PER3 (NM_016831) Human Tagged ORF Clone
CAT#: RG217739
- TrueORF®
PER3 (tGFP-tagged) - Human period homolog 3 (Drosophila) (PER3)
ORF Plasmid: DDK
"NM_016831" in other vectors (6)
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CNY 14,144.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | FASPS3; GIG13 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RG217739 representing NM_016831
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCGCGGGGAAGCTCCTGGCCCCGGGAGACGGGGGGCTAAGGACGAGGCCCTGGGCGAAGAATCGG GGGAGCGGTGGAGCCCCGAGTTCCATCTGCAGAGGAAATTGGCGGACAGCAGCCACAGTGAACAGCAAGA TCGAAACAGAGTTTCTGAAGAACTTATCATGGTTGTCCAAGAAATGAAAAAATACTTCCCCTCGGAGAGA CGCAATAAACCAAGCACTCTAGATGCCCTCAACTATGCTCTCCGCTGTGTCCACAGCGTTCAAGCAAACA GTGAGTTTTTCCAGATTCTCAGTCAGAATGGAGCACCTCAGGCAGATGTGAGCATGTACAGTCTTGAGGA GCTGGCCACTATCGCTTCAGAACACACTTCCAAAAACACAGATACCTTTGTGGCAGTATTTTCATTTCTG TCTGGAAGGTTAGTGCACATTTCTGAACAGGCTGCTTTGATCCTGAATCGTAAGAAAGATGTCCTGGCGT CTTCTCACTTTGTTGACCTGCTTGCACCTCAAGACATGAGGGTATTCTACGCGCACACTGCCAGAGCTCA GCTTCCTTTCTGGAACAACTGGACCCAAAGAGCTGCACGGTATGAATGTGCTCCGGTGAAACCTTTTTTC TGCAGGATCCGTGGAGGTGAAGACAGAAAGCAAGAGAAGTGTCACTCCCCATTCCGGATCATCCCCTATC TGATTCATGTACATCACCCTGCCCAGCCAGAATTGGAATCGGAACCTTGCTGTCTCACTGTGGTTGAAAA GATTCACTCTGGTTATGAAGCTCCTCGGATCCCAGTGAATAAAAGAATCTTCACCACCACACACACCCCA GGGTGTGTTTTTCTTGAAGTAGATGAAAAAGCAGTGCCTTTGCTGGGTTACCTACCTCAGGACCTGATTG GAACATCGATCCTAAGCTACCTGCACCCTGAAGATCGTTCTCTGATGGTTGCCATACACCAAAAAGTTTT GAAGTATGCAGGGCATCCTCCCTTTGAACATTCTCCCATTCGATTTTGTACTCAAAACGGAGACTACATC ATACTGGATTCCAGTTGGTCCAGCTTTGTGAATCCCTGGAGCCGGAAGATTTCTTTCATCATTGGTCGGC ATAAAGTTCGAACGAGCCCACTAAATGAGGATGTTTTTGCTACCAAAATTAAAAAGATGAACGATAATGA CAAAGACATAACAGAATTACAAGAACAAATTTACAAACTTCTCTTACAGCCAGTTCACGTGAGCGTGTCC AGCGGCTACGGGAGCCTGGGGAGCAGCGGGTCGCAGGAGCAGCTTGTCAGCATCGCCTCCTCCAGTGAGG CCAGTGGGCACCGTGTGGAGGAGACGAAGGCGGAGCAGATGACCTTGCAGCAGGTCTATGCCAGTGTGAA CAAAATTAAAAATCTGGGTCAGCAGCTCTACATTGAGTCAATGACCAAATCATCATTCAAGCCAGTGACG GGGACACGCACAGAACCGAATGGTGGTGGTGAATGTAAGACCTTTACTTCCTTCCACCAAACACTGAAAA ACAATAGTGTGTACACTGAGCCCTGTGAGGATTTGAGGAACGATGAGCACAGCCCATCCTATCAACAGAT CAACTGTATCGACAGTGTCATCAGATACCTGAAGAGCTACAACATTCCAGCTTTGAAAAGAAAGTGTATC TCCTGTACAAATACAACTTCTTCCTCCTCAGAAGAAGACAAACAGAACCACAAGGCAGATGATGTCCAAG CCTTACAAGCTGGTTTGCAAATCCCAGCCATACCTAAATCAGAAATGCCAACAAATGGACGGTCCATAGA CACAGGAGGAGGAGCTCCACAGATCCTGTCCACGGCGATGCTGAGCTTGGGGTCGGGCATAAGCCAATGC GGTTACAGCAGCACCATTGTCCATGTCCCACCCCCAGAGACAGCCAGGGATGCTACCCTCTTCTGTGAGC CCTGGACCCTGAACATGCAGCCAGCCCCTTTGACCTCGGAAGAATTTAAACACGTGGGGCTCACAGCGGC TGTTCTGTCAGCGCACACCCAGAAGGAAGAGCAGAATTATGTTGATAAATTCCGAGAAAAGATCCTGTCA TCACCCTACAGCTCCTATCTTCAGCAAGAAAGCAGGAGCAAAGCTAAATATTCATATTTTCAAGGAGATT CTACTTCCAAGCAGACGCGGTCGGCCGGCTGCAGGAAAGGGAAGCACAAGCGGAAGAAGCTGCCGGAGCC GCCAGACAGCAGCAGCTCGAACACCGGCTCTGGTCCCCGCAGGGGAGCGCATCAGAACGCACAGCCCTGC TGCCCCTCCGCGGCCTCCTCTCCGCACACCTCGAGCCCGACCTTCCCACCTGCCGCCATGGTGCCCAGCC AGGCCCCTTACCTCGTCCCAGCTTTTCCCCTCCCAGCCGCGACCTCACCCGGAAGAGAATACGCAGCCCC CGGAACTGCACCGGAAGGCCTGCATGGGCTGCCCTTGTCCGAGGGCTTGCAGCCTTACCCAGCTTTCCCT TTTCCTTACTTGGATACTTTTATGACCGTTTTCCTGCCTGACCCCCCTGTCTGTCCTCTGTTGTCGCCAT CGTTTTTGCCATGTCCATTCCTGGGGGCGACAGCCTCTTCTGCGATATCACCCTCAATGTCGTCAGCAAT GAGTCCAACTCTGGACCCACCCCCTTCAGTCACCAGCCAAAGGAGAGAGGAGGAAAAGTGGGAGGCACAA AGCGAGGGGCACCCGTTCATTACTTCGAGAAGCAGCTCACCCTTGCAGTTAAACTTACTTCAGGAAGAGA TGCCCAGACCCTCTGAATCTCCAGATCAGATGAGAAGGAACACGTGCCCACAAACTGAGTATTGTGTTAC AGGCAACAATGGCAGTGAGAGCAGTCCTGCTACTACCGGTGCACTGTCCACGGGGTCACCTCCCAGGGAG AATCCATCCCATCCTACTGCCAGCGCTCTGTCCACAGGATCGCCTCCCATGAAGAATCCATCCCATCCTA CTGCCAGCGCTCTGTCCACAGGATCGCCTCCCATGAAGAATCCATCCCATCCTACTGCCAGCACACTGTC CATGGGATTGCCTCCCAGCAGGACTCCATCCCATCCTACTGCCACTGTTCTGTCCACGGGGTCACCTCCC AGCGAATCCCCATCCAGAACTGGTTCAGCAGCATCAGGAAGCAGCGACAGCAGTATATACCTTACTAGTA GTGTTTATTCTTCTAAAATCTCCCAAAATGGGCAGCAATCTCAGGACGTACAGAAAAAAGAAACATTTCC TAATGTCGCCGAAGAGCCCATCTGGAGAATGATACGGCAGACACCTGAGCGCATTCTCATGACATACCAG GTACCTGAGAGGGTTAAAGAAGTTGTACTAAAAGAAGACCTGGAAAAGCTAGAAAGTATGAGGCAGCAGC AGCCCCAGTTTTCTCATGGGCAAAAGGAGGAGCTGGCTAAGGTGTATAATTGGATTCAAAGCCAGACTGT CACTCAAGAAATCGACATTCAAGCCTGTGTCACTTGTGAAAATGAAGATTCAGCTGATGGTGCGGCCACA TCCTGTGGTCAGGTTCTGGTAGAAGACAGCTGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG217739 representing NM_016831
Red=Cloning site Green=Tags(s) MPRGEAPGPGRRGAKDEALGEESGERWSPEFHLQRKLADSSHSEQQDRNRVSEELIMVVQEMKKYFPSER RNKPSTLDALNYALRCVHSVQANSEFFQILSQNGAPQADVSMYSLEELATIASEHTSKNTDTFVAVFSFL SGRLVHISEQAALILNRKKDVLASSHFVDLLAPQDMRVFYAHTARAQLPFWNNWTQRAARYECAPVKPFF CRIRGGEDRKQEKCHSPFRIIPYLIHVHHPAQPELESEPCCLTVVEKIHSGYEAPRIPVNKRIFTTTHTP GCVFLEVDEKAVPLLGYLPQDLIGTSILSYLHPEDRSLMVAIHQKVLKYAGHPPFEHSPIRFCTQNGDYI ILDSSWSSFVNPWSRKISFIIGRHKVRTSPLNEDVFATKIKKMNDNDKDITELQEQIYKLLLQPVHVSVS SGYGSLGSSGSQEQLVSIASSSEASGHRVEETKAEQMTLQQVYASVNKIKNLGQQLYIESMTKSSFKPVT GTRTEPNGGGECKTFTSFHQTLKNNSVYTEPCEDLRNDEHSPSYQQINCIDSVIRYLKSYNIPALKRKCI SCTNTTSSSSEEDKQNHKADDVQALQAGLQIPAIPKSEMPTNGRSIDTGGGAPQILSTAMLSLGSGISQC GYSSTIVHVPPPETARDATLFCEPWTLNMQPAPLTSEEFKHVGLTAAVLSAHTQKEEQNYVDKFREKILS SPYSSYLQQESRSKAKYSYFQGDSTSKQTRSAGCRKGKHKRKKLPEPPDSSSSNTGSGPRRGAHQNAQPC CPSAASSPHTSSPTFPPAAMVPSQAPYLVPAFPLPAATSPGREYAAPGTAPEGLHGLPLSEGLQPYPAFP FPYLDTFMTVFLPDPPVCPLLSPSFLPCPFLGATASSAISPSMSSAMSPTLDPPPSVTSQRREEEKWEAQ SEGHPFITSRSSSPLQLNLLQEEMPRPSESPDQMRRNTCPQTEYCVTGNNGSESSPATTGALSTGSPPRE NPSHPTASALSTGSPPMKNPSHPTASALSTGSPPMKNPSHPTASTLSMGLPPSRTPSHPTATVLSTGSPP SESPSRTGSAASGSSDSSIYLTSSVYSSKISQNGQQSQDVQKKETFPNVAEEPIWRMIRQTPERILMTYQ VPERVKEVVLKEDLEKLESMRQQQPQFSHGQKEELAKVYNWIQSQTVTQEIDIQACVTCENEDSADGAAT SCGQVLVEDSC TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_016831 |
ORF Size | 3603 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_016831.4 |
RefSeq Size | 6203 bp |
RefSeq ORF | 3606 bp |
Locus ID | 8863 |
UniProt ID | P56645 |
Domains | PAS |
Protein Families | Druggable Genome, Transcription Factors |
Protein Pathways | Circadian rhythm - mammal |
Gene Summary | This gene is a member of the Period family of genes and is expressed in a circadian pattern in the suprachiasmatic nucleus, the primary circadian pacemaker in the mammalian brain. Genes in this family encode components of the circadian rhythms of locomotor activity, metabolism, and behavior. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been linked to sleep disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC217739 | PER3 (Myc-DDK-tagged)-Human period homolog 3 (Drosophila) (PER3) |
CNY 12,544.00 |
|
RC217739L1 | Lenti-ORF clone of PER3 (Myc-DDK-tagged)-Human period homolog 3 (Drosophila) (PER3) |
CNY 14,944.00 |
|
RC217739L2 | Lenti-ORF clone of PER3 (mGFP-tagged)-Human period homolog 3 (Drosophila) (PER3) |
CNY 14,540.00 |
|
RC217739L3 | Lenti-ORF clone of PER3 (Myc-DDK-tagged)-Human period homolog 3 (Drosophila) (PER3) |
CNY 14,540.00 |
|
RC217739L4 | Lenti-ORF clone of PER3 (mGFP-tagged)-Human period homolog 3 (Drosophila) (PER3) |
CNY 14,540.00 |
|
SC309952 | PER3 (untagged)-Human period homolog 3 (Drosophila) (PER3) |
CNY 23,090.00 |