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Cytohesin 1 (CYTH1) (NM_017456) Human Tagged ORF Clone
CAT＃: RG218099
- TrueORF®
CYTH1 (tGFP-tagged) - Human cytohesin 1 (CYTH1), transcript variant 2
ORF Plasmid: DDK
"NM_017456" in other vectors (4)
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CNY 7,088.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | B2-1; CYTOHESIN-1; D17S811E; PSCD1; SEC7 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG218099 representing NM_017456
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAGGACGACAGCTACGTTCCCAGTGACCTGACAGCAGAGGAGCGTCAAGAACTGGAGAACATCC GACGGAGAAAACAGGAGCTGCTGGCTGACATTCAGAGGCTGAAGGATGAGATAGCAGAAGTAGCTAATGA AATTGAAAACCTGGGATCCACAGAGGAAAGGAAAAACATGCAGAGGAACAAACAGGTAGCCATGGGCAGG AAAAAATTTAATATGGACCCTAAAAAGGGGATCCAGTTCTTAATAGAGAACGACCTCCTGAAGAACACTT GTGAAGACATTGCCCAGTTCTTATATAAAGGCGAAGGGCTCAACAAGACAGCCATCGGCGACTACCTAGG GGAGAGAGATGAGTTTAATATCCAGGTTCTTCATGCATTTGTGGAGCTGCATGAGTTCACTGATCTTAAT CTCGTCCAGGCACTACGGCAGTTCCTGTGGAGCTTCCGGCTACCCGGAGAGGCCCAGAAGATCGACCGGA TGATGGAGGCGTTTGCCCAGCGATATTGTCAGTGCAATAATGGCGTGTTCCAGTCCACGGATACTTGTTA CGTCCTCTCCTTTGCCATCATCATGTTGAACACCAGTCTGCACAACCCCAATGTCAAAGATAAGCCCACT GTGGAGAGGTTCATTGCCATGAACCGAGGCATCAATGATGGGGGAGACCTGCCGGAGGAGCTCCTCCGGA ATCTCTATGAGAGCATAAAAAATGAACCCTTTAAAATCCCAGAAGACGACGGGAATGACCTCACTCACAC TTTCTTCAATCCAGACCGAGAAGGCTGGCTATTGAAACTCGGTGGCAGGGTAAAGACTTGGAAGAGACGC TGGTTCATTCTGACTGACAACTGCCTTTACTACTTTGAGTATACCACGGATAAGGAGCCCCGTGGAATCA TCCCTTTAGAGAATCTGAGTATCCGGGAAGTGGAGGACTCCAAAAAACCAAACTGCTTTGAGCTTTATAT CCCCGACAATAAAGACCAAGTTATCAAGGCCTGCAAGACCGAGGCTGACGGGCGGGTGGTGGAGGGGAAC CACACTGTTTACCGGATCTCAGCTCCGACGCCCGAGGAGAAGGAGGAGTGGATTAAGTGCATTAAAGCAG CCATCAGCAGGGACCCTTTCTACGAAATGCTCGCAGCACGGAAAAAGAAGGTCTCCTCCACGAAGCGACA C ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG218099 representing NM_017456
Red=Cloning site Green=Tags(s) MEEDDSYVPSDLTAEERQELENIRRRKQELLADIQRLKDEIAEVANEIENLGSTEERKNMQRNKQVAMGR KKFNMDPKKGIQFLIENDLLKNTCEDIAQFLYKGEGLNKTAIGDYLGERDEFNIQVLHAFVELHEFTDLN LVQALRQFLWSFRLPGEAQKIDRMMEAFAQRYCQCNNGVFQSTDTCYVLSFAIIMLNTSLHNPNVKDKPT VERFIAMNRGINDGGDLPEELLRNLYESIKNEPFKIPEDDGNDLTHTFFNPDREGWLLKLGGRVKTWKRR WFILTDNCLYYFEYTTDKEPRGIIPLENLSIREVEDSKKPNCFELYIPDNKDQVIKACKTEADGRVVEGN HTVYRISAPTPEEKEEWIKCIKAAISRDPFYEMLAARKKKVSSTKRH TRTRPLE - GFP Tag - V |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017456 |
| ORF Size | 1188 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017456.4 |
| RefSeq Size | 3363 bp |
| RefSeq ORF | 1194 bp |
| Locus ID | 9267 |
| UniProt ID | Q15438 |
| Domains | Sec7, PH |
| Gene Summary | The protein encoded by this gene is a member of the PSCD family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein activity, and the PH domain interacts with phospholipids and is responsible for association of PSCDs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. This gene is highly expressed in natural killer and peripheral T cells, and regulates the adhesiveness of integrins at the plasma membrane of lymphocytes. A pseudogene of this gene has been defined on the X chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218099 | CYTH1 (Myc-DDK-tagged)-Human cytohesin 1 (CYTH1), transcript variant 2 |
CNY 5,488.00 |
|
| RC218099L3 | Lenti ORF clone of Human cytohesin 1 (CYTH1), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC218099L4 | Lenti ORF clone of Human cytohesin 1 (CYTH1), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| SC313294 | CYTH1 (untagged)-Human cytohesin 1 (CYTH1), transcript variant 2 |
CNY 7,220.00 |
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