYEISYSSIRSFDPAVPVAGPPQTVSNLWNSTHHVFMH LHPGTTYQFFIRASTVKGFGPATAINVTTNISAPTLPDYEGVDASLNETATTITVLLRPAQAKGAPISAY QIVVEELHPHRTKREAGAMECYQVPVTYQNAMSGGAPYYFAAELPPGNLPEPAPFTVGDNRTYQGFWNPP LAPRKGYNIYFQAMSSVEKETKTQCVRIATKAAATEEPEVIPDPAKQTDRVVKIAGISAGILVFILLLLV VILIVKKSKLAKKRKDAMGNTRQEMTHMVNAMDRSYADQSTLHAEDPLSITFMDQHNFSPRYENHSATAE SSRLLDVPRYLCEGTESPYQTGQLHPAIRVADLLQHINLMKTSDSYGFKEEYESFFEGQSASWDVAKKDQ NRAKNRYGNIIAYDHSRVILQPVEDDPSSDYINANYIDGYQRPSHYIATQGPVHETVYDFWRMIWQEQSA CIVMVTNLVEVGRVKCYKYWPDDTEVYGDFKVTCVEMEPLAEYVVRTFTLERRGYNEIREVKQFHFTGWP DHGVPYHATGLLSFIRRVKLSNPPSAGPIVVHCSAGAGRTGCYIVIDIMLDMAEREGVVDIYNCVKALRS RRINMVQTEEQYIFIHDAILEACLCGETAIPVCEFKAAYFDMIRIDSQTNSSHLKDEFQTLNSVTPRLQA EDCSIACLPRNHDKNRFMDMLPPDRCLPFLITIDGESSNYINAALMDSYRQPAAFIVTQYPLPNTVKDFW RLVYDYGCTSIVMLNEVDLSQGCPQYWPEEGMLRYGPIQVECMSCSMDCDVINRIFRICNLTRPQEGYLM VQQFQYLGWASHREVPGSKRSFLKLILQVEKWQEECEEGEGRTIIHCLNGGGRSGMFCAIGIVVEMVKRQ NVVDVFHAVKTLRNSKPNMVEAPEQYRFCYDVALEYLESS SGPTRTRRLE - GFP Tag - V |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_002844 |
ORF Size | 4320 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002844.4 |
RefSeq Size | 6104 bp |
RefSeq ORF | 4323 bp |
Locus ID | 5796 |
UniProt ID | Q15262 |
Domains | Y_phosphatase, MAM, PTPc_motif, IG, FN3 |
Protein Families | Druggable Genome, Phosphatase, Transmembrane |
Gene Summary | The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem catalytic domains, and thus represents a receptor-type PTP. The extracellular region contains a meprin-A5 antigen-PTP mu (MAM) domain, an Ig-like domain and four fibronectin type III-like repeats. This PTP was shown to mediate homophilic intercellular interaction, possibly through the interaction with beta- and gamma-catenin at adherens junctions. Expression of this gene was found to be stimulated by TGF-beta 1, which may be important for the inhibition of keratinocyte proliferation. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC217570 | PTPRK (Myc-DDK-tagged)-Human protein tyrosine phosphatase, receptor type, K (PTPRK), transcript variant 2 |
CNY 14,350.00 |
|
RC217570L3 | Lenti-ORF clone of PTPRK (Myc-DDK-tagged)-Human protein tyrosine phosphatase, receptor type, K (PTPRK), transcript variant 2 |
CNY 16,250.00 |
|
RC217570L4 | Lenti-ORF clone of PTPRK (mGFP-tagged)-Human protein tyrosine phosphatase, receptor type, K (PTPRK), transcript variant 2 |
CNY 16,250.00 |
|
SC128011 | PTPRK (untagged)-Human protein tyrosine phosphatase, receptor type, K (PTPRK), transcript variant 2 |
CNY 9,864.00 |
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