DMP1 (NM_004407) Human Tagged ORF Clone

CAT#: RG218741

  • TrueORF®

DMP1 (tGFP-tagged) - Human dentin matrix acidic phosphoprotein 1 (DMP1), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_004407" in other vectors (6)

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CNY 5,424.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


Goat Anti-DMP1 Antibody
    • 100 ug

CNY 5,371.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Synonyms ARHP; ARHR; DMP-1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG218741 representing NM_004407
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGATCAGCATCCTGCTCATGTTCCTTTGGGGATTATCCTGTGCTCTCCCAGTAACCAGGTATCAAA
ATAATGAATCTGAGGATTCTGAAGAATGGAAGGGTCATTTGGCTCAGGCACCAACACCACCCTTGGAGAG
CAGTGAGTCATCAGAAGGCAGTAAAGTTAGCTCAGAGGAACAGGCAAATGAAGACCCCAGTGACAGCACT
CAGTCAGAGGAGGGCCTGGGCTCTGATGATCATCAATACATTTATAGGCTAGCTGGTGGCTTCTCCAGGA
GCACAGGAAAAGGAGGAGATGATAAAGATGACGATGAAGATGACAGTGGAGATGACACCTTTGGTGACGA
TGACAGTGGCCCAGGGCCCAAAGACAGACAAGAAGGAGGAAACTCCAGACTGGGAAGTGATGAGGACTCT
GATGACACCATACAAGCCAGTGAAGAGAGTGCCCCACAAGGGCAAGACAGTGCCCAAGATACCACCAGTG
AGAGCAGGGAACTTGACAATGAGGACCGGGTGGACAGCAAGCCTGAGGGAGGTGACTCCACTCAAGAGAG
TGAGAGTGAAGAGCACTGGGTGGGAGGTGGCAGTGATGGGGAGAGCAGCCATGGAGACGGCTCCGAGTTG
GACGATGAGGGAATGCAGAGTGATGACCCAGAGAGCATCAGGAGTGAAAGGGGAAACTCCAGAATGAACA
GTGCAGGCATGAAATCAAAAGAATCTGGAGAAAACAGTGAGCAAGCAAACACTCAAGATTCAGGTGGCAG
CCAATTGCTGGAGCATCCCAGTAGGAAAATTTTTAGGAAGTCTCGCATCTCAGAGGAAGATGACAGAAGC
GAGCTTGATGACAACAACACAATGGAAGAAGTCAAGAGTGACTCTACAGAAAACAGCAACTCCAGAGACA
CTGGCCTCAGCCAACCCAGGAGAGACAGCAAGGGTGACTCTCAAGAAGACAGCAAGGAGAATCTGTCCCA
GGAAGAGAGCCAAAACGTAGATGGTCCCAGCAGTGAGTCCAGCCAAGAGGCCAACCTGTCATCTCAAGAG
AACAGCAGTGAGTCTCAGGAAGAGGTGGTGAGTGAGTCCAGGGGAGATAACCCCGACCCCACAACTAGTT
ATGTAGAAGACCAGGAAGACAGTGACTCCAGCGAGGAGGACAGCTCGCACACACTCTCCCACTCAAAAAG
TGAATCCAGAGAGGAGCAAGCAGACAGCGAATCCAGTGAGAGCCTCAACTTCTCAGAGGAAAGCCCGGAG
TCCCCTGAGGATGAGAACAGCTCCAGCCAGGAGGGCCTCCAGTCTCACAGCAGCTCAGCAGAGAGTCAGA
GCGAGGAAAGCCATTCTGAGGAAGACGACAGTGACTCTCAAGACAGCAGCAGATCCAAAGAAGATAGCAA
CTCCACGGAGAGCAAATCAAGCAGTGAGGAAGATGGCCAGTTGAAAAACATTGAGATAGAGAGCCGGAAA
TTAACAGTTGATGCCTATCACAACAAACCCATTGGGGACCAAGATGACAATGACTGCCAAGACGGCTAT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG218741 representing NM_004407
Red=Cloning site Green=Tags(s)

MKISILLMFLWGLSCALPVTRYQNNESEDSEEWKGHLAQAPTPPLESSESSEGSKVSSEEQANEDPSDST
QSEEGLGSDDHQYIYRLAGGFSRSTGKGGDDKDDDEDDSGDDTFGDDDSGPGPKDRQEGGNSRLGSDEDS
DDTIQASEESAPQGQDSAQDTTSESRELDNEDRVDSKPEGGDSTQESESEEHWVGGGSDGESSHGDGSEL
DDEGMQSDDPESIRSERGNSRMNSAGMKSKESGENSEQANTQDSGGSQLLEHPSRKIFRKSRISEEDDRS
ELDDNNTMEEVKSDSTENSNSRDTGLSQPRRDSKGDSQEDSKENLSQEESQNVDGPSSESSQEANLSSQE
NSSESQEEVVSESRGDNPDPTTSYVEDQEDSDSSEEDSSHTLSHSKSESREEQADSESSESLNFSEESPE
SPEDENSSSQEGLQSHSSSAESQSEESHSEEDDSDSQDSSRSKEDSNSTESKSSSEEDGQLKNIEIESRK
LTVDAYHNKPIGDQDDNDCQDGY

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_004407
ORF Size 1539 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004407.4
RefSeq Size 2682 bp
RefSeq ORF 1542 bp
Locus ID 1758
UniProt ID Q13316
Protein Families Secreted Protein
Gene Summary Dentin matrix acidic phosphoprotein is an extracellular matrix protein and a member of the small integrin binding ligand N-linked glycoprotein family. This protein, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. The protein contains a large number of acidic domains, multiple phosphorylation sites, a functional arg-gly-asp cell attachment sequence, and a DNA binding domain. In undifferentiated osteoblasts it is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in the gene are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia. The gene structure is conserved in mammals. Two transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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