United States
Germany
Japan
United Kingdom
China
PI4KA (NM_058004) Human Tagged ORF Clone
CAT＃: RG218660
- TrueORF®
PI4KA (tGFP-tagged) - Human phosphatidylinositol 4-kinase, catalytic, alpha (PI4KA), transcript variant 2
ORF Plasmid: DDK
"NM_058004" in other vectors (2)
Need custom modification / cloning service?
Get a free quote
CNY 20,900.00
货期*
12周
规格
Product images
经常一起买 (4)
热销推荐Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | PI4K-ALPHA; pi4K230; PIK4CA; PMGYCHA |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RG218660 representing NM_058004
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGCCCCGGCCCGGGGAGGCGGAGGCGGAGGCGGAGGCGGCGGCGGCTGCTCCGGCTCCGGCT CCAGCGCCTCGCGGGGCTTCTATTTCAACACGGTCCTGTCACTGGCCCGCTCCCTGGCGGTGCAGAGACC AGCATCCTTGGAGAAGGTCCAAAAGCTTCTTTGCATGTGTCCAGTGGATTTCCATGGGATCTTCCAGTTA GATGAAAGACGGAGAGATGCAGTGATTGCATTGGGCATTTTTCTGATTGAATCTGATCTTCAGCACAAAG ATTGTGTGGTTCCTTACCTTCTTCGACTTCTCAAAGGTCTTCCAAAAGTGTATTGGGTAGAAGAAAGCAC AGCTCGGAAAGGCAGAGGTGCCCTCCCGGTTGCAGAGAGCTTCAGCTTCTGCTTGGTAACTCTGCTGTCT GATGTGGCCTATAGGGATCCTTCACTTAGGGATGAGATTTTAGAGGTGCTTTTGCAGGTTTTGCATGTCC TCTTGGGGATGTGCCAGGCCTTGGAGATTCAAGACAAAGAATACCTTTGCAAGTATGCTATCCCATGCCT GATAGGAATCTCGCGAGCATTTGGGCGTTACAGCAACATGGAAGAGTCTCTCCTCTCAAAGCTCTTTCCC AAAATCCCTCCTCATTCCCTCCGTGTCCTGGAAGAGCTTGAAGGTGTTCGAAGGCGTTCCTTTAATGACT TCCGCTCCATCCTCCCCAGCAATCTGCTGACTGTCTGTCAGGAGGGTACCCTGAAGAGGAAAACCAGCAG TGTGTCCAGCATCTCTCAGGTCAGCCCTGAACGCGGCATGCCCCCTCCCAGTTCCCCTGGAGGATCTGCC TTTCACTACTTTGAAGCCTCCTGCTTGCCCGATGGGACTGCCCTAGAGCCTGAGTACTACTTTTCAACCA TCAGCTCCAGCTTCTCAGTCTCTCCCCTTTTCAACGGTGTCACATATAAGGAGTTTAACATTCCATTGGA AATGCTTCGGGAACTCTTAAACCTGGTGAAGAAGATCGTTGAGGAGGCTGTTCTCAAATCTTTGGATGCC ATTGTAGCCAGTGTGATGGAGGCCAACCCCAGTGCTGATCTCTACTACACTTCCTTCAGTGACCCTCTCT ACCTGACCATGTTCAAGATGCTGCGTGACACTCTGTACTACATGAAGGACCTCCCGACCTCTTTTGTGAA GGAGATCCATGATTTTGTGCTGGAGCAGTTCAACACGAGCCAGGGGGAGCTCCAGAAGATTCTACATGAC GCAGACCGGATCCACAATGAGCTGAGCCCCCTCAAACTGCGCTGTCAGGCGAATGCTGCCTGTGTGGACC TCATGGTGTGGGCTGTGAAGGACGAGCAGGGTGCAGAAAACCTTTGCATCAAGCTATCTGAGAAGCTGCA GTCCAAGACGTCCAGCAAAGTCATTATTGCTCACTTGCCCCTGCTGATCTGCTGTCTGCAGGGTTTGGGC CGCCTGTGCGAGAGGTTCCCGGTGGTGGTGCACTCTGTGACACCGTCCTTGCGAGACTTCCTGGTCATCC CGTCCCCAGTTCTGGTGAAGCTCTACAAGTACCACAGTCAGTACCACACAGTTGCTGGCAATGATATAAA AATCAGTGTGACCAATGAGCATTCCGAGTCAACCCTGAACGTCATGTCGGGTAAGAAGAGCCAGCCCTCC ATGTACGAGCAGCTCCGAGACATCGCTATTGACAACATCTGCAGGTGCCTGAAGGCTGGATTGACGGTGG ACCCAGTGATTGTGGAGGCGTTCTTGGCCAGCCTGTCCAACCGGCTCTACATCTCTCAGGAGAGCGACAA GGACGCTCACTTGATTCCCGACCACACAATCCGAGCCTTGGGACACATTGCGGTGGCCTTGAGGGACACC CCGAAGGTCATGGAGCCCATTCTGCAGATCCTACAGCAGAAATTCTGCCAGCCACCCTCCCCCCTCGATG TGCTGATTATTGACCAGCTGGGCTGCCTGGTTATCACCGGAAATCAATACATCTATCAGGAAGTGTGGAA CCTCTTCCAGCAGATCAGTGTGAAGGCCAGCTCCGTTGTATACTCAGCCACCAAAGATTACAAGGACCAC GGCTATAGGCATTGCTCCCTGGCAGTGATTAATGCCCTGGCCAACATCGCGGCCAACATCCAAGACGAGC ACCTGGTGGATGAGCTGCTCATGAACCTGTTGGAGTTGTTTGTGCAGCTGGGGCTGGAGGGGAAGCGAGC CAGCGAGAGGGCAAGCGAGAAGGGCCCTGCCCTAAAGGCTTCTAGCAGTGCAGGGAACTTGGGAGTACTC ATTCCTGTAATAGCTGTGCTCACCCGACGACTGCCACCCATCAAAGAAGCTAAGCCTCGGTTACAGAAGC TCTTCCGAGACTTCTGGCTGTATTCCGTTCTGATGGGATTCGCTGTGGAGGGCTCAGGACTCTGGCCAGA AGAATGGTACGAGGGGGTCTGTGAAATAGCCACTAAGTCCCCCTTGCTCACCTTTCCCAGCAAGGAGCCA CTGCGGTCCGTCCTCCAGTATAACTCAGCCATGAAGAATGACACGGTCACCCCCGCTGAGCTGAGTGAGC TCCGCAGCACTATCATCAACCTGCTGGACCCCCCTCCCGAGGTGTCCGCACTCATCAACAAGCTGGACTT CGCCATGTCCACCTACCTCCTCTCTGTGTACCGGCTGGAGTACATGAGGGTACTGCGTTCAACAGATCCT GATCGCTTCCAGGTAATGTTCTGCTACTTTGAGGATAAAGCTATTCAGAAAGACAAATCTGGGATGATGC AGTGTGTGATTGCAGTCGCGGACAAAGTATTCGATGCCTTCCTGAACATGATGGCGGATAAAGCCAAGAC CAAGGAGAACGAGGAGGAGCTGGAGCGGCACGCTCAGTTCCTGTTGGTGAACTTCAACCACATCCACAAG AGGATAAGGAGGGTGGCAGACAAGTATCTATCTGGTCTGGTGGATAAGTTTCCCCACTTGCTCTGGAGCG GGACTGTGCTGAAGACCATGCTGGACATCCTGCAGACCCTGTCACTGTCACTGAGCGCTGATATTCACAA GGATCAGCCTTACTATGACATCCCCGACGCCCCCTACCGGATCACGGTTCCTGACACGTACGAAGCCCGT GAGAGCATTGTGAAGGACTTCGCTGCACGCTGTGGGATGATCCTCCAGGAGGCCATGAAGTGGGCACCTA CCGTCACCAAGTCCCACCTGCAGGAATATCTGAACAAACATCAGAACTGGGTATCGGGACTGTCCCAGCA CACGGGGCTGGCCATGGCCACTGAGAGCATCCTTCACTTTGCTGGCTACAACAAGCAGAACACAACTCTT GGGGCAACTCAGCTGAGCGAGCGCCCGGCCTGTGTGAAGAAAGACTACTCCAACTTCATGGCATCCCTGA ATCTGCGCAACCGCTACGCGGGCGAGGTGTATGGAATGATTCGGTTCTCAGGCACCACAGGCCAGATGTC TGACCTGAACAAAATGATGGTCCAGGATCTACATTCAGCTTTAGACCGCAGTCATCCTCAGCACTACACG CAGGCCATGTTCAAGCTGACCGCAATGCTCATTAGCAGTAAAGATTGTGACCCGCAGCTCCTTCATCATC TGTGCTGGGGTCCCCTCCGGATGTTCAATGAGCATGGCATGGAGACGGCCCTGGCCTGCTGGGAGTGGCT GCTGGCTGGCAAGGATGGAGTGGAAGTGCCGTTCATGCGGGAGATGGCAGGGGCCTGGCACATGACGGTG GAGCAGAAATTTGGCCTGTTTTCTGCTGAGATAAAGGAAGCAGACCCCCTGGCTGCCTCGGAAGCAAGTC AACCCAAACCCTGTCCCCCCGAAGTGACCCCCCACTACATCTGGATCGACTTCCTGGTGCAGCGGTTTGA GATCGCCAAGTACTGCAGCTCTGACCAAGTGGAGATCTTCTCCAGCCTGCTGCAGCGCTCCATGTCCCTG AACATCGGCGGGGCCAAGGGGAGCATGAACCGGCACGTGGCGGCCATCGGGCCCCGCTTCAAGCTGCTGA CCCTGGGGCTGTCCCTCCTGCATGCCGATGTGGTTCCAAATGCAACCATCCGCAATGTGCTTCGCGAGAA GATCTACTCCACTGCCTTTGACTACTTCAGCTGTCCCCCAAAGTTCCCTACTCAAGGAGAGAAGCGGCTG CGTGAAGACATAAGCATCATGATTAAATTTTGGACCGCCATGTTCTCAGATAAGAAGTACCTGACCGCCA GCCAGCTTGTTCCCCCAGATAATCAGGACACCCGGAGCAACCTGGACATAACTGTCGGCTCTCGGCAACA AGCCACCCAAGGCTGGATCAACACATACCCCCTGTCCAGCGGCATGTCCACCATCTCCAAGAAATCAGGC ATGTCTAAGAAAACCAACCGGGGCTCCCAGCTGCACAAATACTACATGAAGCGCAGGACGCTGCTGCTGT CCCTGCTGGCCACTGAGATCGAGCGTCTCATCACATGGTACAACCCGCTGTCAGCCCCGGAACTGGAACT AGACCAGGCCGGAGAGAACAGCGTGGCCAACTGGAGATCTAAGTACATCAGCCTGAGTGAGAAGCAGTGG AAGGACAACGTGAACCTCGCCTGGAGCATCTCTCCCTACCTAGCCGTGCAGCTGCCTGCCAGGTTTAAGA ACACAGAAGCCATTGGGAACGAAGTGACCCGTCTCGTTCGGTTGGACCCGGGAGCCGTTAGTGATGTGCC TGAAGCAATCAAGTTCCTGGTCACCTGGCACACCATCGACGCCGATGCTCCAGAGCTCAGCCATGTGCTG TGCTGGGCGCCCACGGACCCACCCACAGGCCTCTCCTACTTCTCCAGCATGTACCCGCCGCACCCTCTCA CGGCGCAGTACGGGGTGAAAGTCCTGCGGTCCTTCCCTCCGGACGCCATCCTCTTCTACATCCCCCAGAT TGTGCAGGCCCTCAGGTACGACAAGATGGGCTATGTGCGGGAGTATATTCTGTGGGCAGCGTCTAAATCC CAGCTTCTGGCACACCAGTTCATCTGGAACATGAAGACTAACATTTATCTAGATGAAGAGGGCCACCAGA AAGACCCTGACATCGGCGACCTCCTGGATCAGTTGGTAGAGGAGATCACAGGCTCCTTGTCCGGCCCAGC GAAGGACTTTTACCAGCGGGAGTTTGATTTCTTTAACAAGATCACCAACGTGTCGGCTATCATCAAGCCC TACCCTAAAGGCGACGAGAGAAAGAAGGCTTGTCTGTCGGCCCTGTCTGAAGTGAAGGTGCAGCCGGGCT GCTACCTGCCCAGCAACCCTGAGGCCATTGTGCTGGACATCGACTACAAGTCTGGGACCCCGATGCAGAG TGCTGCAAAAGCCCCATATCTGGCCAAGTTCAAGGTGAAGCGATGTGGAGTTAGTGAACTTGAAAAAGAA GGTCTGCGGTGCCGCTCAGACTCCGAGGATGAGTGCAGCACGCAGGAGGCCGACGGCCAGAAGATCTCCT GGCAGGCAGCCATCTTCAAGGTGGGAGACGACTGCCGGCAGGACATGCTGGCCCTGCAGATCATCGACCT CTTCAAGAACATCTTCCAGCTGGTCGGCCTGGACCTCTTTGTTTTTCCCTACCGCGTGGTGGCCACTGCC CCTGGGTGCGGGGTGATCGAGTGCATCCCCGACTGCACCTCCCGGGACCAGCTGGGCCGCCAGACAGACT TCGGCATGTACGACTACTTCACACGCCAGTACGGGGATGAGTCCACTCTGGCCTTCCAGCAGGCCCGCTA CAACTTCATCCGAAGCATGGCCGCCTACAGCCTCCTGCTGTTCCTGCTGCAGATCAAGGACAGACACAAC GGCAACATTATGCTGGACAAGAAGGGTCATATCATCCACATCGACTTTGGCTTCATGTTTGAAAGCTCGC CGGGCGGCAATCTCGGCTGGGAACCCGACATCAAGCTGACGGATGAGATGGTGATGATCATGGGGGGCAA GATGGAGGCCACACCCTTCAAGTGGTTCATGGAGATGTGTGTCCGAGGCTACCTGGCTGTGCGGCCCTAC ATGGACGCGGTCGTCTCCCTGGTCACTCTCATGTTGGACACGGGCCTGCCCTGTTTTCGCGGCCAGACAA TCAAGCTCTTGAAGCACAGGTTTAGCCCCAACATGACTGAGCGCGAGGCTGCAAATTTCATCATGAAGGT CATCCAGAGCTGCTTCCTCAGCAACAGGAGCCGGACCTACGACATGATCCAGTACTATCAGAATGACATC CCCTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >RG218660 representing NM_058004
Red=Cloning site Green=Tags(s) MAAAPARGGGGGGGGGGGCSGSGSSASRGFYFNTVLSLARSLAVQRPASLEKVQKLLCMCPVDFHGIFQL DERRRDAVIALGIFLIESDLQHKDCVVPYLLRLLKGLPKVYWVEESTARKGRGALPVAESFSFCLVTLLS DVAYRDPSLRDEILEVLLQVLHVLLGMCQALEIQDKEYLCKYAIPCLIGISRAFGRYSNMEESLLSKLFP KIPPHSLRVLEELEGVRRRSFNDFRSILPSNLLTVCQEGTLKRKTSSVSSISQVSPERGMPPPSSPGGSA FHYFEASCLPDGTALEPEYYFSTISSSFSVSPLFNGVTYKEFNIPLEMLRELLNLVKKIVEEAVLKSLDA IVASVMEANPSADLYYTSFSDPLYLTMFKMLRDTLYYMKDLPTSFVKEIHDFVLEQFNTSQGELQKILHD ADRIHNELSPLKLRCQANAACVDLMVWAVKDEQGAENLCIKLSEKLQSKTSSKVIIAHLPLLICCLQGLG RLCERFPVVVHSVTPSLRDFLVIPSPVLVKLYKYHSQYHTVAGNDIKISVTNEHSESTLNVMSGKKSQPS MYEQLRDIAIDNICRCLKAGLTVDPVIVEAFLASLSNRLYISQESDKDAHLIPDHTIRALGHIAVALRDT PKVMEPILQILQQKFCQPPSPLDVLIIDQLGCLVITGNQYIYQEVWNLFQQISVKASSVVYSATKDYKDH GYRHCSLAVINALANIAANIQDEHLVDELLMNLLELFVQLGLEGKRASERASEKGPALKASSSAGNLGVL IPVIAVLTRRLPPIKEAKPRLQKLFRDFWLYSVLMGFAVEGSGLWPEEWYEGVCEIATKSPLLTFPSKEP LRSVLQYNSAMKNDTVTPAELSELRSTIINLLDPPPEVSALINKLDFAMSTYLLSVYRLEYMRVLRSTDP DRFQVMFCYFEDKAIQKDKSGMMQCVIAVADKVFDAFLNMMADKAKTKENEEELERHAQFLLVNFNHIHK RIRRVADKYLSGLVDKFPHLLWSGTVLKTMLDILQTLSLSLSADIHKDQPYYDIPDAPYRITVPDTYEAR ESIVKDFAARCGMILQEAMKWAPTVTKSHLQEYLNKHQNWVSGLSQHTGLAMATESILHFAGYNKQNTTL GATQLSERPACVKKDYSNFMASLNLRNRYAGEVYGMIRFSGTTGQMSDLNKMMVQDLHSALDRSHPQHYT QAMFKLTAMLISSKDCDPQLLHHLCWGPLRMFNEHGMETALACWEWLLAGKDGVEVPFMREMAGAWHMTV EQKFGLFSAEIKEADPLAASEASQPKPCPPEVTPHYIWIDFLVQRFEIAKYCSSDQVEIFSSLLQRSMSL NIGGAKGSMNRHVAAIGPRFKLLTLGLSLLHADVVPNATIRNVLREKIYSTAFDYFSCPPKFPTQGEKRL REDISIMIKFWTAMFSDKKYLTASQLVPPDNQDTRSNLDITVGSRQQATQGWINTYPLSSGMSTISKKSG MSKKTNRGSQLHKYYMKRRTLLLSLLATEIERLITWYNPLSAPELELDQAGENSVANWRSKYISLSEKQW KDNVNLAWSISPYLAVQLPARFKNTEAIGNEVTRLVRLDPGAVSDVPEAIKFLVTWHTIDADAPELSHVL CWAPTDPPTGLSYFSSMYPPHPLTAQYGVKVLRSFPPDAILFYIPQIVQALRYDKMGYVREYILWAASKS QLLAHQFIWNMKTNIYLDEEGHQKDPDIGDLLDQLVEEITGSLSGPAKDFYQREFDFFNKITNVSAIIKP YPKGDERKKACLSALSEVKVQPGCYLPSNPEAIVLDIDYKSGTPMQSAAKAPYLAKFKVKRCGVSELEKE GLRCRSDSEDECSTQEADGQKISWQAAIFKVGDDCRQDMLALQIIDLFKNIFQLVGLDLFVFPYRVVATA PGCGVIECIPDCTSRDQLGRQTDFGMYDYFTRQYGDESTLAFQQARYNFIRSMAAYSLLLFLLQIKDRHN GNIMLDKKGHIIHIDFGFMFESSPGGNLGWEPDIKLTDEMVMIMGGKMEATPFKWFMEMCVRGYLAVRPY MDAVVSLVTLMLDTGLPCFRGQTIKLLKHRFSPNMTEREAANFIMKVIQSCFLSNRSRTYDMIQYYQNDI PY TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_058004 |
| ORF Size | 6306 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_058004.4 |
| RefSeq Size | 6768 bp |
| RefSeq ORF | 6309 bp |
| Locus ID | 5297 |
| UniProt ID | P42356 |
| Domains | PI3_PI4_kinase, PI3Ka |
| Protein Families | Druggable Genome |
| Protein Pathways | Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system |
| Gene Summary | This gene encodes a phosphatidylinositol (PI) 4-kinase which catalyzes the first committed step in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. The mammalian PI 4-kinases have been classified into two types, II and III, based on their molecular mass, and modulation by detergent and adenosine. The protein encoded by this gene is a type III enzyme that is not inhibited by adenosine. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Apr 2018] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
You may also need
Customer
Reviews
Loading...
