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SRGAP3 (NM_001033117) Human Tagged ORF Clone
CAT＃: RG217925
- TrueORF®
SRGAP3 (tGFP-tagged) - Human SLIT-ROBO Rho GTPase activating protein 3 (SRGAP3), transcript variant 2
ORF Plasmid: DDK
"NM_001033117" in other vectors (4)
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CNY 11,780.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ARHGAP14; MEGAP; SRGAP2; WRP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG217925 representing NM_001033117
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCATCTCAAACTAAGTTCAAGAAAGACAAAGAGATCATTGCTGAATATGAAGCCCAAATAAAAGAGA TCCGCACGCAGCTGGTGGAGCAGTTCAAATGTCTGGAGCAGCAATCAGAGTCGCGACTGCAGCTGCTTCA AGACCTCCAGGAGTTTTTCCGCCGGAAAGCTGAGATTGAGCTCGAGTACTCCCGCAGCCTGGAGAAGCTG GCTGAGCGCTTCTCCTCCAAAATCCGCAGCTCCCGGGAGCACCAGTTCAAGAAGGACCAGTACCTCCTCT CGCCTGTGAACTGTTGGTATCTGGTTCTGCATCAGACCCGGCGGGAGAGCCGAGACCATGCCACCCTCAA TGACATCTTCATGAACAATGTCATCGTCCGCCTCTCCCAGATCAGTGAGGATGTCATCAGACTCTTCAAA AAGAGCAAGGAGATTGGCCTGCAGATGCACGAGGAGCTCCTGAAGGTGACCAATGAGCTCTACACAGTCA TGAAAACCTACCACATGTACCATGCAGAGAGCATCAGTGCGGAAAGCAAGCTGAAGGAGGCTGAGAAGCA GGAGGAGAAGCAGTTCAATAAGTCAGGAGACCTCAGCATGAACCTGCTCCGGCACGAGGACCGGCCCCAG CGCCGCAGCTCTGTGAAGAAGATTGAGAAGATGAAGGAGAAGAGGCAGGCCAAGTACTCTGAGAACAAGC TGAAATGCACAAAGGCCCGGAATGACTACTTGCTCAATCTGGCAGCCACCAACGCAGCTATAAGCAAATA CTACATCCATGATGTCTCTGATCTGATCGATTGCTGTGATTTGGGCTTCCATGCCAGCCTGGCCCGCACC TTCCGGACCTATCTCTCAGCTGAATACAACCTGGAGACCTCTCGCCACGAAGGGCTGGATGTCATTGAGA ATGCAGTGGACAACCTGGATTCCCGAAGTGACAAGCACACAGTCATGGACATGTGCAATCAAGTCTTCTG CCCTCCACTCAAGTTCGAGTTCCAGCCCCACATGGGGGATGAGGTCTGCCAGGTCAGCGCTCAGCAGCCC GTCCAGACAGAACTGCTCATGCGTTATCACCAGCTGCAGTCCAGACTGGCCACCCTCAAGATAGAGAATG AGGAGGTTAGGAAAACCCTGGATGCCACCATGCAGACATTACAGGACATGCTGACTGTGGAGGACTTTGA TGTCTCCGATGCCTTCCAACACAGTCGATCGACAGAGTCCGTCAAGTCGGCTGCCTCTGAGACCTACATG AGCAAGATCAACATTGCCAAGAGGAGAGCCAACCAGCAGGAAACAGAAATGTTTTATTTTACAAAATTTA AAGAGTATGTGAATGGCAGTAACCTCATCACCAAGCTGCAGGCCAAGCACGATTTACTCAAGCAGACCCT GGGCGAAGGGGAAAGAGCAGAATGCGGCACCACCAGAGGAAGAAGAAATGCTCGAACCAGGAACCAGGAT TCAGGACAAGCTATACCGCTTGTAGTCGAGAGCTGCATCCGTTACATCAATTTATATGGACTCCAGCAGC AGGGGATCTTCAGAGTGCCAGGATCTCAGGTGGAAGTCAATGACATCAAAAATTCCTTTGAGAGAGGTGA AGACCCCCTTGTGGACGATCAAAATGAACGAGATATCAATTCAGTCGCTGGTGTTTTAAAACTGTATTTC CGAGGACTGGAAAACCCACTCTTTCCTAAGGAAAGGTTTCAAGATTTGATATCTACTATTAAACTGGAGA ACCCAGCCGAGAGGGTGCACCAGATCCAACAAATCCTCGTCACCCTTCCCCGCGTGGTCATTGTGGTCAT GAGATACCTCTTCGCTTTCCTCAACCACCTCTCCCAGTATAGCGACGAGAACATGATGGATCCCTACAAC CTGGCCATCTGCTTCGGGCCTACCCTCATGCACATCCCTGATGGGCAGGACCCTGTGTCCTGCCAGGCAC ACATCAATGAAGTCATCAAAACCATCATCATCCATCATGAAGCCATCTTCCCCAGCCCCCGGGAGCTAGA GGGACCTGTGTATGAAAAATGCATGGCTGGAGGGGAAGAATATTGTGACAGCCCACACAGCGAGCCAGGG GCCATCGATGAAGTTGACCATGACAATGGCACTGAGCCTCATACCAGCGATGAAGAAGTGGAGCAGATCG AGGCTATTGCCAAGTTTGACTACATGGGGCGGTCCCCGCGTGAGCTATCCTTCAAGAAGGGGGCCTCGCT GCTCCTGTACCACCGCGCCTCGGAGGACTGGTGGGAGGGCCGGCACAACGGCGTGGATGGACTCATCCCC CATCAGTACATAGTTGTACAGGACATGGATGATGCCTTCTCCGACAGCCTGAGCCAGAAGGCTGACAGCG AGGCCAGCAGTGGGCCATTGCTGGATGACAAGGCCTCTTCCAAAAACGACCTCCAGTCCCCCACGGAGCA CATCTCGGATTACGGCTTTGGGGGGGTGATGGGCCGAGTGCGGTTACGATCTGATGGAGCAGCCATCCCC AGACGCCGAAGCGGGGGCGACACACACAGCCCGCCCCGGGGCCTGGGCCCCAGCATAGACACACCACCCC GGGCTGCTGCCTGCCCCAGCAGCCCCCACAAAATCCCCCTCACCCGGGGGAGGATCGAGAGCCCTGAGAA GCGGAGGATGGCGACGTTCGGGAGCGCTGGCAGCATCAACTACCCTGACAAGAAGGCGCTCTCCGAAGGG CACTCGATGAGGTCGACCTGCGGTTCCACCAGGCACAGCAGCCTAGGGGACCACAAGTCCCTGGAGGCCG AGGCCCTGGCAGAAGACATCGAGAAGACCATGAGCACGGCTCTGCACGAGTTGCGGGAACTCGAGAGGCA GAACACGGTCAAGCAGGCGCCAGATGTGGTGCTGGACACCCTGGAGCCCCTGAAGAACCCGCCAGGCCCC GTCAGCTCGGAGCCCGCCAGTCCCCTTCACACCATCGTCATCCGCGACCCCGATGCCGCCATGCGCCGCA GCAGCAGCTCCTCCACCGAGATGATGACCACCTTCAAGCCAGCCCTGTCCGCCCGCCTGGCTGGCGCCCA GCTCCGCCCGCCCCCCATGCGGCCCGTGCGGCCGGTGGTCCAGCACCGGTCCAGCAGCAGCAGCAGCTCG GGCGTGGGCAGCCCGGCCGTGACGCCCACCGAGAAGATGTTCCCCAACAGCTCAGCGGACAAGTCGGGCA CCATG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG217925 representing NM_001033117
Red=Cloning site Green=Tags(s) MSSQTKFKKDKEIIAEYEAQIKEIRTQLVEQFKCLEQQSESRLQLLQDLQEFFRRKAEIELEYSRSLEKL AERFSSKIRSSREHQFKKDQYLLSPVNCWYLVLHQTRRESRDHATLNDIFMNNVIVRLSQISEDVIRLFK KSKEIGLQMHEELLKVTNELYTVMKTYHMYHAESISAESKLKEAEKQEEKQFNKSGDLSMNLLRHEDRPQ RRSSVKKIEKMKEKRQAKYSENKLKCTKARNDYLLNLAATNAAISKYYIHDVSDLIDCCDLGFHASLART FRTYLSAEYNLETSRHEGLDVIENAVDNLDSRSDKHTVMDMCNQVFCPPLKFEFQPHMGDEVCQVSAQQP VQTELLMRYHQLQSRLATLKIENEEVRKTLDATMQTLQDMLTVEDFDVSDAFQHSRSTESVKSAASETYM SKINIAKRRANQQETEMFYFTKFKEYVNGSNLITKLQAKHDLLKQTLGEGERAECGTTRGRRNARTRNQD SGQAIPLVVESCIRYINLYGLQQQGIFRVPGSQVEVNDIKNSFERGEDPLVDDQNERDINSVAGVLKLYF RGLENPLFPKERFQDLISTIKLENPAERVHQIQQILVTLPRVVIVVMRYLFAFLNHLSQYSDENMMDPYN LAICFGPTLMHIPDGQDPVSCQAHINEVIKTIIIHHEAIFPSPRELEGPVYEKCMAGGEEYCDSPHSEPG AIDEVDHDNGTEPHTSDEEVEQIEAIAKFDYMGRSPRELSFKKGASLLLYHRASEDWWEGRHNGVDGLIP HQYIVVQDMDDAFSDSLSQKADSEASSGPLLDDKASSKNDLQSPTEHISDYGFGGVMGRVRLRSDGAAIP RRRSGGDTHSPPRGLGPSIDTPPRAAACPSSPHKIPLTRGRIESPEKRRMATFGSAGSINYPDKKALSEG HSMRSTCGSTRHSSLGDHKSLEAEALAEDIEKTMSTALHELRELERQNTVKQAPDVVLDTLEPLKNPPGP VSSEPASPLHTIVIRDPDAAMRRSSSSSTEMMTTFKPALSARLAGAQLRPPPMRPVRPVVQHRSSSSSSS GVGSPAVTPTEKMFPNSSADKSGTM TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001033117 |
| ORF Size | 3225 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001033117.3 |
| RefSeq Size | 8905 bp |
| RefSeq ORF | 3228 bp |
| Locus ID | 9901 |
| UniProt ID | O43295 |
| Protein Pathways | Axon guidance |
| Gene Summary | GTPase-activating protein for RAC1 and perhaps Cdc42, but not for RhoA small GTPase. May attenuate RAC1 signaling in neurons.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217925 | SRGAP3 (Myc-DDK-tagged)-Human SLIT-ROBO Rho GTPase activating protein 3 (SRGAP3), transcript variant 2 |
CNY 10,740.00 |
|
| RC217925L3 | Lenti-ORF clone of SRGAP3 (Myc-DDK-tagged)-Human SLIT-ROBO Rho GTPase activating protein 3 (SRGAP3), transcript variant 2 |
CNY 12,640.00 |
|
| RC217925L4 | Lenti-ORF clone of SRGAP3 (mGFP-tagged)-Human SLIT-ROBO Rho GTPase activating protein 3 (SRGAP3), transcript variant 2 |
CNY 12,640.00 |
|
| SC302704 | SRGAP3 (untagged)-Human SLIT-ROBO Rho GTPase activating protein 3 (SRGAP3), transcript variant 2 |
CNY 20,710.00 |
